TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440015.2.1
(608 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818423.1|CN818423 HRO4453_C09_F17ZS5 Lib AA070E1X Aven... 169 3e-043
gb|CN820760.1|CN820760 HRO4414_D11_G22ZS5 Lib AA069E1X Aven... 168 8e-043
>gb|CN818423.1|CN818423 HRO4453_C09_F17ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4453_C09_F17, mRNA sequence
Length = 619
Score = 169 bits (364), Expect = 3e-043
Identities = 72/93 (77%), Positives = 77/93 (82%)
Frame = -3 / +3
Query: 564 EQTLPPPQLRVPXXXXXAKMIIPVRCFTCGKVIGNKWDLYLDLLQADYSEGDALDALELF 385
E LP L +KMIIPVRCFTCGKVIGNKWD YLDLLQADY+EGDALDAL L
Sbjct: 12 ETLLPLSFLSSGGVVAASKMIIPVRCFTCGKVIGNKWDHYLDLLQADYTEGDALDALGLV 191
Query: 384 RYCCRRMLMTHVDLIEKLLNYNTLEKTETS*VS 286
RYCCRRMLMTHVDLIEKLLNYNTLEKTET+*++
Sbjct: 192 RYCCRRMLMTHVDLIEKLLNYNTLEKTETT*LN 290
Score = 103 bits (220), Expect = 2e-023
Identities = 48/88 (54%), Positives = 56/88 (63%)
Frame = +1 / -3
Query: 292 LTCLSLLQCVVVEQLLNEVNMGHEHSPAAVAEQFQSIQSIPFRXXXXXXXXXXXXXXXNH 471
L+CL LQCVVVEQLL++VNMGHEH+ AAVA Q Q IQSIPFR +H
Sbjct: 284 LSCLRFLQCVVVEQLLDKVNMGHEHTTAAVANQAQGIQSIPFRVIGLEKIKVVVPLVTDH 105
Query: 472 LAAGEAAHRYDHLXXXXXSWNPELGRGK 555
LAAGEAAH DHL + + G+GK
Sbjct: 104 LAAGEAAHGDDHLRRCDYAAAGQEGKGK 21
Score = 71.2 bits (149), Expect(2) = 2e-021
Identities = 31/43 (72%), Positives = 34/43 (79%)
Frame = +3 / -2
Query: 288 SLNLSQSSPVCCS*ATSQ*GQHGS*AFACSSSGTIPEHPEHPL 416
S+ LS SPVCCS*ATS+*GQHGS*A+ SSS P HPEHPL
Sbjct: 288 SIKLSPFSPVCCS*ATSR*GQHGS*AYDGSSSEPSPRHPEHPL 160
Score = 45.5 bits (93), Expect(2) = 2e-021
Identities = 18/19 (94%), Positives = 18/19 (94%)
Frame = +2 / -1
Query: 455 HLFPITLPQVKQRTGMIIF 511
HL PITLPQVKQRTGMIIF
Sbjct: 121 HLLPITLPQVKQRTGMIIF 65
>gb|CN820760.1|CN820760 HRO4414_D11_G22ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_D11_G22, mRNA
sequence
Length = 498
Score = 168 bits (361), Expect = 8e-043
Identities = 68/75 (90%), Positives = 72/75 (96%)
Frame = -3 / +2
Query: 510 KMIIPVRCFTCGKVIGNKWDLYLDLLQADYSEGDALDALELFRYCCRRMLMTHVDLIEKL 331
KMIIPVRCFTCGKVIGNKWD YLDLLQADY+EGDALDAL L RYCCRRMLMTHVDLIEKL
Sbjct: 44 KMIIPVRCFTCGKVIGNKWDHYLDLLQADYTEGDALDALGLVRYCCRRMLMTHVDLIEKL 223
Query: 330 LNYNTLEKTETS*VS 286
LNYNTLEKTET+*++
Sbjct: 224 LNYNTLEKTETT*LN 268
Score = 100 bits (214), Expect = 2e-022
Identities = 45/73 (61%), Positives = 50/73 (68%)
Frame = +1 / -3
Query: 292 LTCLSLLQCVVVEQLLNEVNMGHEHSPAAVAEQFQSIQSIPFRXXXXXXXXXXXXXXXNH 471
L+CL LQCVVVEQLL++VNMGHEH+ AAVA Q Q IQSIPFR +H
Sbjct: 262 LSCLRFLQCVVVEQLLDKVNMGHEHTTAAVANQAQGIQSIPFRVIGLEKIKVVVPLVTDH 83
Query: 472 LAAGEAAHRYDHL 510
LAAGEAAH DHL
Sbjct: 82 LAAGEAAHGDDHL 44
Score = 71.2 bits (149), Expect(2) = 2e-021
Identities = 31/43 (72%), Positives = 34/43 (79%)
Frame = +3 / -2
Query: 288 SLNLSQSSPVCCS*ATSQ*GQHGS*AFACSSSGTIPEHPEHPL 416
S+ LS SPVCCS*ATS+*GQHGS*A+ SSS P HPEHPL
Sbjct: 266 SIKLSPFSPVCCS*ATSR*GQHGS*AYDGSSSEPSPRHPEHPL 138
Score = 45.5 bits (93), Expect(2) = 2e-021
Identities = 18/19 (94%), Positives = 18/19 (94%)
Frame = +2 / -1
Query: 455 HLFPITLPQVKQRTGMIIF 511
HL PITLPQVKQRTGMIIF
Sbjct: 99 HLLPITLPQVKQRTGMIIF 43
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,488,060
Number of Sequences: 8143
Number of extensions: 82490
Number of successful extensions: 1475
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 196076960
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)