TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2478075.2.1
(807 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY277385.1| Avena sativa 1,3-beta glucanase (Oglc13) gen... 85 9e-027
gb|AF155932.1| Avena sativa 1,3-beta glucanase (Oglc13) mRN... 81 1e-025
emb|Z15131.1|ASBGLUCAN A.sativa mRNA for beta glucanase 66 2e-024
gb|CN821029.1|CN821029 HRO4422_G03_M06ZS5 Lib AA069E1X Aven... 75 4e-022
>gb|AY277385.1| Avena sativa 1,3-beta glucanase (Oglc13) gene, complete cds
Length = 3281
Score = 84.9 bits (179), Expect(3) = 9e-027
Identities = 33/69 (47%), Positives = 42/69 (60%)
Frame = +3 / +2
Query: 255 GAVYTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGEGXXXXXXXXXXXXXXXXX 434
G YTN+FDA+VDA HAA+EKAG G+++VVSE+GWP GG G
Sbjct: 2837 GLTYTNLFDAMVDAVHAALEKAGAPGVKVVVSESGWPKTGGTGASTDNARAYNQGLIDHV 3016
Query: 435 DGGTPRRPG 461
GGTP++ G
Sbjct: 3017 GGGTPKKRG 3043
Score = 34.5 bits (69), Expect(3) = 9e-027
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +3 / +2
Query: 516 GVEQHFGLFQPDMSEVYHVDF 578
G E+HFGLF PD S Y + F
Sbjct: 3098 GTEKHFGLFNPDKSPAYPIRF 3160
Score = 33.1 bits (66), Expect(3) = 9e-027
Identities = 14/24 (58%), Positives = 17/24 (70%)
Frame = +3 / +2
Query: 438 GGTPRRPGKALETYLFAMFNENGK 509
GG + ALETY+FAMF+EN K
Sbjct: 3017 GGGTPKKRGALETYVFAMFDENQK 3088
>gb|AF155932.1| Avena sativa 1,3-beta glucanase (Oglc13) mRNA, partial cds
Length = 1045
Score = 81.3 bits (171), Expect(3) = 1e-025
Identities = 32/69 (46%), Positives = 41/69 (59%)
Frame = +3 / +1
Query: 255 GAVYTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGEGXXXXXXXXXXXXXXXXX 434
G YTN+FDA+VDA HAA+EKAG +++VVSE+GWP GG G
Sbjct: 592 GLTYTNLFDAMVDAVHAALEKAGAASVKVVVSESGWPKEGGTGASVDNARAYNQGLIDHV 771
Query: 435 DGGTPRRPG 461
GGTP++ G
Sbjct: 772 GGGTPKKRG 798
Score = 34.5 bits (69), Expect(3) = 1e-025
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +3 / +1
Query: 516 GVEQHFGLFQPDMSEVYHVDF 578
G E+HFGLF PD S Y + F
Sbjct: 853 GTEKHFGLFNPDKSPAYPIRF 915
Score = 33.1 bits (66), Expect(3) = 1e-025
Identities = 14/24 (58%), Positives = 17/24 (70%)
Frame = +3 / +1
Query: 438 GGTPRRPGKALETYLFAMFNENGK 509
GG + ALETY+FAMF+EN K
Sbjct: 772 GGGTPKKRGALETYVFAMFDENQK 843
>emb|Z15131.1|ASBGLUCAN A.sativa mRNA for beta glucanase
Length = 1448
Score = 66.1 bits (138), Expect(2) = 2e-024
Identities = 27/50 (54%), Positives = 35/50 (70%)
Frame = +3 / +1
Query: 231 AASTVTDGGAVYTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGE 380
+ + V DG Y N+FD VDA + A+ K G G++LVVSE+GWPSAGGE
Sbjct: 685 SGTVVQDGAYGYQNLFDTTVDAFYTAMGKHGGAGVKLVVSESGWPSAGGE 834
Score = 61.1 bits (127), Expect(2) = 2e-024
Identities = 23/37 (62%), Positives = 29/37 (78%)
Frame = +3 / +1
Query: 468 LETYLFAMFNENGKAEGVEQHFGLFQPDMSEVYHVDF 578
+ETY+FAMFNEN K GVEQ++GLF P+M VY + F
Sbjct: 919 IETYVFAMFNENQKDNGVEQNWGLFYPNMQHVYPISF 1029
>gb|CN821029.1|CN821029 HRO4422_G03_M06ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4422_G03_M06, mRNA
sequence
Length = 508
Score = 74.8 bits (157), Expect(3) = 4e-022
Identities = 30/75 (40%), Positives = 41/75 (54%)
Frame = +3 / +3
Query: 237 STVTDGGAVYTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGEGXXXXXXXXXXX 416
+ V DG Y N+FDA+VD ++A+ AG +++VVSE+GWPSAGG
Sbjct: 12 TVVQDGSNAYQNLFDALVDTFYSALGSAGAGNVKIVVSESGWPSAGGTAASAANAQTYNQ 191
Query: 417 XXXXXXDGGTPRRPG 461
GTP+RPG
Sbjct: 192 NLINHVGKGTPKRPG 236
Score = 33.1 bits (66), Expect(3) = 4e-022
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +3 / +3
Query: 465 ALETYLFAMFNENGK 509
A+ETY+FAMFNEN K
Sbjct: 237 AIETYIFAMFNENKK 281
Score = 29.0 bits (57), Expect(3) = 4e-022
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = +3 / +3
Query: 522 EQHFGLFQPDMSEVYHVDF 578
E+HFGLF D S Y + F
Sbjct: 297 EKHFGLFNTDKSPAYSIKF 353
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,340,040
Number of Sequences: 8143
Number of extensions: 71320
Number of successful extensions: 740
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 273901050
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)