TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441542.2.1
(3782 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Aven... 220 2e-057
gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Aven... 194 8e-050
gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Aven... 182 2e-046
gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Aven... 115 5e-026
>gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C09_F17, mRNA
sequence
Length = 676
Score = 220 bits (474), Expect = 2e-057
Identities = 88/104 (84%), Positives = 93/104 (89%)
Frame = -1 / +1
Query: 578 VGVVAGISYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRQNRTPTIVVVWAILL 399
VGVVAG SYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGR NRTPTIV+VWAILL
Sbjct: 55 VGVVAGTSYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILL 234
Query: 398 ASIFSLLWVRIDPFTTRVTGPDTRTCGINC*GGGRFVETERYHE 267
ASIFSLLWVRIDPFTTRV GP+ +TCGINC* GR +Y+E
Sbjct: 235 ASIFSLLWVRIDPFTTRVAGPNIQTCGINC*ENGRLQRQRQYNE 366
Score = 199 bits (428), Expect = 4e-051
Identities = 87/119 (73%), Positives = 99/119 (83%)
Frame = +1 / -3
Query: 265 HSWYLSVSTNLPPP*QLMPHVRVSGPVTRVVNGSMRTHSKEKIDASRIAQTTTMVGVRFC 444
+S Y NLP *QL+P V + GP TRVVNGS+RTHSKEKI+A RIAQT T+VGVRF
Sbjct: 368 NSLYCLCLCNLPFS*QLIPQVWMFGPATRVVNGSIRTHSKEKIEARRIAQTMTIVGVRFV 189
Query: 445 RPMRPLRNGYR*TITQKAKKSLPKSGPHDWYPLLMA*EMPATTPTRLMIRMVVGGIRSV 621
P+RPLRNGY+*TITQKAKKS P SGPHDW PLLMA*E+PATTPT LMI++VVGG+RSV
Sbjct: 188 LPIRPLRNGYK*TITQKAKKSFPNSGPHDW*PLLMA*EVPATTPTMLMIKIVVGGMRSV 12
Score = 154 bits (331), Expect = 8e-038
Identities = 62/119 (52%), Positives = 85/119 (71%)
Frame = -3 / +3
Query: 621 DTSDPAHHHPDHQPGRRCCRHLLRHQQRVPVVGSALRQALLRLLGDRSPVPVPQGSHGSA 442
DTS P++++ DH GR CC HLL HQQ +P++G+A+R+ALL LLGD S +P+PQGS+G
Sbjct: 12 DTSHPSNNNFDH*HGRCCCWHLLCHQQWLPIMGAAVRKALLCLLGDCSLIPIPQGSYGQD 191
Query: 441 EPHPDHRGCLGDPAGVDLLLAVGSHRSVHHPRHWPGYSNVWHQLLGRWKVCRNREIPRM 265
+PH D LG P DLLLAVGS+ S+H+P WP + N+W QLLG+W++ I R+
Sbjct: 192 KPHTDDCHRLGYPPRFDLLLAVGSY*SIHYPGCWPEHPNLWDQLLGKWEIAETETIQRI 368
Score = 116 bits (248), Expect = 2e-026
Identities = 52/96 (54%), Positives = 66/96 (68%)
Frame = +3 / -2
Query: 267 FVVSLCFYKPSTSLAVDATRSSIRASDAGGERIDANPQQGEDRRQQDRPDNHDGRGAVLP 446
FVV + LAVD T +RAS+ G E I+ NPQQGEDR ++D PD+ + R AV P
Sbjct: 366 FVVLSLSLQSPIFLAVDPTGLDVRASNPGSEWINTNPQQGEDRSEEDSPDDDNRRCAVCP 187
Query: 447 THETLEERVQVNDHPEGEEELAEERTPRLVPAVDGV 554
H+TLEE V VN+HPEG+EEL+E+R P LV VDG+
Sbjct: 186 AHKTLEEWV*VNNHPEGKEELSEQRPP*LVTTVDGI 79
>gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_H12_O23, mRNA
sequence
Length = 620
Score = 194 bits (418), Expect = 8e-050
Identities = 77/92 (83%), Positives = 81/92 (88%)
Frame = -1 / +2
Query: 542 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRQNRTPTIVVVWAILLASIFSLLWVRID 363
SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGR NRTPTIV+VWAILLASIFSLLWVRID
Sbjct: 2 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILLASIFSLLWVRID 181
Query: 362 PFTTRVTGPDTRTCGINC*GGGRFVETERYHE 267
PFTTRV GP+ +TCGINC* GR +Y E
Sbjct: 182 PFTTRVAGPNIQTCGINC*ENGRLQRQRQYIE 277
Score = 155 bits (332), Expect = 6e-038
Identities = 67/93 (72%), Positives = 75/93 (80%)
Frame = +1 / -3
Query: 265 HSWYLSVSTNLPPP*QLMPHVRVSGPVTRVVNGSMRTHSKEKIDASRIAQTTTMVGVRFC 444
+S Y NLP *QL+P V + GP TRVVNGS+RTHSKEKI+A RIAQT T+VGVRF
Sbjct: 279 NSMYCLCLCNLPFS*QLIPQVWMFGPATRVVNGSIRTHSKEKIEARRIAQTMTIVGVRFV 100
Query: 445 RPMRPLRNGYR*TITQKAKKSLPKSGPHDWYPL 543
P+RPLRNGY+*TITQKAKKS PKSGPHDW PL
Sbjct: 99 LPIRPLRNGYK*TITQKAKKSFPKSGPHDW*PL 1
Score = 129 bits (276), Expect = 3e-030
Identities = 51/93 (54%), Positives = 68/93 (73%)
Frame = -3 / +1
Query: 543 QRVPVVGSALRQALLRLLGDRSPVPVPQGSHGSAEPHPDHRGCLGDPAGVDLLLAVGSHR 364
Q +P++G+ALR+ALL LLGD S +PVPQGS+G +PH D R LG P DLLLAVGS+R
Sbjct: 1 QWLPIMGAALRKALLCLLGDCSLIPVPQGSYGQDKPHTDDRHRLGYPPRFDLLLAVGSYR 180
Query: 363 SVHHPRHWPGYSNVWHQLLGRWKVCRNREIPRM 265
S+H+P WP + N+W QLLG+W++ I R+
Sbjct: 181 SIHYPGRWPEHPNLWDQLLGKWEIAETETIHRI 279
Score = 119 bits (254), Expect = 4e-027
Identities = 51/77 (66%), Positives = 59/77 (76%)
Frame = +3 / -2
Query: 306 LAVDATRSSIRASDAGGERIDANPQQGEDRRQQDRPDNHDGRGAVLPTHETLEERVQVND 485
LAVD T +RASD G E ID NPQQGEDR ++D PD+ D R AV P H+TLEERV VN+
Sbjct: 238 LAVDPTGLDVRASDPGSEWIDTNPQQGEDRSEEDSPDDDDRRCAVCPAHKTLEERV*VNN 59
Query: 486 HPEGEEELAEERTPRLV 536
HPEG+EEL+EER P LV
Sbjct: 58 HPEGKEELSEERPP*LV 8
>gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_G06_M12, mRNA sequence
Length = 521
Score = 182 bits (393), Expect = 2e-046
Identities = 69/91 (75%), Positives = 81/91 (89%)
Frame = -1 / +2
Query: 578 VGVVAGISYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRQNRTPTIVVVWAILL 399
VG+VAG+S A+N+GY SWGPLFGKLFF+FWVIVHLYPFLKGLMGRQNRTPTIVV+W++LL
Sbjct: 62 VGIVAGVSDAVNNGYGSWGPLFGKLFFSFWVIVHLYPFLKGLMGRQNRTPTIVVLWSVLL 241
Query: 398 ASIFSLLWVRIDPFTTRVTGPDTRTCGINC* 306
ASIFSL+WVRIDPF + GP + CG+ C*
Sbjct: 242 ASIFSLVWVRIDPFIAKSKGPILKPCGVQC* 334
Score = 140 bits (301), Expect(2) = 2e-034
Identities = 63/103 (61%), Positives = 73/103 (70%)
Frame = +1 / -3
Query: 319 PHVRVSGPVTRVVNGSMRTHSKEKIDASRIAQTTTMVGVRFCRPMRPLRNGYR*TITQKA 498
PH GP+ + GS+ T ++EK++ASR Q+TTMVGVRFC PM PLRNG R T+TQK
Sbjct: 321 PHGLRMGPLDLAMKGSILTQTREKMEASRTDQSTTMVGVRFCLPMSPLRNG*RWTMTQKE 142
Query: 499 KKSLPKSGPHDWYPLLMA*EMPATTPTRLMIRMVVGGIRSVVH 627
KKS PK GPHD PLL A E PAT PT LM+ VVGGI VVH
Sbjct: 141 KKSFPKRGPHDP*PLLTASETPATMPTMLMMMSVVGGISRVVH 13
Score = 100 bits (214), Expect = 1e-021
Identities = 44/91 (48%), Positives = 57/91 (62%)
Frame = -3 / +1
Query: 627 VDDTSDPAHHHPDHQPGRRCCRHLLRHQQRVPVVGSALRQALLRLLGDRSPVPVPQGSHG 448
VD +DP ++ HQ GR CC R QQ + V+G LR+ALL LLG R P+P+PQG+HG
Sbjct: 13 VDHPTDPTNYTHHHQHGRHCCWCFRRSQQWLRVMGPPLREALLFLLGHRPPLPIPQGAHG 192
Query: 447 SAEPHPDHRGCLGDPAGVDLLLAVGSHRSVH 355
A+PH HR L PA + LL +G S+H
Sbjct: 193 EAKPHAHHRRALVCPARLHLLPRLGQD*SLH 285
Score = 22.1 bits (42), Expect(2) = 2e-034
Identities = 8/23 (34%), Positives = 15/23 (65%)
Frame = +1 / -3
Query: 238 TNRQFVAAAHSWYLSVSTNLPPP 306
TN QF++ + + + S+N+ PP
Sbjct: 441 TN*QFISLQNLYRTNNSSNMKPP 373
>gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_A01_A02, mRNA sequence
Length = 474
Score = 115 bits (246), Expect = 5e-026
Identities = 43/60 (71%), Positives = 51/60 (85%)
Frame = -1 / +3
Query: 488 VIVHLYPFLKGLMGRQNRTPTIVVVWAILLASIFSLLWVRIDPFTTRVTGPDTRTCGINC 309
VIVHLYPFLKGL+GRQNRTPTIV+VW+ILLASI SLLWVR++PF + GP CG++C
Sbjct: 12 VIVHLYPFLKGLLGRQNRTPTIVIVWSILLASIISLLWVRVNPFLAKTDGPTLEECGLDC 191
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 43,138,499
Number of Sequences: 8143
Number of extensions: 683743
Number of successful extensions: 3425
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 47
effective length of database: 1,184,766
effective search space used: 1437121158
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)