TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2253513.2.1
(746 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Aven... 237 1e-063
gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Aven... 226 2e-060
gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Aven... 222 6e-059
gb|CN814825.1|CN814825 HRO4502_H06_O12ZS5 Lib AA071E1X Aven... 79 7e-022
gb|CN815983.1|CN815983 HRO4515_F02_L03ZS5 Lib AA071E1X Aven... 79 1e-021
>gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_D01_H01, mRNA sequence
Length = 653
Score = 237 bits (513), Expect = 1e-063
Identities = 92/97 (94%), Positives = 94/97 (96%)
Frame = +3 / +1
Query: 90 QNQNENRTLWKLGTLPPGLITFYSTTKPLEKSWHVLGLGYNPSISMEEIRNAAVVHFNGN 269
Q QNENR LWKLGTLPPGLITFYSTTKPL+KSWHVLGLGYNPSISMEEIRNAAVVHFNGN
Sbjct: 331 QTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGN 510
Query: 270 MKPWLDIGMNQFRHLWTKYVDYDDSYIRQCNFAPP*K 380
MKPWLDIGMNQFRHLWTKYVDY DS+IRQCNFAPP*K
Sbjct: 511 MKPWLDIGMNQFRHLWTKYVDYGDSFIRQCNFAPP*K 621
Score = 200 bits (431), Expect = 2e-052
Identities = 83/97 (85%), Positives = 87/97 (89%)
Frame = -2 / -1
Query: 379 FYGGAKLH*RIYESS*STYFVQRCLNWFIPMSSQGFMLPLKCTTAAFLISSMLMLGLYPS 200
FYGGAKLH R+ ES *STYFVQRCLNWFIP+SSQGFMLPLKCTTAAFLISSMLMLGLYP
Sbjct: 620 FYGGAKLHWRMNESP*STYFVQRCLNWFIPISSQGFMLPLKCTTAAFLISSMLMLGLYPR 441
Query: 199 PRTCQDFSRGFVVE*KVIKPGGSVPSFHNVLFSF*FC 89
PRTCQD SRGFVVE* VI PGG+VP+FHN FSF* C
Sbjct: 440 PRTCQDLSRGFVVE*NVINPGGNVPNFHNNRFSF*VC 330
Score = 171 bits (367), Expect = 2e-043
Identities = 71/98 (72%), Positives = 81/98 (82%)
Frame = +2 / +3
Query: 89 AESE*EQDIMEARNAAPWLDHFLLDNKTSGEILACSWTWIQS*HQHGGD*KCCCCAFQRQ 268
A+SE*E IME RN APW++H LLD+K SG+ILACSW+ + S HQHGGD K CCCAFQ
Sbjct: 330 ADSE*EPVIMEVRNIAPWVNHILLDHKASGQILACSWSRV*SKHQHGGDQKRCCCAFQW* 509
Query: 269 HEALAGHRYEPVQAPLDKVCGL**LIYSSMQFCTAIEG 382
HEALAG+ YEPVQAPLDKVCGL LI+S +QFCTAIEG
Sbjct: 510 HEALAGYWYEPVQAPLDKVCGLWRLIHSPVQFCTAIEG 623
Score = 166 bits (358), Expect = 3e-042
Identities = 74/107 (69%), Positives = 80/107 (74%)
Frame = +1 / +2
Query: 61 RNVLSSTIIGRIRMRTGHYGS*ERCPLA*SLSTRQQNLWRNLGMFLDLDTILASAWRRLE 240
R + SS + GR+RMRTG+YGS*E CPL *S STR Q+LW N GMFL I ASAWRR E
Sbjct: 302 RKIYSSHVSGRLRMRTGYYGS*EHCPLG*SHSTRPQSLWTNPGMFLVSGIIQASAWRRSE 481
Query: 241 MLLLCISTAT*SPGWTSV*TSSGTFGQSMWTMMTHIFVNAILHRHRR 381
LLLCIS T*S GW V*TSSGT GQSMW M TH F +AILHRHRR
Sbjct: 482 TLLLCISMVT*SLGWILV*TSSGTSGQSMWIMATHSFASAILHRHRR 622
Score = 150 bits (322), Expect = 3e-037
Identities = 67/99 (67%), Positives = 78/99 (78%)
Frame = -3 / -2
Query: 378 SMAVQNCIDEYMSHHSPHTLSKGA*TGSYRCPARASCCR*NAQQQHF*SPPC*C*DCIQV 199
SMAVQNC E MS H+PHTLS+GA*TGSY+ PA+ASC *NAQQQ F*SPPC*C D +
Sbjct: 619 SMAVQNCTGE*MSRHNPHTLSRGA*TGSYQYPAKASCYH*NAQQQRF*SPPC*CLDYTRD 440
Query: 198 QEHAKISPEVLLSSRK*SSQGAAFLASIMSCSHSDSANN 82
QEHA+I PE L SSR * +QGA FL SI++ SHS+SA +
Sbjct: 439 QEHARICPEALWSSRM*LTQGAMFLTSIITGSHSESARH 323
Score = 148 bits (318), Expect = 9e-037
Identities = 60/86 (69%), Positives = 72/86 (83%)
Frame = -1 / -3
Query: 383 NLLWRCKIALTNI*VIIVHILCPKVPELVHTDVQPGLHVAVEMHNSSISNLLHADARIVS 204
+LLWRCKIAL N *V I+HILCP+VPELVHT++QP LHV +EMHNSS+S+LLHADA I+
Sbjct: 624 SLLWRCKIALANE*VAIIHILCPEVPELVHTNIQPRLHVTIEMHNSSVSDLLHADAWIIP 445
Query: 203 KSKNMPRFLQRFCCRVESDQARGQRS 126
++KNMP F+QR C RVE D RGQ S
Sbjct: 444 ETKNMPGFVQRLCGRVECD*PRGQCS 367
>gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4423_D02_H03, mRNA
sequence
Length = 733
Score = 226 bits (489), Expect = 2e-060
Identities = 81/122 (66%), Positives = 94/122 (77%)
Frame = +3 / +1
Query: 3 NACGWAYGMNFFDLDSWRREKCTEQYHYWQNQNENRTLWKLGTLPPGLITFYSTTKPLEK 182
NACGWAYGMN FDL W+ + T YH WQN NE+R LWKLGTLPPGL+TFY T PL+K
Sbjct: 196 NACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDK 375
Query: 183 SWHVLGLGYNPSISMEEIRNAAVVHFNGNMKPWLDIGMNQFRHLWTKYVDYDDSYIRQCN 362
SWHVLGLGYNPSI EI NAAV H+NGNMKPWL++ M ++R WT+Y+ YD YIR CN
Sbjct: 376 SWHVLGLGYNPSIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCN 555
Query: 363 FA 368
+
Sbjct: 556 LS 561
Score = 116 bits (247), Expect = 6e-027
Identities = 63/121 (52%), Positives = 68/121 (56%)
Frame = -2 / -3
Query: 364 KLH*RIYESS*STYFVQRCLNWFIPMSSQGFMLPLKCTTAAFLISSMLMLGLYPSPRTCQ 185
KLH RI S Y VQ I S+ GF+ PL TAA IS MLGLYP+PRTC
Sbjct: 557 KLHPRI*G*SYFIYLVQYGRYLVIASSNHGFIFPL**ATAALSISERSMLGLYPNPRTCH 378
Query: 184 DFSRGFVVE*KVIKPGGSVPSFHNVLFSF*FCQ*WYCSVHFSLLQESRSKKFIPYAHPQA 5
D SRG V * V++PGGSVPSF L SF FC W V RSK FIP A PQA
Sbjct: 377 DLSRGCVSL*NVMRPGGSVPSFQRTLSSFMFCHLW*IPVISLFFHCFRSKMFIP*AQPQA 198
Query: 4 L 2
L
Sbjct: 197 L 195
Score = 96.4 bits (204), Expect = 5e-021
Identities = 47/120 (39%), Positives = 70/120 (58%)
Frame = +2 / +3
Query: 2 *CLWMGIRNEFLRS*FLEEGEMY*AVPLLAESE*EQDIMEARNAAPWLDHFLLDNKTSGE 181
*CLW+G+ +E L S +EE Y +P +A+ E* Q +EAR+ + W L + +
Sbjct: 195 *CLWLGLWDEHL*SKAVEEQRYYRDLPQVAKHE*RQGSLEARDTSTWPHDVLQADTSP** 374
Query: 182 ILACSWTWIQS*HQHGGD*KCCCCAFQRQHEALAGHRYEPVQAPLDKVCGL**LIYSSMQ 361
++AC W IQ H+ GD +CCCC+ Q ++EA+ G + V A LDKV +* +Y MQ
Sbjct: 375 VVACPWIRIQPKHRPLGDRQCCCCSLQWEYEAMVGAGNDQVSAILDKVYKV*SSLYPRMQ 554
>gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_A06_B12, mRNA sequence
Length = 570
Score = 222 bits (479), Expect = 6e-059
Identities = 77/122 (63%), Positives = 96/122 (78%)
Frame = +3 / +1
Query: 3 NACGWAYGMNFFDLDSWRREKCTEQYHYWQNQNENRTLWKLGTLPPGLITFYSTTKPLEK 182
NACGWAYGMN FDL+ W+++ T YH WQ NENR LWKLGTLPPGL+TFY T PL+K
Sbjct: 85 NACGWAYGMNMFDLEEWKKKDITGIYHRWQTLNENRLLWKLGTLPPGLMTFYKLTHPLDK 264
Query: 183 SWHVLGLGYNPSISMEEIRNAAVVHFNGNMKPWLDIGMNQFRHLWTKYVDYDDSYIRQCN 362
SWHVLGLGYNP++ EI +AAV+H+NGNMKPWL+I M ++R WTKY++Y+ SY+ C
Sbjct: 265 SWHVLGLGYNPTVEHSEIDSAAVIHYNGNMKPWLEIAMTKYRPYWTKYINYEHSYVHGCK 444
Query: 363 FA 368
+
Sbjct: 445 IS 450
Score = 119 bits (255), Expect = 4e-028
Identities = 61/114 (53%), Positives = 67/114 (58%)
Frame = -2 / -2
Query: 343 ESS*STYFVQRCLNWFIPMSSQGFMLPLKCTTAAFLISSMLMLGLYPSPRTCQDFSRGFV 164
E S* YFVQ + I +SS GFM PL TAA IS +GLYP+P TC D S G V
Sbjct: 425 ECS*LMYFVQ*GRYFVIAISSHGFMFPL*WMTAALSISECSTVGLYPNPSTCHDLSSGCV 246
Query: 163 VE*KVIKPGGSVPSFHNVLFSF*FCQ*WYCSVHFSLLQESRSKKFIPYAHPQAL 2
*KVI+PGGSVPSFH+ LFSF C W V SRS FIP A P AL
Sbjct: 245 SL*KVIRPGGSVPSFHSSLFSFRVCHLW*IPVMSFFFHSSRSNIFIP*AQPHAL 84
>gb|CN814825.1|CN814825 HRO4502_H06_O12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_H06_O12, mRNA sequence
Length = 837
Score = 79.0 bits (166), Expect(2) = 7e-022
Identities = 27/76 (35%), Positives = 50/76 (65%)
Frame = +3 / +1
Query: 144 LITFYSTTKPLEKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMKPWLDIGMNQFRHLWTK 323
L++F PL++ + GLGY+ I E R++A +H+NGNMKPWL++G+ +++ W +
Sbjct: 322 LLSFQHLIYPLDERLTLSGLGYDYGIDPEVARSSASLHYNGNMKPWLELGILEYKKYWRR 501
Query: 324 YVDYDDSYIRQCNFAP 371
++ +D ++ +CN P
Sbjct: 502 FLTREDRFMDECNANP 549
Score = 39.6 bits (80), Expect(2) = 7e-022
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3 / +1
Query: 6 ACGWAYGMNFFDLDSWRREKCTEQY 80
+C W G+N +LD WR+ K TE Y
Sbjct: 178 SCAWMSGVNVINLDKWRKHKVTENY 252
>gb|CN815983.1|CN815983 HRO4515_F02_L03ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_F02_L03, mRNA sequence
Length = 668
Score = 79.0 bits (166), Expect(2) = 1e-021
Identities = 27/76 (35%), Positives = 50/76 (65%)
Frame = +3 / +1
Query: 144 LITFYSTTKPLEKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMKPWLDIGMNQFRHLWTK 323
L++F PL++ + GLGY+ I E R++A +H+NGNMKPWL++G+ +++ W +
Sbjct: 385 LLSFQHLIYPLDERLILSGLGYDYGIDPEVARSSASLHYNGNMKPWLELGILEYKKYWRR 564
Query: 324 YVDYDDSYIRQCNFAP 371
++ +D ++ +CN P
Sbjct: 565 FLTREDRFMDECNVNP 612
Score = 38.6 bits (78), Expect(2) = 1e-021
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +3 / +1
Query: 6 ACGWAYGMNFFDLDSWRREKCTEQY 80
+C W G+N +LD W++ K TE+Y
Sbjct: 241 SCAWKSGVNVINLDKWKKHKVTEKY 315
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,116,810
Number of Sequences: 8143
Number of extensions: 134970
Number of successful extensions: 1020
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 249554290
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)