TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2430946.2.3
(3896 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Aven... 198 7e-051
gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Aven... 195 5e-050
gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Aven... 145 4e-035
gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Aven... 100 1e-021
>gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_G06_M12, mRNA sequence
Length = 521
Score = 198 bits (426), Expect = 7e-051
Identities = 73/96 (76%), Positives = 92/96 (95%)
Frame = -2 / +2
Query: 820 KWTSLLIPPTTVLVINLVGMVAGISYAINSGYQSWGPLFGKLFFSIWVILHLYPFLKGLM 641
+WT+LLIPPTT+++IN+VG+VAG+S A+N+GY SWGPLFGKLFFS WVI+HLYPFLKGLM
Sbjct: 11 QWTTLLIPPTTLIIINMVGIVAGVSDAVNNGYGSWGPLFGKLFFSFWVIVHLYPFLKGLM 190
Query: 640 GRQNRTPTIVIVWSILLASIFSLLWVKIDPFISPTQ 533
GRQNRTPTIV++WS+LLASIFSL+WV+IDPFI+ ++
Sbjct: 191 GRQNRTPTIVVLWSVLLASIFSLVWVRIDPFIAKSK 298
Score = 136 bits (292), Expect = 2e-032
Identities = 59/91 (64%), Positives = 71/91 (78%)
Frame = +3 / -3
Query: 546 MKGSIFTHSKEKIDARRMDQTMTIVGVRFCLPMRPLRKG*R*RITQIEKNSFPKSGPQDW 725
MKGSI T ++EK++A R DQ+ T+VGVRFCLPM PLR G*R +TQ EK SFPK GP D
Sbjct: 285 MKGSILTQTREKMEASRTDQSTTMVGVRFCLPMSPLRNG*RWTMTQKEKKSFPKRGPHDP 106
Query: 726 *PLLMAYEIPATIPTRLMTRTVVGGMSKLVH 818
*PLL A E PAT+PT LM +VVGG+S++VH
Sbjct: 105 *PLLTASETPATMPTMLMMMSVVGGISRVVH 13
>gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C09_F17, mRNA
sequence
Length = 676
Score = 195 bits (420), Expect = 5e-050
Identities = 76/88 (86%), Positives = 85/88 (96%)
Frame = -2 / +1
Query: 811 SLLIPPTTVLVINLVGMVAGISYAINSGYQSWGPLFGKLFFSIWVILHLYPFLKGLMGRQ 632
+LLIPPTT+L+IN+VG+VAG SYAINSGYQSWGPLFGKLFF+ WVI+HLYPFLKGLMGR
Sbjct: 13 TLLIPPTTILIINMVGVVAGTSYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRT 192
Query: 631 NRTPTIVIVWSILLASIFSLLWVKIDPF 548
NRTPTIVIVW+ILLASIFSLLWV+IDPF
Sbjct: 193 NRTPTIVIVWAILLASIFSLLWVRIDPF 276
Score = 141 bits (303), Expect = 6e-034
Identities = 62/86 (72%), Positives = 69/86 (80%)
Frame = +3 / -3
Query: 546 MKGSIFTHSKEKIDARRMDQTMTIVGVRFCLPMRPLRKG*R*RITQIEKNSFPKSGPQDW 725
+ GSI THSKEKI+ARR+ QTMTIVGVRF LP+RPLR G +* ITQ K SFP SGP DW
Sbjct: 278 VNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TITQKAKKSFPNSGPHDW 99
Query: 726 *PLLMAYEIPATIPTRLMTRTVVGGM 803
*PLLMA E+PAT PT LM + VVGGM
Sbjct: 98 *PLLMA*EVPATTPTMLMIKIVVGGM 21
Score = 101 bits (216), Expect = 6e-022
Identities = 44/89 (49%), Positives = 64/89 (71%)
Frame = +2 / -2
Query: 542 GDERIDLHPQ*GEDRCKEDGPNDDDCWRAVLPAHETLEEGVEMKDHPDREEQLSKERTPG 721
G E I+ +PQ GEDR +ED P+DD+ AV PAH+TLEE V + +HP+ +E+LS++R P
Sbjct: 282 GSEWINTNPQQGEDRSEEDSPDDDNRRCAVCPAHKTLEEWV*VNNHPEGKEELSEQRPP* 103
Query: 722 LVAAVNGIRNSCHHSDQVNDKNSGRRDEQ 808
LV V+GI + +++D VND+N RDE+
Sbjct: 102 LVTTVDGIGGASNNTDHVNDQNCCWRDEK 16
>gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_H12_O23, mRNA
sequence
Length = 620
Score = 145 bits (312), Expect = 4e-035
Identities = 56/62 (90%), Positives = 60/62 (96%)
Frame = -2 / +2
Query: 733 SGYQSWGPLFGKLFFSIWVILHLYPFLKGLMGRQNRTPTIVIVWSILLASIFSLLWVKID 554
SGYQSWGPLFGKLFF+ WVI+HLYPFLKGLMGR NRTPTIVIVW+ILLASIFSLLWV+ID
Sbjct: 2 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILLASIFSLLWVRID 181
Query: 553 PF 548
PF
Sbjct: 182 PF 187
Score = 108 bits (231), Expect = 5e-024
Identities = 47/63 (74%), Positives = 52/63 (82%)
Frame = +3 / -3
Query: 546 MKGSIFTHSKEKIDARRMDQTMTIVGVRFCLPMRPLRKG*R*RITQIEKNSFPKSGPQDW 725
+ GSI THSKEKI+ARR+ QTMTIVGVRF LP+RPLR G +* ITQ K SFPKSGP DW
Sbjct: 189 VNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TITQKAKKSFPKSGPHDW 10
Query: 726 *PL 734
*PL
Sbjct: 9 *PL 1
>gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_A01_A02, mRNA sequence
Length = 474
Score = 100 bits (214), Expect = 1e-021
Identities = 39/48 (81%), Positives = 46/48 (95%)
Frame = -2 / +3
Query: 679 VILHLYPFLKGLMGRQNRTPTIVIVWSILLASIFSLLWVKIDPFISPT 536
VI+HLYPFLKGL+GRQNRTPTIVIVWSILLASI SLLWV+++PF++ T
Sbjct: 12 VIVHLYPFLKGLLGRQNRTPTIVIVWSILLASIISLLWVRVNPFLAKT 155
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 45,954,136
Number of Sequences: 8143
Number of extensions: 682763
Number of successful extensions: 2716
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 47
effective length of database: 1,184,766
effective search space used: 1482142266
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)