TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2418861.2.1
(614 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN814661.1|CN814661 HRO4501_B06_C11ZS5 Lib AA071E1X Aven... 308 5e-085
>gb|CN814661.1|CN814661 HRO4501_B06_C11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4501_B06_C11, mRNA sequence
Length = 842
Score = 308 bits (667), Expect = 5e-085
Identities = 123/130 (94%), Positives = 126/130 (96%)
Frame = +3 / +2
Query: 225 ARKSNYTDMVNKYYDLATSFYEYGWGESFHFAHRWNGESLRESIKRHEHFLALQLGLKPG 404
ARK NYTDMVNKYYDL+TSFYEYGWGESFHFAHRWNGESLRESIKRHEHFLALQL LKPG
Sbjct: 50 ARKFNYTDMVNKYYDLSTSFYEYGWGESFHFAHRWNGESLRESIKRHEHFLALQLELKPG 229
Query: 405 MKVLDVGCGIGGPLREIARFSSTSVTGLNNNEYQITRGKELNRLAGISGTCDFVKADFMK 584
MKVLDVGCGIGGPLREIARFSSTSVTGLNNN+YQITRGKELNRLAG+SGTCDFVKADFM
Sbjct: 230 MKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQITRGKELNRLAGLSGTCDFVKADFMN 409
Query: 585 MPFDDNTFDA 614
MPF DNTFDA
Sbjct: 410 MPFSDNTFDA 439
Score = 249 bits (539), Expect = 2e-067
Identities = 115/130 (88%), Positives = 118/130 (90%)
Frame = -2 / -3
Query: 613 ASKVLSSNGIFMKSALTKSHVPLIPAKRLSSFPLVIWYSLLFNPVTEVELNLAISLSGPP 434
ASKVLS GIFMKSALTKS VPL PA+RLSSFPLVI SLLFNPVTEVELNLAISL+GPP
Sbjct: 438 ASKVLSEKGIFMKSALTKSQVPLSPARRLSSFPLVI**SLLFNPVTEVELNLAISLNGPP 259
Query: 433 IPQPTSKTFIPGFKPSCRARKCSCRLMLSRKDSPFHL*AKWKDSPQPYS*KLVARS*YLL 254
IPQPTSKTFIPGF S RARKCSCRLMLS KDSPFHL*AK KDSPQPYS*KLV RS*YLL
Sbjct: 258 IPQPTSKTFIPGFNSS*RARKCSCRLMLSLKDSPFHL*AK*KDSPQPYS*KLVERS*YLL 79
Query: 253 TISV*LDFLA 224
TISV*L+FLA
Sbjct: 78 TISV*LNFLA 49
Score = 234 bits (506), Expect = 9e-063
Identities = 101/141 (71%), Positives = 116/141 (82%)
Frame = -1 / -2
Query: 614 SIKSVVIERHLHEVRLDKITCSTNSC*TVELLSPGYLVFVVIQSGN*S*AKSCNFSQWST 435
SIKSV+ ERH+HEV LDK+T ST SC T+ELLSP YL+ VV+QSGN* *AKS N S WST
Sbjct: 439 SIKSVIGERHIHEVCLDKVTSSTKSCQTIELLSPSYLIVVVVQSGN*G*AKSGNLS*WST 260
Query: 434 YSTAHI*NLHSWFQTKLQGKKMLMSLDAFT*GFSIPSVSKVEGFTPTILIEASGKIIVFI 255
YSTAH+ NLH WFQ KL+G+KML+S DAF * F IP VSKV+ FTP +LIEAS KI+VFI
Sbjct: 259 YSTAHVQNLHPWFQLKLKGQKMLVSFDAFP*RFPIPPVSKVKRFTPAVLIEASRKIVVFI 80
Query: 254 NHISIVGLPCFLLPSIVSMIF 192
NHIS+V LPCFLLPS+VS F
Sbjct: 79 NHISVVELPCFLLPSVVSRNF 17
Score = 194 bits (418), Expect = 1e-050
Identities = 93/137 (67%), Positives = 102/137 (74%)
Frame = -3 / -1
Query: 612 HQKCCHRTASS*SPP*QNHMFH*FLLNG*APFPWLSGIRCYSIR*LKLS*ILQFLSVVHL 433
HQKC R A S*S P*Q+H FH* L + *APFP LS RC SIR*L+LS*I Q L +VHL
Sbjct: 437 HQKCYRRKAYS*SLP*QSHKFH*VLPDD*APFP*LSDSRCCSIR*LRLS*IWQSLLMVHL 258
Query: 432 FHSPHLKPSFLVSNQVAGQENAHVA*CFHVRILHSICEQSGRIHPNHTHRS*WQDHSIY* 253
FHSP KPS LVS Q G ENA V *CF ++I HS CEQS +IHP+ THRS* +D SIY*
Sbjct: 257 FHSPRPKPSSLVSTQAEGPENARVV*CFPLKIPHSTCEQSEKIHPSRTHRS**KDRSIY* 78
Query: 252 PYQYSWTSLLPPSLHSI 202
PYQ S TSLLPPSL SI
Sbjct: 77 PYQCS*TSLLPPSLRSI 27
Score = 146 bits (313), Expect = 4e-036
Identities = 77/120 (64%), Positives = 82/120 (68%)
Frame = +1 / +3
Query: 199 MDTMEGRRKQGSPTILIWLINTMILPLASMSMVGVNPSTLLTDGMENPYVKASSDMSIFL 378
+DT EGRRKQGS T LIWLINT I LASMS GVN TLLT GM N KAS+D SIF
Sbjct: 24 LDTTEGRRKQGSSTTLIWLINTTIFLLASMSTAGVNLFTLLTGGMGNL*GKASNDTSIFW 203
Query: 379 PCNLV*NQE*RF*MWAVE*VDH*EKXXXXXXXXXPD*ITTNTR*PGERSSTV*QELVEHV 558
P +L *NQ *RF WAVE*VDH*E+ PD* TT R* GERSS V Q+LVE V
Sbjct: 204 PFSLS*NQG*RFWTWAVE*VDH*ERLPDLAQPQLPD*TTTTIR*LGERSSIVWQDLVELV 383
Score = 144 bits (310), Expect(2) = 3e-043
Identities = 63/99 (63%), Positives = 71/99 (71%)
Frame = +2 / +1
Query: 317 CSQMEWRILT*KHQAT*AFSCPATWFETRNEGFRCGLWNRWTTERNCKI*LNFSYRIE*Q 496
CSQ+EW I KHQ T AFS P+ ETR+EGF GLWNRWT +R+C+I*LN SYRIE Q
Sbjct: 142 CSQVEWGIFKGKHQTTRAFSGPSA*VETRDEGFGRGLWNRWTIKRDCQI*LNLSYRIEQQ 321
Query: 497 RIPDNQGKGAQPFSRN*WNM*FCQGGLHEDAVR*QHF*C 613
R+ DN GKGAQ R *WN+* CQG LHE A HF*C
Sbjct: 322 RLSDN*GKGAQSSGRT*WNL*LCQGRLHEYAFLR*HF*C 438
Score = 45.1 bits (92), Expect(2) = 3e-043
Identities = 22/27 (81%), Positives = 24/27 (88%)
Frame = +2 / +1
Query: 200 WILWREGGSKEVQLY*YG**IL*SCH* 280
WIL REGGSKEVQL+*YG**IL S +*
Sbjct: 25 WILRREGGSKEVQLH*YG**ILRSFY* 105
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,909,298
Number of Sequences: 8143
Number of extensions: 79419
Number of successful extensions: 1055
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 198527922
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)