TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161200.2.1
(845 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821108.1|CN821108 HRO4427_F02_L03ZS5 Lib AA069E1X Aven... 239 1e-065
gb|CN821143.1|CN821143 HRO4428_A02_B04ZS5 Lib AA069E1X Aven... 228 2e-062
gb|CN819429.1|CN819429 HRO4405_H09_O17ZS5 Lib AA069E1X Aven... 131 1e-040
gb|CN816903.1|CN816903 HRO4526_A11_A22ZS5 Lib AA071E1X Aven... 96 6e-029
>gb|CN821108.1|CN821108 HRO4427_F02_L03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_F02_L03, mRNA
sequence
Length = 714
Score = 239 bits (517), Expect(2) = 1e-065
Identities = 95/128 (74%), Positives = 104/128 (81%)
Frame = -2 / +2
Query: 832 PXXYXXPXXYTIXVLXKXXXXQLDYIXXKNPLKMSYXVGFXNKYPKRPHHRGASIPHNGV 653
P Y P Y VL K Q+DYI KNP KMSY VG+ KYPKR HHRGASIPHNGV
Sbjct: 209 PGWYCGPNFYKTDVLRKFAKSQIDYILGKNPQKMSYVVGYGKKYPKRVHHRGASIPHNGV 388
Query: 652 KYGCKGGYKWRDTKKANPNILVGAMVAGPDRRDGYKDVRTNYNYTEPTLAANAGLVAALI 473
YGCKGG+KWR++KKANPNILVGAMVAGPDR DG+KD+RTNYNYTEPTLAANAGLVAALI
Sbjct: 389 HYGCKGGFKWRESKKANPNILVGAMVAGPDRHDGFKDIRTNYNYTEPTLAANAGLVAALI 568
Query: 472 SISDIKTG 449
S++DI TG
Sbjct: 569 SLADIDTG 592
Score = 128 bits (274), Expect(2) = 8e-034
Identities = 58/125 (46%), Positives = 74/125 (59%)
Frame = +2 / -3
Query: 449 SGFDIRYRNQCCNQTSVCRKGRLCXXXXXXXXXXXXTPVRASHHCSNKDVRVCLLRVPPF 628
+ D+ RNQCCNQ S+C K RLC PVRAS+HC ++D+ VCL R+PPF
Sbjct: 592 ASIDVS*RNQCCNQASICCKSRLCVIIVCADIFEAIMPVRASNHCPDEDIWVCLFRLPPF 413
Query: 629 VTSFASILDAVMRYRGTSVVRALRVLVXETHXVAHLQWVLXXDVVKLXXXXLAXDXYGVX 808
TSFAS++D VMR SV+ L VL+ T+ VAHL WVL +V+ L LA D +
Sbjct: 412 ETSFASVVDTVMRD*CPSVMNTLGVLLSITNDVAHLLWVLA*NVIDL*LCKLAEDIGFIE 233
Query: 809 XRXXV 823
R V
Sbjct: 232 IRSTV 218
Score = 127 bits (272), Expect = 2e-030
Identities = 61/107 (57%), Positives = 70/107 (65%)
Frame = -1 / +1
Query: 791 PXQVXXXXA*LHPXXEPIEDELRCGFRKQVPEAPSPQRCLDTS*RRQVWMQRRLQMEGHE 612
P QV LH EP +DELR K+VP+A S QR ++ S*R + MQRR QMEG
Sbjct: 250 PPQVCKVTDRLHSRQEPTKDELRRWLWKEVPQACSSQRGINPS*RCPLRMQRRFQMEGV* 429
Query: 611 EGKP*HPCWSNGGWP*QA*RLQRCPHELQLHRAYPCGKRWSGCSTDF 471
+GKP +P NG WP*QA* LQR PH+L LHRAY C K W GCSTDF
Sbjct: 430 KGKPKYPRRGNGCWP*QA*WLQRYPHKL*LHRAYSCSKCWLGCSTDF 570
Score = 100 bits (214), Expect = 2e-022
Identities = 56/112 (50%), Positives = 63/112 (56%)
Frame = -3 / +3
Query: 792 SXASXXXXSLTTSXXRTH*R*ATXWVSXTSTRSALTTEVPRYLITASSMDAKEVTNGGTR 613
S AS T RTH R*AT V ST S TE + LIT S+ DAKEV+NGG+
Sbjct: 249 SSASLQSHRSITF*ARTHKR*ATSLVMERSTPSVFITEGHQSLITVSTTDAKEVSNGGSL 428
Query: 612 RRQTLTSLLEQWWLALTGVXXXXXXXXXXXTQSLPLRQTLVWLQH*FLYLIS 457
+RQT S QW LALTG+ TQSL L+Q L WLQH*FL L S
Sbjct: 429 KRQTQISSSGQWLLALTGMMASKISAQTIITQSLLLQQMLAWLQH*FL*LTS 584
Score = 92.7 bits (196), Expect(2) = 6e-028
Identities = 45/57 (78%), Positives = 48/57 (84%)
Frame = +3 / -1
Query: 450 PVLISDIEISAATRPAFAARVGSV*L*FVRTSL*PSRLSGPATIAPTRMLGFAFFVS 620
PV +S EISAAT+PAFAARVGSV*L*FVR SL PS LSGPATIAPTR+ GFAF S
Sbjct: 591 PVSMSAREISAATKPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRIFGFAFLDS 421
Score = 46.4 bits (95), Expect(2) = 6e-028
Identities = 26/50 (52%), Positives = 29/50 (58%)
Frame = +3 / -1
Query: 666 GIEAPLW*GRFGYLFXKPTX*LIFNGFXXXM*SSXXXXXLXRTXMV*XXG 815
GI+APL * R GY F PT *LIF GF M*S L RT ++* G
Sbjct: 375 GIDAPL**TRLGYFFP*PTT*LIFCGFLPRM*SICDFANLRRTSVL*KLG 226
Score = 30.4 bits (60), Expect(2) = 8e-034
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = +3 / -1
Query: 342 FRGLSLYGFHADXXXXXXXXXXXXAEKMVFLSM 440
FR + +GF AD AEKMVFLS+
Sbjct: 702 FRDVLFHGFQADGGGVEFGNIGGTAEKMVFLSI 604
Score = 25.8 bits (50), Expect(2) = 1e-065
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2 / +2
Query: 448 RFGIDKNTIFSAI 410
R IDKNTIFSA+
Sbjct: 596 RTSIDKNTIFSAV 634
>gb|CN821143.1|CN821143 HRO4428_A02_B04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4428_A02_B04, mRNA
sequence
Length = 636
Score = 228 bits (493), Expect(3) = 2e-062
Identities = 89/112 (79%), Positives = 98/112 (87%)
Frame = -2 / +2
Query: 784 KXXXXQLDYIXXKNPLKMSYXVGFXNKYPKRPHHRGASIPHNGVKYGCKGGYKWRDTKKA 605
K Q+DYI KNP KMSY VG+ KYPKR HHRGASIPHNGV YGCKGG+KWR++KKA
Sbjct: 14 KFAKSQIDYILGKNPQKMSYVVGYGKKYPKRVHHRGASIPHNGVHYGCKGGFKWRESKKA 193
Query: 604 NPNILVGAMVAGPDRRDGYKDVRTNYNYTEPTLAANAGLVAALISISDIKTG 449
NPNILVGAMVAGPDR DG+KD+RTNYNYTEPTLAANAGLVAALIS++DI TG
Sbjct: 194 NPNILVGAMVAGPDRHDGFKDIRTNYNYTEPTLAANAGLVAALISLADIDTG 349
Score = 130 bits (279), Expect = 3e-031
Identities = 61/102 (59%), Positives = 71/102 (69%)
Frame = -1 / +1
Query: 761 LHPXXEPIEDELRCGFRKQVPEAPSPQRCLDTS*RRQVWMQRRLQMEGHEEGKP*HPCWS 582
LH EP +DEL C K+VP+A S QR ++ S*R + MQRR QMEG +GKP +P S
Sbjct: 37 LHSRQEPTKDELCCWLWKEVPKACSSQRGVNPS*RCPLRMQRRFQMEGV*KGKPKYPRRS 216
Query: 581 NGGWP*QA*RLQRCPHELQLHRAYPCGKRWSGCSTDFYI*YQ 456
NG WP*QA* LQR PH+L LHRAY C K W GCSTDF *Y+
Sbjct: 217 NGCWP*QA*WLQRYPHKL*LHRAYSCSKCWLGCSTDFSS*YR 342
Score = 127 bits (271), Expect(3) = 2e-034
Identities = 54/107 (50%), Positives = 69/107 (64%)
Frame = +2 / -3
Query: 449 SGFDIRYRNQCCNQTSVCRKGRLCXXXXXXXXXXXXTPVRASHHCSNKDVRVCLLRVPPF 628
+ DI RNQCCNQ S+C K RLC PVRAS+HCS++D+ VCL R+PPF
Sbjct: 349 ASIDIS*RNQCCNQASICCKSRLCVIIVCADIFEAIMPVRASNHCSDEDIWVCLFRLPPF 170
Query: 629 VTSFASILDAVMRYRGTSVVRALRVLVXETHXVAHLQWVLXXDVVKL 769
TSFAS++D VMR SV+ L VL+ T+ +AHL WVL +V+ L
Sbjct: 169 ETSFASVVDTVMRD*RPSVMNTLWVLLSITNNIAHLLWVLA*NVIDL 29
Score = 102 bits (218), Expect = 7e-023
Identities = 53/97 (54%), Positives = 61/97 (62%)
Frame = -3 / +3
Query: 747 RTH*R*ATXWVSXTSTRSALTTEVPRYLITASSMDAKEVTNGGTRRRQTLTSLLEQWWLA 568
RTH R*A V ST+S TE + LIT S+ DAKEV+NGG+ +RQT S EQW LA
Sbjct: 51 RTHKR*AMLLVMERSTQSVFITEGRQSLITVSTTDAKEVSNGGSLKRQTQISSSEQWLLA 230
Query: 567 LTGVXXXXXXXXXXXTQSLPLRQTLVWLQH*FLYLIS 457
LTG+ TQSL L+Q L WLQH*FL LIS
Sbjct: 231 LTGMMASKISAQTIITQSLLLQQMLAWLQH*FL*LIS 341
Score = 94.1 bits (199), Expect(2) = 2e-027
Identities = 46/57 (80%), Positives = 48/57 (84%)
Frame = +3 / -1
Query: 450 PVLISDIEISAATRPAFAARVGSV*L*FVRTSL*PSRLSGPATIAPTRMLGFAFFVS 620
PV IS EISAAT+PAFAARVGSV*L*FVR SL PS LSGPATIAPTR+ GFAF S
Sbjct: 348 PVSISAREISAATKPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRIFGFAFLDS 178
Score = 43.2 bits (88), Expect(2) = 2e-027
Identities = 22/33 (66%), Positives = 23/33 (69%)
Frame = +3 / -1
Query: 666 GIEAPLW*GRFGYLFXKPTX*LIFNGFXXXM*S 764
GI+APL * RFGY F PT *LIF GF M*S
Sbjct: 132 GIDAPL**TRFGYFFP*PTT*LIFCGFLPRM*S 34
Score = 33.6 bits (67), Expect(3) = 2e-034
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +3 / -1
Query: 339 IFRGLSLYGFHADXXXXXXXXXXXXAEKMVFLS 437
+FR ++L+GF AD AEKMVFLS
Sbjct: 462 MFRDVTLHGFQADGGGGGVGNIGGTAEKMVFLS 364
Score = 25.4 bits (49), Expect(3) = 2e-062
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -2 / +2
Query: 448 RFGIDKNTIFSAI 410
R +DKNTIFSA+
Sbjct: 353 RTSVDKNTIFSAV 391
Score = 22.6 bits (43), Expect(3) = 2e-062
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = -3 / +3
Query: 231 YFCTIMTDQYK 199
++C MTDQYK
Sbjct: 549 FYCIGMTDQYK 581
Score = 21.7 bits (41), Expect(3) = 2e-034
Identities = 7/15 (46%), Positives = 10/15 (66%)
Frame = +1 / -3
Query: 154 FHAMHTTSGLCMTED 198
FH +HT S +T+D
Sbjct: 625 FHKLHTLSSPWLTDD 581
>gb|CN819429.1|CN819429 HRO4405_H09_O17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4405_H09_O17, mRNA
sequence
Length = 698
Score = 131 bits (281), Expect(4) = 1e-040
Identities = 51/61 (83%), Positives = 59/61 (96%)
Frame = -2 / +2
Query: 631 YKWRDTKKANPNILVGAMVAGPDRRDGYKDVRTNYNYTEPTLAANAGLVAALISISDIKT 452
++WR++KKANPNILVGAMVAGPDR DG+KD+RTNYNYTEPTLAANAGLVAALIS++DI T
Sbjct: 14 FQWRESKKANPNILVGAMVAGPDRHDGFKDIRTNYNYTEPTLAANAGLVAALISLADIDT 193
Query: 451 G 449
G
Sbjct: 194 G 196
Score = 92.7 bits (196), Expect(3) = 2e-030
Identities = 45/57 (78%), Positives = 48/57 (84%)
Frame = +3 / -3
Query: 450 PVLISDIEISAATRPAFAARVGSV*L*FVRTSL*PSRLSGPATIAPTRMLGFAFFVS 620
PV +S EISAAT+PAFAARVGSV*L*FVR SL PS LSGPATIAPTR+ GFAF S
Sbjct: 195 PVSMSAREISAATKPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRIFGFAFLDS 25
Score = 76.2 bits (160), Expect(2) = 7e-021
Identities = 28/59 (47%), Positives = 36/59 (61%)
Frame = +2 / -2
Query: 449 SGFDIRYRNQCCNQTSVCRKGRLCXXXXXXXXXXXXTPVRASHHCSNKDVRVCLLRVPP 625
+ D+ RNQCCNQ S+C K RLC PVRAS+HC ++D+ VCL R+PP
Sbjct: 196 ASIDVS*RNQCCNQASICCKSRLCVIIVCADIFEAIMPVRASNHCPDEDIWVCLFRLPP 20
Score = 42.8 bits (87), Expect(3) = 2e-030
Identities = 20/40 (50%), Positives = 25/40 (62%)
Frame = +1 / -2
Query: 79 KVQFLQKITSHMSRNANSNKTPKISFHAMHTTSGLCMTED 198
KV FLQK +RN N+NK K FH +HTTS +T+D
Sbjct: 547 KVPFLQKELHA*TRNMNNNKDLKN*FHKLHTTSSPWLTDD 428
Score = 41.4 bits (84), Expect(2) = 7e-021
Identities = 21/44 (47%), Positives = 27/44 (61%)
Frame = +2 / -3
Query: 68 NNL*RYNFYKK*LHT*AGMPTATRPQKLAFTRCTQLLVYV*QRI 199
N+L R +FYKK G+ TR K++FT CTQL V+ *Q I
Sbjct: 558 NDLQRCHFYKKNFTRELGI*ITTRTSKISFTNCTQLPVHG*QMI 427
Score = 40.9 bits (83), Expect(4) = 1e-040
Identities = 19/45 (42%), Positives = 28/45 (62%)
Frame = -2 / +1
Query: 199 NPLSYIDQKLCASRES*FLGSCCCWHSCSCVKLFFVEIVPLQIVI 65
N LS +D KLCA E+ F G CC +S ++ FVE+ PL +++
Sbjct: 427 NHLSTMDWKLCAICETNF*GPCCYSYSEFTREVLFVEMAPL*VIL 561
Score = 33.6 bits (67), Expect(3) = 2e-030
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = +3 / -3
Query: 339 IFRGLSLYGFHADXXXXXXXXXXXXAEKMVFLSM 440
+FR + L+GF AD AEKMVFLS+
Sbjct: 309 LFRDVPLHGFQADGGGGGVGNIGGTAEKMVFLSI 208
Score = 25.8 bits (50), Expect(4) = 1e-040
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2 / +2
Query: 448 RFGIDKNTIFSAI 410
R IDKNTIFSA+
Sbjct: 200 RTSIDKNTIFSAV 238
Score = 23.1 bits (44), Expect(4) = 1e-040
Identities = 8/25 (32%), Positives = 16/25 (64%)
Frame = -3 / +3
Query: 273 ARNRKADSISL*SFYFCTIMTDQYK 199
++ K + ++ + ++C MTDQYK
Sbjct: 354 SKGHKQKADNILTEFYCIGMTDQYK 428
>gb|CN816903.1|CN816903 HRO4526_A11_A22ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4526_A11_A22, mRNA sequence
Length = 559
Score = 95.9 bits (203), Expect(3) = 6e-029
Identities = 38/44 (86%), Positives = 42/44 (95%)
Frame = -2 / -1
Query: 580 MVAGPDRRDGYKDVRTNYNYTEPTLAANAGLVAALISISDIKTG 449
MVAGPDR DG+KD+RTNYNYTEPTLAANAGLVAALIS++DI TG
Sbjct: 559 MVAGPDRHDGFKDIRTNYNYTEPTLAANAGLVAALISLADIDTG 428
Score = 70.3 bits (147), Expect(3) = 4e-024
Identities = 35/43 (81%), Positives = 37/43 (86%)
Frame = +3 / +3
Query: 450 PVLISDIEISAATRPAFAARVGSV*L*FVRTSL*PSRLSGPAT 578
PV +S EISAAT+PAFAARVGSV*L*FVR SL PS LSGPAT
Sbjct: 429 PVSMSAREISAATKPAFAARVGSV*L*FVRISLKPSCLSGPAT 557
Score = 42.8 bits (87), Expect(3) = 4e-024
Identities = 20/40 (50%), Positives = 25/40 (62%)
Frame = +1 / +2
Query: 79 KVQFLQKITSHMSRNANSNKTPKISFHAMHTTSGLCMTED 198
KV FLQK +RN N+NK K FH +HTTS +T+D
Sbjct: 77 KVPFLQKELHT*TRNMNNNKDLKN*FHKLHTTSSPWLTDD 196
Score = 41.8 bits (85), Expect(3) = 6e-029
Identities = 19/45 (42%), Positives = 28/45 (62%)
Frame = -2 / -3
Query: 199 NPLSYIDQKLCASRES*FLGSCCCWHSCSCVKLFFVEIVPLQIVI 65
N LS +D KLCA E+ F G CC +S ++ FVE+ PL +++
Sbjct: 197 NHLSTMDWKLCAICETNF*GPCCYSYSEFTCEVLFVEMAPL*VIL 63
Score = 33.6 bits (67), Expect(3) = 4e-024
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +3 / +3
Query: 339 IFRGLSLYGFHADXXXXXXXXXXXXAEKMVFLS 437
+FR ++L+GF AD AEKMVFLS
Sbjct: 315 MFRDVTLHGFQADGGGGGVGNIGGTAEKMVFLS 413
Score = 25.4 bits (49), Expect(3) = 6e-029
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -2 / -1
Query: 448 RFGIDKNTIFSAI 410
R +DKNTIFSA+
Sbjct: 424 RTSVDKNTIFSAV 386
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,576,039
Number of Sequences: 8143
Number of extensions: 118826
Number of successful extensions: 972
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 289726444
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)