TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2405087.2.1
(737 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816530.1|CN816530 HRO4521_G10_M19ZS5 Lib AA071E1X Aven... 426 e-120
>gb|CN816530.1|CN816530 HRO4521_G10_M19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4521_G10_M19, mRNA sequence
Length = 749
Score = 426 bits (925), Expect = e-120
Identities = 185/228 (81%), Positives = 199/228 (87%)
Frame = +2 / +1
Query: 53 KNYLSRILTLGPRSPAIGNREERSNTPISGRKNIIVISVDSVNKEGLVRIIRNAIEVIHK 232
KN+LSRILTLG RSPAIG++EERSN PIS RK+IIVISVDSVNKE LVRIIRNAIE H
Sbjct: 13 KNHLSRILTLGARSPAIGSKEERSNAPIS*RKHIIVISVDSVNKEDLVRIIRNAIEAAHT 192
Query: 233 ENMSGSTGFVLSTSLTISEIHSLLLSGGMLPTDFDAFICNSGSNIYYPSHSRETSNNSKI 412
EN STGFVLSTSLTISEI SLL+S GM P FDAFICNSGS+IYYPS+S T +SK+
Sbjct: 193 ENTPASTGFVLSTSLTISEICSLLVSVGMHPAGFDAFICNSGSSIYYPSYSGNTPTSSKV 372
Query: 413 TFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYCLAFRV 592
T + QNHQSHIEYRWGGEGLRKYLVKWATSVVERKGR ERQ+IFED EHSSAY AF+V
Sbjct: 373 THVIHQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRIERQMIFEDSEHSSAY*HAFKV 552
Query: 593 VNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
NPNHLPPLK+LRKLMRIQSLRCNALYNHSATRLSV IHASRSQA+R
Sbjct: 553 DNPNHLPPLKDLRKLMRIQSLRCNALYNHSATRLSVTTIHASRSQAIR 696
Score = 372 bits (806), Expect = e-104
Identities = 176/228 (77%), Positives = 188/228 (82%)
Frame = -3 / -1
Query: 735 LRA*ERDAWIGTTDNLVALWLYNALQRRDWILINFLNSFKGGR*LGLTTLNARQYAEECS 556
L A*ERDA I TD+LVALWL +ALQR DWILINFL SF+GGR* GL+TLNA Q AEECS
Sbjct: 695 LIA*ERDA*IVVTDSLVALWL*SALQRSDWILINFLKSFRGGR*FGLSTLNACQ*AEECS 516
Query: 555 GSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*SNANVILELFDVSLECE 376
SS IICLS+LPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F* V LEL V E E
Sbjct: 515 ESSNIICLSILPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*CITCVTLELVGVLPEYE 336
Query: 375 G**MLLPLLQMKASKSVGSMPPDSSSECISDIVKEVDSTKPVEPDMFSLCMTSIAFLIIL 196
G**MLLPLLQMKASK G MP D+SSE ISDIV +VDSTKPVE +FS+ SIAFLII
Sbjct: 335 G**MLLPLLQMKASKPAGCMPTDTSSEQISDIVSDVDSTKPVEAGVFSV*AASIAFLIIR 156
Query: 195 TRPSLLTESTEITMIFFLPEIGVLLRSSLLPIAGDLGPRVSILDR*FL 52
TR SLLTESTEIT+I FL +IG LL SSLLPIAGDL PRVSILD+*FL
Sbjct: 155 TRSSLLTESTEITIICFLHDIGALLLSSLLPIAGDLAPRVSILDK*FL 12
Score = 309 bits (670), Expect = 3e-085
Identities = 137/228 (60%), Positives = 164/228 (71%)
Frame = +1 / +3
Query: 52 QELPVKDTNSWPKVSCYW*QRGAE*YTYFRKEEYHCYFCRLC*QGRSSQDNQKCY*GHT* 231
QE VKDT+SW K+SCY *QRG E* TY K+ Y+CYFC LC*QGRSS DNQKCY*G +
Sbjct: 12 QESLVKDTDSWCKISCYR*QRGKE*CTYIMKKAYNCYFCGLC*QGRSSPDNQKCY*GCSY 191
Query: 232 RKHVGFNWFCAVNFLNDIRNTFTATIWRHASH*F*CFHLQ*WE*HLLSFTFQRNVKQLQD 411
R+H GFNWF AVN N+IR+ FTA+I RHAS F* FHLQ*WE*HLLSF F+ Q Q
Sbjct: 192 REHAGFNWFRAVNVTNNIRDLFTASICRHASCWF*RFHLQ*WE*HLLSFIFR*YAHQFQG 371
Query: 412 YICIRSKSPVTY*VSLGRRRPKEISCEVGHFSGRKKGKNRETNYF*RSRALFGVLXXXXX 591
Y C SKSP+TY*VSLGRRR K++S E+G+FSGRKKGKN + N *R F +L
Sbjct: 372 YTCNTSKSPITY*VSLGRRRSKKVSSEMGYFSGRKKGKN*KANDI*RF*TFFCLLTCI*S 551
Query: 592 XXXXXXXXFKGVEEVDENPISPLQCII*PQRYQIICSPYPRITLSGSK 735
K +EEVDE+P++PL C +*PQ YQ +C+ Y R++LSG+K
Sbjct: 552 GQSKSSTSSKRLEEVDEDPVAPL*CAL*PQCYQTVCNYYSRVSLSGNK 695
Score = 281 bits (609), Expect = 7e-077
Identities = 138/229 (60%), Positives = 157/229 (68%)
Frame = -1 / -2
Query: 737 TLEPESVMRG*GLQIIW*RCGYIMHCNGEIGFSSTSSTPLKEEDDWD*PL*MQDNTPKSA 558
TL PES R * LQ +W* CGY H NG G SSTSS+ L+E DD D PL*M N K+
Sbjct: 697 TLLPESETRE**LQTVW*HCGYKAHYNGATGSSSTSSSLLEEVDDLDCPL*MHVNRQKNV 518
Query: 557 LDLQK*FVSXXXXXXXXLKWPTSQDISLGLLLPNDTQYVTGDFDLMQM*SWSCLTFLWNV 378
+LQ F LK P S D L LLLPNDTQYV GDFD++ +* W+ + N+
Sbjct: 517 QNLQISFAFQFFPFFLPLK*PISLDTFLDLLLPNDTQYVIGDFDVLHV*PWNWWAYYLNM 338
Query: 377 KDSKCYSHYCR*KHQNQWEACLQIVAVNVFLISLRKLTAQNQLNPTCFLYV*PQ*HF*LS 198
KD+KCYSHYCR*K QNQ +ACLQI+AVN LI L LTA+NQL P C LY PQ*HF*LS
Sbjct: 337 KDNKCYSHYCR*KRQNQQDACLQILAVNKSLILLVTLTARNQLKPACSLYEQPQ*HF*LS 158
Query: 197 *LDLPC*QSLQK*Q*YSSFLK*VYYSAPLCYQ*QETLGQELVSLTGNSC 51
LDLPC*QS QK*Q Y+ F+ *V+YS PLCY *QE L QE VSLT +SC
Sbjct: 157 GLDLPC*QSPQK*QLYAFFMI*VHYSFPLCYL*QEILHQESVSLTSDSC 11
Score = 279 bits (604), Expect = 3e-076
Identities = 147/230 (63%), Positives = 162/230 (70%)
Frame = -2 / -3
Query: 736 P*SLRA*CVDRDYR*SGSAVVI*CIATERLDSHQLPQLL*RRKMIGINHSKCKTIRRRVL 557
P LRA V+ YR SGS VVI I TERLD HQLPQ+ *RR MI I H KC +I RR+
Sbjct: 696 PYCLRARRVNSSYRQSGSTVVIKRITTERLDPHQLPQVF*RR*MIWIVHFKCMSIGRRMF 517
Query: 556 WIFKNNLSLCSSLSFYH*SGPLHKIFP*AFSSPTILNM*LVILI*CKCNLGVV*RFSGM* 377
IFK +L SSLSFYH*S P H I * FSSPTILNM*LVIL+ CNLG R + +*
Sbjct: 516 RIFKYHLPFNSSLSFYH*SSPFH*ILS*TFSSPTILNM*LVILMYYMCNLGTGGRIT*I* 337
Query: 376 RIVNVTPTIADESIKISGKHASR**Q*MYF*YR*GS*QHKTS*TRHVFFMYDLNSISDYP 197
RI+N TPTIADES+K S HA R *Q* *Y * *QH+TS*+R V M LNSISDYP
Sbjct: 336 RIINATPTIADESVKTSRMHAYRY*Q*TNL*YC**R*QHETS*SRRVLCMSSLNSISDYP 157
Query: 196 D*TFLVNRVYRNNNDILPS*NRCITPLLSVTNSRRPWAKS*YP*QVILAM 47
D* FLVNRV+RNNN +L S* RCITP L T SRR KS YP*QVILA+
Sbjct: 156 D*IFLVNRVHRNNNYMLSS*YRCITPFLFATYSRRSCTKSQYP*QVILAL 7
Score = 263 bits (569), Expect(2) = 3e-072
Identities = 132/211 (62%), Positives = 158/211 (74%)
Frame = +3 / +2
Query: 105 VTERSGVIHLFQEGRISLLFL*TLLTRKV*SG*SEMLLRSYIKKTCRVQLVLCCQLP*RY 284
V +R GV+HL+ E I LLFL TLLTRK+*SG*SEMLLR I++T R+QLV CCQ *+Y
Sbjct: 65 VAKRKGVMHLYHEESI*LLFLWTLLTRKI*SG*SEMLLRLLIQRTRRLQLVSCCQRH*QY 244
Query: 285 QKYIHCYYLEACFPLILMLSSAIVGVTFTILHIPEKRQTTPRLHLH*IKITSHILSIVGE 464
Q+++HC YL AC L+L LSSAIVGV F ILHI R PRLH+ IKIT+HILSIVGE
Sbjct: 245 QRFVHC*YL*ACILLVLTLSSAIVGVAFIILHIQVIRPPVPRLHM*YIKITNHILSIVGE 424
Query: 465 EKA*GNIL*SGPLQW*KEREEQRDKLFLKIQSTLRRIVLHLEWLIPIIFLL*RS*GS**E 644
EK * +I *+G LQW*KEREE + K +LKI + L I +HL+W I II+LL*++*GS**
Sbjct: 425 EKV*ESI**NGLLQW*KEREELKGK*YLKILNILLPIDMHLKWTIQIIYLL*KT*GS**G 604
Query: 645 SNLSVAMHYITTALPDYL*SLSTHHALRL*G 737
S+ SV M +ITT LPD L* L T ALR *G
Sbjct: 605 SSRSVVMRFITTVLPDCL*LLFTRLALRQ*G 697
Score = 23.5 bits (45), Expect(2) = 3e-072
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +3 / +2
Query: 51 ARITCQGY 74
ARITCQGY
Sbjct: 11 ARITCQGY 34
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,441,095
Number of Sequences: 8143
Number of extensions: 118088
Number of successful extensions: 878
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 245902276
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)