TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2405118.2.1
(1669 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815915.1|CN815915 HRO4511_H05_P09ZS5 Lib AA071E1X Aven... 77 1e-025
>gb|CN815915.1|CN815915 HRO4511_H05_P09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_H05_P09, mRNA sequence
Length = 842
Score = 77.1 bits (162), Expect(2) = 1e-025
Identities = 29/64 (45%), Positives = 43/64 (67%)
Frame = -2 / -3
Query: 675 LGRKAITGSFIGSIDETAEVLQFCVDKGLTSQIEVVKMGYVNEALERLERNDVRYRFVVD 496
LG ++++GS G +T E++ FC + IE++K+ Y+NEALERL DVRYRFV+D
Sbjct: 456 LGARSLSGSVTGGTKDTQEMMNFCAANKIYPDIELIKIDYINEALERLVNRDVRYRFVID 277
Query: 495 VAGS 484
+ S
Sbjct: 276 IESS 265
Score = 55.6 bits (115), Expect(2) = 1e-025
Identities = 25/65 (38%), Positives = 33/65 (50%)
Frame = -2 / -3
Query: 954 VKVAKAMGHHVTVISSSSKKRAEAMDHLGADAYLVXXXXXXXXXXXXXXDYIIDTVPVHH 775
VK KA G VTV S+S KR EA+ LGAD +++ +I+DT H
Sbjct: 732 VKFGKAFGLKVTVFSTSESKRDEAISLLGADNFVLSSDEKQMGSLKNSLHFIVDTASGDH 553
Query: 774 PLEPY 760
P +PY
Sbjct: 552 PFDPY 538
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,951,728
Number of Sequences: 8143
Number of extensions: 145568
Number of successful extensions: 1120
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 612536520
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)