TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2835489.2.1
(885 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN820177.1|CN820177 HRO4412_A07_B14ZS5 Lib AA069E1X Aven... 341 e-120
gb|CN816985.1|CN816985 HRO4527_A03_B05ZS5 Lib AA071E1X Aven... 418 e-118
>gb|CN820177.1|CN820177 HRO4412_A07_B14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4412_A07_B14, mRNA
sequence
Length = 675
Score = 341 bits (738), Expect(2) = e-120
Identities = 140/170 (82%), Positives = 154/170 (90%)
Frame = -3 / +1
Query: 586 LGTSILEAIQELVGVQTEVVYEKCPTEAMIETGGFSYAVVVVGEVPYAEWTGDRTDLSIP 407
LGTSILEAIQE VGV+TEVV+E+CPT+A+IETGGFSYAVVVVGEVPYAEW GDRTDLSIP
Sbjct: 142 LGTSILEAIQESVGVETEVVHEQCPTDAIIETGGFSYAVVVVGEVPYAEWLGDRTDLSIP 321
Query: 406 FNGSDLIIRVASKIPTLVIVISGRPLIIESQVLEKIEALVAAWLPGSEGMGITDCLFGDH 227
FNGSDLI RVASK+PTLVIVISGRPL+I+ QVLEKI+ALVAAWLPGSEG GITDC+FGDH
Sbjct: 322 FNGSDLITRVASKVPTLVIVISGRPLVIDPQVLEKIDALVAAWLPGSEGNGITDCIFGDH 501
Query: 226 DFVGTLPVTWCKSVDQLPIDAGDSNYDPLFPVGYGLKMFRSDNDST*LYY 77
DFVGTLPVTW + DQ PI+ GD+ YDPLFP GYGLKMFRSD S * Y+
Sbjct: 502 DFVGTLPVTWFRFSDQFPINIGDAKYDPLFPFGYGLKMFRSDKGSP*PYH 651
Score = 292 bits (633), Expect(2) = e-101
Identities = 134/171 (78%), Positives = 144/171 (84%)
Frame = +3 / -2
Query: 72 NK**SYVESLSLRNIFSPYPTGNSGS*LESPASIGN*STDLHHVTGNVPTKS*SPKRQSV 251
N+** Y E LSL NIF+P P GN GS L SP IGN*S +L+HVTGNVPTKS SPK QSV
Sbjct: 656 NQ**GYGEPLSLLNIFNP*PKGNKGSYLASPIFIGN*SENLNHVTGNVPTKSWSPKMQSV 477
Query: 252 IPMPSLPGSQAATRASIFSKTCDSIINGLPDITITRVGILLATRMIRSEPLNGILRSVLS 431
IP+PSLPGS AATRASIFS TC SI GLPD+TIT VG LLATR+IRSEPL GILRSVLS
Sbjct: 476 IPLPSLPGSHAATRASIFSSTCGSITKGLPDMTITSVGTLLATRVIRSEPLKGILRSVLS 297
Query: 432 PVHSAYGTSPTTTTA*ENPPVSIIASVGHFSYTTSVCTPTNSCMASNMLVP 584
P HSA GTSPTTTTA*ENPPVS+IASVGH S TTSV TPT+SCMAS+MLVP
Sbjct: 296 PNHSA*GTSPTTTTA*ENPPVSMIASVGHCSCTTSVSTPTDSCMASSMLVP 144
Score = 237 bits (513), Expect(2) = 3e-083
Identities = 104/175 (59%), Positives = 122/175 (69%)
Frame = -2 / +3
Query: 596 KNNSWYEHIGGHTRISGSAN*SCV*EMPNRSYD*NWGVFLCGGGCWRGSICRMDRR*N*P 417
K +WY+H GHTRI S N*SC * MPNR Y *NW +FLC CWRGS+C M R*N*P
Sbjct: 132 KITAWYKHTRGHTRIRRSGN*SCA*AMPNRCYH*NWRIFLCSCCCWRGSLC*MVGR*N*P 311
Query: 416 *YSV*WL*PYHPCCK*NPYPSDCYIWKAINY*VTSFGKDRSSSRCLAAWK*GHGNYRLPL 237
*YS+ W P + C *+PY SDC+IWKA+ Y* TS GKDR SS C+A WK*G NY L L
Sbjct: 312 *YSLQWFRPDYSGC**SPYTSDCHIWKALGY*STSTGKDRCSSCCVATWK*GQRNY*LHL 491
Query: 236 WRS*FCGHIACDMV*VC*SIAYRCWRL*L*PAIPCWIWAKNVSKR**FNITLLFI 72
W *FC HIAC++V*V *SI+Y+ WR +*P I W+W +NV K * F ITL +
Sbjct: 492 WGP*FCWHIACNVV*VF*SISYKYWRCQI*PLISFWLWVENV*K**RFPITLSLV 656
Score = 214 bits (462), Expect(2) = 5e-077
Identities = 106/174 (60%), Positives = 120/174 (68%)
Frame = +1 / -3
Query: 76 NNKVMLNHYRFETFLAHIQQGIAGHS*SLQHL*AIDQQTYTMSQAMCPQNHDLQRGNR*F 255
N+KVM N Y F TF H Q+ I GH LQ+L* IDQ+T T QAMC QNH QR N+*F
Sbjct: 652 NDKVMGNLYHF*TFSTHSQKEIRGHIWHLQYL*EIDQKT*TTLQAMCQQNHGPQRCNQ*F 473
Query: 256 PCPHFQAARQRLELLSFPKLVTQ*LMAFQI*QSLG*GFYLQHG**GQSH*TEY*GQFYLL 435
CPHFQ A Q+LE LSFP LV Q* AFQI*QSL *G Y Q * G++H* EY*GQFYL
Sbjct: 472 RCPHFQVATQQLEHLSFPVLVDQ*PRAFQI*QSLV*GLY*QPE*SGRNH*REY*GQFYLP 293
Query: 436 SIRHMEPLQQPPPHRKTPQFQS*LRLGISHTQLQFALPLILVWPPICSYQELFF 597
+I+H EP QQ H+K QFQ * LGI+H QLQF L ILVWP +C YQ + F
Sbjct: 292 TIQHKEPRQQQQLHKKILQFQ**HLLGIAHAQLQFPLRRILVWPLVCLYQAVIF 131
Score = 202 bits (436), Expect(2) = 9e-071
Identities = 95/160 (59%), Positives = 114/160 (71%)
Frame = +2 / -1
Query: 107 SKHF*PISNRE*RVIVRVSSIYRQLINRLTPCHRQCAHKIMISKEAIGNSHALTSRQPGS 286
SKHF PI+ R+* VI +S+IYR+LI + P +RQCA+KIM+ K+AI NS ALTSR P S
Sbjct: 621 SKHFQPIAKRK*GVIFGISNIYRKLIRKPKPRYRQCANKIMVPKDAISNSVALTSR*PRS 442
Query: 287 D*SFYLFQXXXXXXXWPSRYNNH*GRDFTCNTDDKVRAIKRNTKVSSISCPFGIWNLSNN 466
+*S YLFQ PSRY+NH* RDFT N ++V I+ NTKVSSIS PF I NL+NN
Sbjct: 441 N*SIYLFQYLWINNQGPSRYDNH*CRDFTSNPSNQVGTIEGNTKVSSISQPFSIRNLANN 262
Query: 467 HHRIGKPPSFNHSFGWAFLIHNFSLHSH*FLYGLQYARTK 586
++ I K SFN S WA L+HNFS HS FLYGL YA TK
Sbjct: 261 NNCIRKSSSFNDSICWALLMHNFSFHSDGFLYGL*YACTK 142
Score = 107 bits (229), Expect(3) = 3e-061
Identities = 53/81 (65%), Positives = 57/81 (70%)
Frame = -1 / +2
Query: 318 HKFWKR*KL*SLPGCLEVRAWELPIASLEIMILWAHCL*HGVSLLINCL*MLETLTMTRY 139
HK+WKR* L* L G LEVRA EL IASL MIL AHCL* G+ LIN L*+LE MT Y
Sbjct: 410 HKYWKR*ML*LLRGYLEVRATELLIASLGTMILLAHCL*RGLGFLINFL*ILEMPNMTPY 589
Query: 138 SLLDMG*KCFEAIMIQHNFII 76
LL MG*KC E I + HN II
Sbjct: 590 FLLAMG*KCLEVIKVPHNLII 652
Score = 107 bits (228), Expect(2) = e-120
Identities = 41/46 (89%), Positives = 44/46 (95%)
Frame = -3 / +3
Query: 724 KDQNKPFLPLAKDAKRILVAGTHADDIGYQCGGWTIAWHGDSGKIT 587
K+Q + FLPLAK+AKRILVAGTHADDIGYQCGGWTIAWHGDSGKIT
Sbjct: 3 KNQKEMFLPLAKNAKRILVAGTHADDIGYQCGGWTIAWHGDSGKIT 140
Score = 91.3 bits (193), Expect(2) = e-101
Identities = 35/46 (76%), Positives = 38/46 (82%)
Frame = +2 / -2
Query: 587 SYFSTVSMPCYRPATTLISNIISMCSCNQYSFCIFGQWKEWLILIL 724
SY STVSMP Y P T LISNI+SMC CN+YSFCIFGQWKE +LIL
Sbjct: 140 SYLSTVSMPSYCPPTALISNIVSMCPCNEYSFCIFGQWKEHFLLIL 3
Score = 89.0 bits (188), Expect(2) = 5e-077
Identities = 40/50 (80%), Positives = 40/50 (80%)
Frame = +3 / -3
Query: 576 LVPRVIFPLSPCHAIVQPPH*YPISSACVPATSILFASLANGRNGLF*SL 725
L VIFPLSPC AIV PPH*YPISSACVPATSI FA LANGRN F* L
Sbjct: 151 LYQAVIFPLSPCQAIVHPPH*YPISSACVPATSIRFAFLANGRNISF*FL 2
Score = 86.3 bits (182), Expect(2) = 3e-083
Identities = 31/46 (67%), Positives = 39/46 (84%)
Frame = -2 / +2
Query: 725 QGSE*AIPSIGQRCKKNTGCRNTC**YWISMWWLDDSMAWRQWKNN 588
Q SE +PSIGQ+CK NT CR+TC* YWIS+ W+D+S+AWRQWK+N
Sbjct: 2 QESEGNVPSIGQKCKTNTRCRDTC*RYWISVRWVDNSLAWRQWKDN 139
Score = 83.5 bits (176), Expect(3) = 3e-061
Identities = 47/75 (62%), Positives = 49/75 (65%)
Frame = -1 / +2
Query: 585 LVRAYWRPYKN*WECKLKLCMRNAQPKL*LKLGGFPMRWWLLERFHMPNGQEIELTLVFR 406
LV+AY RPYKN E KLKLCM NAQ L LKL F M+ LL RF M NG EIELTLVF
Sbjct: 143 LVQAY*RPYKNPSEWKLKLCMSNAQQMLSLKLEDFLMQLLLLARFLMLNGWEIELTLVFP 322
Query: 405 LMALTLSSVLQVKSL 361
M T L VKSL
Sbjct: 323 SMVPT*LLGLLVKSL 367
Score = 79.9 bits (168), Expect(2) = 9e-071
Identities = 31/48 (64%), Positives = 34/48 (70%)
Frame = +1 / -1
Query: 583 QELFFHCLHAMLSSSHHIDIQYHQHVFLQPVFFLHLWPMEGMAYSDPC 726
++L FHCLHA L S+H DIQY QHV LQ VF LH WPMEG SD C
Sbjct: 144 KQLSFHCLHAKLLSTHRTDIQYRQHVSLQRVFVLHFWPMEGTFPSDSC 1
Score = 78.0 bits (164), Expect(3) = 3e-061
Identities = 35/48 (72%), Positives = 38/48 (79%)
Frame = -1 / +1
Query: 726 ARIRISHSFHWPKMQKEYWLQEHMLMILDINVVAGR*HGMETVEK*LL 583
ARIR SFHWPKMQ EY LQ HML ILDI+ V G+* GMETVE+*LL
Sbjct: 1 ARIRRKCSFHWPKMQNEYSLQGHMLTILDISAVGGQ*LGMETVER*LL 144
>gb|CN816985.1|CN816985 HRO4527_A03_B05ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4527_A03_B05, mRNA sequence
Length = 608
Score = 418 bits (906), Expect = e-118
Identities = 168/200 (84%), Positives = 187/200 (93%)
Frame = -3 / +1
Query: 733 KNGKDQNKPFLPLAKDAKRILVAGTHADDIGYQCGGWTIAWHGDSGKITLGTSILEAIQE 554
++GK+ + FLPLAK+AKRILVAGTHADDIGYQCGGWTIAWHGDSGKITLGTSILEAIQE
Sbjct: 7 EDGKNLKETFLPLAKNAKRILVAGTHADDIGYQCGGWTIAWHGDSGKITLGTSILEAIQE 186
Query: 553 LVGVQTEVVYEKCPTEAMIETGGFSYAVVVVGEVPYAEWTGDRTDLSIPFNGSDLIIRVA 374
VGV+TEVV+E+CPT+A+IETGGFSYAVVVVGEVPYAEW GDRTDLSIPFNGSDLI RVA
Sbjct: 187 SVGVETEVVHEQCPTDAIIETGGFSYAVVVVGEVPYAEWLGDRTDLSIPFNGSDLITRVA 366
Query: 373 SKIPTLVIVISGRPLIIESQVLEKIEALVAAWLPGSEGMGITDCLFGDHDFVGTLPVTWC 194
SK+PTLVIVISGRPL+I+ QV+EKI+ALVAAWLPGSEG GITDC+FGDHDFVGTLPVTW
Sbjct: 367 SKVPTLVIVISGRPLVIDQQVMEKIDALVAAWLPGSEGTGITDCIFGDHDFVGTLPVTWF 546
Query: 193 KSVDQLPIDAGDSNYDPLFP 134
+S DQLPI+ GD+ YD LFP
Sbjct: 547 RSSDQLPINIGDAKYDLLFP 606
Score = 353 bits (765), Expect = 2e-098
Identities = 161/198 (81%), Positives = 168/198 (84%)
Frame = +3 / -1
Query: 135 GNSGS*LESPASIGN*STDLHHVTGNVPTKS*SPKRQSVIPMPSLPGSQAATRASIFSKT 314
GN S L SP IGN*S DL+HVTGNVPTKS SPK QSVIP+PSLPGS AATRASIFS T
Sbjct: 605 GNKRSYLASPIFIGN*SEDLNHVTGNVPTKSWSPKMQSVIPVPSLPGSHAATRASIFSIT 426
Query: 315 CDSIINGLPDITITRVGILLATRMIRSEPLNGILRSVLSPVHSAYGTSPTTTTA*ENPPV 494
C SI GLPD+TIT VG LLATR+IRSEPL GILRSVLSP HSA GTSPTTTTA*ENPPV
Sbjct: 425 C*SITKGLPDMTITSVGTLLATRVIRSEPLKGILRSVLSPNHSA*GTSPTTTTA*ENPPV 246
Query: 495 SIIASVGHFSYTTSVCTPTNSCMASNMLVPRVIFPLSPCHAIVQPPH*YPISSACVPATS 674
S+IASVGH S TTSV TPT+SCMAS+MLVPRVIFPLSPC AIV PPH*YPISSACVPATS
Sbjct: 245 SMIASVGHCSCTTSVSTPTDSCMASSMLVPRVIFPLSPCQAIVHPPH*YPISSACVPATS 66
Query: 675 ILFASLANGRNGLF*SLP 728
I FA LANGRN F LP
Sbjct: 65 IRFAFLANGRNVSFRFLP 12
Score = 316 bits (685), Expect = 3e-087
Identities = 130/197 (65%), Positives = 151/197 (76%)
Frame = -2 / +3
Query: 728 WQGSE*AIPSIGQRCKKNTGCRNTC**YWISMWWLDDSMAWRQWKNNSWYEHIGGHTRIS 549
WQ SE +PSIGQ+CK NT CR+TC* YWIS+ W+D+S+AWRQWK+NSWY+H GGHTRI
Sbjct: 12 WQESEGNVPSIGQKCKTNTCCRDTC*RYWISVRWVDNSLAWRQWKDNSWYKHTGGHTRIR 191
Query: 548 GSAN*SCV*EMPNRSYD*NWGVFLCGGGCWRGSICRMDRR*N*P*YSV*WL*PYHPCCK* 369
S N*SC * MPNR Y *NW +FLC CWRGS+C M R*N*P*YS+*W P + C *
Sbjct: 192 RSGN*SCA*AMPNRCYH*NWRIFLCSCCCWRGSLC*MVGR*N*P*YSL*WFRPDYSGC** 371
Query: 368 NPYPSDCYIWKAINY*VTSFGKDRSSSRCLAAWK*GHGNYRLPLWRS*FCGHIACDMV*V 189
+PY SDC+IWKA+ Y* TS GKDR SS C+A WK*GH NY L LW *FC HIAC++V*V
Sbjct: 372 SPYTSDCHIWKALGY*STSNGKDRCSSCCVATWK*GHRNY*LHLWGP*FCWHIACNVV*V 551
Query: 188 C*SIAYRCWRL*L*PAI 138
*SIAY+ WR +*P I
Sbjct: 552 F*SIAYKYWRCQI*PLI 602
Score = 285 bits (617), Expect = 6e-078
Identities = 128/199 (64%), Positives = 146/199 (73%)
Frame = +2 / -3
Query: 134 RE*RVIVRVSSIYRQLINRLTPCHRQCAHKIMISKEAIGNSHALTSRQPGSD*SFYLFQX 313
R+* VI +S+IYRQLI R P +RQCA+KIM+ K+AI NS ALTSR P S+*S YLF
Sbjct: 606 RK*EVIFGISNIYRQLIRRPKPRYRQCANKIMVPKDAISNSGALTSR*PRSN*SIYLFHY 427
Query: 314 XXXXXXWPSRYNNH*GRDFTCNTDDKVRAIKRNTKVSSISCPFGIWNLSNNHHRIGKPPS 493
PSRY+NH* RDFT N ++V IK NTKVSSIS PF I NL+NN++ I K S
Sbjct: 426 LLINNQGPSRYDNH*CRDFTSNPSNQVGTIKGNTKVSSISQPFSIRNLANNNNCIRKSSS 247
Query: 494 FNHSFGWAFLIHNFSLHSH*FLYGLQYARTKSYFSTVSMPCYRPATTLISNIISMCSCNQ 673
FN S WA L+HNFS HS FLYGLQYA TKSY STVSMP Y P T LISNI+SMC CN+
Sbjct: 246 FNDSICWALLMHNFSFHSDGFLYGLQYACTKSYLSTVSMPSYCPPTALISNIVSMCPCNK 67
Query: 674 YSFCIFGQWKEWLILILAI 730
YSFCIFGQWKE + ILAI
Sbjct: 66 YSFCIFGQWKERFLQILAI 10
Score = 278 bits (602), Expect = 8e-076
Identities = 129/197 (65%), Positives = 141/197 (71%)
Frame = +1 / -2
Query: 139 IAGHS*SLQHL*AIDQQTYTMSQAMCPQNHDLQRGNR*FPCPHFQAARQRLELLSFPKLV 318
I GH LQ+L*AIDQ+T T QAMC QNH QR N+*F CPHFQ A Q+LE LSFP LV
Sbjct: 601 IRGHIWHLQYL*AIDQKT*TTLQAMCQQNHGPQRCNQ*FRCPHFQVATQQLEHLSFPLLV 422
Query: 319 TQ*LMAFQI*QSLG*GFYLQHG**GQSH*TEY*GQFYLLSIRHMEPLQQPPPHRKTPQFQ 498
Q* AFQI*QSL *G Y Q * G++H* EY*GQFYL +I+H EP QQ H+K QFQ
Sbjct: 421 DQ*PRAFQI*QSLV*GLY*QPE*SGRNH*REY*GQFYLPTIQHKEPRQQQQLHKKILQFQ 242
Query: 499 S*LRLGISHTQLQFALPLILVWPPICSYQELFFHCLHAMLSSSHHIDIQYHQHVFLQPVF 678
* LGI+H QLQF L ILVWPP+C YQEL FHCLHA L S+H DIQY QHV LQ VF
Sbjct: 241 **HLLGIAHAQLQFPLRRILVWPPVCLYQELSFHCLHAKLLSTHRTDIQYRQHVSLQQVF 62
Query: 679 FLHLWPMEGMAYSDPCH 729
LH WPMEG SD CH
Sbjct: 61 VLHFWPMEGTFPSDSCH 11
Score = 167 bits (359), Expect(2) = 2e-060
Identities = 83/124 (66%), Positives = 89/124 (71%)
Frame = -1 / +2
Query: 732 KMARIRISHSFHWPKMQKEYWLQEHMLMILDINVVAGR*HGMETVEK*LLVRAYWRPYKN 553
+MARI SFHWPKMQ EY LQ HML ILDI+ V G+* GMETVE+*LLV+AYWRPYKN
Sbjct: 8 RMARI*RKRSFHWPKMQNEYLLQGHMLTILDISAVGGQ*LGMETVER*LLVQAYWRPYKN 187
Query: 552 *WECKLKLCMRNAQPKL*LKLGGFPMRWWLLERFHMPNGQEIELTLVFRLMALTLSSVLQ 373
E KLKLCM NAQ L LKL F M+ LL RF M NG EIELTLVF LM T L
Sbjct: 188 PSEWKLKLCMSNAQQMLSLKLEDFLMQLLLLARFLMLNGWEIELTLVFPLMVPT*LLGLL 367
Query: 372 VKSL 361
VKSL
Sbjct: 368 VKSL 379
Score = 80.3 bits (169), Expect(2) = 2e-060
Identities = 42/62 (67%), Positives = 45/62 (72%)
Frame = -1 / +2
Query: 318 HKFWKR*KL*SLPGCLEVRAWELPIASLEIMILWAHCL*HGVSLLINCL*MLETLTMTRY 139
+K WKR* L* L G LEVRA EL IASL MIL AHCL* G+ LLINCL*+LE MT Y
Sbjct: 422 NK*WKR*ML*LLRGYLEVRAPELLIASLGTMILLAHCL*RGLGLLINCL*ILEMPNMTSY 601
Query: 138 SL 133
L
Sbjct: 602 FL 607
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,350,488
Number of Sequences: 8143
Number of extensions: 177593
Number of successful extensions: 1077
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 305551838
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)