TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161285.2.2
(798 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815151.1|CN815151 HRO4506_D10_G20ZS5 Lib AA071E1X Aven... 183 2e-047
gb|CN818949.1|CN818949 HRO4474_G09_N18ZS5 Lib AA070E1X Aven... 170 2e-043
>gb|CN815151.1|CN815151 HRO4506_D10_G20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4506_D10_G20, mRNA sequence
Length = 599
Score = 183 bits (395), Expect = 2e-047
Identities = 79/103 (76%), Positives = 90/103 (87%)
Frame = +1 / +1
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLGASVNALGSTATVLTSGNVMDENSFSNP 360
ITWQD+ NSFLRVK+VNFGSD V+LT+S +GL AS+N LGS ATVLTS NVMDENSFSNP
Sbjct: 25 ITWQDSGNSFLRVKIVNFGSDQVSLTISATGLQASINTLGSNATVLTSSNVMDENSFSNP 204
Query: 361 NKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLALAEPKLVAEI* 489
NKV PV SQL NAAEQMQVTL HSF++FDLALA+ +LVAE+*
Sbjct: 205 NKVVPVTSQLRNAAEQMQVTLPAHSFSSFDLALAQSQLVAEM* 333
Score = 174 bits (375), Expect = 1e-044
Identities = 82/110 (74%), Positives = 85/110 (77%)
Frame = -2 / -1
Query: 488 HISATSLGSASARSKAVKE*GASVTCICSAALLSWLFTGATLFGLLKEFSSMTLPDVRTV 309
HISATS ASARS KE* SVTCICSAAL SWL TG TLFGLLKEFSS+TL DVRTV
Sbjct: 332 HISATSCD*ASARSNEEKE*AGSVTCICSAALRSWLVTGTTLFGLLKEFSSITLLDVRTV 153
Query: 308 AVDPSALTLAPSPLVETVRLTVSDPKFTTFTLRKLFSASCHVIADAASEL 159
A DP L LA SP+ E VRLT S+PKFT FTLRKLF SC VIADAA EL
Sbjct: 152 AFDPRVLMLA*SPVAEIVRLTWSEPKFTIFTLRKLFPESCQVIADAAREL 3
Score = 126 bits (270), Expect = 4e-030
Identities = 63/111 (56%), Positives = 75/111 (67%)
Frame = -1 / -3
Query: 489 SYLRHKLGLSQRQIEGGEGVRSQRDLHLLRGVAQLAFHRRNLVRVTEGVLVHDVARREDG 310
S+LR KL LSQR++E GEGV + DLHLLR VAQLA HR NLV VTEG+ +H +A E+
Sbjct: 333 SHLRDKL*LSQRKVERGEGVSRECDLHLLRSVAQLACHRHNLVWVTEGIFIHHIAGCENS 154
Query: 309 GG*SQRVDAGPESARGDREVNGVRPEVHDLHSQEAILCILPRDRRRGQRTR 157
VDA + R D E + VR +VH+LHSQEAI ILP D R QRTR
Sbjct: 153 SVRP*SVDASLKPGRRDCEAHLVRTKVHNLHSQEAIPRILPGDSRCCQRTR 1
Score = 114 bits (244), Expect = 2e-026
Identities = 55/109 (50%), Positives = 68/109 (62%)
Frame = +3 / +3
Query: 162 FAGRVCDHVAGCRE*LPESEGRELRVGHR*PHGLHERTRGQRQRAGINRHRPHVWQRHGR 341
F+G +C H+AG E*LPESE EL G PH L +R G Q + + R+ H+ Q G
Sbjct: 6 FSGSICYHLAGFWE*LPESEDCELWFGPGEPHNLCDRASG*HQHSRVERYCSHIQQCDG* 185
Query: 342 ELLQ*PEQGCAGEKPAEQRRGADAGHAGSSLLHRLRSGAG*AQACGGDM 488
+ LQ*P+QGCA +KPA QR GADAGH SLL +R AG AC GD+
Sbjct: 186 KFLQ*PKQGCACDKPAAQRCGADAGHTPCSLLLLVRPCAGSVTACRGDV 332
>gb|CN818949.1|CN818949 HRO4474_G09_N18ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4474_G09_N18, mRNA sequence
Length = 495
Score = 170 bits (366), Expect = 2e-043
Identities = 74/96 (77%), Positives = 85/96 (88%)
Frame = +1 / +3
Query: 202 NSFLRVKVVNFGSDTVNLTVSTSGLGASVNALGSTATVLTSGNVMDENSFSNPNKVAPVK 381
NSFLRVK+VNFGSD V+LT++ +GL AS+NALGS ATVLTS NVMDENSFSNPNKV PV
Sbjct: 12 NSFLRVKIVNFGSDQVSLTITATGLQASINALGSNATVLTSSNVMDENSFSNPNKVVPVT 191
Query: 382 SQLSNAAEQMQVTLAPHSFTAFDLALAEPKLVAEI* 489
SQL NAAEQMQVTL HSF++FDLALA+ +LVAE+*
Sbjct: 192 SQLRNAAEQMQVTLPAHSFSSFDLALAQSQLVAEM* 299
Score = 153 bits (328), Expect = 4e-038
Identities = 72/96 (75%), Positives = 76/96 (79%)
Frame = -2 / -3
Query: 488 HISATSLGSASARSKAVKE*GASVTCICSAALLSWLFTGATLFGLLKEFSSMTLPDVRTV 309
HISATS ASARS KE* SVTCICSAAL SWL TG TLFGLLKEFSS+TL DVRTV
Sbjct: 298 HISATSCD*ASARSNEEKE*AGSVTCICSAALRSWLVTGTTLFGLLKEFSSITLLDVRTV 119
Query: 308 AVDPSALTLAPSPLVETVRLTVSDPKFTTFTLRKLF 201
A +PSAL LA SP+ VRLT S+PKFT FTLRKLF
Sbjct: 118 AFEPSALMLA*SPVAVIVRLTWSEPKFTIFTLRKLF 11
Score = 127 bits (271), Expect = 3e-030
Identities = 57/96 (59%), Positives = 71/96 (73%)
Frame = -1 / -2
Query: 489 SYLRHKLGLSQRQIEGGEGVRSQRDLHLLRGVAQLAFHRRNLVRVTEGVLVHDVARREDG 310
S+LR KL LSQR++E GEGV + DLHLLR VAQLA HR NLVRVTEG+ +H +A R++
Sbjct: 299 SHLRDKL*LSQRKVERGEGVSRECDLHLLRSVAQLACHRHNLVRVTEGIFIHHIAGRQNS 120
Query: 309 GG*SQRVDAGPESARGDREVNGVRPEVHDLHSQEAI 202
+QRVDA + R D E + VR +VH+LHSQEAI
Sbjct: 119 SVRTQRVDASLKPGRSDCEAHLVRTKVHNLHSQEAI 12
Score = 104 bits (221), Expect = 2e-023
Identities = 54/99 (54%), Positives = 60/99 (60%)
Frame = +3 / +2
Query: 192 GCRE*LPESEGRELRVGHR*PHGLHERTRGQRQRAGINRHRPHVWQRHGRELLQ*PEQGC 371
G RE*LPESE EL G PH +R G QRAG R+ V Q G + LQ*PEQGC
Sbjct: 2 GTRE*LPESEDCELWFGPGEPHNHCDRASG*HQRAGFERYCSDVQQCDG*KFLQ*PEQGC 181
Query: 372 AGEKPAEQRRGADAGHAGSSLLHRLRSGAG*AQACGGDM 488
A +KPA QR GADAGH SLL +R AG AC GD+
Sbjct: 182 ACDKPAAQRCGADAGHTPCSLLLLVRPCAGSVTACRGDV 298
Score = 95.5 bits (202), Expect = 1e-020
Identities = 58/108 (53%), Positives = 64/108 (59%)
Frame = +2 / +1
Query: 191 RMQRIAS*E*RS*TSGRTPLTSRSPRADSGPASTRWDQPPPSSRLATSWTRTPSVTRTRL 370
R + IAS*E*R *T RT S+S R ASTRW + R A W + PSVTRTRL
Sbjct: 1 RHEGIAS*E*RL*TLVRTR*ASQSLRPGFRLASTRWVRTLLF*RPAM*WMKIPSVTRTRL 180
Query: 371 RR*KAS*ATPRSRCRSRWLLTPSPPSIWRWLSPSLWRRYDVCAAHYST 514
*+AS AT RSRCRS LLTPSP S RWLS SL RR + T
Sbjct: 181 CL*QASCATLRSRCRSHSLLTPSPRSTLRWLSHSLSRRCEAATQRSET 324
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,174,480
Number of Sequences: 8143
Number of extensions: 155085
Number of successful extensions: 1580
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 270249036
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)