TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2521825.2.1
         (2812 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN817408.1|CN817408  HRO4454_E10_J20ZS5 Lib AA070E1X Aven...   116   2e-026

>gb|CN817408.1|CN817408 HRO4454_E10_J20ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
           Avena sativa cDNA clone HRO4454_E10_J20, mRNA sequence
          Length = 484

 Score =  116 bits (248), Expect = 2e-026
 Identities = 49/57 (85%), Positives = 50/57 (87%)
 Frame = +2 / -3

                                                                    
Query: 527 QVRGGERTLNEFGRLLKFVSGTPGTPSGDVK*STSWPTLPCPPKTRASESRATLYFP 697
           QVRGG+R LNEFGRL KFVSGTPGTPSG VK*STSWPTLPCPP  RASESRATL  P
Sbjct: 182 QVRGGDRILNEFGRL*KFVSGTPGTPSGVVK*STSWPTLPCPPNIRASESRATLSSP 12




 Score =  115 bits (245), Expect = 5e-026
 Identities = 52/108 (48%), Positives = 66/108 (61%)
 Frame = -2 / +1

                                                                       
Query: 696 GKYRVALDSDALXXXXXXXXXXXXXXFTSPEGVPGVPETNFNNRPNSFKVLSPPRTCVAY 517
           G+ RVALDSDAL              FT+PEGVPGVPETNF+NRPNSF++LSPPRTCV Y
Sbjct: 13  GEDRVALDSDALMFGGHGRVGHDVDHFTTPEGVPGVPETNFHNRPNSFRILSPPRTCVVY 192

                                                           
Query: 516 YRVDEAGAGRRLHAKAETGKTSPAESIDVKASRASSKEDKEATAGGKK 373
           Y VDE    R+      + ++     IDV+A+      D +A +G +K
Sbjct: 193 YCVDEKAERRQKEEAFSSEESMAVGYIDVQATPVKGTTDGKAASGSRK 336



  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 35,221,169
Number of Sequences: 8143
Number of extensions: 568835
Number of successful extensions: 2799
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 46
effective length of database: 1,192,909
effective search space used: 1061689010
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)