TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441058.2.1
(1000 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN820454.1|CN820454 HRO4419_E09_J17ZS5 Lib AA069E1X Aven... 308 2e-085
>gb|CN820454.1|CN820454 HRO4419_E09_J17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4419_E09_J17, mRNA
sequence
Length = 739
Score = 308 bits (668), Expect(2) = 2e-085
Identities = 131/150 (87%), Positives = 138/150 (92%)
Frame = +1 / +3
Query: 298 SLRCSGLYLSQFRHTSPHLLASGDGKRTATIVGDVYIHPSAKVHPTSKIGPNVSISANAR 477
SLR SGLYLSQFRHTSPHLLASGDGK+ ATI+GDVYIHPSAKVHPT+KIGPNVS+SANAR
Sbjct: 6 SLRFSGLYLSQFRHTSPHLLASGDGKKNATIIGDVYIHPSAKVHPTAKIGPNVSLSANAR 185
Query: 478 VGAGARLINCIILDDVEIMENAVVIHSIVGWKSSIGKWSRVQGEGDHNAKLGITILGEAX 657
+GAGARLINCIILDDVEIM+NAVVIHSIVGWKS+IGKWSRVQGEGDHNAKLGITILGEA
Sbjct: 186 IGAGARLINCIILDDVEIMDNAVVIHSIVGWKSTIGKWSRVQGEGDHNAKLGITILGEAV 365
Query: 658 XXXXXXXXXNSIVLPNKTLNVSVQEEIIL* 747
NSIVLPNKTLNVSVQEEIIL*
Sbjct: 366 DVEDEVVVTNSIVLPNKTLNVSVQEEIIL* 455
Score = 230 bits (497), Expect(3) = 1e-067
Identities = 99/151 (65%), Positives = 106/151 (70%)
Frame = -3 / -1
Query: 749 YYRMISSWTLTLRVLFGXXXXXXXXXXXXXXXXSPRIVMPSLAL*SPSPCTRDHFPMDDF 570
YYRMISS TLTLRVLFG SPR+V+PSLAL SPSPCTRDHFP+ DF
Sbjct: 457 YYRMISSCTLTLRVLFGSTILLVTTTSSSTSTASPRMVIPSLALWSPSPCTRDHFPIVDF 278
Query: 569 HPTIECXXXXXXXXXXXXXIMQLISLAPAPTRAFADIETFGPIFEVGCTLADGWI*TSPT 390
PTIEC ++QLISLAPAP RAFAD ET GPIF VGCT ADGWI TSP
Sbjct: 277 QPTIECITTALSIISTSSRMIQLISLAPAPMRAFADNETLGPIFAVGCTFADGWIYTSPI 98
Query: 389 IVAVLLPSPEAKR*GEVWRN*ERYNPEHLKD 297
IVA LPSPEAKR*G+VWRN ERY PE+ KD
Sbjct: 97 IVAFFLPSPEAKR*GDVWRNWERYRPENRKD 5
Score = 194 bits (419), Expect(2) = 3e-054
Identities = 90/142 (63%), Positives = 102/142 (71%)
Frame = -2 / -3
Query: 723 TDIESFIWEHDAVXXXXXXXXXNGFTKNSNAKFGIVITFALYT*PFSNG*FPPYN*MYNN 544
TDIESF+WEH+ + NGFTKN + KFGIV+TF LYT*PFS * P + *MYN+
Sbjct: 431 TDIESFVWEHNTISYYYFIFNINGFTKNGDTKFGIVVTFTLYT*PFSYC*LPTHY*MYND 252
Query: 543 SIFHNFNIIQNYAIDKPGTSSNACIC*YRDIWANL*SRMHLGRWMDIDIANNSGSSFAIS 364
I HNFNII N IDKPGT SNACIC* RD+ A+L S MHL RWMDI I NSG FA+S
Sbjct: 251 CIIHNFNII*NDTIDKPGTCSNACIC**RDVGADLCSWMHLCRWMDIHITYNSGIFFAVS 72
Query: 363 *G*KMRRGMAKLREI*PRASQR 298
*KMRR MA+L +I* R SQR
Sbjct: 71 RS*KMRRCMAELGKI*TRESQR 6
Score = 121 bits (258), Expect(2) = 1e-033
Identities = 64/108 (59%), Positives = 68/108 (62%)
Frame = +2 / +1
Query: 305 DALGYISLSFAIPLLIF*PQEMAKELPLLLAMSISIHLPRCILLQRLAQMSLYQQMHALE 484
D+L YI S AI LLIF* E AK++PLL M ISIHL RCI LQR A S YQQMHALE
Sbjct: 13 DSLVYIFPSSAIHLLIF*LLETAKKMPLL*VMCISIHLQRCIQLQRSAPTSRYQQMHALE 192
Query: 485 LVPGLSIA*FXXXXXXXXXXXXYIQL*GGNHPLENGHVYRAKVITMPN 628
VPGLSI F YIQ G+ ENGHVYR KV TMPN
Sbjct: 193 QVPGLSIVSF*MMLKLWIMQSLYIQ*WVGSQQ*ENGHVYRVKVTTMPN 336
Score = 106 bits (227), Expect(2) = 1e-044
Identities = 46/68 (67%), Positives = 55/68 (80%)
Frame = +3 / +2
Query: 552 TFNCRVEIIHWKMVTCTGRR*SQCQTWHYYSW*SR*C*R*SSCS*QHRAPK*NSQCQCPR 731
TFN +E+ + KMVTCTG R* QCQTW+++SW*SR*C*R*SS +* + APK NSQCQC R
Sbjct: 260 TFNSGLEVNNRKMVTCTG*R*PQCQTWYHHSW*SR*C*R*SSSN**YCAPKQNSQCQCAR 439
Query: 732 GDHPIITI 755
DHPII +
Sbjct: 440 RDHPIILL 463
Score = 103 bits (220), Expect(3) = 1e-034
Identities = 46/70 (65%), Positives = 51/70 (72%)
Frame = -1 / -2
Query: 505 N**AWHQLQRVHLLI*RHLGQSLK*DAPWQMDGYRHRQQ*WQFFCHLLRLKDEERYGETE 326
N**AWH LQ VHLLI R G+SL+ DAP QMDGY H *W FFC L +LKDEE YG T
Sbjct: 213 N**AWHLLQCVHLLITRRWGRSLQLDAPLQMDGYTHHL**WHFFCRLQKLKDEEMYGGTG 34
Query: 325 RDITQSISKT 296
+DI Q I+KT
Sbjct: 33 KDIDQRIAKT 4
Score = 88.6 bits (187), Expect(2) = 1e-044
Identities = 38/73 (52%), Positives = 44/73 (60%)
Frame = +3 / +2
Query: 297 VFEMLWVIXXXXXXXXXXXXXXRRWQKNCHYCWRCLYPSICQGASYFKDWPKCLYISKCT 476
VF +LW I R QK CHY C+YPSIC+GAS KD P+ L ISKCT
Sbjct: 5 VFAILWSISFPVPPYISSSFSFWRRQKKCHYYR*CVYPSICKGASNCKDRPQRLVISKCT 184
Query: 477 RWSWCQAYQLHNS 515
WS CQAYQL++S
Sbjct: 185 HWSRCQAYQLYHS 223
Score = 44.6 bits (91), Expect(3) = 1e-034
Identities = 17/26 (65%), Positives = 18/26 (69%)
Frame = -1 / -2
Query: 628 VWHCDHLRPVHVTIFQWMISTLQLNV 551
VWHC HL PVHVTIF + S LNV
Sbjct: 336 VWHCGHLHPVHVTIFLLLTSNPLLNV 259
Score = 37.3 bits (75), Expect(2) = 1e-033
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = +2 / +1
Query: 695 CSQIKLSMSVSKRRSSYN 748
CSQ KLSMSV K+RSSYN
Sbjct: 403 CSQTKLSMSVCKKRSSYN 456
Score = 35.4 bits (71), Expect(3) = 1e-067
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = -1 / -1
Query: 859 TNNLDLMEGYITW*ERINLNYVIGTQTLYGI 767
TN+L+LM YI E+ NY+ G +TL+GI
Sbjct: 577 TNDLNLMTAYIGEPEKKKGNYIFGMKTLFGI 485
Score = 32.7 bits (65), Expect(2) = 3e-054
Identities = 16/35 (45%), Positives = 21/35 (60%)
Frame = -3 / -3
Query: 878 KTGINAN**SRSNGRLHYMVRTNKLKLCDWYSDLI 774
K ++ +**S+SN LH R K KL WY +LI
Sbjct: 596 KLRVSID**SQSNDSLHRGTREEKRKLHIWYENLI 492
Score = 31.3 bits (62), Expect(3) = 1e-034
Identities = 15/19 (78%), Positives = 16/19 (84%)
Frame = -1 / -2
Query: 748 IIG*SPLGH*H*EFYLGAR 692
IIG*S L H*H*EF LGA+
Sbjct: 456 IIG*SLLAH*H*EFCLGAQ 400
Score = 24.9 bits (48), Expect(3) = 1e-067
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = -3 / -2
Query: 977 LTKNGVTCLYIFYI 936
L KN V CL+ FYI
Sbjct: 696 LNKNAVICLHSFYI 655
Score = 22.6 bits (43), Expect(2) = 2e-085
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +2 / +2
Query: 830 VTFH*IEIISLHL 868
V+ H*IEIISL+L
Sbjct: 548 VSCH*IEIISLYL 586
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,416,032
Number of Sequences: 8143
Number of extensions: 161021
Number of successful extensions: 755
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 348248160
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)