TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1805139.2.3
(438 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN820687.1|CN820687 HRO4416_D03_H06ZS5 Lib AA069E1X Aven... 90 7e-033
gb|CN814680.1|CN814680 HRO4501_D01_G01ZS5 Lib AA071E1X Aven... 90 1e-032
>gb|CN820687.1|CN820687 HRO4416_D03_H06ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4416_D03_H06, mRNA
sequence
Length = 516
Score = 90.0 bits (190), Expect(2) = 7e-033
Identities = 33/35 (94%), Positives = 34/35 (97%)
Frame = -3 / +2
Query: 106 QVTVNNTMYHKSCFKCCHGGCTISPSNYIAHEGKL 2
+VTVNNTMYHKSCFKC HGGCTISPSNYIAHEGKL
Sbjct: 83 RVTVNNTMYHKSCFKCSHGGCTISPSNYIAHEGKL 187
Score = 79.4 bits (167), Expect(2) = 4e-028
Identities = 31/34 (91%), Positives = 31/34 (91%)
Frame = +3 / -1
Query: 3 SFPSCAM*LEGLMVHPPWQHLKQLLWYIVLLTVT 104
S PSCAM* EGLMVHPPW HLKQLLWYIVLLTVT
Sbjct: 186 SLPSCAM*FEGLMVHPPWLHLKQLLWYIVLLTVT 85
Score = 64.8 bits (135), Expect(2) = 5e-025
Identities = 25/34 (73%), Positives = 27/34 (79%)
Frame = -2 / +1
Query: 104 GHC*QHYVPQELLQVLPWRVHHQPF*LHCARGEA 3
G+C HYVPQELLQV PWRVHHQPF LH R +A
Sbjct: 85 GYCQ*HYVPQELLQVQPWRVHHQPFKLHRTRRQA 186
Score = 64.3 bits (134), Expect(2) = 7e-033
Identities = 24/29 (82%), Positives = 26/29 (89%)
Frame = -2 / +1
Query: 269 ECY*SLKRLCWHQREMCWMQQDSLSN*EG 183
ECY*SL+ +CWHQREM WMQQDSL N*EG
Sbjct: 1 ECY*SLELICWHQREMFWMQQDSLPN*EG 87
Score = 62.9 bits (131), Expect(2) = 5e-025
Identities = 25/29 (86%), Positives = 26/29 (89%)
Frame = -3 / +2
Query: 268 NATKVSSAFAGTREKCVGCSKTVYPTERV 182
NA KVSS+FAGTREKC GCSKTVYP ERV
Sbjct: 2 NAIKVSSSFAGTREKCFGCSKTVYPIERV 88
Score = 58.8 bits (122), Expect(2) = 4e-028
Identities = 24/30 (80%), Positives = 27/30 (90%)
Frame = +3 / -1
Query: 180 LTLSVG*TVLLHPTHFSLVPAKALETLVAF 269
+TLS+G*TVLLHP HFSLVPA LETL+AF
Sbjct: 90 VTLSIG*TVLLHPKHFSLVPANELETLIAF 1
>gb|CN814680.1|CN814680 HRO4501_D01_G01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4501_D01_G01, mRNA sequence
Length = 565
Score = 90.0 bits (190), Expect(2) = 1e-032
Identities = 33/35 (94%), Positives = 34/35 (97%)
Frame = -3 / +3
Query: 106 QVTVNNTMYHKSCFKCCHGGCTISPSNYIAHEGKL 2
+VTVNNTMYHKSCFKC HGGCTISPSNYIAHEGKL
Sbjct: 186 RVTVNNTMYHKSCFKCSHGGCTISPSNYIAHEGKL 290
Score = 79.4 bits (167), Expect(2) = 5e-028
Identities = 31/34 (91%), Positives = 31/34 (91%)
Frame = +3 / -1
Query: 3 SFPSCAM*LEGLMVHPPWQHLKQLLWYIVLLTVT 104
S PSCAM* EGLMVHPPW HLKQLLWYIVLLTVT
Sbjct: 289 SLPSCAM*FEGLMVHPPWLHLKQLLWYIVLLTVT 188
Score = 64.3 bits (134), Expect(2) = 1e-024
Identities = 24/29 (82%), Positives = 25/29 (86%)
Frame = -2 / +2
Query: 89 HYVPQELLQVLPWRVHHQPF*LHCARGEA 3
HYVPQELLQV PWRVHHQPF LH RG+A
Sbjct: 203 HYVPQELLQVQPWRVHHQPFKLHRTRGQA 289
Score = 63.8 bits (133), Expect(2) = 1e-032
Identities = 25/30 (83%), Positives = 27/30 (90%)
Frame = -3 / +3
Query: 271 QNATKVSSAFAGTREKCVGCSKTVYPTERV 182
+NA KVSS+FAGTREKC GCSKTVYP ERV
Sbjct: 102 ENAIKVSSSFAGTREKCFGCSKTVYPIERV 191
Score = 62.5 bits (130), Expect(2) = 1e-024
Identities = 23/29 (79%), Positives = 26/29 (89%)
Frame = -2 / +2
Query: 269 ECY*SLKRLCWHQREMCWMQQDSLSN*EG 183
ECY*SL+ +CWHQREM WMQQDSL +*EG
Sbjct: 104 ECY*SLELICWHQREMLWMQQDSLPD*EG 190
Score = 58.8 bits (122), Expect(2) = 5e-028
Identities = 24/30 (80%), Positives = 27/30 (90%)
Frame = +3 / -1
Query: 180 LTLSVG*TVLLHPTHFSLVPAKALETLVAF 269
+TLS+G*TVLLHP HFSLVPA LETL+AF
Sbjct: 193 VTLSIG*TVLLHPKHFSLVPANELETLIAF 104
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,266,958
Number of Sequences: 8143
Number of extensions: 70459
Number of successful extensions: 2403
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 41
effective length of database: 1,233,624
effective search space used: 128296896
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)