TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2405110.2.1
(945 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819537.1|CN819537 HRO4401_C11_E21ZS5 Lib AA069E1X Aven... 79 1e-024
gb|CN821289.1|CN821289 HRO4423_F05_L09ZS5 Lib AA069E1X Aven... 79 2e-024
gb|CN817131.1|CN817131 HRO4528_E12_J24ZS5 Lib AA071E1X Aven... 96 9e-021
>gb|CN819537.1|CN819537 HRO4401_C11_E21ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_C11_E21, mRNA
sequence
Length = 657
Score = 79.0 bits (166), Expect(3) = 1e-024
Identities = 29/49 (59%), Positives = 36/49 (73%)
Frame = -1 / +3
Query: 756 HLTGTIAGPADSPYEGGTFVIDIRLPGGYPFEPPKMQFITKVWHPNISS 610
H TI GP DSPY GG F+++I P YPF+PPK+ F TKV+HPNI+S
Sbjct: 15 HWLPTIMGPPDSPYAGGVFLVNIHFPPDYPFKPPKVSFKTKVFHPNINS 161
Score = 34.1 bits (68), Expect(3) = 1e-024
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = -1 / +3
Query: 612 SQNGAICLDILKDQW 568
+ NG+ICLDILK+QW
Sbjct: 156 NSNGSICLDILKEQW 200
Score = 32.7 bits (65), Expect(3) = 1e-024
Identities = 14/42 (33%), Positives = 19/42 (45%)
Frame = -1 / +3
Query: 501 DDPQDAVVAQQYLRDYPTFAATARYWTEAFAKSASTGMEEKV 376
DDP +A Y D + TAR WT+ +A T + V
Sbjct: 267 DDPLVPEIAHMYKTDRSKYETTARSWTQKYAMG*RTALRTTV 392
>gb|CN821289.1|CN821289 HRO4423_F05_L09ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4423_F05_L09, mRNA
sequence
Length = 648
Score = 79.4 bits (167), Expect(3) = 2e-024
Identities = 28/49 (57%), Positives = 36/49 (73%)
Frame = -1 / -2
Query: 756 HLTGTIAGPADSPYEGGTFVIDIRLPGGYPFEPPKMQFITKVWHPNISS 610
H TI GP+DSPY GG F++ I P YPF+PPK+ F TKV+HPN++S
Sbjct: 572 HWQATIMGPSDSPYSGGVFLVTIHFPPDYPFKPPKVAFKTKVFHPNVNS 426
Score = 34.1 bits (68), Expect(3) = 2e-024
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = -1 / -2
Query: 612 SQNGAICLDILKDQW 568
+ NG+ICLDILK+QW
Sbjct: 431 NSNGSICLDILKEQW 387
Score = 31.8 bits (63), Expect(3) = 2e-024
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -1 / -2
Query: 501 DDPQDAVVAQQYLRDYPTFAATARYWTEAFA 409
DDP +A Y D + +TAR WT+ +A
Sbjct: 320 DDPLVPEIAHMYKADRTKYESTARSWTQKYA 228
>gb|CN817131.1|CN817131 HRO4528_E12_J24ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_E12_J24, mRNA sequence
Length = 726
Score = 95.9 bits (203), Expect = 9e-021
Identities = 36/89 (40%), Positives = 55/89 (61%)
Frame = -1 / +2
Query: 834 KELTECNRDREVSGVSIALHDGANISHLTGTIAGPADSPYEGGTFVIDIRLPGGYPFEPP 655
KE E R++ + D +NI T I GP+++P+EGG F + +P YP PP
Sbjct: 41 KEYKEVQREKSADPDIQLICDDSNIFKWTALIKGPSETPFEGGVFQLAFSIPEQYPLLPP 220
Query: 654 KMQFITKVWHPNISSQNGAICLDILKDQW 568
+++F+TK++HPN+ + G ICLDILK+ W
Sbjct: 221 QVRFLTKIFHPNVHFKTGEICLDILKNAW 307
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,832,118
Number of Sequences: 8143
Number of extensions: 138386
Number of successful extensions: 1097
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 329898598
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)