TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2276562.2.1
(1286 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN820263.1|CN820263 HRO4417_D05_G09ZS5 Lib AA069E1X Aven... 324 5e-090
gb|CN820072.1|CN820072 HRO4410_G07_M14ZS5 Lib AA069E1X Aven... 110 5e-025
gb|CN818277.1|CN818277 HRO4470_C12_F24ZS5 Lib AA070E1X Aven... 109 9e-025
>gb|CN820263.1|CN820263 HRO4417_D05_G09ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4417_D05_G09, mRNA
sequence
Length = 592
Score = 324 bits (702), Expect(2) = 5e-090
Identities = 137/164 (83%), Positives = 150/164 (91%)
Frame = -3 / +1
Query: 690 AYRYDGPEVNTSLAHSEAKILHEKIHKKAYSDEEIIRILTTRSKAQLLATFNSYKDQFTH 511
AYRY PEVNTS+AHSEAK+LH KI +KAY DEEIIRILTTRSKAQLLATFNSY DQF+H
Sbjct: 4 AYRYFRPEVNTSVAHSEAKLLHGKIQEKAYGDEEIIRILTTRSKAQLLATFNSYNDQFSH 183
Query: 510 AINKDLKADPKDEFLSTLRAIIRCFTCPDRYFEKVIRLALGGMGTDEDDLTRVVTTRAEV 331
I KDLK DPKDEFL+TLRAIIRCF CPDRYFEK+IRLALGG+GTDE+ LTR++TTRAEV
Sbjct: 184 PITKDLKEDPKDEFLATLRAIIRCFACPDRYFEKIIRLALGGVGTDENSLTRIITTRAEV 363
Query: 330 DLKLIKEAYQKRNSVPLERAVAKDTTRDYEDIMLALLGAE*GGV 199
DLKLIKEAY KRNSVPLE+AV+KDTTRDYED+MLALLGAE GV
Sbjct: 364 DLKLIKEAYLKRNSVPLEKAVSKDTTRDYEDMMLALLGAEY*GV 495
Score = 215 bits (465), Expect = 9e-057
Identities = 95/163 (58%), Positives = 123/163 (75%)
Frame = +1 / -2
Query: 199 HASSLRPKEGKHNILIISSCIFGHSTLQRHTVSLLISLLYQLQVDLGASSYDPGEVILIS 378
+AS L ++ KH++ I+S + G+S LQR TVSL + LL QLQV LGAS YDP + +L+
Sbjct: 495 YASVLCSEKCKHHVFIVSGRVLGNSFLQRDTVSLQVGLLDQLQVHLGASGYDPCKGVLVC 316
Query: 379 AHASKSQSNDFLEVSVRAGEAPDDRPQC*KKLILGVSFQILVDCMSELILVAIECCK*LS 558
A SKSQ +D L+VSVRAGEAP DRPQ ++LILG+ Q+L D M+ELI+V +ECCK*LS
Sbjct: 315 ADTSKSQPDDLLKVSVRAGEAPYDRPQGRQELILGILLQVLGDGMTELIIVTVECCK*LS 136
Query: 559 FAPCGENSNDLLITVSLLMDLLMKYFGFRMSQRCVHLWAIIAV 687
FAPC EN ++ L+T+SLL+DL M+ FG R+ CVHLWA IAV
Sbjct: 135 FAPCSENPDNFLVTISLLLDLTME*FGLRVCH*CVHLWAEIAV 7
Score = 179 bits (385), Expect = 1e-045
Identities = 91/163 (55%), Positives = 106/163 (65%)
Frame = -2 / +3
Query: 688 VPL*WPRGEHIFGSF*SQNTS*EDP*EGLQ**GDH*NSHHTEQSSATCNIQ*LQGSVHSC 509
+PL PRGEHI G+ * Q T DP EGL * G++ +SH+ EQSSATCNIQ LQ SV S
Sbjct: 6 IPLFPPRGEHISGTL*GQITPW*DPGEGLW*RGNYQDSHYKEQSSATCNIQQLQ*SVQSS 185
Query: 508 NQQGSES*PQG*VSFNTAGDHPVLHLP*QILRESHSTGSWRHGH**G*PHQGRNYSRRGR 329
+ QG E QG*V + GDH VL LP*QIL E H GSWR H P++ N+SRRG
Sbjct: 186 HHQGPEGGSQG*VPGDPEGDHKVLRLP*QIL*EDHPVGSWRCRHRRELPYKDHNHSRRGG 365
Query: 328 PEADKGGLSEEKQCAAGACCGQRYN*RL*GYYACPPWGGVRRR 200
PEAD+GGL EEKQC AG C Q ++ RL* + AC V RR
Sbjct: 366 PEADQGGLPEEKQCPAGESCFQGHDQRL*RHDACTSRSRVLRR 494
Score = 173 bits (372), Expect = 6e-044
Identities = 92/162 (56%), Positives = 103/162 (63%)
Frame = +3 / -1
Query: 201 RLLTPPQGGQA*YPHNL*LYLWPQHAPAAHCFSSDKPPLSASGRPRRE*LRPW*GHPHQC 380
RL T + QA H+L W Q +PA HCFSS +PP SASG PRRE L *G C
Sbjct: 493 RLSTLLREVQASCLHSLWSCPWKQLSPAGHCFSSGRPP*SASGPPRREWL*SL*GSSRLC 314
Query: 381 PCLQEPVE*LSRSICQGR*STG*SPAVLKETHPWGQLSDPC*LHE*TDPCSY*MLQVAEL 560
LQEP * S+SICQGR ST *SP+ THPW S P * +*TD C+ *MLQVAEL
Sbjct: 313 RHLQEPTG*SSQSICQGRRSTL*SPSGSPGTHPWDPPSGPW*WDD*TDHCNC*MLQVAEL 134
Query: 561 CSVW*EF**SPHHCKPSYGSSHEVFWLQNEPKMCSPLGHHSG 686
CS+ *E ** P H KPS GS H V W Q+ P MCSPLG +SG
Sbjct: 133 CSL**ES**FPRHHKPSPGSYHGVIWPQSVPLMCSPLGGNSG 8
Score = 170 bits (365), Expect = 6e-043
Identities = 91/162 (56%), Positives = 105/162 (64%)
Frame = -1 / +2
Query: 686 TAMMAQR*THLWLILKPKYFMRRSIRRLTVMRRSLEFSPHGAKLSYLQHSIATRISSLMQ 507
TA+ AQR*TH W L+P Y M RS RRL V R+ FS GAKLSYLQHS T ISS++
Sbjct: 8 TAISAQR*THQWHTLRPNYSMVRSRRRLMVTRKLSGFSLQGAKLSYLQHSTVTMISSVIP 187
Query: 506 STRI*KLTPRMSFFQHCGRSSGASPALTDTSRKSFDWLLEAWALMRMTSPGS*LLAPRST 327
S R *+ PRMS ++ GRS GASPALTDT R+S WLLE A R GS* LAPR T
Sbjct: 188 SPRT*RRIPRMSSWRP*GRS*GASPALTDTLRRSSGWLLEVSAQTRTPLQGS*PLAPRWT 367
Query: 326 *S**RRLIRRETVCRWSVLWPKIQLEIMRILCLPSLGRSEEA 201
*S* RR RETV RW L+P+ + E M+ CL +S EA
Sbjct: 368 *S*SRRPT*RETVSRWRKLFPRTRPETMKT*CLHFSEQSTEA 493
Score = 23.1 bits (44), Expect(2) = 5e-090
Identities = 7/19 (36%), Positives = 11/19 (57%)
Frame = -1 / +3
Query: 191 SISSAPTCLMGPKNCIDVV 135
++ P+CLM NC+ V
Sbjct: 510 TLCGGPSCLMAE*NCVKTV 566
>gb|CN820072.1|CN820072 HRO4410_G07_M14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4410_G07_M14, mRNA
sequence
Length = 798
Score = 110 bits (235), Expect = 5e-025
Identities = 52/154 (33%), Positives = 82/154 (53%)
Frame = -3 / +2
Query: 660 TSLAHSEAKILHEKIHKKAYSDEEIIRILTTRSKAQLLATFNSYKDQFTHAINKDLKADP 481
T + H + K ++ IRI T RS A + A ++Y F ++ K +K++
Sbjct: 143 TIVTHDAKDLYKAGEKKFGTDEKTFIRIFTERSWAHMAAVASAYHHMFDRSLVKVVKSET 322
Query: 480 KDEFLSTLRAIIRCFTCPDRYFEKVIRLALGGMGTDEDDLTRVVTTRAEVDLKLIKEAYQ 301
F L I+RC P +YF KV+R ++ G+GTD+ L RVV TR E+D++ IK Y
Sbjct: 323 SGNFEVALVTILRCAENPAKYFAKVLRKSMKGLGTDDKTLVRVVVTRTEIDMQYIKAEYY 502
Query: 300 KRNSVPLERAVAKDTTRDYEDIMLALLGAE*GGV 199
K+ PL A+ +T+ Y +L+L+G+ * V
Sbjct: 503 KKYKKPLADAIHSETSGGYRTFLLSLVGSH*AKV 604
>gb|CN818277.1|CN818277 HRO4470_C12_F24ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4470_C12_F24, mRNA sequence
Length = 620
Score = 109 bits (233), Expect = 9e-025
Identities = 49/133 (36%), Positives = 77/133 (57%)
Frame = -3 / +1
Query: 597 DEEIIRILTTRSKAQLLATFNSYKDQFTHAINKDLKADPKDEFLSTLRAIIRCFTCPDRY 418
++ IRI T RS A + A ++Y F ++ K +K++ F L I+RC P +Y
Sbjct: 100 EKTFIRIFTERSWAHMAAVASAYHHMFDRSLVKVVKSETSGNFEVALVTILRCAENPAKY 279
Query: 417 FEKVIRLALGGMGTDEDDLTRVVTTRAEVDLKLIKEAYQKRNSVPLERAVAKDTTRDYED 238
F KV+R ++ G+GTD+ L RVV TR E+D++ IK Y K+ PL A+ +T+ Y
Sbjct: 280 FAKVLRKSMKGLGTDDKTLVRVVVTRTEIDMQYIKAEYYKKYKKPLADAIHSETSGGYRT 459
Query: 237 IMLALLGAE*GGV 199
+L+L+G+ * V
Sbjct: 460 FLLSLVGSH*AKV 498
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,348,041
Number of Sequences: 8143
Number of extensions: 174186
Number of successful extensions: 879
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 464330880
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)