TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1804944.2.4
(667 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Aven... 197 1e-051
gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Aven... 187 1e-050
gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Aven... 164 6e-044
gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Aven... 137 2e-033
>gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_G06_M12, mRNA sequence
Length = 521
Score = 197 bits (425), Expect = 1e-051
Identities = 73/107 (68%), Positives = 88/107 (82%)
Frame = +3 / +2
Query: 9 KWXXXXXXXXXXXXXNFIGVVAGVSNAINNGYESWGPLFGKLFFAFWVIVHLYPFLKGLV 188
+W N +G+VAGVS+A+NNGY SWGPLFGKLFF+FWVIVHLYPFLKGL+
Sbjct: 11 QWTTLLIPPTTLIIINMVGIVAGVSDAVNNGYGSWGPLFGKLFFSFWVIVHLYPFLKGLM 190
Query: 189 GRQNRTPTIVIVWSILLASIFSLLWVRIDPFLAKDDGPLLEECGLDC 329
GRQNRTPTIV++WS+LLASIFSL+WVRIDPF+AK GP+L+ CG+ C
Sbjct: 191 GRQNRTPTIVVLWSVLLASIFSLVWVRIDPFIAKSKGPILKPCGVQC 331
Score = 141 bits (303), Expect = 9e-035
Identities = 61/92 (66%), Positives = 67/92 (72%)
Frame = -1 / -3
Query: 286 ARKGSIRTQRSEKIEASRMDQTMTIVGVLFCLPTKPLRKGYRWTITQNAKKSFPKRGPHD 107
A KGSI TQ EK+EASR DQ+ T+VGV FCLP PLR G RWT+TQ KKSFPKRGPHD
Sbjct: 288 AMKGSILTQTREKMEASRTDQSTTMVGVRFCLPMSPLRNG*RWTMTQKEKKSFPKRGPHD 109
Query: 106 SYPLLIALETPATTPMKFNRSKVVGGINKVVH 11
PLL A ETPAT P VVGGI++VVH
Sbjct: 108 P*PLLTASETPATMPTMLMMMSVVGGISRVVH 13
Score = 109 bits (233), Expect = 4e-025
Identities = 48/107 (44%), Positives = 72/107 (67%)
Frame = -3 / -2
Query: 326 IQTTLLKKWTIILCKERVNPNPKE*ED*SQQDGPDDDNCRCPVLPPNQTFEEGIQMDNHP 147
+ +T LK ++ L E +NP+P E ED +QD P+ D+ VLPP++ EE +++D+ P
Sbjct: 328 LYSTWLKDGSLGLGDEGINPDPDEGEDGGEQDRPEHDDGGRAVLPPHEPLEEWVEVDDDP 149
Query: 146 KCKEELPEEGAP*LVSIIDCIGNTSDHTNEVQ*EQSGRRYQ*GSPFK 6
+ KEELPEEGAP* V+I+DC+GNTS++ + V ++ Q*G P K
Sbjct: 148 EGKEELPEEGAP*PVAIVDCVGNTSNNADHVDDDECSWWDQ*GGPLK 8
>gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C09_F17, mRNA
sequence
Length = 676
Score = 187 bits (402), Expect(2) = 1e-050
Identities = 72/92 (78%), Positives = 82/92 (89%)
Frame = +3 / +1
Query: 54 NFIGVVAGVSNAINNGYESWGPLFGKLFFAFWVIVHLYPFLKGLVGRQNRTPTIVIVWSI 233
N +GVVAG S AIN+GY+SWGPLFGKLFFAFWVIVHLYPFLKGL+GR NRTPTIVIVW+I
Sbjct: 49 NMVGVVAGTSYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAI 228
Query: 234 LLASIFSLLWVRIDPFLAKDDGPLLEECGLDC 329
LLASIFSLLWVRIDPF + GP ++ CG++C
Sbjct: 229 LLASIFSLLWVRIDPFTTRVAGPNIQTCGINC 324
Score = 122 bits (262), Expect = 4e-029
Identities = 56/87 (64%), Positives = 61/87 (70%)
Frame = -1 / -3
Query: 277 GSIRTQRSEKIEASRMDQTMTIVGVLFCLPTKPLRKGYRWTITQNAKKSFPKRGPHDSYP 98
GSIRT EKIEA R+ QTMTIVGV F LP +PLR GY+ TITQ AKKSFP GPHD P
Sbjct: 272 GSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TITQKAKKSFPNSGPHDW*P 93
Query: 97 LLIALETPATTPMKFNRSKVVGGINKV 17
LL+A E PATTP VVGG+ V
Sbjct: 92 LLMA*EVPATTPTMLMIKIVVGGMRSV 12
Score = 29.5 bits (58), Expect(2) = 1e-050
Identities = 8/16 (50%), Positives = 12/16 (75%)
Frame = +3 / +3
Query: 477 PNIWDTLEGKWRLRQS 524
PN+WD L GKW + ++
Sbjct: 303 PNLWDQLLGKWEIAET 350
>gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_H12_O23, mRNA
sequence
Length = 620
Score = 164 bits (353), Expect(2) = 6e-044
Identities = 62/78 (79%), Positives = 71/78 (91%)
Frame = +3 / +2
Query: 96 NGYESWGPLFGKLFFAFWVIVHLYPFLKGLVGRQNRTPTIVIVWSILLASIFSLLWVRID 275
+GY+SWGPLFGKLFFAFWVIVHLYPFLKGL+GR NRTPTIVIVW+ILLASIFSLLWVRID
Sbjct: 2 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILLASIFSLLWVRID 181
Query: 276 PFLAKDDGPLLEECGLDC 329
PF + GP ++ CG++C
Sbjct: 182 PFTTRVAGPNIQTCGINC 235
Score = 100 bits (214), Expect = 2e-022
Identities = 44/61 (72%), Positives = 47/61 (77%)
Frame = -1 / -3
Query: 277 GSIRTQRSEKIEASRMDQTMTIVGVLFCLPTKPLRKGYRWTITQNAKKSFPKRGPHDSYP 98
GSIRT EKIEA R+ QTMTIVGV F LP +PLR GY+ TITQ AKKSFPK GPHD P
Sbjct: 183 GSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TITQKAKKSFPKSGPHDW*P 4
Query: 97 L 95
L
Sbjct: 3 L 1
Score = 29.5 bits (58), Expect(2) = 6e-044
Identities = 8/16 (50%), Positives = 12/16 (75%)
Frame = +3 / +1
Query: 477 PNIWDTLEGKWRLRQS 524
PN+WD L GKW + ++
Sbjct: 214 PNLWDQLLGKWEIAET 261
>gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_A01_A02, mRNA sequence
Length = 474
Score = 137 bits (294), Expect = 2e-033
Identities = 56/69 (81%), Positives = 62/69 (89%)
Frame = +3 / +3
Query: 150 VIVHLYPFLKGLVGRQNRTPTIVIVWSILLASIFSLLWVRIDPFLAKDDGPLLEECGLDC 329
VIVHLYPFLKGL+GRQNRTPTIVIVWSILLASI SLLWVR++PFLAK DGP LEECGLDC
Sbjct: 12 VIVHLYPFLKGLLGRQNRTPTIVIVWSILLASIISLLWVRVNPFLAKTDGPTLEECGLDC 191
Query: 330 N*GMSAHQL 356
* ++ Q+
Sbjct: 192 A**ITVQQM 218
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,714,113
Number of Sequences: 8143
Number of extensions: 136193
Number of successful extensions: 1434
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 219361099
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)