TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2542159.2.1
(1231 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF084005.1|AF084005 Avena fatua ras-like small monomeric... 255 7e-098
gb|CN815005.1|CN815005 HRO4504_G12_N24ZS5 Lib AA071E1X Aven... 183 4e-047
>gb|AF084005.1|AF084005 Avena fatua ras-like small monomeric GTP-binding protein (SAR1)
mRNA, complete cds
Length = 801
Score = 255 bits (551), Expect = 1e-068
Identities = 114/194 (58%), Positives = 140/194 (72%)
Frame = -1 / -3
Query: 727 LDVLRHPFEAFAIAHLAHHTAHEDLQGPDIGVGQVHVALPGCEVAQAEVVPQLLL*SRVW 548
+DVL P E IAHLA +TAHE L+ PDIGV + +ALP CE A+A V+PQL +W
Sbjct: 742 IDVLGDPLEPITIAHLADNTAHEHLKRPDIGVTKADLALPSCEAAKA*VIPQLFFRGSIW 563
Query: 547 DVNLVAQYEERNVCKGVICKKRIELLLRLGKTFLVNSIHQVHNSINLGVVVLPDAAGDLV 368
D+NL+A+ E+ +VC+G+I K+ IELL R K LV I+QV+ SINL VV+ PD+A DLV
Sbjct: 562 DINLIAKNEKWDVCQGIIRKESIELLFRFSKALLVVCIYQVYYSINLSVVIFPDSASDLV 383
Query: 367 APKVERLELDPADAQLFXXXXXXXXXLYEPLVLQHVEEGGLAGVVEAKEEDLSLLLPQPK 188
A +VE ELD ADA+L L+EPLVL+HVEE GLAGVVEAKEEDLSLLLPQ +
Sbjct: 382 ASEVEGFELDLADAELLRRWVLRGLVLHEPLVLEHVEERGLAGVVEAKEEDLSLLLPQAE 203
Query: 187 RCQHPIEPVHQEHL 146
R QHP+EPV EHL
Sbjct: 202 RGQHPVEPVP*EHL 161
Score = 194 bits (418), Expect(2) = 7e-098
Identities = 79/82 (96%), Positives = 81/82 (98%)
Frame = +2 / +2
Query: 146 KMFLVDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQYPTSEEL 325
KMFL DWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQ+PTSEEL
Sbjct: 161 KMFLWDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEEL 340
Query: 326 SIGRIKFKAFDLGGHQIARRVW 391
SIG+IKFKAFDLGGHQIARRVW
Sbjct: 341 SIGKIKFKAFDLGGHQIARRVW 406
Score = 187 bits (403), Expect(2) = 6e-080
Identities = 75/84 (89%), Positives = 78/84 (92%)
Frame = -2 / -1
Query: 393 FQTRRAIWWPPRSNALNLILPMLNSSDVGYCVGWCCTSRSSFSMWRRVVLPALSRPRKRI 214
FQTRRAIWWPPRS ALNLILPML+SSDVG CVGWCCTSRSS SMWR VVLPALSRPRKRI
Sbjct: 408 FQTRRAIWWPPRSKALNLILPMLSSSDVGCCVGWCCTSRSSLSMWRSVVLPALSRPRKRI 229
Query: 213 LASFCHSPSDASTP*NQSTRNIFT 142
LASFCH PS+ASTP*NQS RNIF+
Sbjct: 228 LASFCHRPSEASTP*NQSHRNIFS 157
Score = 179 bits (385), Expect(2) = 7e-098
Identities = 74/94 (78%), Positives = 80/94 (85%)
Frame = +2 / +2
Query: 449 ERFAESKKEXXXXXXXXXXXNVPFLILGNKIDIPYAASEEELRYYLGLSNFTTGKGNVNL 628
ERFAESKKE NVPFLILGNKIDIPYAASEEELRY+LGLS+FTTGKG V+L
Sbjct: 464 ERFAESKKELDALLSDDSLANVPFLILGNKIDIPYAASEEELRYHLGLSSFTTGKGKVSL 643
Query: 629 ADSNVRPLEIFMCSVVRKMGYGEGFKWMSQYIK* 730
DSNVRPLE+FMCSVVRKMGYG+GFKW+SQYI *
Sbjct: 644 CDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIN* 745
Score = 126 bits (269), Expect(2) = 6e-080
Identities = 55/72 (76%), Positives = 59/72 (81%)
Frame = -2 / -1
Query: 726 LMY*DIHLKPSP*PILRTTLHMKISRGRTLESAKFTLPFPVVKLLRPR*YLXXXSEAAYG 547
LMY + HL PSP*PILRTTLHM SRGRTLES + TLPFPVVKLLRP+*Y SEAAYG
Sbjct: 741 LMYWETHLNPSP*PILRTTLHMNTSRGRTLESQRLTLPFPVVKLLRPK*YRSSSSEAAYG 562
Query: 546 MSILLPSMRKGT 511
+SILLP MR GT
Sbjct: 561 ISILLPRMRNGT 526
Score = 89.5 bits (189), Expect(3) = 3e-041
Identities = 45/91 (49%), Positives = 56/91 (61%)
Frame = +1 / +1
Query: 457 CRVEEGARCASCR*LPCKRSFPHTGQQD*HPIRGFRGGAEVLPRPEQLHNREGQRELGRL 636
C +E+GARC+ * P KR H+ Q D*+PI A V RP QLHN EGQ + L
Sbjct: 472 C*IEKGARCSPF**FPGKRPISHSWQ*D*YPICCL*RRAAVSLRP*QLHNWEGQGQPL*L 651
Query: 637 QCPAPGDLHVQCGAQDGLWRRLQMDVSVHQV 729
QCPA +HVQC QDGLW +Q+ + VHQ+
Sbjct: 652 QCPAS*GVHVQCCPQDGLW*WVQVGLPVHQL 744
Score = 68.4 bits (143), Expect(3) = 3e-041
Identities = 28/43 (65%), Positives = 32/43 (74%)
Frame = +1 / +1
Query: 265 AEGRAARTAPADAVPHVRRVEHRQDQVQGVRPWGPPDRPPRLE 393
A+GRAAR APA A P+V +HRQDQVQ +RP PPDR P LE
Sbjct: 280 AQGRAARAAPAHAAPNVGGAQHRQDQVQSLRPRRPPDRSPSLE 408
Score = 44.6 bits (91), Expect(3) = 3e-041
Identities = 20/26 (76%), Positives = 22/26 (84%)
Frame = +1 / +1
Query: 145 KDVPGGLVLWGAGIAWAVAEGG*DPL 222
+DVP GLVL GAG+A VAEGG*DPL
Sbjct: 160 EDVPMGLVLRGAGLARPVAEGG*DPL 237
>gb|CN815005.1|CN815005 HRO4504_G12_N24ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4504_G12_N24, mRNA sequence
Length = 633
Score = 183 bits (395), Expect = 4e-047
Identities = 76/94 (80%), Positives = 81/94 (86%)
Frame = +2 / +3
Query: 449 ERFAESKKEXXXXXXXXXXXNVPFLILGNKIDIPYAASEEELRYYLGLSNFTTGKGNVNL 628
ERFAESKKE NVPFLILGNKIDIPYAASEEELRY+LGLSNFTTGKG V+L
Sbjct: 72 ERFAESKKELDALLSDDSLANVPFLILGNKIDIPYAASEEELRYHLGLSNFTTGKGKVSL 251
Query: 629 ADSNVRPLEIFMCSVVRKMGYGEGFKWMSQYIK* 730
DSNVRPLE+FMCSVVRKMGYG+GFKW+SQYIK*
Sbjct: 252 GDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIK* 353
Score = 140 bits (300), Expect = 5e-034
Identities = 61/110 (55%), Positives = 78/110 (70%)
Frame = -1 / -2
Query: 727 LDVLRHPFEAFAIAHLAHHTAHEDLQGPDIGVGQVHVALPGCEVAQAEVVPQLLL*SRVW 548
LDVL P E IAHLA +TAHE L+ PDIGV + +ALP CEVA+A V+PQL +W
Sbjct: 350 LDVLGDPLEPITIAHLADNTAHEHLKRPDIGVTKADLALPSCEVAKA*VIPQLFFRGSIW 171
Query: 547 DVNLVAQYEERNVCKGVICKKRIELLLRLGKTFLVNSIHQVHNSINLGVV 398
D+NL+A+ E+ +VC+G+I K+ IELL R K LV I+QV+ SINL VV
Sbjct: 170 DINLIAKNEKWDVCQGIIRKESIELLFRFSKALLVVCIYQVYYSINLSVV 21
Score = 127 bits (271), Expect = 5e-030
Identities = 55/73 (75%), Positives = 60/73 (82%)
Frame = -2 / -3
Query: 729 HLMY*DIHLKPSP*PILRTTLHMKISRGRTLESAKFTLPFPVVKLLRPR*YLXXXSEAAY 550
+LMY + HL PSP*PILRTTLHM SRGRTLES + TLPFPVVKLLRP+*Y SEAAY
Sbjct: 352 YLMYWETHLNPSP*PILRTTLHMNTSRGRTLESPRLTLPFPVVKLLRPK*YRSSSSEAAY 173
Query: 549 GMSILLPSMRKGT 511
G+SILLP MR GT
Sbjct: 172 GISILLPRMRNGT 134
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,020,236
Number of Sequences: 8143
Number of extensions: 181882
Number of successful extensions: 1029
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 441356175
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)