TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161272.2.28
(3878 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Aven... 197 1e-050
gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Aven... 186 2e-047
gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Aven... 164 8e-041
gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Aven... 135 9e-038
>gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_G06_M12, mRNA sequence
Length = 521
Score = 197 bits (424), Expect = 1e-050
Identities = 72/107 (67%), Positives = 89/107 (83%)
Frame = -1 / +2
Query: 983 KWXXXXXXXXXXXXLNFIGVVAGISNAINNGYESWGPLFGKLFFAFWVIVHLYPFLKGLV 804
+W +N +G+VAG+S+A+NNGY SWGPLFGKLFF+FWVIVHLYPFLKGL+
Sbjct: 11 QWTTLLIPPTTLIIINMVGIVAGVSDAVNNGYGSWGPLFGKLFFSFWVIVHLYPFLKGLM 190
Query: 803 GRQNRTPTIVIVWSILLASIFSLLWVRIDPFLAKDDGPLLEECGLDC 663
GRQNRTPTIV++WS+LLASIFSL+WVRIDPF+AK GP+L+ CG+ C
Sbjct: 191 GRQNRTPTIVVLWSVLLASIFSLVWVRIDPFIAKSKGPILKPCGVQC 331
Score = 133 bits (285), Expect = 2e-031
Identities = 56/86 (65%), Positives = 61/86 (70%)
Frame = +1 / -3
Query: 673 PHSSNRGPSSFARKGSIRTQSSEKIEARRMDQTMTIVGVLFCLPTRPLRNG*RWTITQNA 852
PH GP A KGSI TQ+ EK+EA R DQ+ T+VGV FCLP PLRNG*RWT+TQ
Sbjct: 321 PHGLRMGPLDLAMKGSILTQTREKMEASRTDQSTTMVGVRFCLPMSPLRNG*RWTMTQKE 142
Query: 853 KKSFPNRGPHDSYPLLIALEMPATTP 930
KKSFP RGPHD PLL A E PAT P
Sbjct: 141 KKSFPKRGPHDP*PLLTASETPATMP 64
Score = 103 bits (219), Expect = 2e-022
Identities = 42/95 (44%), Positives = 68/95 (71%)
Frame = +3 / -2
Query: 654 LLVAIQTTLLQQGTIILRKERVDPDPKQREDRSQEDGPDNDNRWRPVLPPNQTLEERVKM 833
L + +T L+ G++ L E ++PDP + ED ++D P++D+ R VLPP++ LEE V++
Sbjct: 340 LCSTLYSTWLKDGSLGLGDEGINPDPDEGEDGGEQDRPEHDDGGRAVLPPHEPLEEWVEV 161
Query: 834 DDHPKCKEELPEQGAP*FISVVDRIGNASYHSNEV 938
DD P+ KEELPE+GAP* +++VD +GN S +++ V
Sbjct: 160 DDDPEGKEELPEEGAP*PVAIVDCVGNTSNNADHV 56
>gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C09_F17, mRNA
sequence
Length = 676
Score = 186 bits (401), Expect = 2e-047
Identities = 72/92 (78%), Positives = 82/92 (89%)
Frame = -1 / +1
Query: 938 NFIGVVAGISNAINNGYESWGPLFGKLFFAFWVIVHLYPFLKGLVGRQNRTPTIVIVWSI 759
N +GVVAG S AIN+GY+SWGPLFGKLFFAFWVIVHLYPFLKGL+GR NRTPTIVIVW+I
Sbjct: 49 NMVGVVAGTSYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAI 228
Query: 758 LLASIFSLLWVRIDPFLAKDDGPLLEECGLDC 663
LLASIFSLLWVRIDPF + GP ++ CG++C
Sbjct: 229 LLASIFSLLWVRIDPFTTRVAGPNIQTCGINC 324
Score = 127 bits (272), Expect = 1e-029
Identities = 57/80 (71%), Positives = 63/80 (78%)
Frame = +1 / -3
Query: 691 GPSSFARKGSIRTQSSEKIEARRMDQTMTIVGVLFCLPTRPLRNG*RWTITQNAKKSFPN 870
GP++ GSIRT S EKIEARR+ QTMTIVGV F LP RPLRNG + TITQ AKKSFPN
Sbjct: 296 GPATRVVNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TITQKAKKSFPN 117
Query: 871 RGPHDSYPLLIALEMPATTP 930
GPHD PLL+A E+PATTP
Sbjct: 116 SGPHDW*PLLMA*EVPATTP 57
>gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_H12_O23, mRNA
sequence
Length = 620
Score = 164 bits (353), Expect = 8e-041
Identities = 62/78 (79%), Positives = 71/78 (91%)
Frame = -1 / +2
Query: 896 NGYESWGPLFGKLFFAFWVIVHLYPFLKGLVGRQNRTPTIVIVWSILLASIFSLLWVRID 717
+GY+SWGPLFGKLFFAFWVIVHLYPFLKGL+GR NRTPTIVIVW+ILLASIFSLLWVRID
Sbjct: 2 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILLASIFSLLWVRID 181
Query: 716 PFLAKDDGPLLEECGLDC 663
PF + GP ++ CG++C
Sbjct: 182 PFTTRVAGPNIQTCGINC 235
Score = 106 bits (227), Expect = 2e-023
Identities = 48/69 (69%), Positives = 52/69 (75%)
Frame = +1 / -3
Query: 691 GPSSFARKGSIRTQSSEKIEARRMDQTMTIVGVLFCLPTRPLRNG*RWTITQNAKKSFPN 870
GP++ GSIRT S EKIEARR+ QTMTIVGV F LP RPLRNG + TITQ AKKSFP
Sbjct: 207 GPATRVVNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TITQKAKKSFPK 28
Query: 871 RGPHDSYPL 897
GPHD PL
Sbjct: 27 SGPHDW*PL 1
>gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_A01_A02, mRNA sequence
Length = 474
Score = 135 bits (290), Expect(3) = 9e-038
Identities = 54/60 (90%), Positives = 57/60 (95%)
Frame = -1 / +3
Query: 842 VIVHLYPFLKGLVGRQNRTPTIVIVWSILLASIFSLLWVRIDPFLAKDDGPLLEECGLDC 663
VIVHLYPFLKGL+GRQNRTPTIVIVWSILLASI SLLWVR++PFLAK DGP LEECGLDC
Sbjct: 12 VIVHLYPFLKGLLGRQNRTPTIVIVWSILLASIISLLWVRVNPFLAKTDGPTLEECGLDC 191
Score = 115 bits (245), Expect(3) = 4e-033
Identities = 48/60 (80%), Positives = 52/60 (86%)
Frame = +1 / -3
Query: 664 QSRPHSSNRGPSSFARKGSIRTQSSEKIEARRMDQTMTIVGVLFCLPTRPLRNG*RWTIT 843
QS+PHSS GPS ARKG RTQ SE +EARR+DQTMTIVGVLFCLP+RPLRNG*RWTIT
Sbjct: 190 QSKPHSSRVGPSVLARKGLTRTQRSEMMEARRIDQTMTIVGVLFCLPSRPLRNG*RWTIT 11
Score = 84.9 bits (179), Expect(3) = 2e-021
Identities = 38/65 (58%), Positives = 46/65 (70%)
Frame = +3 / -2
Query: 648 ADLLVAIQTTLLQQGTIILRKERVDPDPKQREDRSQEDGPDNDNRWRPVLPPNQTLEERV 827
+DLL IQ TLLQ + L KE VD PK+R+D QED PD+D+R PVLP QTLEE V
Sbjct: 206 SDLLGTIQATLLQGRAVSLGKEGVDAHPKERDDGGQED*PDDDDRRGPVLPSKQTLEEWV 27
Query: 828 KMDDH 842
+MD+H
Sbjct: 26 EMDNH 12
Score = 35.9 bits (72), Expect(3) = 4e-033
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +2 / -2
Query: 422 NCTRLPQPPVCPILH 466
NCTRL PP CPILH
Sbjct: 425 NCTRLSWPPACPILH 381
Score = 31.3 bits (62), Expect(3) = 9e-038
Identities = 11/15 (73%), Positives = 12/15 (80%)
Frame = -2 / +3
Query: 463 QYWANRRLRQPCAVR 419
QYWA R RQPCAV+
Sbjct: 384 QYWAGGRPRQPCAVQ 428
Score = 29.5 bits (58), Expect(3) = 2e-021
Identities = 11/16 (68%), Positives = 12/16 (75%)
Frame = +3 / -3
Query: 423 TAQGCRSLRFAQYCTH 470
TAQGCR L AQYC +
Sbjct: 424 TAQGCRGLPPAQYCIY 377
Score = 25.8 bits (50), Expect(3) = 4e-033
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +2 / -2
Query: 596 KHLQKYFFDHT 628
KHLQKY F HT
Sbjct: 278 KHLQKYSFGHT 246
Score = 25.4 bits (49), Expect(3) = 9e-038
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = -3 / +1
Query: 627 VWSKKYFCRCF 595
V K+YFCRCF
Sbjct: 247 VCPKEYFCRCF 279
Score = 22.1 bits (42), Expect(3) = 2e-021
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +1 / -1
Query: 589 GHKTSAKILLRPHT 630
G +T AKILLR +T
Sbjct: 285 GSETPAKILLRAYT 244
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 45,080,706
Number of Sequences: 8143
Number of extensions: 690769
Number of successful extensions: 3172
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 47
effective length of database: 1,184,766
effective search space used: 1475033670
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)