TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404800.2.1
(1228 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816101.1|CN816101 HRO4517_A05_A09ZS5 Lib AA071E1X Aven... 187 4e-048
gb|CN815034.1|CN815034 HRO4505_B06_C11ZS5 Lib AA071E1X Aven... 180 4e-046
gb|CN814875.1|CN814875 HRO4503_D10_H19ZS5 Lib AA071E1X Aven... 166 9e-042
gb|CN816873.1|CN816873 HRO4525_G05_M09ZS5 Lib AA071E1X Aven... 153 3e-040
gb|CN821053.1|CN821053 HRO4427_A04_B07ZS5 Lib AA069E1X Aven... 153 7e-038
gb|CN815850.1|CN815850 HRO4511_B09_D17ZS5 Lib AA071E1X Aven... 152 1e-037
gb|CN814789.1|CN814789 HRO4502_E05_I10ZS5 Lib AA071E1X Aven... 133 6e-032
gb|CN816425.1|CN816425 HRO4520_F02_L04ZS5 Lib AA071E1X Aven... 113 7e-026
gb|CN814955.1|CN814955 HRO4504_C09_F18ZS5 Lib AA071E1X Aven... 106 6e-024
>gb|CN816101.1|CN816101 HRO4517_A05_A09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_A05_A09, mRNA sequence
Length = 652
Score = 187 bits (402), Expect = 4e-048
Identities = 72/145 (49%), Positives = 101/145 (69%)
Frame = -1 / +3
Query: 610 AFPHVQCSVLDLPHVAANAPANTSVKYIAGDMFESIPPADAVFLKWVLHDWGDADCIKIL 431
A+PH++C+VLDLP V AP + + Y+AGD+F ++PP+ AV LK VLH W D DC+KIL
Sbjct: 18 AYPHLKCTVLDLPKVIEKAPPHDVINYVAGDLFHTVPPSQAVMLKLVLHFWSDEDCVKIL 197
Query: 430 KNCKKAIPSRDAGGKVIIVDMVVGGQSSNIKHKETQVLFDLFIMTINGAERDEHEWKKII 251
C+KAIPSR+ GGKVII+++VV I + + L ++ G +RDE W+++
Sbjct: 198 AQCRKAIPSREEGGKVIIIEIVVEPSLGPIMFEAQLLFDMLMMVNTRGGQRDEKHWRELF 377
Query: 250 SEAGFSDYKIIPVLGVRSIIEVYP* 176
+AGF+DYKI+ LG RS+IEVYP*
Sbjct: 378 MQAGFTDYKIVKKLGARSVIEVYP* 452
>gb|CN815034.1|CN815034 HRO4505_B06_C11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4505_B06_C11, mRNA sequence
Length = 629
Score = 180 bits (388), Expect = 4e-046
Identities = 68/146 (46%), Positives = 99/146 (67%)
Frame = -1 / +1
Query: 619 ISTAFPHVQCSVLDLPHVAANAPANTSVKYIAGDMFESIPPADAVFLKWVLHDWGDADCI 440
I+ A PH++C+VLDLP V P++ V Y+AGD+F S+P + AV LK VLH W D DC+
Sbjct: 43 ITKAHPHIKCTVLDLPKVIEKVPSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDDDCV 222
Query: 439 KILKNCKKAIPSRDAGGKVIIVDMVVGGQSSNIKHKETQVLFDLFIMTINGAERDEHEWK 260
KIL C+ AIPSR+ GGKVII+D++VG I + ++ ++ G +RDE++W+
Sbjct: 223 KILSQCRSAIPSREEGGKVIIIDILVGPSLGPIMFEAQILMDMAMLVNTRGRQRDENDWR 402
Query: 259 KIISEAGFSDYKIIPVLGVRSIIEVY 182
++ +AGFSDYK++ LG R + EVY
Sbjct: 403 QLFMKAGFSDYKVVKKLGARGVFEVY 480
>gb|CN814875.1|CN814875 HRO4503_D10_H19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_D10_H19, mRNA sequence
Length = 846
Score = 166 bits (356), Expect = 9e-042
Identities = 69/94 (73%), Positives = 79/94 (84%)
Frame = -1 / +1
Query: 457 GDADCIKILKNCKKAIPSRDAGGKVIIVDMVVGGQSSNIKHKETQVLFDLFIMTINGAER 278
GD DC+KILK CK+AIPSRDAGGKVIIVDMVVG ++I +ETQV FDLFIM + G ER
Sbjct: 10 GDEDCVKILKKCKEAIPSRDAGGKVIIVDMVVGSGPNDIVTRETQVFFDLFIMYLEGIER 189
Query: 277 DEHEWKKIISEAGFSDYKIIPVLGVRSIIEVYP* 176
+E EWKKI EAGFS+YKII VLGVRS+IE+YP*
Sbjct: 190 EEFEWKKIFMEAGFSEYKIISVLGVRSVIELYP* 291
Score = 101 bits (216), Expect = 2e-022
Identities = 48/96 (50%), Positives = 63/96 (65%)
Frame = +2 / -1
Query: 176 SWVDLDDGSNT*NGYDFVVAKSSFGNYLFPLMFVSLGAIDRHDEEIKQDLSLFVLNV*RL 355
S V+LD+ SN + YDFV+A+ F YL PL F+SL + HD+EIK++L L NV
Sbjct: 291 SRVELDNRSNAQH*YDFVLAEPGFHEYLLPLKFLSLNPLKIHDKEIKENLCLSCHNVIWP 112
Query: 356 ASNNHINDYHLSSCIPGRYSFLTVLEYLDTISIAPV 463
SN+HIN+YHL + I GR SFL +YLDT+ I V
Sbjct: 111 RSNHHINNYHLPTGISGRDSFLAFFQYLDTVFITLV 4
>gb|CN816873.1|CN816873 HRO4525_G05_M09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4525_G05_M09, mRNA sequence
Length = 662
Score = 153 bits (328), Expect(2) = 3e-040
Identities = 58/129 (44%), Positives = 85/129 (65%)
Frame = -1 / +2
Query: 568 VAANAPANTSVKYIAGDMFESIPPADAVFLKWVLHDWGDADCIKILKNCKKAIPSRDAGG 389
V P++ V Y+AGD+F S+P + AV LK VLH W D DC+KIL C+ AIPSR+ GG
Sbjct: 41 VIEKVPSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDDDCVKILSQCRSAIPSREEGG 220
Query: 388 KVIIVDMVVGGQSSNIKHKETQVLFDLFIMTINGAERDEHEWKKIISEAGFSDYKIIPVL 209
KVII+D++VG I + ++ ++ G +RDE++W+++ +AGFSDYK++ L
Sbjct: 221 KVIIIDILVGPSLGPIMFEAQILMDMAMLVNTRGRQRDENDWRQLFMKAGFSDYKVVKKL 400
Query: 208 GVRSIIEVY 182
G R + EVY
Sbjct: 401 GARGVFEVY 427
Score = 27.6 bits (54), Expect(2) = 3e-040
Identities = 8/11 (72%), Positives = 11/11 (100%)
Frame = -1 / +3
Query: 604 PHVQCSVLDLP 572
PH++C+VLDLP
Sbjct: 6 PHIKCTVLDLP 38
>gb|CN821053.1|CN821053 HRO4427_A04_B07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_A04_B07, mRNA
sequence
Length = 644
Score = 153 bits (328), Expect = 7e-038
Identities = 58/124 (46%), Positives = 84/124 (67%)
Frame = -1 / +3
Query: 553 PANTSVKYIAGDMFESIPPADAVFLKWVLHDWGDADCIKILKNCKKAIPSRDAGGKVIIV 374
P++ V Y+AGD+F S+P + AV LK VLH W D DC+KIL C+ AIPSR+ GGKVII+
Sbjct: 24 PSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDEDCVKILSQCRNAIPSREKGGKVIII 203
Query: 373 DMVVGGQSSNIKHKETQVLFDLFIMTINGAERDEHEWKKIISEAGFSDYKIIPVLGVRSI 194
D++VG I + ++ ++ G +RDE++W+++ +AGFSDYKI+ LG R +
Sbjct: 204 DILVGPSLGPIMFEAQILMDMAMLVNTRGRQRDENDWRQLFMKAGFSDYKIVKKLGARGV 383
Query: 193 IEVY 182
EVY
Sbjct: 384 FEVY 395
>gb|CN815850.1|CN815850 HRO4511_B09_D17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_B09_D17, mRNA sequence
Length = 621
Score = 152 bits (326), Expect = 1e-037
Identities = 58/124 (46%), Positives = 84/124 (67%)
Frame = -1 / +2
Query: 553 PANTSVKYIAGDMFESIPPADAVFLKWVLHDWGDADCIKILKNCKKAIPSRDAGGKVIIV 374
P++ V Y+AGD+F S+P + AV LK VLH W D DC+KIL CK AIPSR+ GGKVII+
Sbjct: 2 PSDGIVYYVAGDLFHSVPKSQAVMLKLVLHRWSDEDCVKILSQCKSAIPSREEGGKVIII 181
Query: 373 DMVVGGQSSNIKHKETQVLFDLFIMTINGAERDEHEWKKIISEAGFSDYKIIPVLGVRSI 194
D++VG I + ++ ++ G +RDE++W+++ +AGFSDYKI+ +G R +
Sbjct: 182 DILVGPSLGPIMFEAQVLMDMAMLVNTRGRQRDENDWRQLFMKAGFSDYKIVKKVGARGV 361
Query: 193 IEVY 182
EVY
Sbjct: 362 FEVY 373
>gb|CN814789.1|CN814789 HRO4502_E05_I10ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_E05_I10, mRNA sequence
Length = 596
Score = 133 bits (285), Expect = 6e-032
Identities = 51/109 (46%), Positives = 73/109 (66%)
Frame = -1 / -3
Query: 508 SIPPADAVFLKWVLHDWGDADCIKILKNCKKAIPSRDAGGKVIIVDMVVGGQSSNIKHKE 329
S+P + AV LK VLH W D DC+KIL C+ AIPSR+ GGKVII+D++VG I +
Sbjct: 591 SVPKSQAVLLKLVLHHWSDEDCVKILSQCRNAIPSREKGGKVIIIDILVGPSLGPIMFEA 412
Query: 328 TQVLFDLFIMTINGAERDEHEWKKIISEAGFSDYKIIPVLGVRSIIEVY 182
++ ++ G +RDE++W+++ +AGFSDYKI+ LG R + EVY
Sbjct: 411 QILMDMAMLVNTRGRQRDENDWRQLFMKAGFSDYKIVKKLGARGVFEVY 265
>gb|CN816425.1|CN816425 HRO4520_F02_L04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_F02_L04, mRNA sequence
Length = 538
Score = 113 bits (241), Expect = 7e-026
Identities = 42/93 (45%), Positives = 62/93 (66%)
Frame = -1 / +1
Query: 460 WGDADCIKILKNCKKAIPSRDAGGKVIIVDMVVGGQSSNIKHKETQVLFDLFIMTINGAE 281
W D DC+KIL C+ AIPSR+ GGKVII+D++VG I + ++ ++ G +
Sbjct: 13 WSDEDCVKILSQCRNAIPSREKGGKVIIIDILVGPSLGPIMFEAQILMDMAMLVNTRGRQ 192
Query: 280 RDEHEWKKIISEAGFSDYKIIPVLGVRSIIEVY 182
RDE++W+++ +AGFSDYKI+ LG R + EVY
Sbjct: 193 RDENDWRQLFMKAGFSDYKIVKKLGARGVFEVY 291
>gb|CN814955.1|CN814955 HRO4504_C09_F18ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4504_C09_F18, mRNA sequence
Length = 648
Score = 106 bits (227), Expect = 6e-024
Identities = 41/81 (50%), Positives = 54/81 (66%)
Frame = -1 / +1
Query: 538 VKYIAGDMFESIPPADAVFLKWVLHDWGDADCIKILKNCKKAIPSRDAGGKVIIVDMVVG 359
V Y+AGDMF+ +PPA AV +K VLH W D DC+KIL CKKA+PSR+ GGKVI++D+VV
Sbjct: 16 VNYVAGDMFKLVPPAQAVLVKLVLHHWSDEDCVKILAQCKKAVPSREEGGKVIVIDIVVD 195
Query: 358 GQSSNIKHKETQVLFDLFIMT 296
S E + + + T
Sbjct: 196 SSSGPTHEAELLMDVAMMVFT 258
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,741,714
Number of Sequences: 8143
Number of extensions: 167699
Number of successful extensions: 1033
Number of sequences better than 1.0e-020: 10
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 440146980
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)