TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441100.2.1
(646 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819880.1|CN819880 HRO4407_C04_F07ZS5 Lib AA069E1X Aven... 153 3e-038
gb|CN814897.1|CN814897 HRO4503_F11_L20ZS5 Lib AA071E1X Aven... 135 6e-033
>gb|CN819880.1|CN819880 HRO4407_C04_F07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C04_F07, mRNA
sequence
Length = 741
Score = 153 bits (328), Expect = 3e-038
Identities = 63/90 (70%), Positives = 69/90 (76%)
Frame = +3 / +1
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
VEACK ATGA+IKVEYL RRPGDYAEVYS+PSKI ELNWTAQ TDL +SLA AWKWQK
Sbjct: 442 VEACKAATGATIKVEYLTRRPGDYAEVYSNPSKIRDELNWTAQRTDLRESLATAWKWQKT 621
Query: 189 HPNGYGPA*FRVQRLRETRHADPKGPCAVM 278
HP GYG A* + R R R + PCAV+
Sbjct: 622 HPGGYGSA*LLLSRFRFQRSCPTQDPCAVI 711
Score = 127 bits (272), Expect = 2e-030
Identities = 61/92 (66%), Positives = 65/92 (70%)
Frame = -1 / -2
Query: 277 MTAHGPFGSA*RVSLNL*TLNQAGPYPFGCAFCHFHAWARLWPRSVYWAVQLSSMWIFDG 98
MTAHG R +LNL + NQA PYP GC FCHFHA ARL RSV WAVQ SS I DG
Sbjct: 710 MTAHGSCVGQLR*NLNLLSSNQADPYPPGCVFCHFHAVARLSRRSVRWAVQFSSSRILDG 531
Query: 97 SLYTSA*SPGLRARYSTLIEAPVAFLQASTRA 2
L TSA*SPGLR RYSTL+ APVA L AST +
Sbjct: 530 LL*TSA*SPGLRVRYSTLMVAPVAALHASTNS 435
Score = 100 bits (212), Expect = 3e-022
Identities = 43/93 (46%), Positives = 59/93 (63%)
Frame = +2 / +3
Query: 2 GTSRSLQEGNRSFYQGRISCT*TRRLCRGIQRSVENPHGTQLDSPVHRSWPKPRPGVEMA 181
G RS+Q G+RS++QGRI TRRLC +Q+ V++P +LDSP H S KPR G+EMA
Sbjct: 435 GVCRSVQGGDRSYHQGRIPYPETRRLC*SLQQPVQDPRRAELDSPTHGSSRKPRDGMEMA 614
Query: 182 ESAPERIWTSLIQSSKIERDSSRRSKGSMCSHV 280
E P I LI + ++ + + GSMCSH+
Sbjct: 615 EDTPRGIRIGLITAQ*VQISAKLSNTGSMCSHI 713
>gb|CN814897.1|CN814897 HRO4503_F11_L20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_F11_L20, mRNA sequence
Length = 610
Score = 135 bits (290), Expect = 6e-033
Identities = 50/66 (75%), Positives = 60/66 (90%)
Frame = +3 / +1
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
V+ACKKATG IKVEYL+RRPGDYAEV+SDP+KI+ ELNWTAQ+TDL +SL+ AW+WQK+
Sbjct: 91 VDACKKATGVDIKVEYLSRRPGDYAEVFSDPTKINSELNWTAQHTDLKESLSVAWRWQKS 270
Query: 189 HPNGYG 206
HP GYG
Sbjct: 271 HPRGYG 288
Score = 97.7 bits (207), Expect = 2e-021
Identities = 47/68 (69%), Positives = 50/68 (73%)
Frame = -1 / -3
Query: 205 PYPFGCAFCHFHAWARLWPRSVYWAVQLSSMWIFDGSLYTSA*SPGLRARYSTLIEAPVA 26
P P GC FCH HA RL RSV AVQLSS+ IF GSL TSA*SPGL RYSTL+ PVA
Sbjct: 287 P*PRGCDFCHLHATDRLSLRSVC*AVQLSSLLIFVGSLNTSA*SPGLLLRYSTLMSTPVA 108
Query: 25 FLQASTRA 2
FLQAST +
Sbjct: 107 FLQASTNS 84
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,295,539
Number of Sequences: 8143
Number of extensions: 108198
Number of successful extensions: 1260
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 210782732
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)