TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1805139.2.1
(950 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN814680.1|CN814680 HRO4501_D01_G01ZS5 Lib AA071E1X Aven... 275 2e-078
gb|CN820687.1|CN820687 HRO4416_D03_H06ZS5 Lib AA069E1X Aven... 215 7e-060
>gb|CN814680.1|CN814680 HRO4501_D01_G01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4501_D01_G01, mRNA sequence
Length = 565
Score = 275 bits (594), Expect(2) = 2e-078
Identities = 108/130 (83%), Positives = 117/130 (90%)
Frame = -2 / +3
Query: 685 FDQLFKRTGSLDKSFEGTPKVVKPERNVGNENATKVSSAFAGTREKCVGCSKTVYPTERV 506
FDQL KRTGSLDK FEGTPKVVKPERN+ NENA KVSS+FAGTREKC GCSKTVYP ERV
Sbjct: 12 FDQLSKRTGSLDKIFEGTPKVVKPERNLENENAIKVSSSFAGTREKCFGCSKTVYPIERV 191
Query: 505 TVNNTMYHKSCFKCCHGGCTISPSNYIAHEGKLYCKHHHIQLIKEKGNFSQLENDHEKTS 326
TVNNTMYHKSCFKC HGGCTISPSNYIAHEGKL+C+HHH QLIKEKGNFSQLEND EK+S
Sbjct: 192 TVNNTMYHKSCFKCSHGGCTISPSNYIAHEGKLFCRHHHTQLIKEKGNFSQLENDQEKSS 371
Query: 325 QAGSLEEDEQ 296
Q+ ++E+
Sbjct: 372 QSAGSVDEEE 401
Score = 205 bits (442), Expect(3) = 1e-059
Identities = 88/117 (75%), Positives = 97/117 (82%)
Frame = -1 / +2
Query: 677 AVQEDRELGQELRRNSQGCQARKKRWE*ECY*SLKRLCWHQREMCWMQQDSLSN*EGHC* 498
A+QED ELGQ+LRRNSQGCQA KK E ECY*SL+ +CWHQREM WMQQDSL +*EG+
Sbjct: 20 ALQEDWELGQDLRRNSQGCQA*KKPRERECY*SLELICWHQREMLWMQQDSLPD*EGYRQ 199
Query: 497 *HYVPQELLQVLPWRVHHQPF*LHCARGEAVLQAPPHPADQGEGELQPA*E*PREDI 327
*HYVPQELLQV PWRVHHQPF LH RG+A+LQAP + A QGEGE QPA E*PRE I
Sbjct: 200 *HYVPQELLQVQPWRVHHQPFKLHRTRGQALLQAPSYSAHQGEGEFQPARE*PREVI 370
Score = 174 bits (374), Expect(2) = 7e-050
Identities = 72/102 (70%), Positives = 75/102 (73%)
Frame = +3 / -1
Query: 327 DVFSWSFSSWLKFPFSLISWMWWCLQYSFPSCAM*LEGLMVHPPWQHLKQLLWYIXXXXX 506
D FSWSFSSWLKFPFSL+S + WCLQ S PSCAM* EGLMVHPPW HLKQLLWYI
Sbjct: 370 DDFSWSFSSWLKFPFSLMS*V*WCLQKSLPSCAM*FEGLMVHPPWLHLKQLLWYIVLLTV 191
Query: 507 XXXXXXXXXXHPTHFSLVPAKALETLVAFSFPTFLSGLTTLG 632
HP HFSLVPA LETL+AFSF FLSGLTTLG
Sbjct: 190 TLSIG*TVLLHPKHFSLVPANELETLIAFSFSRFLSGLTTLG 65
Score = 143 bits (307), Expect(2) = 5e-037
Identities = 71/117 (60%), Positives = 74/117 (63%)
Frame = -3 / +1
Query: 678 SCSRGQGAWTRASKELPRLSSQKETLGMRMLLKSQAPLLAPERNVLDAARQSIQLRGSLL 499
S RG GAWTR+SKELPRLSS KET RMLLKS+A LLAPERN LDAARQS +LRG
Sbjct: 19 SSPRGLGAWTRSSKELPRLSSLKETSRTRMLLKSRAHLLAPERNALDAARQSTRLRGLPS 198
Query: 498 ITLCTTRAASSAAMEGAPSALLTTLXXXXXXXXXXXXXXXXXXXXXXXXXRMTTRRH 328
ITLCTTRAASSAAMEGAPSAL T RMT R H
Sbjct: 199 ITLCTTRAASSAAMEGAPSALQITSHTRASSSAGTIILSSSRRRGISASSRMTKRSH 369
Score = 138 bits (297), Expect(2) = 7e-035
Identities = 63/87 (72%), Positives = 74/87 (85%)
Frame = +2 / -3
Query: 425 NVVRRADGAPSMAALEAALVVHSVINSDPLSWIDCLAASNTFLSGASKGA*DFSSILIPN 604
+V+ RADGAPSMAALEAALVVHSVI+ +PL+ +DCLAAS FLSGASK A DF+SIL+
Sbjct: 272 DVI*RADGAPSMAALEAALVVHSVIDGNPLNRVDCLAASKAFLSGASK*ARDFNSILVLE 93
Query: 605 VSFWLDNLGSSFEALVQAPCPLEQLVE 685
VSF LDNLGSSFE LVQAP PL +L++
Sbjct: 92 VSFRLDNLGSSFEDLVQAPSPLGELIK 12
Score = 38.6 bits (78), Expect(2) = 7e-050
Identities = 17/35 (48%), Positives = 23/35 (65%)
Frame = +2 / -3
Query: 119 LKDNNVYMQIQDNNIIVL*THMTSIGKTEIEPRHR 223
LK N VYM+ +DNNIIVL* H+ ++ K + R
Sbjct: 554 LKYNKVYMRTKDNNIIVL*LHIMALEKLKSSASER 450
Score = 33.6 bits (67), Expect(2) = 2e-078
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -1 / +2
Query: 206 FQFYQWRSCVFREQ*YCCLVSACKRCYL* 120
F + C R Q Y CL+ ACK CY+*
Sbjct: 467 FSVFPMPLCAIRGQLYYCLLYACKPCYI* 553
Score = 31.3 bits (62), Expect(3) = 1e-059
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -2 / +3
Query: 205 FSFTNGGHVCSENNDIVVLYLHVNVVIFES 116
F F +V E+N I+V HVN+VIFE+
Sbjct: 468 FQFFQCHYVQLEDNYIIVFCTHVNLVIFEA 557
Score = 28.6 bits (56), Expect(2) = 5e-037
Identities = 14/22 (63%), Positives = 17/22 (77%)
Frame = -3 / +1
Query: 186 VMCVQRTMILLSCICM*TLLSL 121
+MC RT+ILLS + M*TLL L
Sbjct: 487 IMCN*RTIILLSFVRM*TLLYL 552
Score = 27.6 bits (54), Expect(3) = 1e-059
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -2 / +3
Query: 346 NDHEKTSQAGSLEEDEQEY*SLSTTTD 266
+ + + AGS++E+E EY*SL D
Sbjct: 354 DQEKSSQSAGSVDEEESEY*SLRIFRD 434
Score = 25.8 bits (50), Expect(2) = 7e-035
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +1 / -2
Query: 121 QR*QRLHADTRQQYHCSLNTHD 186
Q *Q LHA RQ Y+C L H+
Sbjct: 552 QI*QGLHAYKRQ*YNCPLIAHN 487
>gb|CN820687.1|CN820687 HRO4416_D03_H06ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4416_D03_H06, mRNA
sequence
Length = 516
Score = 215 bits (465), Expect(2) = 7e-060
Identities = 82/99 (82%), Positives = 90/99 (90%)
Frame = -2 / +2
Query: 592 NATKVSSAFAGTREKCVGCSKTVYPTERVTVNNTMYHKSCFKCCHGGCTISPSNYIAHEG 413
NA KVSS+FAGTREKC GCSKTVYP ERVTVNNTMYHKSCFKC HGGCTISPSNYIAHEG
Sbjct: 2 NAIKVSSSFAGTREKCFGCSKTVYPIERVTVNNTMYHKSCFKCSHGGCTISPSNYIAHEG 181
Query: 412 KLYCKHHHIQLIKEKGNFSQLENDHEKTSQAGSLEEDEQ 296
KL+C+HHH QLIKEKGNFSQLENDHEK+SQ+ ++E+
Sbjct: 182 KLFCRHHHTQLIKEKGNFSQLENDHEKSSQSAGSVDEEE 298
Score = 163 bits (350), Expect(3) = 2e-046
Identities = 67/89 (75%), Positives = 73/89 (82%)
Frame = -1 / +1
Query: 593 ECY*SLKRLCWHQREMCWMQQDSLSN*EGHC**HYVPQELLQVLPWRVHHQPF*LHCARG 414
ECY*SL+ +CWHQREM WMQQDSL N*EG+C *HYVPQELLQV PWRVHHQPF LH R
Sbjct: 1 ECY*SLELICWHQREMFWMQQDSLPN*EGYCQ*HYVPQELLQVQPWRVHHQPFKLHRTRR 180
Query: 413 EAVLQAPPHPADQGEGELQPA*E*PREDI 327
+A+LQAP + A QGEGE QPA E PRE I
Sbjct: 181 QALLQAPSYSAHQGEGEFQPARERPREVI 267
Score = 150 bits (322), Expect(3) = 1e-041
Identities = 61/89 (68%), Positives = 64/89 (71%)
Frame = +3 / -1
Query: 327 DVFSWSFSSWLKFPFSLISWMWWCLQYSFPSCAM*LEGLMVHPPWQHLKQLLWYIXXXXX 506
D FSWSFSSWLKFPFSL+S + WCLQ S PSCAM* EGLMVHPPW HLKQLLWYI
Sbjct: 267 DDFSWSFSSWLKFPFSLMS*V*WCLQKSLPSCAM*FEGLMVHPPWLHLKQLLWYIVLLTV 88
Query: 507 XXXXXXXXXXHPTHFSLVPAKALETLVAF 593
HP HFSLVPA LETL+AF
Sbjct: 87 TLSIG*TVLLHPKHFSLVPANELETLIAF 1
Score = 105 bits (224), Expect(2) = 7e-024
Identities = 53/88 (60%), Positives = 54/88 (61%)
Frame = -3 / +3
Query: 591 MLLKSQAPLLAPERNVLDAARQSIQLRGSLLITLCTTRAASSAAMEGAPSALLTTLXXXX 412
MLLKS+A LLAPERNVLDAARQS QLRG L ITLCTTRAASSAAMEGAPSAL T
Sbjct: 3 MLLKSRAHLLAPERNVLDAARQSTQLRGLLSITLCTTRAASSAAMEGAPSALQITSHTKA 182
Query: 411 XXXXXXXXXXXXXXXXXXXXXRMTTRRH 328
R TTR H
Sbjct: 183 SSSAGTIILSSSRRRGISASSRTTTRSH 266
Score = 103 bits (220), Expect(2) = 4e-024
Identities = 45/56 (80%), Positives = 52/56 (92%)
Frame = +2 / -3
Query: 425 NVVRRADGAPSMAALEAALVVHSVINSDPLSWIDCLAASNTFLSGASKGA*DFSSI 592
+V+ RADGAPSMAALEAALVVHSVI+S+PL+W+DCLAAS TFLSGASK A DF+SI
Sbjct: 169 DVI*RADGAPSMAALEAALVVHSVIDSNPLNWVDCLAASKTFLSGASK*ARDFNSI 2
Score = 30.4 bits (60), Expect(2) = 7e-060
Identities = 16/43 (37%), Positives = 18/43 (41%)
Frame = -1 / +3
Query: 227 YFCGVVRFQFYQWRSCVFREQ*YCCLVSACKRCYL*VRYSTWF 99
Y+ V F + RSC FR Y CLV YL WF
Sbjct: 369 YYLCVANFSVFPTRSCAFRG*LYYCLVRM*TLLYLSRDTFIWF 497
Score = 30.4 bits (60), Expect(3) = 1e-041
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +2 / -1
Query: 146 IQDNNIIVL*THMTSIGKTEIEPRHRNT 229
+QDNNII+L* HM ++ K NT
Sbjct: 450 VQDNNIIIL*MHMIALEKPRSWQHKDNT 367
Score = 28.6 bits (56), Expect(3) = 2e-046
Identities = 12/29 (41%), Positives = 20/29 (68%)
Frame = -3 / +2
Query: 186 VMCVQRTMILLSCICM*TLLSLSQILYLV 100
+MC+ R +ILLSC + ++ S+ +YLV
Sbjct: 410 IMCI*RIIILLSCTHVNLVVFESRYIYLV 496
Score = 28.1 bits (55), Expect(3) = 2e-046
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = -2 / +2
Query: 337 EKTSQAGSLEEDEQEY*SLSTTTDE 263
+ + AGS++E+E EY*SL D+
Sbjct: 260 KSSQSAGSVDEEESEY*SLRIFRDQ 334
Score = 24.9 bits (48), Expect(2) = 4e-024
Identities = 10/29 (34%), Positives = 16/29 (55%)
Frame = +1 / -3
Query: 100 NQVEYLTQR*QRLHADTRQQYHCSLNTHD 186
NQ+ + +++ TRQ Y+ LN HD
Sbjct: 496 NQINVSRLKYNKVYMRTRQ*YNYPLNAHD 410
Score = 22.6 bits (43), Expect(3) = 1e-041
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +2 / -1
Query: 215 RHRNT*CSRQMQEDSLF 265
+H++ Q+QEDSLF
Sbjct: 384 QHKDNTALEQLQEDSLF 334
Score = 22.1 bits (42), Expect(2) = 7e-024
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = -2 / +2
Query: 160 IVVLYLHVNVVIFES 116
I++ HVN+V+FES
Sbjct: 434 ILLSCTHVNLVVFES 478
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,920,659
Number of Sequences: 8143
Number of extensions: 158260
Number of successful extensions: 1069
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 332333274
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)