TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131584.2.3
(687 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818609.1|CN818609 HRO4467_C02_E03ZS5 Lib AA070E1X Aven... 182 4e-047
gb|CN818166.1|CN818166 HRO4471_C12_E23ZS5 Lib AA070E1X Aven... 177 2e-045
>gb|CN818609.1|CN818609 HRO4467_C02_E03ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4467_C02_E03, mRNA sequence
Length = 463
Score = 182 bits (393), Expect = 4e-047
Identities = 76/76 (100%), Positives = 76/76 (100%)
Frame = -1 / +2
Query: 519 TQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKM 340
TQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKM
Sbjct: 2 TQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKM 181
Query: 339 FLRGDSVIIVLRNPK* 292
FLRGDSVIIVLRNPK*
Sbjct: 182 FLRGDSVIIVLRNPK* 229
Score = 141 bits (303), Expect = 1e-034
Identities = 64/80 (80%), Positives = 66/80 (82%)
Frame = +2 / -2
Query: 281 RLGDHLGFLSTMMTESPLRNILLMNLSLFTGRAFFLPLPVLGTSVHISRTFSSTMLQWRS 460
RL H GFL TMMTESP RNILLMNLSLFTGRAFFLPLPV GTSVHIS TFS TMLQ RS
Sbjct: 240 RLQTHFGFLRTMMTESPRRNILLMNLSLFTGRAFFLPLPVFGTSVHISLTFSRTMLQ*RS 61
Query: 461 KARTRPSSFLLLRQLMRTWV 520
A TRPS+FLL RQL+ T V
Sbjct: 60 NALTRPSNFLLFRQLISTCV 1
Score = 107 bits (228), Expect = 2e-024
Identities = 44/76 (57%), Positives = 54/76 (71%)
Frame = -3 / +1
Query: 520 HPGSHQLPQQQEAAWPGSCF*SPL*HGARERAGDVDRGPQDWQGQKEGSACEQG*IHQQD 341
H G++QLP+QQE WP S L HG+ ER GDVD GP+DWQ Q+EGS+ EQG +HQQD
Sbjct: 1 HTGAYQLPEQQEVTWPREGIRSSLQHGS*ER*GDVD*GPKDWQRQEEGSSSEQGQVHQQD 180
Query: 340 VPQGRLGHHRAEEPQV 293
VP L HH +EP+V
Sbjct: 181 VPPRGLCHHCPQEPKV 228
Score = 104 bits (221), Expect = 2e-023
Identities = 46/75 (61%), Positives = 58/75 (77%)
Frame = +1 / -1
Query: 292 SLGVPQHDDDRVAPEEHLADESILVHRQSLLFALASLGDLCPHLPHVLEHHVTVAIKSTN 471
SL VP+ +DDRV EEHLADE +LVH +SLL A ASL DL PHLP+VL++HV V I+ +
Sbjct: 229 SLWVPEDNDDRVPAEEHLADEPVLVHWKSLLLAFASLWDLSPHLPNVLKNHVAVTIECPH 50
Query: 472 PAKQLLVVAAIDENL 516
AK LLVV A+D++L
Sbjct: 49 AAK*LLVVPAVDKHL 5
>gb|CN818166.1|CN818166 HRO4471_C12_E23ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4471_C12_E23, mRNA sequence
Length = 475
Score = 177 bits (381), Expect = 2e-045
Identities = 74/77 (96%), Positives = 75/77 (97%)
Frame = -1 / +2
Query: 510 LINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKMFLR 331
LINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKMFLR
Sbjct: 2 LINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKMFLR 181
Query: 330 GDSVIIVLRNPK*SPNR 280
GDSVIIVLRNPK*+ R
Sbjct: 182 GDSVIIVLRNPK*A*GR 232
Score = 141 bits (302), Expect = 1e-034
Identities = 60/72 (83%), Positives = 62/72 (86%)
Frame = +2 / -2
Query: 293 HLGFLSTMMTESPLRNILLMNLSLFTGRAFFLPLPVLGTSVHISRTFSSTMLQWRSKART 472
H GFL TMMTESP RNILLMNLSLFTGRAFFLP PV GTSVHIS TFS TMLQWRS A T
Sbjct: 219 HFGFLRTMMTESPRRNILLMNLSLFTGRAFFLPFPVFGTSVHISLTFSRTMLQWRSNALT 40
Query: 473 RPSSFLLLRQLM 508
RPS+FLL RQL+
Sbjct: 39 RPSNFLLFRQLI 4
Score = 121 bits (258), Expect = 2e-028
Identities = 47/73 (64%), Positives = 58/73 (79%)
Frame = -3 / +1
Query: 511 SHQLPQQQEAAWPGSCF*SPL*HGARERAGDVDRGPQDWQGQKEGSACEQG*IHQQDVPQ 332
++QLP+QQE WP *SPL HG+ ER GDVDRG +DW+ Q+EGS+CEQG +HQQDVP
Sbjct: 1 AYQLPEQQEVTWPREGI*SPLQHGS*ER*GDVDRGSKDWERQEEGSSCEQGQVHQQDVPP 180
Query: 331 GRLGHHRAEEPQV 293
RLGHH +EEP+V
Sbjct: 181 RRLGHHCSEEPEV 219
Score = 108 bits (230), Expect = 1e-024
Identities = 48/77 (62%), Positives = 60/77 (77%)
Frame = +1 / -1
Query: 280 PVGRSLGVPQHDDDRVAPEEHLADESILVHRQSLLFALASLGDLCPHLPHVLEHHVTVAI 459
P SL VPQ++DDRV+ EEHLADE +LVHR+SLL A SL +L PHLP+VL++HV VAI
Sbjct: 232 PSSGSLRVPQNNDDRVSAEEHLADEPVLVHRKSLLLAFPSLWNLGPHLPNVLKNHVAVAI 53
Query: 460 KSTNPAKQLLVVAAIDE 510
K + AK LLVV A+D+
Sbjct: 52 KCPHAAK*LLVVPAVDK 2
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,817,196
Number of Sequences: 8143
Number of extensions: 111066
Number of successful extensions: 1218
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 227939466
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)