TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192958.2.3
(1681 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN180781.1|CN180781 As03_isu1961_C ISU cDNA from oat Ave... 595 e-171
>gb|CN180781.1|CN180781 As03_isu1961_C ISU cDNA from oat Avena sativa cDNA clone
As03_isu1961, mRNA sequence
Length = 1004
Score = 595 bits (1294), Expect = e-171
Identities = 237/274 (86%), Positives = 260/274 (94%)
Frame = -3 / -1
Query: 1238 DLYNGTSKKLSLSRSVLCSKCNGKGSKSGASSRCAGCQGSGFKVQIRQLGPGMIQQMQHP 1059
+LYNGTSKKLSLSR+VLCSKCNGKGSKSGAS +CAGCQG+G+KVQIRQLGPGMIQQMQ P
Sbjct: 1004 ELYNGTSKKLSLSRNVLCSKCNGKGSKSGASMKCAGCQGAGYKVQIRQLGPGMIQQMQQP 825
Query: 1058 CNECKGSGETISDKDRCPQCKGDKVVQEKKVLEVFVEKGMQNGQKITFPGEADEAPDTVT 879
CNEC+G+GETISDKDRC QCKG+KVVQEKKVLEV VEKGMQ+ QKITFPGEADEAPDTVT
Sbjct: 824 CNECRGTGETISDKDRCGQCKGEKVVQEKKVLEVVVEKGMQHSQKITFPGEADEAPDTVT 645
Query: 878 GDIIFVLQQKEHPKFKRKGDDLFYEHTLTLTESLCGFQFVVTHLDNRQLLIKSNPGEVVK 699
GDIIFVLQQKEHPKFKRKGDDLFYEHTLTLTE+LCGFQ+V+THLD RQLLIKSNPGEVVK
Sbjct: 644 GDIIFVLQQKEHPKFKRKGDDLFYEHTLTLTEALCGFQYVLTHLDGRQLLIKSNPGEVVK 465
Query: 698 PDSFKAINDEGMPMYQRPFMKGKLYIHFSVEFPDSLSPEQCKALEAVLPPKPVSQYTDME 519
PDSFKAINDEGMPMYQRPFMKGKLYIHF+V+FPDSL+P+QCKALE VLPPKPVSQYTDME
Sbjct: 464 PDSFKAINDEGMPMYQRPFMKGKLYIHFTVDFPDSLNPDQCKALETVLPPKPVSQYTDME 285
Query: 518 LDECEETMPYDVNIEAEMRRRQQQHQEAYDEDED 417
LDECEETM YD++IE EMRRRQQQ + ++++
Sbjct: 284 LDECEETMAYDIDIEEEMRRRQQQQAQEAYDEDE 183
Score = 477 bits (1035), Expect(2) = e-139
Identities = 200/259 (77%), Positives = 208/259 (80%)
Frame = +1 / +1
Query: 457 RLRISASMFTXXXXXXXXXXXXXXVYCDTGLGGSTASRALHCSGLSESGNSTEKWMYSLP 636
RL IS+SM VYCDTGLGG T SRALH SG SESG ST KWM SLP
Sbjct: 223 RLLISSSMSMSYAIVSSHSSSSMSVYCDTGLGGRTVSRALHWSGFSESGKSTVKWM*SLP 402
Query: 637 FMNGLWYMGMPSSLIALKESGFTTSPGFDLISSCLLSK*VTTNWKPHRDSVKVRVCS*KR 816
FMNGLWYMGMPSSLIAL ESG TTSPG DL+SSCL SK*V T WKPHR SV+VRVCS*KR
Sbjct: 403 FMNGLWYMGMPSSLIALNESGLTTSPGLDLMSSCLPSK*VRTYWKPHRASVRVRVCS*KR 582
Query: 817 SSPFLLNLGCSFCWRTKMISPVTVSGASSASPGNVIFCPFCMPFSTNTSRTFFSCTTLSP 996
SSPF LNLGCSFC RTK++SPVTVSGASSASPG VIF C+PFST TSRTFFSCTT SP
Sbjct: 583 SSPFRLNLGCSFCCRTKIMSPVTVSGASSASPGKVIFWLCCIPFSTTTSRTFFSCTTFSP 762
Query: 997 LHCGHLSLSLIVSPEPLHSLQGCCICWIIPGPNCLIWTLKPEPWQPAHLDEAPDFEPLPL 1176
LHC HLSLSL+VSP PLHSLQGCCIC IIPGPN IWTL P PWQPAH EAPDFEPLPL
Sbjct: 763 LHCPHLSLSLMVSPVPLHSLQGCCIC*IIPGPNWRIWTL*PAPWQPAHFIEAPDFEPLPL 942
Query: 1177 HLEQRTLRDRESFFDVPLY 1233
H EQ TLR+RESF DVPLY
Sbjct: 943 HFEQSTLRERESFLDVPLY 999
Score = 285 bits (616), Expect(3) = 8e-091
Identities = 135/215 (62%), Positives = 150/215 (69%)
Frame = -2 / -3
Query: 1233 VQWHIKEALSVPQCPLLQVQW*GFKVWSFIEVCWLPRFWL*GPNQAVGAWNDPANAASLQ 1054
VQWHI EA S QC LL+VQW G KVW F EVC LP L*GPN VG ND ANAA+LQ
Sbjct: 999 VQWHI*EAFSFTQCALLKVQWQGLKVWGFDEVCRLPGSRL*GPNTPVGTRNDSANAATLQ 820
Query: 1053 RVQGFWRDNQRQG*MPTVQG**SCAGEEGS*SVCGERHAEWAEDHIPW*S**SA*HCHWR 874
VQG W D+QRQG*M VQG CA EEGS E +A EDH+PW *SA*+ +W
Sbjct: 819 *VQGNW*DHQRQG*MWAVQGREGCAREEGSGGSGREGYAAQPEDHLPWRGR*SA*YRYWG 640
Query: 873 YHLCSPAEGASQVQEKG**PLLRAHPDLD*ISVWLPVCCDSLG*QAAADQIKSGRSCEA* 694
++L AEGASQVQ +G PLL AH D * VWLP+C S G QAAA Q++S SC A*
Sbjct: 639 HNLRPTAEGASQVQAEGRRPLL*AHSDPH*SPVWLPICSYSFGRQAAAHQVQSWGSC*A* 460
Query: 693 FFQGDQRRRHAHVPEAVHEGQAVHPLLGGVPGLAE 589
QGDQRR HAHVPEAVHEGQA+HPL GG+PGLAE
Sbjct: 459 LVQGDQRRGHAHVPEAVHEGQALHPLHGGLPGLAE 355
Score = 193 bits (415), Expect(3) = 3e-088
Identities = 83/115 (72%), Positives = 97/115 (84%)
Frame = +3 / +3
Query: 477 DVHVIGHRLLALVQLHVGVLRHRLGREHSLQGLALLRAQRVRELHREVDVQLALHERPLV 656
DV+VI H LL L+ LHV VLRH LGR+ SL GLAL+R QRVRE+HREVDV+LALHERPLV
Sbjct: 243 DVNVIRHSLLTLI*LHVRVLRHGLGRQDSL*GLALVRVQRVREVHREVDVELALHERPLV 422
Query: 657 HGHAFVVDRLERIRLHNFARI*FDQQLPVIQVSHNKLEATQRFSQGQGVLVEEVI 821
HGHA VVDRLER+RL+NF RI D+QLP +Q+S N LEATQ FS+GQ VL+EEV+
Sbjct: 423 HGHALVVDRLERVRLNNFPRIGLDEQLPAVQMSKNILEATQGFSEGQSVLIEEVV 587
Score = 187 bits (403), Expect(3) = 1e-059
Identities = 83/127 (65%), Positives = 90/127 (70%)
Frame = +2 / +2
Query: 488 HRASSPRTRPAPCRCTATPAWAGAQPPGPCTAPGSASPGTPPRSGCTACPS*TASGTWAC 667
H SP T APC CTAT AWA Q GPCT PGSASPG+PP SGC ACPS*TASGTWAC
Sbjct: 254 HTP*SPHTHLAPCPCTATRAWAAGQSLGPCTGPGSASPGSPP*SGCRACPS*TASGTWAC 433
Query: 668 LRR*SP*KNQASQLRPDLI*SAAACYPSESQQTGSHTEIQSRSGCARRRGHHPFS*TWDA 847
RR*SP* +QA QL D * AAAC P+E + GSHT +Q S CA RRG P +*TWDA
Sbjct: 434 PRR*SP*TSQA*QLPQDWT**AAACRPNE*EHIGSHTGLQ*GSECAHRRGRRPSA*TWDA 613
Query: 848 PSAGEQR 868
PSA +R
Sbjct: 614 PSAVGRR 634
Score = 138 bits (296), Expect(3) = 3e-088
Identities = 64/107 (59%), Positives = 70/107 (65%)
Frame = +2 / +2
Query: 914 GM*SSAHSACLSPQTLQEPSSPAQLYHPCTVGIYPCR*LSLQNPCTRCKDAAFAGSFQAP 1093
G *SS +A S EPSS AQ PCT IYPCR* S Q PCT CK AAFA SF P
Sbjct: 680 GR*SSGCAAYPSRPLPPEPSSLAQPSRPCTAHIYPCR*WSHQFPCTHCKVAAFAESFLVP 859
Query: 1094 TA*FGP*SQNLGNQHTSMKLQTLNPYHCTWSRGHCGTERASLMCHCT 1234
T FGP ++ GN+HTS K QTL+P HCT SR HC E+AS MCHCT
Sbjct: 860 TGVFGPYNRLPGNRHTSSKPQTLSPCHCTLSRAHCVKEKAS*MCHCT 1000
Score = 88.6 bits (187), Expect(3) = 2e-029
Identities = 48/80 (60%), Positives = 51/80 (63%)
Frame = -1 / -2
Query: 1234 CTMAHQRSSLCPAVSSAPSAMVRVQSLELHRGVLVAKVLALRSKSGSWGLE*SSKCSILA 1055
CTMAH RS L A+ SA SAM R QSL L V VA+ +RSK SW E* SKCS LA
Sbjct: 1000 CTMAHLRSFLFHAMCSAQSAMARAQSLGLR*SVPVAREPVIRSKYASWDQE*FSKCSNLA 821
Query: 1054 TSARVLERQSATRIDAHSAR 995
SA L R SATRID SAR
Sbjct: 820 MSAGELVRPSATRIDVGSAR 761
Score = 59.7 bits (124), Expect(3) = 2e-029
Identities = 26/44 (59%), Positives = 30/44 (68%)
Frame = -1 / -2
Query: 787 LNLCVASSLL*LTWITGSC*SNQIRAKL*SLILSRRSTTKACPC 656
L CVAS++ L W GSC S+ I KL SL SRRSTT+ACPC
Sbjct: 553 LKPCVASNMFLLIWTAGSCSSSPILGKLLSLTRSRRSTTRACPC 422
Score = 51.0 bits (105), Expect(3) = 1e-059
Identities = 25/41 (60%), Positives = 31/41 (75%)
Frame = +3 / +3
Query: 990 ITLALWASILVADCLSRTLALVARMLHLLDHSRPQLPDLDL 1112
+ LAL SILVAD L+ + AL+AR+LHLL+HS QL LDL
Sbjct: 756 LALALPTSILVADGLTSSPALIARLLHLLNHSWSQLAYLDL 878
Score = 47.8 bits (98), Expect(3) = 8e-091
Identities = 19/24 (79%), Positives = 20/24 (83%)
Frame = -3 / -1
Query: 476 EAEMRRRQQQHQEAYDEDEDMPGG 405
E RR+QQQ QEAYDEDEDMPGG
Sbjct: 239 EEMRRRQQQQAQEAYDEDEDMPGG 168
Score = 36.8 bits (74), Expect(3) = 8e-091
Identities = 16/24 (66%), Positives = 16/24 (66%)
Frame = -1 / -2
Query: 571 PWRLCSRPSRCRSTPTWSWTSARR 500
P RL RPS CRST TWS S RR
Sbjct: 337 P*RLSCRPSPCRSTRTWS*MSVRR 266
Score = 34.5 bits (69), Expect(2) = e-139
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +1 / +1
Query: 364 QAAAYCWAHCTLCAPP 411
Q ++YCWAHCT PP
Sbjct: 124 QTSSYCWAHCTRWPPP 171
Score = 29.5 bits (58), Expect(3) = 3e-088
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +3 / +3
Query: 861 NKDDISSDSVRRFISFTRECDLLPI 935
++D + S+ +RRFI RE DLL +
Sbjct: 627 DEDYVPSNGIRRFIGLAREGDLLAV 701
Score = 26.7 bits (52), Expect(3) = 1e-059
Identities = 14/21 (66%), Positives = 16/21 (76%)
Frame = +3 / +3
Query: 1173 IALGAEDTAGQRELL*CAIVQ 1235
IAL AE A +R+LL CAIVQ
Sbjct: 939 IAL*AEHIA*KRKLLRCAIVQ 1001
Score = 21.7 bits (41), Expect(3) = 2e-029
Identities = 9/16 (56%), Positives = 10/16 (62%)
Frame = -1 / -2
Query: 940 CRMGRRSHSLVKLMKR 893
C RRS SL + MKR
Sbjct: 706 CSTARRSPSLARPMKR 659
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 18,884,057
Number of Sequences: 8143
Number of extensions: 295990
Number of successful extensions: 2575
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 617340728
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)