TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2619548.2.1
(691 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815714.1|CN815714 HRO4514_G01_M02ZS5 Lib AA071E1X Aven... 297 2e-081
gb|CN815860.1|CN815860 HRO4511_C09_F17ZS5 Lib AA071E1X Aven... 274 1e-074
gb|CN821620.1|CN821620 HRO4408_D02_H04Z Lib AA069E1X Avena ... 219 5e-058
gb|CN816546.1|CN816546 HRO4522_A04_A08ZS5 Lib AA071E1X Aven... 199 4e-052
gb|CN820862.1|CN820862 HRO4420_G10_N20ZS5 Lib AA069E1X Aven... 140 2e-034
>gb|CN815714.1|CN815714 HRO4514_G01_M02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_G01_M02, mRNA sequence
Length = 643
Score = 297 bits (642), Expect = 2e-081
Identities = 125/138 (90%), Positives = 130/138 (94%)
Frame = +2 / +3
Query: 56 AKMAVPLLTQKIVKKRVKQFKRPHLDRYKCLKPSWRRPKGIDSRVRRKFKGCTLMPNIGY 235
++MAVPLLT KIVKKR K FKR H DRY LKPSWRRPKGIDSRVRRKFKGCTLMPNIGY
Sbjct: 30 SEMAVPLLTAKIVKKRTKHFKRAHSDRYIGLKPSWRRPKGIDSRVRRKFKGCTLMPNIGY 209
Query: 236 GSDKKTRHYLPNKFKKFVVHNVSELELLLMHNRTYCAEIAHNVSTRKRKEIVERAAQLDI 415
GSDKKTRHYLPNKFKKFVVHNVSELELL+MHNRTYCAEIAHNVS++KRK IVERAAQLDI
Sbjct: 210 GSDKKTRHYLPNKFKKFVVHNVSELELLMMHNRTYCAEIAHNVSSQKRKVIVERAAQLDI 389
Query: 416 VVTNKLARLRSQEDE*NV 469
VVTNKLARLRSQEDE*NV
Sbjct: 390 VVTNKLARLRSQEDE*NV 443
Score = 153 bits (328), Expect(2) = 2e-044
Identities = 69/127 (54%), Positives = 88/127 (69%)
Frame = -1 / -3
Query: 502 FNTSRAQSKLLNISLVLLAAEPGELVGDHDVQLRSTLHNLLALAGGHVVGDLSAVSPVVH 323
+ + S L NISL+LLAAEPG+LV D+D+QLRSTLHN L L G +V DLS + PVVH
Sbjct: 476 YKQRKHNSSLKNISLILLAAEPGKLVCDNDIQLRSTLHNDLTLLGRDIVRDLSTICPVVH 297
Query: 322 QQQLQLGDIVNNKLFEXXXXXXXXXXXGAISDVGHQGASLELTPDAGVDTLGPAPAWLKA 143
+QLQL D+V++KL E +++VGHQG SLEL+P GV+TL PAPA L+A
Sbjct: 296 HKQLQLRDVVDDKLLELVGQVMPGLLVRTVTNVGHQGTSLELSPYTGVNTLWPAPAGLEA 117
Query: 142 LVAVKMG 122
VA+ MG
Sbjct: 116 DVAIAMG 96
Score = 132 bits (283), Expect = 6e-032
Identities = 65/115 (56%), Positives = 68/115 (59%)
Frame = +3 / +1
Query: 123 PILTATSALSQAGAGPRVSTPASGVSSRDAP*CPTSDMAPTRRPGTTFPTSSKSLLFTMS 302
PI ATSA S AGAG RV TP G SSRD P*CPT TRRPG T PTSS+SL T S
Sbjct: 97 PIAIATSASSPAGAGQRVLTPV*GESSRDVP*CPTLVTVLTRRPGITCPTSSRSLSSTTS 276
Query: 303 PSWSCC*CTTGLTALRSPTTCPPASARRLWSVLRNXXXXXXXXXXXXXXKRTSEM 467
SWSC *CTTG LRS T P S R LWSVLR+ +R SEM
Sbjct: 277 LSWSCL*CTTGHIVLRSRTMSLPRSVRSLWSVLRSWISLSQTSLPGSAARRMSEM 441
Score = 122 bits (260), Expect = 8e-029
Identities = 60/99 (60%), Positives = 64/99 (64%)
Frame = -2 / -1
Query: 468 TFHXXXXXXXXXXXLVTTMSSCAARSTISLRLRVDTLWAISAQ*VLLCIXXXXXSEXXXX 289
TFH VTT+SSCAARST++LR +TL AISAQ VLLCI SE
Sbjct: 442 TFHSSSWLRSLASLFVTTISSCAARSTMTLRFWEETLCAISAQYVLLCIISNSSSETLWT 263
Query: 288 XXXXXXLGR*CLVFLSEPYPMLGIKVHPLNLRLTRESIP 172
LGR*CLVFLSEP PMLGIKVHPLN LTRESIP
Sbjct: 262 TNFLNLLGR*CLVFLSEP*PMLGIKVHPLNFLLTRESIP 146
Score = 87.2 bits (184), Expect(2) = 2e-031
Identities = 35/58 (60%), Positives = 44/58 (75%)
Frame = -3 / -2
Query: 296 CEQQTF*TCWEGSAWSSCRSHIRCWASRCIP*TYA*RGSRYPWACASLA*GTCSGQDG 123
C +QT *TCW G+AWSSC++ +CWASR IP*T++ GS+YP A AS A*G CS + G
Sbjct: 270 CGRQTS*TCWAGNAWSSCQNRNQCWASRYIP*TFSLHGSQYPLAGASWA*GRCSDRYG 97
Score = 62.9 bits (131), Expect(2) = 2e-031
Identities = 24/38 (63%), Positives = 26/38 (68%)
Frame = -3 / -2
Query: 470 QHFTRPLGCGAWRACW*PRCPVAQHAPQSPCACGWTRC 357
+HFT P GCGAW+AC * R P AQHAPQ P A G C
Sbjct: 444 EHFTHPPGCGAWQACL*QRYPAAQHAPQ*PYASGKRHC 331
Score = 40.9 bits (83), Expect(2) = 2e-044
Identities = 16/22 (72%), Positives = 16/22 (72%)
Frame = -2 / -1
Query: 126 WGLLNCLTLFFTIFCVSSGTAI 61
W LL CL FFTIF VS GTAI
Sbjct: 100 WALLKCLVRFFTIFAVSRGTAI 35
>gb|CN815860.1|CN815860 HRO4511_C09_F17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_C09_F17, mRNA sequence
Length = 587
Score = 274 bits (592), Expect = 1e-074
Identities = 115/126 (91%), Positives = 119/126 (94%)
Frame = +2 / +2
Query: 92 VKKRVKQFKRPHLDRYKCLKPSWRRPKGIDSRVRRKFKGCTLMPNIGYGSDKKTRHYLPN 271
VKKRVKQFKR H DRY LK SWRRPKGIDSRVRRKFKG TLMPNIGYGSDKKTRHYLPN
Sbjct: 11 VKKRVKQFKRAHSDRYIGLKTSWRRPKGIDSRVRRKFKGVTLMPNIGYGSDKKTRHYLPN 190
Query: 272 KFKKFVVHNVSELELLLMHNRTYCAEIAHNVSTRKRKEIVERAAQLDIVVTNKLARLRSQ 451
KFKKFVVHN+SELELL+MHNRTYCAEIAHNVST+KRK+IVERAAQLDIVVTNKLARLRSQ
Sbjct: 191 KFKKFVVHNISELELLMMHNRTYCAEIAHNVSTKKRKDIVERAAQLDIVVTNKLARLRSQ 370
Query: 452 EDE*NV 469
EDE* V
Sbjct: 371 EDE*TV 388
Score = 129 bits (277), Expect = 4e-031
Identities = 64/108 (59%), Positives = 70/108 (64%)
Frame = -2 / -3
Query: 495 PAEHNQNYSTFHXXXXXXXXXXXLVTTMSSCAARSTISLRLRVDTLWAISAQ*VLLCIXX 316
P + + N T + LVTTMSSCAARST+SLR V+TLWAISAQ*VLLCI
Sbjct: 414 PYQKHSNSRTVYSSSWLRSLASLLVTTMSSCAARSTMSLRFFVETLWAISAQ*VLLCIMS 235
Query: 315 XXXSEXXXXXXXXXXLGR*CLVFLSEPYPMLGIKVHPLNLRLTRESIP 172
SE LGR*CLVFLS+P PMLGI V PLN RLTRESIP
Sbjct: 234 SSSSEMLWTTNFLNLLGR*CLVFLSDP*PMLGINVTPLNFRLTRESIP 91
Score = 121 bits (259), Expect = 1e-028
Identities = 61/119 (51%), Positives = 68/119 (57%)
Frame = +3 / +3
Query: 123 PILTATSALSQAGAGPRVSTPASGVSSRDAP*CPTSDMAPTRRPGTTFPTSSKSLLFTMS 302
PI TAT A AGAG RV P SG SSR++ *CPT TRRPG TF TSS+S L T
Sbjct: 42 PIATATLASRLAGAGRRVLIPVSGESSRESR*CPTLATGQTRRPGITFLTSSRSSLSTTF 221
Query: 303 PSWSCC*CTTGLTALRSPTTCPPASARRLWSVLRNXXXXXXXXXXXXXXKRTSEMLSSF 479
SWSC *CTTG T RSPTT P SAR SVLR+ +R S + S+
Sbjct: 222 LSWSCS*CTTGPTVRRSPTTSPRRSARTSLSVLRSLTSWSPTSLPGSAARRMSRLFLSY 398
Score = 102 bits (217), Expect(3) = 4e-047
Identities = 44/67 (65%), Positives = 55/67 (82%)
Frame = -1 / -2
Query: 475 LLNISLVLLAAEPGELVGDHDVQLRSTLHNLLALAGGHVVGDLSAVSPVVHQQQLQLGDI 296
L N L+LLAAEPG+LVGDHDV+LRSTL+++LAL G VVGDL V PVVH +QLQL ++
Sbjct: 394 LKNSLLILLAAEPGKLVGDHDVKLRSTLNDVLALLRGDVVGDLRTVGPVVHHEQLQLRNV 215
Query: 295 VNNKLFE 275
V+N+L E
Sbjct: 214 VDNELLE 194
Score = 85.8 bits (181), Expect(3) = 4e-047
Identities = 35/58 (60%), Positives = 45/58 (77%)
Frame = -3 / -1
Query: 296 CEQQTF*TCWEGSAWSSCRSHIRCWASRCIP*TYA*RGSRYPWACASLA*GTCSGQDG 123
C Q+T *TC EG+AWSSC + +CWAS +P*T+A* G++YP ACAS +*G CSG+ G
Sbjct: 215 CGQRTS*TC*EGNAWSSCLTRSQCWAST*LP*TFA*HGNQYPSACAS*S*GQCSGRYG 42
Score = 79.0 bits (166), Expect(2) = 3e-027
Identities = 27/43 (62%), Positives = 30/43 (69%)
Frame = -3 / -1
Query: 482 IKTTQHFTRPLGCGAWRACW*PRCPVAQHAPQSPCACGWTRCG 354
I T + T P GCGAW+ACW*PRC AQHA + PCA W RCG
Sbjct: 401 IVTQEQSTHPPGCGAWQACW*PRCQAAQHAQRCPCASSWRRCG 273
Score = 57.4 bits (119), Expect(2) = 3e-027
Identities = 24/38 (63%), Positives = 31/38 (81%)
Frame = -1 / -2
Query: 235 ISDVGHQGASLELTPDAGVDTLGPAPAWLKALVAVKMG 122
+++VGHQ SLEL+PD G++TL PAPA L+A VAV MG
Sbjct: 154 VANVGHQRDSLELSPDTGINTLRPAPASLEANVAVAMG 41
Score = 33.1 bits (66), Expect(3) = 4e-047
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = -2 / -3
Query: 126 WGLLNCLTLFFTI 88
W LLNCLTLFFT+
Sbjct: 45 WALLNCLTLFFTL 7
>gb|CN821620.1|CN821620 HRO4408_D02_H04Z Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4408_D02_H04, mRNA
sequence
Length = 477
Score = 219 bits (472), Expect = 5e-058
Identities = 91/97 (93%), Positives = 96/97 (98%)
Frame = +2 / +2
Query: 179 DSRVRRKFKGCTLMPNIGYGSDKKTRHYLPNKFKKFVVHNVSELELLLMHNRTYCAEIAH 358
DSRVRRKFKG TLMPNIGYGSDKKTRHYLPNKFKKFVVHN+SELELL+MHNRTYCAEIAH
Sbjct: 11 DSRVRRKFKGVTLMPNIGYGSDKKTRHYLPNKFKKFVVHNISELELLMMHNRTYCAEIAH 190
Query: 359 NVSTRKRKEIVERAAQLDIVVTNKLARLRSQEDE*NV 469
NVST+KRK+IVERAAQLDIVVTNKLARLRSQEDE*+V
Sbjct: 191 NVSTKKRKDIVERAAQLDIVVTNKLARLRSQEDE*SV 301
Score = 125 bits (267), Expect = 9e-030
Identities = 62/106 (58%), Positives = 68/106 (64%)
Frame = -2 / -1
Query: 495 PAEHNQNYSTFHXXXXXXXXXXXLVTTMSSCAARSTISLRLRVDTLWAISAQ*VLLCIXX 316
P + + N T + LVTTMSSCAARST+SLR V+TLWAISAQ*VLLCI
Sbjct: 327 PYQKHSNSRTLYSSSWLRSLASLLVTTMSSCAARSTMSLRFFVETLWAISAQ*VLLCIMS 148
Query: 315 XXXSEXXXXXXXXXXLGR*CLVFLSEPYPMLGIKVHPLNLRLTRES 178
SE LGR*CLVFLS+P PMLGI V PLN RLTRES
Sbjct: 147 SSSSEMLWTTNFLNLLGR*CLVFLSDP*PMLGINVTPLNFRLTRES 10
Score = 104 bits (222), Expect(2) = 8e-036
Identities = 45/67 (67%), Positives = 55/67 (82%)
Frame = -1 / -3
Query: 475 LLNISLVLLAAEPGELVGDHDVQLRSTLHNLLALAGGHVVGDLSAVSPVVHQQQLQLGDI 296
L N L+LLAAEPG+LVGDHDV+LRSTL+++LAL G VVG+L V PVVH +QLQL D+
Sbjct: 307 LKNTLLILLAAEPGKLVGDHDVKLRSTLNDVLALLRGDVVGNLRTVGPVVHHEQLQLRDV 128
Query: 295 VNNKLFE 275
V+NKL E
Sbjct: 127 VDNKLLE 107
Score = 103 bits (219), Expect = 4e-023
Identities = 50/99 (50%), Positives = 57/99 (57%)
Frame = +3 / +3
Query: 183 PASGVSSRDAP*CPTSDMAPTRRPGTTFPTSSKSLLFTMSPSWSCC*CTTGLTALRSPTT 362
P SG SSR++ *CPT TRRPG TF TSS+SLL T S SWSC *CTTG T R PTT
Sbjct: 15 PVSGESSRESH*CPTLATGQTRRPGITFLTSSRSLLSTTSLSWSCS*CTTGPTVRRLPTT 194
Query: 363 CPPASARRLWSVLRNXXXXXXXXXXXXXXKRTSEMLSSF 479
P SAR SVLR+ +R S + S+
Sbjct: 195 SPRRSARTSLSVLRSLTSWSPTSLPGSAARRMSRVFLSY 311
Score = 60.6 bits (126), Expect(2) = 8e-036
Identities = 24/39 (61%), Positives = 30/39 (76%)
Frame = -3 / -2
Query: 296 CEQQTF*TCWEGSAWSSCRSHIRCWASRCIP*TYA*RGS 180
C QQT *TC EG+AWSSC + +CWAS +P*T+A* G+
Sbjct: 128 CGQQTS*TC*EGNAWSSCLTRSQCWASM*LP*TFA*HGN 12
>gb|CN816546.1|CN816546 HRO4522_A04_A08ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4522_A04_A08, mRNA sequence
Length = 639
Score = 199 bits (429), Expect = 4e-052
Identities = 84/88 (95%), Positives = 86/88 (97%)
Frame = +2 / +1
Query: 206 GCTLMPNIGYGSDKKTRHYLPNKFKKFVVHNVSELELLLMHNRTYCAEIAHNVSTRKRKE 385
G TLMPNIGYGSDKKTRHYLPNKFKKFVVHNVSELELL+MHNRTYCAEIAHNVST+KRK
Sbjct: 1 GVTLMPNIGYGSDKKTRHYLPNKFKKFVVHNVSELELLMMHNRTYCAEIAHNVSTQKRKT 180
Query: 386 IVERAAQLDIVVTNKLARLRSQEDE*NV 469
IVERAAQLDIVVTNKLARLRSQEDE*NV
Sbjct: 181 IVERAAQLDIVVTNKLARLRSQEDE*NV 264
Score = 118 bits (252), Expect = 1e-027
Identities = 54/88 (61%), Positives = 61/88 (69%)
Frame = -1 / -1
Query: 469 NISLVLLAAEPGELVGDHDVQLRSTLHNLLALAGGHVVGDLSAVSPVVHQQQLQLGDIVN 290
++SLVLLAAEPGELVGDHDV+L TLH LAL GHVV DL V PVVH +QLQL DIV+
Sbjct: 264 DVSLVLLAAEPGELVGDHDVELGGTLHYRLALLRGHVVCDLGTVRPVVHHKQLQLRDIVH 85
Query: 289 NKLFEXXXXXXXXXXXGAISDVGHQGAS 206
+KL E +SDVGHQG S
Sbjct: 84 HKLLELVGQVVPGLLVRTVSDVGHQGDS 1
Score = 111 bits (237), Expect = 1e-025
Identities = 50/86 (58%), Positives = 55/86 (63%)
Frame = +3 / +2
Query: 207 DAP*CPTSDMAPTRRPGTTFPTSSKSLLFTMSPSWSCC*CTTGLTALRSPTTCPPASARR 386
++P*CPTSD TRRPGTT PTSS+SL TMS SWSC *CTTG T RS TTCP SARR
Sbjct: 2 ESP*CPTSDTVLTRRPGTTCPTSSRSLWCTMSLSWSCL*CTTGRTVPRSHTTCPRRSARR 181
Query: 387 LWSVLRNXXXXXXXXXXXXXXKRTSE 464
WSV + +RTSE
Sbjct: 182 *WSVPPSSTSWSPTSSPGSAARRTSE 259
Score = 102 bits (218), Expect = 5e-023
Identities = 54/86 (62%), Positives = 54/86 (62%)
Frame = -2 / -2
Query: 468 TFHXXXXXXXXXXXLVTTMSSCAARSTISLRLRVDTLWAISAQ*VLLCIXXXXXSEXXXX 289
TFH LVTTMSS AARSTI LR VDTL AISAQ VLLCI SE
Sbjct: 263 TFHSSSWLRSLASLLVTTMSSWAARSTIVLRFCVDTLCAISAQYVLLCIISSSSSETLCT 84
Query: 288 XXXXXXLGR*CLVFLSEPYPMLGIKV 211
LGR*CLVFLSEPYPMLGIKV
Sbjct: 83 TNFLNLLGR*CLVFLSEPYPMLGIKV 6
Score = 80.3 bits (169), Expect(2) = 1e-029
Identities = 27/38 (71%), Positives = 29/38 (76%)
Frame = -3 / -3
Query: 470 QHFTRPLGCGAWRACW*PRCPVAQHAPQSPCACGWTRC 357
+ FTRP GCGAWRACW*PRC +HAP S CA WTRC
Sbjct: 265 RRFTRPPGCGAWRACW*PRCRAGRHAPLSSCASAWTRC 152
Score = 64.3 bits (134), Expect(2) = 1e-029
Identities = 22/29 (75%), Positives = 24/29 (82%)
Frame = -3 / -3
Query: 299 HCEQQTF*TCWEGSAWSSCRSHIRCWASR 213
HC QT *TCW GSAWSSC++ IRCWASR
Sbjct: 94 HCAPQTS*TCWAGSAWSSCQNRIRCWASR 8
>gb|CN820862.1|CN820862 HRO4420_G10_N20ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4420_G10_N20, mRNA
sequence
Length = 427
Score = 140 bits (301), Expect = 2e-034
Identities = 60/65 (92%), Positives = 64/65 (98%)
Frame = +2 / +3
Query: 275 FKKFVVHNVSELELLLMHNRTYCAEIAHNVSTRKRKEIVERAAQLDIVVTNKLARLRSQE 454
FKKF++HNVSELELL+MHNRTYCAEIAHNVST+KRK IVERAAQLDIVVTNKLARLRSQE
Sbjct: 3 FKKFLLHNVSELELLMMHNRTYCAEIAHNVSTQKRKTIVERAAQLDIVVTNKLARLRSQE 182
Query: 455 DE*NV 469
DE*NV
Sbjct: 183 DE*NV 197
Score = 106 bits (227), Expect = 3e-024
Identities = 47/65 (72%), Positives = 51/65 (78%)
Frame = -1 / -3
Query: 469 NISLVLLAAEPGELVGDHDVQLRSTLHNLLALAGGHVVGDLSAVSPVVHQQQLQLGDIVN 290
N+SLVLLAAEPGELVGDHDV+L TLH LAL GHV+ DLS V PVVH +QLQL DIV
Sbjct: 197 NVSLVLLAAEPGELVGDHDVELGGTLHYRLALLSGHVMCDLSTVRPVVHHKQLQLRDIVQ 18
Query: 289 NKLFE 275
KL E
Sbjct: 17 QKLLE 3
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,997,038
Number of Sequences: 8143
Number of extensions: 85710
Number of successful extensions: 1250
Number of sequences better than 1.0e-020: 6
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 229164947
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)