TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441110.2.1
(643 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815005.1|CN815005 HRO4504_G12_N24ZS5 Lib AA071E1X Aven... 201 2e-058
gb|AF084005.1|AF084005 Avena fatua ras-like small monomeric... 196 3e-051
>gb|CN815005.1|CN815005 HRO4504_G12_N24ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4504_G12_N24, mRNA sequence
Length = 633
Score = 201 bits (434), Expect(4) = 2e-058
Identities = 85/97 (87%), Positives = 86/97 (88%)
Frame = -1 / +3
Query: 577 ERFAESKKEXXXXXXXXXXANVPFLILGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNL 398
ERFAESKKE ANVPFLILGNKIDIPYAASEEELRYHLGLSNFTTGKGKV+L
Sbjct: 72 ERFAESKKELDALLSDDSLANVPFLILGNKIDIPYAASEEELRYHLGLSNFTTGKGKVSL 251
Query: 397 GDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIK*GPC 287
GDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIK*G C
Sbjct: 252 GDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIK*GSC 362
Score = 196 bits (423), Expect(3) = 7e-055
Identities = 84/113 (74%), Positives = 85/113 (75%)
Frame = +1 / -1
Query: 289 KGPT*CIGRPT*NHRRSPSCEQHCT*KPQVAGHWSRQG*PCPSRL*SC*GLGDTAAPLQR 468
K PT*C GRPT* H SPSC QHCT* PQ AGHWS QG*PCPS+L*SC*GL DTAA LQR
Sbjct: 360 KSPT*CTGRPT*THHHSPSCGQHCT*TPQEAGHWSHQG*PCPSQL*SC*GLSDTAALLQR 181
Query: 469 QHTGYQSCCQG*EMEHLPRNHLTGERPAPFLIQQIAPYHTHLPGILQHQPLRS 627
QH GYQS CQ *EM LP NH GE APF IQQ AP HLP ILQHQP RS
Sbjct: 180 QHMGYQSYCQE*EMGRLPGNHQKGEHRAPFSIQQSAPCRMHLPSILQHQP*RS 22
Score = 193 bits (417), Expect(2) = 4e-052
Identities = 84/114 (73%), Positives = 91/114 (79%)
Frame = -3 / +2
Query: 629 LLRKG*CCSIPGRCV**GAIC*IKKGAGRSPVR*FLGKCSISHPWQQD*YPVCCL*RGAA 450
+LR G*CCSI GRC+ GA+C*I+KGA SP *F GK ISH WQ D*YP+CCL*R AA
Sbjct: 20 ILR*G*CCSILGRCIRQGALC*IEKGARCSPF**FPGKRPISHSWQ*D*YPICCL*RRAA 199
Query: 449 VSPRP*QLHNREGQGQPWRLQCPAT*GFHVQCCSQDGLRRWFQVGLPIHQVGPL 288
VS RP*QLHN EGQGQPW LQCPA+*G HVQCC QDGL W QVGLP+HQVG L
Sbjct: 200 VSLRP*QLHNWEGQGQPW*LQCPAS*GVHVQCCPQDGLW*WVQVGLPVHQVGLL 361
Score = 188 bits (406), Expect(2) = 2e-050
Identities = 81/115 (70%), Positives = 91/115 (79%)
Frame = +2 / -2
Query: 284 PARALLDVLGDPLETIAVAHLANNTAHENLKWPDIGVAKVDLALPGCEVAKA*VIPQLLF 463
P RALLDVLGDPLE I +AHLA+NTAHE+LK PDIGV K DLALP CEVAKA*VIPQL F
Sbjct: 365 PTRALLDVLGDPLEPITIAHLADNTAHEHLKRPDIGVTKADLALPSCEVAKA*VIPQLFF 186
Query: 464 RGSIRDINLVAKDEKWNICQGII*QESVQXXXXXXXXXXXXRIYQVYYSINLCVV 628
RGSI DINL+AK+EKW++CQGII +ES++ IYQVYYSINL VV
Sbjct: 185 RGSIWDINLIAKNEKWDVCQGIIRKESIELLFRFSKALLVVCIYQVYYSINLSVV 21
Score = 187 bits (403), Expect(2) = 1e-049
Identities = 88/115 (76%), Positives = 91/115 (79%)
Frame = -2 / +1
Query: 627 TTQRLML*YTW*MRMIRSDLLNQKRSWTLSCQMIPWQMFHFSSLATRLISRMLPLKRSCG 448
TT RLML*YTW*M RS LLN+KRS LS MIPWQ HFS LA RLIS MLPLK+SCG
Sbjct: 22 TTLRLML*YTW*MHTTRSALLNRKRSSMLSFLMIPWQTSHFSFLAIRLISHMLPLKKSCG 201
Query: 447 IT*ALATSQPGRARSTLATPMSGHLRFSCAVLFARWATAMVSSGSPNTSSRALAG 283
IT*ALATSQ GRARS L TPMSG LR SCAVL ARWA M SSGSP+TSSRAL G
Sbjct: 202 IT*ALATSQLGRARSALVTPMSGLLRCSCAVLSARWAMVMGSSGSPSTSSRALVG 366
Score = 170 bits (365), Expect = 3e-043
Identities = 70/81 (86%), Positives = 73/81 (90%)
Frame = +3 / -3
Query: 285 RQGPYLMYWETHLKPSP*PILRTTLHMKTSSGRTLESPRLTLPFPVVKLLRPR*YRXXXX 464
+Q PYLMYWETHL PSP*PILRTTLHM TS GRTLESPRLTLPFPVVKLLRP+*YR
Sbjct: 364 QQEPYLMYWETHLNPSP*PILRTTLHMNTSRGRTLESPRLTLPFPVVKLLRPK*YRSSSS 185
Query: 465 EAAYGISILLPRMRNGTFAKE 527
EAAYGISILLPRMRNGTFA+E
Sbjct: 184 EAAYGISILLPRMRNGTFARE 122
Score = 27.6 bits (54), Expect(3) = 7e-055
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +1 / -1
Query: 136 FNMFPNVKHCFFLFIP 183
F+M PNVK CF L P
Sbjct: 507 FSMVPNVKRCFSLSYP 460
Score = 27.2 bits (53), Expect(4) = 2e-058
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = -3 / +2
Query: 182 GMKRKKQCLTLGNMLN 135
G +R+KQ LTLG MLN
Sbjct: 461 GYEREKQRLTLGTMLN 508
Score = 25.8 bits (50), Expect(2) = 4e-052
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = -2 / +1
Query: 174 EEKTVLNIGEHVESL 130
+ KT LNIG H ESL
Sbjct: 469 KRKTTLNIGYHAESL 513
Score = 24.9 bits (48), Expect(4) = 2e-058
Identities = 8/12 (66%), Positives = 11/12 (91%)
Frame = -2 / +2
Query: 105 VKLLEFFCFSVG 70
V+LL+ FCFS+G
Sbjct: 536 VELLKLFCFSIG 571
Score = 24.9 bits (48), Expect(2) = 2e-050
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = +3 / -3
Query: 135 IQHVPQC*ALFF 170
IQH QC*ALFF
Sbjct: 508 IQHGTQC*ALFF 473
Score = 24.0 bits (46), Expect(2) = 1e-049
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -1 / +3
Query: 169 KNSA*HWGTC*I 134
KN+A*HW C*I
Sbjct: 474 KNNA*HWVPC*I 509
Score = 23.5 bits (45), Expect(4) = 2e-058
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -1 / +1
Query: 259 FCLVICSGWYFVT*TMI 209
F V C GW F+T MI
Sbjct: 394 FFPVSCFGWLFITTAMI 444
Score = 23.1 bits (44), Expect(3) = 7e-055
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1 / -3
Query: 67 RSYGKAKEFQEFD*HN 114
+ YGKAK+F E D N
Sbjct: 574 KPYGKAKQF*ELDISN 527
>gb|AF084005.1|AF084005 Avena fatua ras-like small monomeric GTP-binding protein (SAR1)
mRNA, complete cds
Length = 801
Score = 196 bits (422), Expect = 3e-051
Identities = 84/117 (71%), Positives = 87/117 (74%)
Frame = +1 / -2
Query: 289 KGPT*CIGRPT*NHRRSPSCEQHCT*KPQVAGHWSRQG*PCPSRL*SC*GLGDTAAPLQR 468
+ P *C GRPT* H SPSC QHCT* PQ AGHWS +G*PCPS+L*SC*GL DTAA LQR
Sbjct: 752 ESPN*CTGRPT*THHHSPSCGQHCT*TPQEAGHWSHKG*PCPSQL*SC*GLSDTAALLQR 573
Query: 469 QHTGYQSCCQG*EMEHLPRNHLTGERPAPFLIQQIAPYHTHLPGILQHQPLRSNPSR 639
QH GYQS CQ *EM LP NH GE APF IQQ AP HLP ILQHQP RSN SR
Sbjct: 572 QHMGYQSYCQE*EMGRLPGNHQKGEHRAPFSIQQSAPCRMHLPSILQHQP*RSNLSR 402
Score = 194 bits (418), Expect = 1e-050
Identities = 82/120 (68%), Positives = 94/120 (78%)
Frame = +2 / -3
Query: 284 PARALLDVLGDPLETIAVAHLANNTAHENLKWPDIGVAKVDLALPGCEVAKA*VIPQLLF 463
P RAL+DVLGDPLE I +AHLA+NTAHE+LK PDIGV K DLALP CE AKA*VIPQL F
Sbjct: 757 PTRALIDVLGDPLEPITIAHLADNTAHEHLKRPDIGVTKADLALPSCEAAKA*VIPQLFF 578
Query: 464 RGSIRDINLVAKDEKWNICQGII*QESVQXXXXXXXXXXXXRIYQVYYSINLCVVILPDA 643
RGSI DINL+AK+EKW++CQGII +ES++ IYQVYYSINL VVI PD+
Sbjct: 577 RGSIWDINLIAKNEKWDVCQGIIRKESIELLFRFSKALLVVCIYQVYYSINLSVVIFPDS 398
Score = 190 bits (409), Expect = 2e-049
Identities = 84/115 (73%), Positives = 91/115 (79%)
Frame = -3 / +1
Query: 638 LEGLLRKG*CCSIPGRCV**GAIC*IKKGAGRSPVR*FLGKCSISHPWQQD*YPVCCL*R 459
LE LLR G*CCSI GRC+ GA+C*I+KGA SP *F GK ISH WQ D*YP+CCL*R
Sbjct: 403 LERLLR*G*CCSILGRCIRQGALC*IEKGARCSPF**FPGKRPISHSWQ*D*YPICCL*R 582
Query: 458 GAAVSPRP*QLHNREGQGQPWRLQCPAT*GFHVQCCSQDGLRRWFQVGLPIHQVG 294
AAVS RP*QLHN EGQGQP LQCPA+*G HVQCC QDGL W QVGLP+HQ+G
Sbjct: 583 RAAVSLRP*QLHNWEGQGQPL*LQCPAS*GVHVQCCPQDGLW*WVQVGLPVHQLG 747
Score = 189 bits (407), Expect = 4e-049
Identities = 81/95 (85%), Positives = 83/95 (87%)
Frame = -1 / +2
Query: 577 ERFAESKKEXXXXXXXXXXANVPFLILGNKIDIPYAASEEELRYHLGLSNFTTGKGKVNL 398
ERFAESKKE ANVPFLILGNKIDIPYAASEEELRYHLGLS+FTTGKGKV+L
Sbjct: 464 ERFAESKKELDALLSDDSLANVPFLILGNKIDIPYAASEEELRYHLGLSSFTTGKGKVSL 643
Query: 397 GDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIK*G 293
DSNVRPLEVFMCSVVRKMGYGDGFKWVSQYI *G
Sbjct: 644 CDSNVRPLEVFMCSVVRKMGYGDGFKWVSQYIN*G 748
Score = 188 bits (404), Expect = 1e-048
Identities = 88/119 (73%), Positives = 92/119 (77%)
Frame = -2 / +3
Query: 639 SGRITTQRLML*YTW*MRMIRSDLLNQKRSWTLSCQMIPWQMFHFSSLATRLISRMLPLK 460
SG+ITT RLML*YTW*M RS LLN+KRS LS MIPWQ HFS LA RLIS MLPLK
Sbjct: 402 SGKITTLRLML*YTW*MHTTRSALLNRKRSSMLSFLMIPWQTSHFSFLAIRLISHMLPLK 581
Query: 459 RSCGIT*ALATSQPGRARSTLATPMSGHLRFSCAVLFARWATAMVSSGSPNTSSRALAG 283
+SCGIT*ALA SQ GRARS TPMSG LR SCAVL ARWA M SSGSP+TS RAL G
Sbjct: 582 KSCGIT*ALAASQLGRARSAFVTPMSGLLRCSCAVLSARWAMVMGSSGSPSTSIRALVG 758
Score = 159 bits (342), Expect = 4e-040
Identities = 67/78 (85%), Positives = 69/78 (88%)
Frame = +3 / -1
Query: 294 PYLMYWETHLKPSP*PILRTTLHMKTSSGRTLESPRLTLPFPVVKLLRPR*YRXXXXEAA 473
P LMYWETHL PSP*PILRTTLHM TS GRTLES RLTLPFPVVKLLRP+*YR EAA
Sbjct: 747 P*LMYWETHLNPSP*PILRTTLHMNTSRGRTLESQRLTLPFPVVKLLRPK*YRSSSSEAA 568
Query: 474 YGISILLPRMRNGTFAKE 527
YGISILLPRMRNGTFA+E
Sbjct: 567 YGISILLPRMRNGTFARE 514
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,120,130
Number of Sequences: 8143
Number of extensions: 108842
Number of successful extensions: 1270
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 209557251
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)