TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161285.2.1
(780 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815151.1|CN815151 HRO4506_D10_G20ZS5 Lib AA071E1X Aven... 185 9e-048
gb|CN818949.1|CN818949 HRO4474_G09_N18ZS5 Lib AA070E1X Aven... 171 9e-044
>gb|CN815151.1|CN815151 HRO4506_D10_G20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4506_D10_G20, mRNA sequence
Length = 599
Score = 185 bits (398), Expect = 9e-048
Identities = 80/103 (77%), Positives = 90/103 (87%)
Frame = -3 / +1
Query: 484 ITWQDAENSFLRVKVVNFGSDPVSLTVSTSGLGASVNALGSTATVLTSGNVMDENSFSNP 305
ITWQD+ NSFLRVK+VNFGSD VSLT+S +GL AS+N LGS ATVLTS NVMDENSFSNP
Sbjct: 25 ITWQDSGNSFLRVKIVNFGSDQVSLTISATGLQASINTLGSNATVLTSSNVMDENSFSNP 204
Query: 304 NKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLALAEPKLVAEI* 176
NKV PV SQL NAAEQMQVTL HSF++FDLALA+ +LVAE+*
Sbjct: 205 NKVVPVTSQLRNAAEQMQVTLPAHSFSSFDLALAQSQLVAEM* 333
Score = 175 bits (376), Expect = 1e-044
Identities = 82/110 (74%), Positives = 85/110 (77%)
Frame = +3 / -1
Query: 177 HISATSLGSASARSKAVKE*GASVTCICSAALLSWLFTGATLFGLLKEFSSMTLPDVRTV 356
HISATS ASARS KE* SVTCICSAAL SWL TG TLFGLLKEFSS+TL DVRTV
Sbjct: 332 HISATSCD*ASARSNEEKE*AGSVTCICSAALRSWLVTGTTLFGLLKEFSSITLLDVRTV 153
Query: 357 AVDPSALTLAPSPLVETVRLTGSDPKFTTFTLRKLFSASCHVIADAASEL 506
A DP L LA SP+ E VRLT S+PKFT FTLRKLF SC VIADAA EL
Sbjct: 152 AFDPRVLMLA*SPVAEIVRLTWSEPKFTIFTLRKLFPESCQVIADAAREL 3
Score = 135 bits (289), Expect = 1e-032
Identities = 67/117 (57%), Positives = 80/117 (68%)
Frame = +2 / -3
Query: 158 SLTAHTSYLRHKLGLSQRQIEGGEGVRSQRDLHLLRGVAQLAFHRRNLVRVTEGVLVHDV 337
SL+ S+LR KL LSQR++E GEGV + DLHLLR VAQLA HR NLV VTEG+ +H +
Sbjct: 351 SLSVAASHLRDKL*LSQRKVERGEGVSRECDLHLLRSVAQLACHRHNLVWVTEGIFIHHI 172
Query: 338 ARREDGGG*SQRVDAGPEPARGDREANGVRPEVHDLHSQEAILCILPRDRRRGQRTR 508
A E+ VDA +P R D EA+ VR +VH+LHSQEAI ILP D R QRTR
Sbjct: 171 AGCENSSVRP*SVDASLKPGRRDCEAHLVRTKVHNLHSQEAIPRILPGDSRCCQRTR 1
Score = 120 bits (257), Expect = 3e-028
Identities = 57/109 (52%), Positives = 70/109 (64%)
Frame = -2 / +3
Query: 503 FAGRVCDHVAGCRE*LPESEGRELRVGPR*PHGLHERARGQRQRAGINRHRPHVWQRHGR 324
F+G +C H+AG E*LPESE EL GP PH L +RA G Q + + R+ H+ Q G
Sbjct: 6 FSGSICYHLAGFWE*LPESEDCELWFGPGEPHNLCDRASG*HQHSRVERYCSHIQQCDG* 185
Query: 323 ELLQ*PEQGCAGEKPAEQRRGADAGHAGSSLLHRLRSGAG*AQACGGDM 177
+ LQ*P+QGCA +KPA QR GADAGH SLL +R AG AC GD+
Sbjct: 186 KFLQ*PKQGCACDKPAAQRCGADAGHTPCSLLLLVRPCAGSVTACRGDV 332
Score = 100 bits (213), Expect = 3e-022
Identities = 62/116 (53%), Positives = 69/116 (59%)
Frame = -1 / +2
Query: 504 VRWPRLRSRGRMQRIAS*E*RS*TSGRTPLASRSPRAGSGPASTRWDQPPPSSRLATSWT 325
V W L S GR+ IAS*E*R *T RT AS+S R G AST + S A W
Sbjct: 5 VLWQHLLSPGRILGIAS*E*RL*TLVRTR*ASQSLRPGFRLASTL*GRTLLFSHPAM*WM 184
Query: 324 RTPSVTRTRLRR*KAS*ATPRSRCRSRWLLTPSPPSIWRWLSPSLWRRYDVCAVSD 157
+ PSVT+TRL *+AS AT RSRCRS LLTPSP S RWLS SL RR + D
Sbjct: 185 KIPSVTQTRLCL*QASCATLRSRCRSHSLLTPSPRSTLRWLSHSLSRRCEAATDRD 352
>gb|CN818949.1|CN818949 HRO4474_G09_N18ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4474_G09_N18, mRNA sequence
Length = 495
Score = 171 bits (369), Expect = 9e-044
Identities = 75/96 (78%), Positives = 85/96 (88%)
Frame = -3 / +3
Query: 463 NSFLRVKVVNFGSDPVSLTVSTSGLGASVNALGSTATVLTSGNVMDENSFSNPNKVAPVK 284
NSFLRVK+VNFGSD VSLT++ +GL AS+NALGS ATVLTS NVMDENSFSNPNKV PV
Sbjct: 12 NSFLRVKIVNFGSDQVSLTITATGLQASINALGSNATVLTSSNVMDENSFSNPNKVVPVT 191
Query: 283 SQLSNAAEQMQVTLAPHSFTAFDLALAEPKLVAEI* 176
SQL NAAEQMQVTL HSF++FDLALA+ +LVAE+*
Sbjct: 192 SQLRNAAEQMQVTLPAHSFSSFDLALAQSQLVAEM* 299
Score = 153 bits (329), Expect = 3e-038
Identities = 72/96 (75%), Positives = 76/96 (79%)
Frame = +3 / -3
Query: 177 HISATSLGSASARSKAVKE*GASVTCICSAALLSWLFTGATLFGLLKEFSSMTLPDVRTV 356
HISATS ASARS KE* SVTCICSAAL SWL TG TLFGLLKEFSS+TL DVRTV
Sbjct: 298 HISATSCD*ASARSNEEKE*AGSVTCICSAALRSWLVTGTTLFGLLKEFSSITLLDVRTV 119
Query: 357 AVDPSALTLAPSPLVETVRLTGSDPKFTTFTLRKLF 464
A +PSAL LA SP+ VRLT S+PKFT FTLRKLF
Sbjct: 118 AFEPSALMLA*SPVAVIVRLTWSEPKFTIFTLRKLF 11
Score = 133 bits (284), Expect = 5e-032
Identities = 60/101 (59%), Positives = 74/101 (73%)
Frame = +2 / -2
Query: 161 LTAHTSYLRHKLGLSQRQIEGGEGVRSQRDLHLLRGVAQLAFHRRNLVRVTEGVLVHDVA 340
L S+LR KL LSQR++E GEGV + DLHLLR VAQLA HR NLVRVTEG+ +H +A
Sbjct: 314 LCVAASHLRDKL*LSQRKVERGEGVSRECDLHLLRSVAQLACHRHNLVRVTEGIFIHHIA 135
Query: 341 RREDGGG*SQRVDAGPEPARGDREANGVRPEVHDLHSQEAI 463
R++ +QRVDA +P R D EA+ VR +VH+LHSQEAI
Sbjct: 134 GRQNSSVRTQRVDASLKPGRSDCEAHLVRTKVHNLHSQEAI 12
Score = 110 bits (234), Expect = 4e-025
Identities = 56/99 (56%), Positives = 62/99 (62%)
Frame = -2 / +2
Query: 473 GCRE*LPESEGRELRVGPR*PHGLHERARGQRQRAGINRHRPHVWQRHGRELLQ*PEQGC 294
G RE*LPESE EL GP PH +RA G QRAG R+ V Q G + LQ*PEQGC
Sbjct: 2 GTRE*LPESEDCELWFGPGEPHNHCDRASG*HQRAGFERYCSDVQQCDG*KFLQ*PEQGC 181
Query: 293 AGEKPAEQRRGADAGHAGSSLLHRLRSGAG*AQACGGDM 177
A +KPA QR GADAGH SLL +R AG AC GD+
Sbjct: 182 ACDKPAAQRCGADAGHTPCSLLLLVRPCAGSVTACRGDV 298
Score = 99.6 bits (211), Expect = 6e-022
Identities = 59/98 (60%), Positives = 64/98 (65%)
Frame = -1 / +1
Query: 474 RMQRIAS*E*RS*TSGRTPLASRSPRAGSGPASTRWDQPPPSSRLATSWTRTPSVTRTRL 295
R + IAS*E*R *T RT AS+S R G ASTRW + R A W + PSVTRTRL
Sbjct: 1 RHEGIAS*E*RL*TLVRTR*ASQSLRPGFRLASTRWVRTLLF*RPAM*WMKIPSVTRTRL 180
Query: 294 RR*KAS*ATPRSRCRSRWLLTPSPPSIWRWLSPSLWRR 181
*+AS AT RSRCRS LLTPSP S RWLS SL RR
Sbjct: 181 CL*QASCATLRSRCRSHSLLTPSPRSTLRWLSHSLSRR 294
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,121,731
Number of Sequences: 8143
Number of extensions: 134598
Number of successful extensions: 2236
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 262945008
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)