TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440773.2.1
(1437 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816396.1|CN816396 HRO4520_C05_F10ZS5 Lib AA071E1X Aven... 272 1e-082
gb|CN814888.1|CN814888 HRO4503_E12_J23ZS5 Lib AA071E1X Aven... 244 7e-066
gb|CN816242.1|CN816242 HRO4518_E06_I12ZS5 Lib AA071E1X Aven... 121 2e-037
>gb|CN816396.1|CN816396 HRO4520_C05_F10ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_C05_F10, mRNA sequence
Length = 851
Score = 272 bits (589), Expect(2) = 1e-082
Identities = 92/159 (57%), Positives = 120/159 (75%)
Frame = +2 / +2
Query: 275 RHTICRQDVEKVVCLLCETKQPVSQVCISCGVNMGEYFCDICKFYDDDTDKGQYHCIDCG 454
RH + R +V++V+C LC T+Q V ++CISCGV MG+YFC++CK +DDD K QYHC CG
Sbjct: 203 RHELSRHEVQQVICSLCGTEQEVGKICISCGVCMGKYFCEVCKLFDDDISKQQYHCHGCG 382
Query: 455 ICRVGGKENFFHCVKCGSCYSVILRDNHQCVENSMRQNCPICYEYLFDSLQGTRVLNCGH 634
ICR+GG ENFFHC KCG CYS +L+++H CVE +M +CPIC+EYLFDS+ G VL CGH
Sbjct: 383 ICRIGGGENFFHCSKCGCCYSTVLKNSHACVEGAMHHDCPICFEYLFDSIDGVSVLRCGH 562
Query: 635 TMHLTCFEEMVAHNKYTCPICSKTALDLTHHWEMLDQEI 751
T+H C +EM H K CP+CSK+ D++ WE LD+EI
Sbjct: 563 TIHENCLKEMEKHRKLICPLCSKSVCDMSRAWERLDREI 679
Score = 141 bits (302), Expect(2) = 5e-042
Identities = 73/142 (51%), Positives = 84/142 (59%)
Frame = -1 / -3
Query: 753 SIS*SNISQ*CVKSRAVLEHIGQVYLLCATISSKHVKCIVCPQFRTLVPCNESNRYS*QI 574
+IS S++S S LE G + L C +IS K IV P +T P ESN+YS QI
Sbjct: 681 AISLSSLSHALDMSHTDLEQSGHINLRCFSISFKQFS*IV*PHRKTDTPSIESNKYSKQI 502
Query: 573 GQFCLIEFSTH*WLSRSITE*QDPHFTQWKKFSLPPTLHMPQSMQWYCPLSVSSS*NLHI 394
GQ I STH WL + E*Q PHF Q KKFS PP LH+PQ Q YC +SSS +LH
Sbjct: 501 GQS*CIAPSTHAWLFFNTVE*QHPHFEQ*KKFSPPPILHIPQP*Q*YCCFEISSSKSLHT 322
Query: 393 SQKYSPILTPQLMHTCDTGCFV 328
SQKY PI TPQLMH T C V
Sbjct: 321 SQKYFPIHTPQLMHIFPTSCSV 256
Score = 87.7 bits (185), Expect(3) = 7e-036
Identities = 30/70 (42%), Positives = 46/70 (65%)
Frame = +2 / +2
Query: 98 DNDVAEVDAARDGEVDRRDVGKMKHGCEHYRRRCKIVAPCCNQVFPCRHCHNEATASGDR 277
+ D A ++ + + +R + G M++GC HYRRRC+I APCCN++F CRHCHNE+ S
Sbjct: 11 EKDSAGINGSSPDDYERLEKGLMQYGCAHYRRRCRIRAPCCNEIFDCRHCHNESKNSIKT 190
Query: 278 HTICRQDVEK 307
I R ++ +
Sbjct: 191 DVIRRHELSR 220
Score = 73.0 bits (153), Expect(3) = 7e-036
Identities = 32/71 (45%), Positives = 45/71 (63%)
Frame = +1 / +1
Query: 334 TAGVTSVHKLWSQYGRVLL*YMQIL***YRQRAVPLHRLWHMQGWWQGKLLPLCEVWVLL 513
T G ++H+LW YG+V L* +Q L***Y + AV L LW+M WW+ + L L ++W+LL
Sbjct: 262 TRGRENMHQLWCMYGKVFL*SVQTL***YLKTAVSLSWLWNM*NWWRREFLSLLKMWMLL 441
Query: 514 FCYTP**PSVC 546
F P +C
Sbjct: 442 FYSVEEQPCMC 474
Score = 50.6 bits (104), Expect(2) = 1e-082
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = +2 / +2
Query: 794 VLCNDCNKVSEVNFHVIGHKCSHCRSYNTR 883
+LCN+C ++EV F++I KC C+SYNTR
Sbjct: 719 ILCNECEAIAEVRFNLIALKCQSCQSYNTR 808
Score = 46.0 bits (94), Expect(2) = 5e-042
Identities = 18/28 (64%), Positives = 20/28 (71%)
Frame = -1 / -3
Query: 255 SLWQWRQGNTWLQHGATILHLRR*CSHP 172
SLWQ RQ LQHGA + HL R*C+HP
Sbjct: 168 SLWQCRQSKISLQHGARMRHLLR*CAHP 85
Score = 24.0 bits (46), Expect(3) = 7e-036
Identities = 11/39 (28%), Positives = 21/39 (53%)
Frame = +1 / +1
Query: 625 LWTHNALDMF*RNGGA*QIHLSNML*NSS*FDTSLGNVG 741
+W++N+ + RNG Q+++ +L S * +G G
Sbjct: 553 VWSYNS*KLLERNGETSQVNMPALLQVSM*HVKGMGETG 669
>gb|CN814888.1|CN814888 HRO4503_E12_J23ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_E12_J23, mRNA sequence
Length = 751
Score = 244 bits (527), Expect(2) = 7e-066
Identities = 91/107 (85%), Positives = 99/107 (92%)
Frame = +2 / +1
Query: 581 YEYLFDSLQGTRVLNCGHTMHLTCFEEMVAHNKYTCPICSKTALDLTHHWEMLDQEIEAT 760
+E LFDSL+GTRVL CGHTMH+ CF EMV HNKY+CPICSKTALD+THHW MLDQEIEAT
Sbjct: 4 HEDLFDSLKGTRVLTCGHTMHMDCFSEMVEHNKYSCPICSKTALDMTHHWGMLDQEIEAT 183
Query: 761 IMPLVYRYKIWVLCNDCNKVSEVNFHVIGHKCSHCRSYNTRTTSRPA 901
IMP VYRYK+WVLCNDCNKVSEV+FHVIGHKCSHC SYNTR+TSRPA
Sbjct: 184 IMPPVYRYKVWVLCNDCNKVSEVDFHVIGHKCSHCSSYNTRSTSRPA 324
Score = 180 bits (388), Expect = 4e-046
Identities = 81/116 (69%), Positives = 88/116 (75%)
Frame = -1 / -3
Query: 930 VEGELLPDKSAGRDVVRVLYDLQWLHLWPIT*KFXXXXXXXXXXXXQIL*RYTRGMIVAS 751
V+GELLPDKSAGR+V RVLY+LQWLHLWPIT K Q L*RYT G+IVAS
Sbjct: 353 VDGELLPDKSAGREVDRVLYELQWLHLWPITWKSTSETLLQSLQRTQTL*RYTGGIIVAS 174
Query: 750 IS*SNISQ*CVKSRAVLEHIGQVYLLCATISSKHVKCIVCPQFRTLVPCNESNRYS 583
IS*SN+ Q*CV SRAV EHIGQ YLLC+TIS K C+VCPQ RTLVP NESNR S
Sbjct: 173 IS*SNMPQ*CVMSRAVFEHIGQEYLLCSTISEKQSMCMVCPQVRTLVPFNESNRSS 6
Score = 162 bits (349), Expect = 1e-040
Identities = 74/117 (63%), Positives = 87/117 (74%)
Frame = -3 / -2
Query: 931 C*R*AASG*ICRARCCSGVVRSAVAALVANHVKVHL*DLVAIVAKHPNLVAIHKRHDRGF 752
C R AS *ICR R S VV +AVAA VANHV+VHL D VA+VAK+PNLV +H+RH+ F
Sbjct: 354 CRRRTAS**ICRPRGRSSVV*AAVAAFVANHVEVHLRDFVAVVAKNPNLVTVHRRHNCCF 175
Query: 751 DLLIQHFPMMCQIKSCFRAYWTSVFVMRHHFFKTCQVHCVSTVQNSCSL**VK*ILI 581
LLIQH P+MC +KS RAYWT VF+M HH KT VH VST +NSC **+K*IL+
Sbjct: 174 YLLIQHAPVMCHVKSRLRAYWTRVFIMFHHLRKTVHVHGVSTGKNSCPF**IK*ILV 4
Score = 130 bits (279), Expect(2) = 4e-031
Identities = 59/115 (51%), Positives = 68/115 (59%)
Frame = +3 / +2
Query: 588 IYLTHYKEQEF*TVDTQCT*HVLKKWWRITNTLVQYALKQLLI*HIIGKCWIKRSKPRSC 767
IYL H K QEF VDT CT V +WW I NTLVQYA ++LL *HI G CWIKR K + C
Sbjct: 11 IYLIH*KGQEFLPVDTPCTWTVFLRWWNIINTLVQYARRRLLT*HITGACWIKR*KQQLC 190
Query: 768 LLCIATRFGCFATIATRSQRXXXXXXXXXXXXXXXXXPEQHRALQIYPEAAHLQQ 932
LLC TRFG FAT AT+S+R ++ R LQIY EA +Q
Sbjct: 191 LLCTVTRFGFFATTATKSRRWTST*LATNAATAAHTTLDRPRGLQIYQEAVLRRQ 355
Score = 112 bits (240), Expect = 1e-025
Identities = 53/86 (61%), Positives = 59/86 (68%)
Frame = -2 / -1
Query: 845 QSRESSPLRPCCNRCKAPKSCSDTQEA*SWLRSLDPTFPNDVSNQELF*SILDKCICYAP 666
QSR S P R CC+RCK PK C+ TQEA* L SLDPT P+DVS QE SILDK I Y P
Sbjct: 268 QSRGSPPPRLCCSRCKEPKPCNGTQEA*LLLLSLDPTCPSDVSCQEPSSSILDKSIYYVP 89
Query: 665 PFLQNMSSALCVHSSELLFLVMSQID 588
P +N A CVH ELL L+M+QID
Sbjct: 88 PSQKNSPCAWCVHR*ELLSLLMNQID 11
Score = 84.5 bits (178), Expect(2) = 2e-027
Identities = 41/63 (65%), Positives = 50/63 (79%)
Frame = +1 / +3
Query: 589 SI*LITRNKSSELWTHNALDMF*RNGGA*QIHLSNML*NSS*FDTSLGNVGSRDRSHDHA 768
SI* I R+KSS LWTH+A +F +GG * I LSNML + S* DTSLG+VGSRDRS+++A
Sbjct: 12 SI*FIKRDKSSYLWTHHAHGLFF*DGGT**ILLSNMLEDGS*HDTSLGHVGSRDRSNNYA 191
Query: 769 SCV 777
SCV
Sbjct: 192 SCV 200
Score = 53.8 bits (111), Expect(2) = 2e-027
Identities = 18/28 (64%), Positives = 24/28 (85%)
Frame = +1 / +3
Query: 841 DWPQVQPLQIVQHPNNIAPCRFIRKQLT 924
DWPQ+QPLQ++QH ++A CRFIRKQ +
Sbjct: 264 DWPQMQPLQLIQHSIDLAACRFIRKQFS 347
Score = 23.1 bits (44), Expect(2) = 4e-031
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = +3 / +1
Query: 1194 FYHCG*NVITYLL 1232
+YHCG*+ Y+L
Sbjct: 658 YYHCG*SPACYIL 696
Score = 22.6 bits (43), Expect(2) = 7e-066
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = +3 / +2
Query: 990 EPEEETLFRGV 1022
E EEE LFRG+
Sbjct: 440 EEEEEALFRGI 472
>gb|CN816242.1|CN816242 HRO4518_E06_I12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4518_E06_I12, mRNA sequence
Length = 723
Score = 121 bits (259), Expect(2) = 2e-037
Identities = 41/76 (53%), Positives = 54/76 (71%)
Frame = +2 / +3
Query: 524 LRDNHQCVENSMRQNCPICYEYLFDSLQGTRVLNCGHTMHLTCFEEMVAHNKYTCPICSK 703
+ D+H CVE +M +CPIC+EYLFDS+ G VL CGHT+H C +EM H K CP+CSK
Sbjct: 9 VEDSHACVEGAMHHDCPICFEYLFDSIDGVSVLRCGHTIHENCLKEMEKHRKLICPLCSK 188
Query: 704 TALDLTHHWEMLDQEI 751
+ D++ WE LD+EI
Sbjct: 189 SVCDMSRAWERLDREI 236
Score = 50.6 bits (104), Expect(2) = 2e-037
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = +2 / +3
Query: 794 VLCNDCNKVSEVNFHVIGHKCSHCRSYNTR 883
+LCN+C ++EV F++I KC C+SYNTR
Sbjct: 276 ILCNECEAIAEVRFNLIALKCQSCQSYNTR 365
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 18,633,545
Number of Sequences: 8143
Number of extensions: 318249
Number of successful extensions: 1736
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 520055516
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)