TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2306434.2.3
(3914 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Aven... 199 4e-051
gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Aven... 186 2e-047
gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Aven... 164 8e-041
gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Aven... 134 8e-032
>gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_G06_M12, mRNA sequence
Length = 521
Score = 199 bits (428), Expect = 4e-051
Identities = 72/107 (67%), Positives = 90/107 (84%)
Frame = -1 / +2
Query: 743 KWXXXXXXXXXXXXLNFIGVVAGISNAINNGYESWGPLFGKLFFAFWVIVHLYPFLKGLV 564
+W +N +G+VAG+S+A+NNGY SWGPLFGKLFF+FWVIVHLYPFLKGL+
Sbjct: 11 QWTTLLIPPTTLIIINMVGIVAGVSDAVNNGYGSWGPLFGKLFFSFWVIVHLYPFLKGLM 190
Query: 563 GRQNRTPTIVIVWSILLASIFSLLWVRVDPFLAKSNGPLLEECGLDC 423
GRQNRTPTIV++WS+LLASIFSL+WVR+DPF+AKS GP+L+ CG+ C
Sbjct: 191 GRQNRTPTIVVLWSVLLASIFSLVWVRIDPFIAKSKGPILKPCGVQC 331
Score = 133 bits (284), Expect = 3e-031
Identities = 57/86 (66%), Positives = 61/86 (70%)
Frame = +1 / -3
Query: 433 PHSSRSGPLLLARNGSTRTHRSEKIEASRMDQTMTIVGVLFCLPTRPLRNGYRWTITQKA 612
PH R GPL LA GS T EK+EASR DQ+ T+VGV FCLP PLRNG RWT+TQK
Sbjct: 321 PHGLRMGPLDLAMKGSILTQTREKMEASRTDQSTTMVGVRFCLPMSPLRNG*RWTMTQKE 142
Query: 613 KKSFPNRGPHDSYPLLIAFEIPATTP 690
KKSFP RGPHD PLL A E PAT P
Sbjct: 141 KKSFPKRGPHDP*PLLTASETPATMP 64
>gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C09_F17, mRNA
sequence
Length = 676
Score = 186 bits (401), Expect = 2e-047
Identities = 71/92 (77%), Positives = 82/92 (89%)
Frame = -1 / +1
Query: 698 NFIGVVAGISNAINNGYESWGPLFGKLFFAFWVIVHLYPFLKGLVGRQNRTPTIVIVWSI 519
N +GVVAG S AIN+GY+SWGPLFGKLFFAFWVIVHLYPFLKGL+GR NRTPTIVIVW+I
Sbjct: 49 NMVGVVAGTSYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAI 228
Query: 518 LLASIFSLLWVRVDPFLAKSNGPLLEECGLDC 423
LLASIFSLLWVR+DPF + GP ++ CG++C
Sbjct: 229 LLASIFSLLWVRIDPFTTRVAGPNIQTCGINC 324
Score = 134 bits (287), Expect = 1e-031
Identities = 61/93 (65%), Positives = 65/93 (69%)
Frame = +1 / -3
Query: 412 PLQLQSRPHSSRSGPLLLARNGSTRTHRSEKIEASRMDQTMTIVGVLFCLPTRPLRNGYR 591
P Q P GP NGS RTH EKIEA R+ QTMTIVGV F LP RPLRNGY+
Sbjct: 335 PFS*QLIPQVWMFGPATRVVNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK 156
Query: 592 WTITQKAKKSFPNRGPHDSYPLLIAFEIPATTP 690
TITQKAKKSFPN GPHD PLL+A E+PATTP
Sbjct: 155 *TITQKAKKSFPNSGPHDW*PLLMA*EVPATTP 57
>gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_H12_O23, mRNA
sequence
Length = 620
Score = 164 bits (353), Expect = 8e-041
Identities = 61/78 (78%), Positives = 71/78 (91%)
Frame = -1 / +2
Query: 656 NGYESWGPLFGKLFFAFWVIVHLYPFLKGLVGRQNRTPTIVIVWSILLASIFSLLWVRVD 477
+GY+SWGPLFGKLFFAFWVIVHLYPFLKGL+GR NRTPTIVIVW+ILLASIFSLLWVR+D
Sbjct: 2 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILLASIFSLLWVRID 181
Query: 476 PFLAKSNGPLLEECGLDC 423
PF + GP ++ CG++C
Sbjct: 182 PFTTRVAGPNIQTCGINC 235
Score = 112 bits (240), Expect = 3e-025
Identities = 52/82 (63%), Positives = 54/82 (65%)
Frame = +1 / -3
Query: 412 PLQLQSRPHSSRSGPLLLARNGSTRTHRSEKIEASRMDQTMTIVGVLFCLPTRPLRNGYR 591
P Q P GP NGS RTH EKIEA R+ QTMTIVGV F LP RPLRNGY+
Sbjct: 246 PFS*QLIPQVWMFGPATRVVNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK 67
Query: 592 WTITQKAKKSFPNRGPHDSYPL 657
TITQKAKKSFP GPHD PL
Sbjct: 66 *TITQKAKKSFPKSGPHDW*PL 1
>gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_A01_A02, mRNA sequence
Length = 474
Score = 134 bits (288), Expect = 8e-032
Identities = 54/60 (90%), Positives = 58/60 (96%)
Frame = -1 / +3
Query: 602 VIVHLYPFLKGLVGRQNRTPTIVIVWSILLASIFSLLWVRVDPFLAKSNGPLLEECGLDC 423
VIVHLYPFLKGL+GRQNRTPTIVIVWSILLASI SLLWVRV+PFLAK++GP LEECGLDC
Sbjct: 12 VIVHLYPFLKGLLGRQNRTPTIVIVWSILLASIISLLWVRVNPFLAKTDGPTLEECGLDC 191
Score = 114 bits (243), Expect = 1e-025
Identities = 47/60 (78%), Positives = 52/60 (86%)
Frame = +1 / -3
Query: 424 QSRPHSSRSGPLLLARNGSTRTHRSEKIEASRMDQTMTIVGVLFCLPTRPLRNGYRWTIT 603
QS+PHSSR GP +LAR G TRT RSE +EA R+DQTMTIVGVLFCLP+RPLRNG RWTIT
Sbjct: 190 QSKPHSSRVGPSVLARKGLTRTQRSEMMEARRIDQTMTIVGVLFCLPSRPLRNG*RWTIT 11
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 41,276,998
Number of Sequences: 8143
Number of extensions: 607810
Number of successful extensions: 3834
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 47
effective length of database: 1,184,766
effective search space used: 1489250862
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)