TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716365.2.1
(936 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819189.1|CN819189 HRO4451_B10_C19ZS5 Lib AA070E1X Aven... 175 1e-044
gb|CN820364.1|CN820364 HRO4418_E06_I12ZS5 Lib AA069E1X Aven... 175 1e-044
gb|AF033096.1|AF033096 Avena sativa nonphototropic hypocoty... 170 4e-043
gb|AF033097.1|AF033097 Avena sativa nonphototropic hypocoty... 170 4e-043
gb|CN819412.1|CN819412 HRO4405_G02_M03ZS5 Lib AA069E1X Aven... 137 5e-041
gb|CN820184.1|CN820184 HRO4412_B04_D08ZS5 Lib AA069E1X Aven... 155 1e-038
>gb|CN819189.1|CN819189 HRO4451_B10_C19ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4451_B10_C19, mRNA sequence
Length = 791
Score = 175 bits (376), Expect = 1e-044
Identities = 64/125 (51%), Positives = 88/125 (70%)
Frame = -3 / +3
Query: 715 SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQP 536
S SFVGT EY+APE+I G GH SA+DWW GI LYE+L+G+TPF+G + T +N++ +
Sbjct: 30 SNSFVGTEEYIAPEVITGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRKKTFYNILHKD 209
Query: 535 LRFPESPVVSFAARDLIRGLLIKEPQHRLAYKRGATEIKQHPFFEGVNWALIRCATPPDI 356
L FP S VS A+ LI GLL ++P R+ GA +IK+HPFFE + W LIRC +PP++
Sbjct: 210 LTFPSSIPVSLEAKQLIHGLLQRDPSARIGSNTGANDIKEHPFFEDIYWPLIRCMSPPEL 389
Query: 355 PKPVE 341
P++
Sbjct: 390 DVPLK 404
Score = 105 bits (224), Expect = 1e-023
Identities = 54/123 (43%), Positives = 65/123 (52%)
Frame = +3 / -2
Query: 348 GLGISGGVAHLINAQLTPSKNGCCFISVAPRLYANRC*GSLMSNPLIRSLAAKLTTGDSG 527
G SGG+ I+ Q SKNGC IS AP R GSL S+P I A++LT G
Sbjct: 397 GTSSSGGLMQRISGQ*ISSKNGCSLISFAPVFEPMRAEGSL*SSPWINCFASRLTGMLLG 218
Query: 528 NLKGCPTTLNNVALFPEPLNGVLPKRSSYKNMPNVHQSTALPCPSPFIISGAKYSWVPTK 707
N++ L NV P GV P SY +P HQS AL CP+P + SGA YS VPT
Sbjct: 217 NVRSLCRML*NVFFLFFPRKGVRPYNISYNRIPRAHQSMALVCPAPVMTSGAMYSSVPTN 38
Query: 708 DID 716
+ D
Sbjct: 37 EFD 29
>gb|CN820364.1|CN820364 HRO4418_E06_I12ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4418_E06_I12, mRNA
sequence
Length = 699
Score = 175 bits (376), Expect = 1e-044
Identities = 64/125 (51%), Positives = 88/125 (70%)
Frame = -3 / +3
Query: 715 SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQP 536
S SFVGT EY+APE+I G GH SA+DWW GI LYE+L+G+TPF+G + T +N++ +
Sbjct: 39 SNSFVGTEEYIAPEVITGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRKKTFYNILHKD 218
Query: 535 LRFPESPVVSFAARDLIRGLLIKEPQHRLAYKRGATEIKQHPFFEGVNWALIRCATPPDI 356
L FP S VS A+ LI GLL ++P R+ GA +IK+HPFFE + W LIRC +PP++
Sbjct: 219 LTFPSSIPVSLEAKQLIHGLLQRDPSARIGSNTGANDIKEHPFFEDIYWPLIRCMSPPEL 398
Query: 355 PKPVE 341
P++
Sbjct: 399 DVPLK 413
Score = 105 bits (224), Expect = 1e-023
Identities = 54/123 (43%), Positives = 65/123 (52%)
Frame = +3 / -3
Query: 348 GLGISGGVAHLINAQLTPSKNGCCFISVAPRLYANRC*GSLMSNPLIRSLAAKLTTGDSG 527
G SGG+ I+ Q SKNGC IS AP R GSL S+P I A++LT G
Sbjct: 406 GTSSSGGLMQRISGQ*ISSKNGCSLISFAPVFEPMRAEGSL*SSPWINCFASRLTGMLLG 227
Query: 528 NLKGCPTTLNNVALFPEPLNGVLPKRSSYKNMPNVHQSTALPCPSPFIISGAKYSWVPTK 707
N++ L NV P GV P SY +P HQS AL CP+P + SGA YS VPT
Sbjct: 226 NVRSLCRML*NVFFLFFPRKGVRPYNISYNRIPRAHQSMALVCPAPVMTSGAMYSSVPTN 47
Query: 708 DID 716
+ D
Sbjct: 46 EFD 38
>gb|AF033096.1|AF033096 Avena sativa nonphototropic hypocotyl 1 (NPH1-1) mRNA, complete cds
Length = 3467
Score = 170 bits (365), Expect = 4e-043
Identities = 67/124 (54%), Positives = 85/124 (68%)
Frame = -3 / +2
Query: 715 SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQP 536
S SFVGT EY+APEII G GH SAVDWW GI LYE+L+G TPF+G + T N++ +
Sbjct: 2735 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 2914
Query: 535 LRFPESPVVSFAARDLIRGLLIKEPQHRLAYKRGATEIKQHPFFEGVNWALIRCATPPDI 356
+RFP S VS AR LI LL ++P +RL G+ EIK+HPFF G+NWAL+R PP +
Sbjct: 2915 IRFPASISVSLPARQLIYRLLHRDPSNRLGSYEGSNEIKEHPFFRGINWALVRGTAPPKL 3094
Query: 355 PKPV 344
P+
Sbjct: 3095 DAPL 3106
Score = 106 bits (227), Expect = 4e-024
Identities = 55/125 (44%), Positives = 68/125 (54%)
Frame = +3 / -3
Query: 342 STGLGISGGVAHLINAQLTPSKNGCCFISVAPRLYANRC*GSLMSNPLIRSLAAKLTTGD 521
++G GG AQL P K GC FIS P + GSL S+ I LA +LT
Sbjct: 3108 NSGASSFGGAVPRTRAQLMPRKKGCSFISFDPSYDPSLFEGSLCSSLYISCLAGRLTDIL 2929
Query: 522 SGNLKGCPTTLNNVALFPEPLNGVLPKRSSYKNMPNVHQSTALPCPSPFIISGAKYSWVP 701
+GNL L NV PL GV P SY+ +P+ HQSTAL P+P +ISGA YS VP
Sbjct: 2928 AGNLMSLCRMLANVLCLVLPLKGV*PYNISYRRIPSAHQSTALVWPAPVMISGAMYSSVP 2749
Query: 702 TKDID 716
T ++D
Sbjct: 2748 TNELD 2734
>gb|AF033097.1|AF033097 Avena sativa nonphototropic hypocotyl 1 (NPH1-2) mRNA, complete cds
Length = 3422
Score = 170 bits (365), Expect = 4e-043
Identities = 67/124 (54%), Positives = 85/124 (68%)
Frame = -3 / +2
Query: 715 SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQP 536
S SFVGT EY+APEII G GH SAVDWW GI LYE+L+G TPF+G + T N++ +
Sbjct: 2672 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 2851
Query: 535 LRFPESPVVSFAARDLIRGLLIKEPQHRLAYKRGATEIKQHPFFEGVNWALIRCATPPDI 356
+RFP S VS AR LI LL ++P +RL G+ EIK+HPFF G+NWAL+R PP +
Sbjct: 2852 IRFPASISVSLPARQLIYRLLHRDPSNRLGSYEGSNEIKEHPFFRGINWALVRGTAPPKL 3031
Query: 355 PKPV 344
P+
Sbjct: 3032 DAPL 3043
Score = 109 bits (233), Expect = 6e-025
Identities = 56/125 (44%), Positives = 69/125 (55%)
Frame = +3 / -3
Query: 342 STGLGISGGVAHLINAQLTPSKNGCCFISVAPRLYANRC*GSLMSNPLIRSLAAKLTTGD 521
++G GG AQL P KNGC FIS P + GSL S+ I LA +LT
Sbjct: 3045 NSGASSLGGAVPRTRAQLMPRKNGCSFISFDPSYDPSLFEGSLCSSLYISCLAGRLTDIL 2866
Query: 522 SGNLKGCPTTLNNVALFPEPLNGVLPKRSSYKNMPNVHQSTALPCPSPFIISGAKYSWVP 701
+GNL L NV PL GV P SY+ +P+ HQSTAL P+P +ISGA YS VP
Sbjct: 2865 AGNLMSLCRMLANVLCLVLPLKGV*PYNISYRRIPSAHQSTALVWPAPVMISGAMYSSVP 2686
Query: 702 TKDID 716
T ++D
Sbjct: 2685 TNELD 2671
>gb|CN819412.1|CN819412 HRO4405_G02_M03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4405_G02_M03, mRNA
sequence
Length = 544
Score = 137 bits (294), Expect(5) = 5e-041
Identities = 55/60 (91%), Positives = 57/60 (95%)
Frame = -3 / +3
Query: 457 HRLAYKRGATEIKQHPFFEGVNWALIRCATPPDIPKPVEIPRSVASSSQKATSAAEKGSD 278
HRLAYKRGATEIKQHPFFEGVNWALIRCATPPDIPKPVEIPR VAS+SQKAT +AEKG D
Sbjct: 63 HRLAYKRGATEIKQHPFFEGVNWALIRCATPPDIPKPVEIPRPVASTSQKATPSAEKGPD 242
Score = 122 bits (260), Expect = 1e-028
Identities = 53/60 (88%), Positives = 53/60 (88%)
Frame = +3 / -1
Query: 279 SEPFSAADVAFWLDEATERGISTGLGISGGVAHLINAQLTPSKNGCCFISVAPRLYANRC 458
S PFSA VAF L EAT RGISTGLGISGGVAHLINAQLTPSK GCCFISVAPRLYANRC
Sbjct: 241 SGPFSADGVAF*LVEATGRGISTGLGISGGVAHLINAQLTPSKKGCCFISVAPRLYANRC 62
Score = 27.2 bits (53), Expect(5) = 5e-041
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = -3 / +2
Query: 517 PVVSFAARDLIRGLLIK 467
P F RDLIRGLLI+
Sbjct: 5 PCRGFCCRDLIRGLLIR 55
Score = 25.8 bits (50), Expect(5) = 5e-041
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = -3 / +3
Query: 190 FLWVLITLYVDAYC 149
F W LIT YVD C
Sbjct: 327 FFWFLIT*YVDGIC 368
Score = 23.1 bits (44), Expect(5) = 5e-041
Identities = 8/10 (80%), Positives = 8/10 (80%)
Frame = -2 / +1
Query: 233 CASCKALPFP 204
CASCK L FP
Sbjct: 292 CASCKVLFFP 321
Score = 22.6 bits (43), Expect(5) = 5e-041
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = -2 / +1
Query: 170 FICRCILSYVVLFSSVDFSVHSFRWDPQSGGVCNPL 63
++ IL + + F S S W+P SG CN L
Sbjct: 364 YVPSAILMFHLQFISPH*SQWICGWEPLSGVCCNIL 471
>gb|CN820184.1|CN820184 HRO4412_B04_D08ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4412_B04_D08, mRNA
sequence
Length = 610
Score = 155 bits (332), Expect = 1e-038
Identities = 61/109 (55%), Positives = 76/109 (69%)
Frame = -3 / +2
Query: 715 SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQP 536
S SFVGT EY+APEII G GH SAVDWW GI LYE+L+G TPF+G + T N++ +
Sbjct: 176 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 355
Query: 535 LRFPESPVVSFAARDLIRGLLIKEPQHRLAYKRGATEIKQHPFFEGVNW 389
+RFP S VS AR LI LL ++P +RL G+ EIK+HPFF G+NW
Sbjct: 356 IRFPASISVSLPARQLIYRLLHRDPSNRLGSHEGSNEIKEHPFFRGINW 502
Score = 101 bits (216), Expect = 1e-022
Identities = 52/114 (45%), Positives = 63/114 (55%)
Frame = +3 / -2
Query: 375 HLINAQLTPSKNGCCFISVAPRLYANRC*GSLMSNPLIRSLAAKLTTGDSGNLKGCPTTL 554
H QL P K GC FIS P + GSL S+ I LA +LT +GNL L
Sbjct: 516 HAREPQLMPRKKGCSFISFDPSCDPSLFEGSLCSSLYISCLAGRLTDILAGNLMSLCRML 337
Query: 555 NNVALFPEPLNGVLPKRSSYKNMPNVHQSTALPCPSPFIISGAKYSWVPTKDID 716
NV PL GV P SY+ +P+ HQSTAL P+P +ISGA YS VPT ++D
Sbjct: 336 ANVLCLVLPLKGV*PYNISYRRIPSAHQSTALVWPAPVMISGAMYSSVPTNELD 175
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,433,356
Number of Sequences: 8143
Number of extensions: 166038
Number of successful extensions: 1101
Number of sequences better than 1.0e-020: 7
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 326246584
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)