TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2276343.2.2
(1340 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN820987.1|CN820987 HRO4422_C03_E06ZS5 Lib AA069E1X Aven... 345 3e-098
gb|CN820362.1|CN820362 HRO4418_E04_I08ZS5 Lib AA069E1X Aven... 158 2e-039
>gb|CN820987.1|CN820987 HRO4422_C03_E06ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4422_C03_E06, mRNA
sequence
Length = 649
Score = 345 bits (747), Expect(2) = 3e-098
Identities = 136/177 (76%), Positives = 157/177 (88%)
Frame = +1 / +2
Query: 436 ECSWAPIFVRQSNFKLPADPSTPIIMIGPGTGLAPFRGFLQERLALKQSGAELGTSILFF 615
E +WAPIFVRQSNFKLP DPS PIIMIGPGTGLAPFRGFLQERL K+SGAE+G S+ FF
Sbjct: 56 EDNWAPIFVRQSNFKLPTDPSVPIIMIGPGTGLAPFRGFLQERLVQKESGAEMGRSVFFF 235
Query: 616 GCRNRNMDYIYEDELQTFLEEGALSELIVAFSREGPTKEYVQHKMVEKATDIWNIISNGG 795
GCRN MD+IYEDEL FLE+GALSEL++AFSR+ PTKEYVQHKM +KA++IW++IS GG
Sbjct: 236 GCRNSKMDFIYEDELNNFLEQGALSELVLAFSRQDPTKEYVQHKMGQKASEIWDVISQGG 415
Query: 796 YLYVCGDAKGMARDVHKMLHTIVQEQGSLDNSKTESYVKSLQMEGRYLRDVW*HITS 966
Y+YVCGDAKGMA+DVH++LHTIVQEQGSLD+SK E +VK+LQ EGRYLRDVW* S
Sbjct: 416 YIYVCGDAKGMAKDVHRVLHTIVQEQGSLDSSKAEGFVKNLQTEGRYLRDVW*EAIS 586
Score = 264 bits (570), Expect = 3e-071
Identities = 117/178 (65%), Positives = 134/178 (75%)
Frame = -1 / -2
Query: 968 FEVMCHHTSRRYLPSICRLFT*LSVLELSKDPCSWTIV*SILCTSLAIPLASPQTYK*PP 789
FE+ +HTS RYLPS+ R FT S ELS DPCS T+V LCTSLA+PLASPQT *PP
Sbjct: 588 FEIASYHTSLRYLPSVWRFFTKPSAFELSSDPCS*TMVWRTLCTSLAMPLASPQT*M*PP 409
Query: 788 FEMMFQISVAFSTILCCTYSFVGPSRENATISSESAPSSRKVCNSSSYM*SILRFLHPKK 609
+E+ QIS AF ILCCTYS VG RE A +S++AP SRK+ +SSS M SIL FLHPKK
Sbjct: 408 WEITSQISDAFCPILCCTYSLVGS*REKARTNSDNAPCSRKLFSSSS*MKSILLFLHPKK 229
Query: 608 RIEVPSSAPDCFSANLSCKKPLKGASPVPGPIMIIGVDGSAGNLKFDCLTNMGAQLHS 435
+ + P SAPD F NLSCKKP KGA PVPGPIMIIG +GS G+LKFDCLT +GAQL S
Sbjct: 228 KTDRPISAPDSFCTNLSCKKPRKGARPVPGPIMIIGTEGSVGSLKFDCLTKIGAQLSS 55
Score = 157 bits (338), Expect = 3e-039
Identities = 76/172 (44%), Positives = 106/172 (61%)
Frame = -3 / -1
Query: 951 PYITEVPSFHLQALYIALGFGVIQRSLLLDYCMKHFVYISSHSLGITANI*VTTI*DDVP 772
P+I ++ +F L+ L+ ALG ++ SLLL+ +++ ++I H+ GITAN+ VT + D+VP
Sbjct: 571 PHIPQISTFRLEVLHKALGL*TVK*SLLLNNGVENSMHIFGHAFGITANVNVTALGDNVP 392
Query: 771 NICGLFHHLMLHIFFRWPFPRECNN*LRKRPLLKESLQLIFIYVVHITVPASKEKD*SAQ 592
N+ H +LHIF RE N LR+ LLKE +QLI + +H V ASKE+D* A
Sbjct: 391 NLRCFLPHFVLHIFLSRVLTREGENQLRQCSLLKEVVQLILVDEIHFAVSASKEED*PAH 212
Query: 591 FCSRLFQC*PFLQEASKRSQPCPRTNHDNRSGWVSR*LEI*LPHKYGSPAAF 436
SR F P LQEA++R + C NHDNR +S E+*LPHK SP F
Sbjct: 211 LSSRFFLHQPLLQEAAERCKTCSWANHDNRH*RISGKFEV*LPHKNWSPVIF 56
Score = 77.6 bits (163), Expect(2) = 1e-028
Identities = 32/68 (47%), Positives = 44/68 (64%)
Frame = +3 / +1
Query: 444 LGSHICEAVKFQVTC*PIHSDYHDWSWDRAGSF*RLLAGKVSTETIWSRTGHFNPFLWMQ 623
LGS+ CEA+K Q + * +DYHDW +R+ +F RLLAG+V + IWS G + LWMQ
Sbjct: 64 LGSNFCEAIKLQTSH*SFSADYHDWPRNRSCTFPRLLAGEVGAKRIWS*DGPVSLLLWMQ 243
Query: 624 EP*YGLHI 647
+ G H+
Sbjct: 244 KQQNGFHL 267
Score = 64.8 bits (135), Expect(2) = 1e-028
Identities = 30/71 (42%), Positives = 41/71 (57%)
Frame = +2 / +3
Query: 716 KGQRKNMCSIRWWKRPQIFGTSSQMVVTYMFAVMPREWLEMYTKCFIQ*SKSRDLWITPK 895
K + +NMCS +W K+ FGT S VT+ FAVMP+ W +M + +SRD +
Sbjct: 336 KTRLRNMCSTKWGKKHLRFGTLSPRAVTFTFAVMPKAWPKMCIEFSTPLFRSRDHLTVQR 515
Query: 896 PRAM*RACRWK 928
PRA+*R R K
Sbjct: 516 PRAL*RTSRRK 548
Score = 63.8 bits (133), Expect(2) = 6e-023
Identities = 36/77 (46%), Positives = 44/77 (57%)
Frame = +2 / +3
Query: 428 IAKNAAGLPYL*GSQISSYLLTHPLRLS*LVLGQGWLLLEASCRKG*H*NNLEQNWALQS 607
I + GL +L*G+Q S++ L RLS*L Q L ASCR+G NLE WA QS
Sbjct: 48 IHRKITGLQFL*GNQTSNFPLILQCRLS*LAQEQVLHLSAASCRRGWCKKNLELRWAGQS 227
Query: 608 FSLDAGTVIWTTYMKMS 658
SLDA T W + +MS
Sbjct: 228 SSLDAETAKWISSTRMS 278
Score = 59.3 bits (123), Expect(2) = 6e-023
Identities = 34/88 (38%), Positives = 49/88 (55%)
Frame = +3 / +1
Query: 705 ILSGRANERICAA*DGGKGHRYLEHHLKWWLLICLR*CQGNG*RCTQNASYNSPRAGIFG 884
+LS R + ICAA +G K L +L L + LR*CQ +G RC ++ ++ AGI
Sbjct: 325 LLSSRPD*GICAAQNGAKSI*DLGRYLPGRLHLRLR*CQRHGQRCA*SSPHHCSGAGIT* 504
Query: 885 *LQNRELCKEPADGRKVPP*CMVTHHLK 968
+ R LC+EP DGR + C+V L+
Sbjct: 505 QFKGRGLCEEPPDGR*ISEGCVVRGDLE 588
Score = 29.9 bits (59), Expect(2) = 3e-098
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +1 / +2
Query: 382 QGVCSTWMKNTIPLE 426
+GVCSTW+KN P E
Sbjct: 14 KGVCSTWIKNANPSE 58
>gb|CN820362.1|CN820362 HRO4418_E04_I08ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4418_E04_I08, mRNA
sequence
Length = 519
Score = 158 bits (340), Expect = 2e-039
Identities = 60/82 (73%), Positives = 73/82 (89%)
Frame = +1 / +1
Query: 724 TKEYVQHKMVEKATDIWNIISNGGYLYVCGDAKGMARDVHKMLHTIVQEQGSLDNSKTES 903
TKEYVQHKM + A++IW++IS GGY+YVCGDAKGMA+DVH++LHTIVQEQGSLD++K E
Sbjct: 1 TKEYVQHKMAQNASEIWDVISQGGYIYVCGDAKGMAKDVHRVLHTIVQEQGSLDSTKAEG 180
Query: 904 YVKSLQMEGRYLRDVW*HITSN 969
+VK+LQ EGRYLRDVW* SN
Sbjct: 181 FVKNLQTEGRYLRDVW*EAISN 246
Score = 122 bits (261), Expect = 1e-028
Identities = 52/83 (62%), Positives = 61/83 (73%)
Frame = -1 / -2
Query: 977 YGLFEVMCHHTSRRYLPSICRLFT*LSVLELSKDPCSWTIV*SILCTSLAIPLASPQTYK 798
+ +FE+ +HTS RYLPS+ R FT S LS DPCSWT+V LCTSLA+PLASPQT
Sbjct: 254 FRVFEIASYHTSLRYLPSVWRFFTKPSAFVLSSDPCSWTMVWRTLCTSLAMPLASPQT*M 75
Query: 797 *PPFEMMFQISVAFSTILCCTYS 729
*PP+E+ QIS AF ILCCTYS
Sbjct: 74 *PPWEITSQISDAFCAILCCTYS 6
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 16,354,033
Number of Sequences: 8143
Number of extensions: 258195
Number of successful extensions: 1178
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 486096390
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)