TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192481.2.2
(1053 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821538.1|CN821538 HRO4426_D11_G22ZS5 Lib AA069E1X Aven... 60 8e-038
gb|CN818485.1|CN818485 HRO4460_C01_E02ZS5 Lib AA070E1X Aven... 136 2e-037
gb|CN816389.1|CN816389 HRO4520_B08_D16ZS5 Lib AA071E1X Aven... 133 7e-032
gb|CN820431.1|CN820431 HRO4419_C08_F15ZS5 Lib AA069E1X Aven... 124 2e-029
>gb|CN821538.1|CN821538 HRO4426_D11_G22ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4426_D11_G22, mRNA
sequence
Length = 681
Score = 60.2 bits (125), Expect(5) = 8e-038
Identities = 25/55 (45%), Positives = 37/55 (67%)
Frame = -2 / +1
Query: 350 VPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN*IKPALHPP 186
+ ++ KIF GV HG+ RY++ AVK A ALA+M +WF+K+L * K ++ PP
Sbjct: 409 IVHFVKIFPGVSHGWTVRYDS*YSAAVKCAHEALAHMNSWFDKNLK*TK*SISPP 573
Score = 50.1 bits (103), Expect(5) = 8e-038
Identities = 21/36 (58%), Positives = 26/36 (72%)
Frame = -2 / +1
Query: 473 VTADDMKEVKWPIEILGAQNDTTTPPKEVYRFVHVL 366
VT DD+KEVK PI ILGA+ D +PP+ V + HVL
Sbjct: 283 VTVDDIKEVKSPIAILGAETDVMSPPELVKQIQHVL 390
Score = 43.7 bits (89), Expect(5) = 8e-038
Identities = 16/32 (50%), Positives = 23/32 (71%)
Frame = -2 / +3
Query: 665 KNFMEWLEAHSPVKAAEDAKPLFAALKKEGKS 570
K F EW++ H+P K E AKP+ +ALK++G S
Sbjct: 87 KPFAEWIKEHAPEKGFEKAKPVISALKEQGVS 182
Score = 36.3 bits (73), Expect(5) = 8e-038
Identities = 13/18 (72%), Positives = 15/18 (83%)
Frame = -2 / +2
Query: 734 KVAKAGYFVVVPDFLKGD 681
+VA +GYFVVVPDF GD
Sbjct: 8 RVASSGYFVVVPDFFNGD 61
Score = 35.0 bits (70), Expect(5) = 8e-038
Identities = 10/32 (31%), Positives = 20/32 (62%)
Frame = -2 / +3
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPY 477
SV YCWG K+ ++ K+++++A + H +
Sbjct: 183 SVGAACYCWGAKVIADLAKSNEIQAAVMLHHF 278
>gb|CN818485.1|CN818485 HRO4460_C01_E02ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4460_C01_E02, mRNA sequence
Length = 674
Score = 136 bits (292), Expect(2) = 2e-037
Identities = 53/122 (43%), Positives = 76/122 (62%)
Frame = -2 / +1
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPK 393
++ GYCWG K V++ K ++ A + HP + DD+KEVK PI ILGA+ D T+PP+
Sbjct: 139 TIGAAGYCWGAKGVVDLAKAREIHAAVMLHPSRIPVDDIKEVKCPIAILGAEIDQTSPPE 318
Query: 392 EVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN 213
+ F +L + ++ KIF GV HG+ RYN D A+K+AE AL M +WF+K+L
Sbjct: 319 LIKEFEQILSSNSGINHFVKIFPGVSHGWTMRYNNDDAAAIKSAEEALTDMTDWFDKYLK 498
Query: 212 *I 207
*I
Sbjct: 499 *I 504
Score = 95.9 bits (203), Expect = 1e-020
Identities = 55/117 (47%), Positives = 61/117 (52%)
Frame = +1 / -1
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
I R L NQ M RA SA+L A S +LYL +P TP KIL * L C+N
Sbjct: 503 IHFRYLSNQSVMSVRASSALLIAAASSLLYLIVHP*ETPGKILTK*LIPELEERICSNSL 324
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ*P 558
S GG V S AP I+IGHFTS +SS GC A S F ST F PQQ*P
Sbjct: 323 ISSGGEVWSISAPSIAIGHFTSLISSTGIREGCNITAACISRAFAKSTTPFAPQQ*P 153
Score = 35.0 bits (70), Expect(2) = 2e-037
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -2 / +1
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEG 576
W++ H K E+AKP+ A+LK++G
Sbjct: 58 WIQKHPQAKLFEEAKPIIASLKEQG 132
>gb|CN816389.1|CN816389 HRO4520_B08_D16ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_B08_D16, mRNA sequence
Length = 551
Score = 133 bits (284), Expect = 7e-032
Identities = 54/106 (50%), Positives = 73/106 (68%)
Frame = -2 / +2
Query: 527 EVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPKEVYRFVHVLRERHEV 348
E+ K ++++A +SHP VT DD+KEVK PI +LGA+ D T+PP+ V +F VL E+
Sbjct: 14 ELAKANEIQAAVMSHPSFVTVDDIKEVKCPIAVLGAETDRTSPPELVKQFEQVLSSNSEI 193
Query: 347 PYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN* 210
Y+ KIF GV HG+ RY++ D AVK+AE AL M NWFNK+L *
Sbjct: 194 GYFVKIFPGVSHGWTVRYDSDDAAAVKSAEEALTDMTNWFNKNLK* 331
Score = 96.4 bits (204), Expect = 7e-021
Identities = 51/109 (46%), Positives = 63/109 (57%)
Frame = +1 / -3
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
+ LR LLNQL M +A SA+ TA S + YL +P TP KIL * S L TC+N
Sbjct: 333 VHLRFLLNQLVMSVKASSALFTAAASSLSYLTVHP*DTPGKILTK*PISELEDRTCSNCL 154
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTL 534
T+ GG V+S AP ++GH TS +SS VT GC+ A SL F S L
Sbjct: 153 TNSGGDVLSVSAPSTAMGHLTSLISSTVTNEGCDITAAWISLAFANSAL 7
>gb|CN820431.1|CN820431 HRO4419_C08_F15ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4419_C08_F15, mRNA
sequence
Length = 560
Score = 124 bits (266), Expect = 2e-029
Identities = 53/108 (49%), Positives = 71/108 (65%)
Frame = -2 / +1
Query: 533 SVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPKEVYRFVHVLRERH 354
S V K+++++A +SHP VT DD+KEVK PI ILGA+ D +PP+ V +F VL
Sbjct: 7 SCRVSKSNEIQAAVMSHPSFVTVDDIKEVKCPIAILGAETDVMSPPELVKQFKQVLSSNA 186
Query: 353 EVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN* 210
+ + KIF GV HG+ RY++ D AVK AE ALA M+ WFNK+L *
Sbjct: 187 GIGSFVKIFPGVSHGWTVRYDSNDAAAVKNAEEALADMIEWFNKNLK* 330
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,962,186
Number of Sequences: 8143
Number of extensions: 124911
Number of successful extensions: 966
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 370013670
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)