TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419471.2.3
(1052 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816713.1|CN816713 HRO4523_H09_P17ZS5 Lib AA071E1X Aven... 177 3e-061
gb|BE439203.1|BE439203 CDO1421_WHE03E02R ITEC CDO Oat cDNA ... 181 2e-046
gb|CN815566.1|CN815566 HRO4513_B02_C03ZS5 Lib AA071E1X Aven... 94 5e-036
gb|CN817098.1|CN817098 HRO4528_B12_D24ZS5 Lib AA071E1X Aven... 89 3e-035
gb|CN821529.1|CN821529 HRO4426_D02_G04ZS5 Lib AA069E1X Aven... 73 3e-026
>gb|CN816713.1|CN816713 HRO4523_H09_P17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4523_H09_P17, mRNA sequence
Length = 608
Score = 177 bits (382), Expect(2) = 3e-061
Identities = 74/83 (89%), Positives = 80/83 (96%)
Frame = -3 / +2
Query: 666 KVDRSGAYVARQAAKSIVAAGLARRAIVQVSYAIGVPEPLSVFVDTYGTGAIPDKEILKI 487
KVDRSGAY+ARQAAKSIVA+GLARR IVQ+SYAIGVPEPLSVFVD+YGTG IPD+EILK+
Sbjct: 17 KVDRSGAYIARQAAKSIVASGLARRVIVQISYAIGVPEPLSVFVDSYGTGKIPDREILKL 196
Query: 486 VKENFDFRPGMIIINLDLKKGGN 418
VKENFDFRPGMI INLDLKKGGN
Sbjct: 197 VKENFDFRPGMISINLDLKKGGN 265
Score = 83.5 bits (176), Expect(2) = 1e-024
Identities = 36/44 (81%), Positives = 39/44 (88%)
Frame = +2 / -3
Query: 446 MMIMPGLKSKFSFTIFRISLSGIAPVPYVSTNTDRGSGTPMA*E 577
M+I+PGLKSKFSFT FRISLSGI PVP STNTD+GSGTPMA E
Sbjct: 237 MLIIPGLKSKFSFTSFRISLSGILPVP*ESTNTDKGSGTPMAYE 106
Score = 73.0 bits (153), Expect(2) = 3e-061
Identities = 27/35 (77%), Positives = 30/35 (85%)
Frame = -3 / +2
Query: 429 KGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKAEK 325
K G R++KTAAYGHFGRDD DFTWEVVKPLK +K
Sbjct: 251 KKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDK 355
Score = 45.1 bits (92), Expect(2) = 1e-024
Identities = 22/28 (78%), Positives = 22/28 (78%)
Frame = +2 / -3
Query: 320 DGFSALRGFTTSQVKSGSSLPKCP*AAV 403
D S LRGFTTSQVKS SS PK P*AAV
Sbjct: 360 DVLSNLRGFTTSQVKSASSRPK*P*AAV 277
>gb|BE439203.1|BE439203 CDO1421_WHE03E02R ITEC CDO Oat cDNA Library Avena sativa cDNA clone
CDO1421_WHE03E02, mRNA sequence
Length = 275
Score = 181 bits (390), Expect = 2e-046
Identities = 72/79 (91%), Positives = 74/79 (93%)
Frame = -3 / +3
Query: 558 PEPLSVFVDTYGTGAIPDKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFG 379
PEPLSVFVDTYGTG IPDK+IL IVKENFDFRPGMIIINLDLK+GGNGRYLKTAAYGHFG
Sbjct: 3 PEPLSVFVDTYGTGRIPDKDILNIVKENFDFRPGMIIINLDLKRGGNGRYLKTAAYGHFG 182
Query: 378 RDDPDFTWEVVKPLKAEKP 322
RD PDFTWEVVKPLK E P
Sbjct: 183 RDGPDFTWEVVKPLKWENP 239
Score = 78.0 bits (164), Expect(2) = 1e-026
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +1 / -1
Query: 397 RRLEVAPVAAFLEVEVDDDHAGPEVEVLLHDLQDLLVGDRAGAVRVHEHR 546
RRLEV P+ L+VEVDDDHAGPEV+VLL +++D+LV D A AV VH+HR
Sbjct: 164 RRLEVPPIPTPLQVEVDDDHAGPEVKVLLDNVEDILVRDPACAVCVHKHR 15
Score = 57.0 bits (118), Expect(2) = 1e-026
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = +2 / -2
Query: 320 DGFSALRGFTTSQVKSGSSLPKCP*AAV 403
+GFS LRGFTTSQVKSG SLPKCP AAV
Sbjct: 241 EGFSHLRGFTTSQVKSGPSLPKCPYAAV 158
>gb|CN815566.1|CN815566 HRO4513_B02_C03ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4513_B02_C03, mRNA sequence
Length = 656
Score = 93.6 bits (198), Expect(2) = 5e-036
Identities = 37/42 (88%), Positives = 40/42 (95%)
Frame = -3 / +1
Query: 543 VFVDTYGTGAIPDKEILKIVKENFDFRPGMIIINLDLKKGGN 418
VFVD+YGTG IPD+EILK+VKENFDFRPGMI INLDLKKGGN
Sbjct: 10 VFVDSYGTGKIPDREILKLVKENFDFRPGMISINLDLKKGGN 135
Score = 73.0 bits (153), Expect(2) = 5e-036
Identities = 27/35 (77%), Positives = 30/35 (85%)
Frame = -3 / +1
Query: 429 KGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKAEK 325
K G R++KTAAYGHFGRDD DFTWEVVKPLK +K
Sbjct: 121 KKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDK 225
>gb|CN817098.1|CN817098 HRO4528_B12_D24ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_B12_D24, mRNA sequence
Length = 718
Score = 89.0 bits (188), Expect(2) = 3e-035
Identities = 35/39 (89%), Positives = 37/39 (94%)
Frame = -3 / +3
Query: 534 DTYGTGAIPDKEILKIVKENFDFRPGMIIINLDLKKGGN 418
DTYGTG IPD+EILK+VKENFDFRPGMI INLDLKKGGN
Sbjct: 12 DTYGTGKIPDREILKLVKENFDFRPGMISINLDLKKGGN 128
Score = 74.8 bits (157), Expect(2) = 3e-035
Identities = 27/35 (77%), Positives = 31/35 (88%)
Frame = -3 / +3
Query: 429 KGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKAEK 325
K G R++KTAAYGHFGR+DPDFTWEVVKPLK +K
Sbjct: 114 KKGGNRFIKTAAYGHFGREDPDFTWEVVKPLKFDK 218
Score = 70.7 bits (148), Expect(3) = 3e-022
Identities = 31/40 (77%), Positives = 33/40 (82%)
Frame = +1 / -3
Query: 415 PVAAFLEVEVDDDHAGPEVEVLLHDLQDLLVGDRAGAVRV 534
PV A LEV+VD DH GPEVEVLLH+LQDL VGD AGAV V
Sbjct: 131 PVPALLEVKVDADHPGPEVEVLLHELQDLPVGDLAGAVGV 12
Score = 49.2 bits (101), Expect(3) = 3e-022
Identities = 23/30 (76%), Positives = 24/30 (80%)
Frame = +2 / -1
Query: 314 AEDGFSALRGFTTSQVKSGSSLPKCP*AAV 403
A+ S LRGFTTSQVKSGSS PK P*AAV
Sbjct: 229 ADAALSNLRGFTTSQVKSGSSRPKWP*AAV 140
Score = 22.1 bits (42), Expect(3) = 3e-022
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = +1 / -1
Query: 1 FFFFFFLTKMH 33
FFFFFF +H
Sbjct: 505 FFFFFFFFXIH 473
>gb|CN821529.1|CN821529 HRO4426_D02_G04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4426_D02_G04, mRNA
sequence
Length = 515
Score = 73.0 bits (153), Expect(2) = 3e-026
Identities = 27/35 (77%), Positives = 30/35 (85%)
Frame = -3 / +1
Query: 429 KGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKAEK 325
K G R++KTAAYGHFGRDD DFTWEVVKPLK +K
Sbjct: 64 KKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDK 168
Score = 60.6 bits (126), Expect(2) = 3e-026
Identities = 24/26 (92%), Positives = 25/26 (96%)
Frame = -3 / +1
Query: 495 LKIVKENFDFRPGMIIINLDLKKGGN 418
LK+VKENFDFRPGMI INLDLKKGGN
Sbjct: 1 LKLVKENFDFRPGMISINLDLKKGGN 78
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,375,804
Number of Sequences: 8143
Number of extensions: 176291
Number of successful extensions: 3086
Number of sequences better than 1.0e-020: 6
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 370013670
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)