TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1804904.2.2
(798 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819453.1|CN819453 HRO4406_A10_A20ZS5 Lib AA069E1X Aven... 252 5e-068
>gb|CN819453.1|CN819453 HRO4406_A10_A20ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4406_A10_A20, mRNA
sequence
Length = 567
Score = 252 bits (545), Expect = 5e-068
Identities = 109/117 (93%), Positives = 112/117 (95%)
Frame = +3 / +1
Query: 141 YGRLVVIVDVVDQNRALVDAPDMVRCQVNFKRLSLTDIKIDIKRVPKKTTLIKAMEEADV 320
YGRLVVIVDVVDQNRALVDAPDMVRCQ+NFKRLSLTDIKIDIKRVPKK TLIKAMEEADV
Sbjct: 1 YGRLVVIVDVVDQNRALVDAPDMVRCQINFKRLSLTDIKIDIKRVPKKATLIKAMEEADV 180
Query: 321 KTKWENSSWGKKLIVQKRRASLNDFDRFKVMLAKIKRGGAIRQELAKLKKASTA*GV 491
K+KWENSSWGKKLIVQKRRASLNDFDRFKVMLAKIKRGGAIRQELAKLKK A* +
Sbjct: 181 KSKWENSSWGKKLIVQKRRASLNDFDRFKVMLAKIKRGGAIRQELAKLKKEVAA*NI 351
Score = 204 bits (440), Expect = 1e-053
Identities = 91/114 (79%), Positives = 95/114 (83%)
Frame = -3 / -2
Query: 484 *AVDAFLSLASSCLIAPPLLIFASMTLNLSKSLSDALLFWTISFLPHELFSHLVFTSASS 305
*A +FLSLASSCLIAPPLLI A+MTLNLSKS SDALLFWTISFLPHELFSHL+FTSASS
Sbjct: 344 *AATSFLSLASSCLIAPPLLILANMTLNLSKSFSDALLFWTISFLPHELFSHLLFTSASS 165
Query: 304 IALIRVVFLGTRLMSILMSVSESRLKFTWHLTISGASTSALFWXXXXXXXXXRP 143
IALI+V FLGTRLMSILMSV E RLK WHLTISGASTSA FW RP
Sbjct: 164 IALIKVAFLGTRLMSILMSVRERRLKLIWHLTISGASTSARFWSTTSTMTTRRP 3
Score = 174 bits (375), Expect = 1e-044
Identities = 77/110 (70%), Positives = 85/110 (77%)
Frame = +2 / +3
Query: 143 WPSRRHRRCGRPEQGTCGCP*YGQVPGELQAALTY*HQD*HQTCPQEDNPDQGDGGS*CE 322
WP R HRR RPE GTCGC YGQVP LQA+ + *H D HQ CPQE N DQG+GGS*CE
Sbjct: 3 WPPRGHRRRRRPEPGTCGCSRYGQVPD*LQASFSD*H*DRHQACPQEGNFDQGNGGS*CE 182
Query: 323 DQVGEQLMGQEADCPEEESIAQ*L**VQSHAGED*EGWCYQARARQA*EG 472
+QVGEQL+GQEA CPEE+S+A+*L* VQ H G D*E CYQARA QA EG
Sbjct: 183 EQVGEQLVGQEAYCPEEKSVAE*L*QVQGHVG*D*ERRCYQARACQAQEG 332
Score = 170 bits (366), Expect = 2e-043
Identities = 75/118 (63%), Positives = 87/118 (73%)
Frame = -2 / -1
Query: 494 RDSLSCGRLLKLGELLPDSTTPLNLRQHDFEPIKVIERCSPLLDNQXXXXXXXXXXXLHI 315
+D LS LL+LG+LLPDST LNL QHD EP+KVI+R S LLDN+ LHI
Sbjct: 354 KDILSSNLLLELGKLLPDSTASLNLSQHDLEPVKVIQRRSSLLDNKLLAPRAVLPLALHI 175
Query: 314 SFLHRLDQGCLLGDTFDVNLDVSK*EPLEVHLAPDHIRGIHKCPVLVDHIDDDDETAI 141
SFLH LDQ CLLGDT DV+L+VS+ + LEV+LAPDHI IHKCPVLVD +DDD E AI
Sbjct: 174 SFLHCLDQSCLLGDTLDVDLNVSQRKTLEVNLAPDHIGSIHKCPVLVDDVDDDHEAAI 1
Score = 133 bits (284), Expect = 5e-032
Identities = 71/118 (60%), Positives = 74/118 (62%)
Frame = +1 / +2
Query: 142 MAVXXXXXMWSTRTGHLWMPLIWSGAR*TSSGSHLLTSRLTSNVXXXXXXXXXXXXKLM* 321
MA STRTGHLWM IWSGAR TSS LT R TS+V KLM*
Sbjct: 2 MAASWSSSTSSTRTGHLWMLPIWSGARLTSSVFL*LTLRSTSSVSPRRQL*SRQWRKLM* 181
Query: 322 RPSGRTAHGARS*LSRRGEHRSMTLIGSKSCWRRLRGVVLSGKSSPSLRRRPQLKESL 495
R SGRTA GARS LSRR E R MTL GS+SCW RLR VLSGKS PS RRR L+ SL
Sbjct: 182 RASGRTARGARSLLSRREERR*MTLTGSRSCWLRLREAVLSGKSLPSSRRRLLLRISL 355
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,227,146
Number of Sequences: 8143
Number of extensions: 111182
Number of successful extensions: 1055
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 270249036
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)