TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131555.2.3
(622 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819665.1|CN819665 HRO4402_G05_M10ZS5 Lib AA069E1X Aven... 214 1e-056
gb|CN820523.1|CN820523 HRO4413_D02_G03ZS5 Lib AA069E1X Aven... 210 2e-055
gb|CN818757.1|CN818757 HRO4468_C07_E14ZS5 Lib AA070E1X Aven... 114 2e-026
>gb|CN819665.1|CN819665 HRO4402_G05_M10ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4402_G05_M10, mRNA
sequence
Length = 632
Score = 214 bits (462), Expect = 1e-056
Identities = 87/123 (70%), Positives = 106/123 (86%)
Frame = -3 / +2
Query: 620 SSKIILEYFTGDRETVENILRNMVDVRDVADALLLAYEKPEASGRYICSSHAIKVADMIN 441
SSK++L YF G+ ETVEN RN+VDVRDVADA+LLAYE EASGRYICSS IK +D+I+
Sbjct: 104 SSKVLLSYFKGEHETVENKYRNLVDVRDVADAILLAYENAEASGRYICSSSPIKASDLIS 283
Query: 440 ILKTLYPSYPYPKNFVEDDVNSVYSSEKLQKLGWSFKPIEESLRDTVESYKAFGILN*TS 261
ILKTLYP+YPYPKNF E + ++ YSSEKLQKLGW+F+PIEE+LRD+VESY+A G+LN* S
Sbjct: 284 ILKTLYPTYPYPKNFAEVEHSTTYSSEKLQKLGWTFRPIEETLRDSVESYRASGVLN*AS 463
Query: 260 SYD 252
+ D
Sbjct: 464 NRD 472
Score = 150 bits (322), Expect = 2e-037
Identities = 66/123 (53%), Positives = 82/123 (66%)
Frame = +3 / -2
Query: 252 VVAGCSIQDAKCFVGFNSVAEGFLYRLEAPPQLLQFLRAVYRVDIIFHKIFGIRVRWIQG 431
V GCS+QDA+C VG ++V EGFLY E PPQ L+FLR V F K+FGIRVRWI G
Sbjct: 472 VSIGCSVQDARCSVGLDAVPEGFLYGPEGPPQFLKFLRTVSGTVFHFCKVFGIRVRWI*G 293
Query: 432 LQYVYHISNFYCM*TADVSSRRFWLFVSQKKSVSNITNIHHISEYVFNSLAISCEIFKDD 611
LQY Y I F TA V+SRR +F+ QK S+ NI+NIH + ++F SL S +I K+D
Sbjct: 292 LQYAYQIRGFNW*RTAYVASRRLCIFICQKNSIGNISNIH*VPVFIFYSLVFSFKITKED 113
Query: 612 FAA 620
F A
Sbjct: 112 FTA 104
Score = 134 bits (288), Expect = 1e-032
Identities = 71/124 (57%), Positives = 78/124 (62%)
Frame = -1 / +3
Query: 622 RAAKSSLNISQEIARLLKTYSEIWWMFVMLLTLFFWLTKSQKRLDDTSAVHMQ*KLLI** 443
RA KSSL I +E RL K + WMF ML LFFW K Q+RLD T AV Q K LI**
Sbjct: 102 RAVKSSLVILKENTRL*KINTGT*WMFEMLPMLFFWHMKMQRRLDATYAVLHQLKPLI** 281
Query: 442 TY*RPCIHLTLIPKILWKMMSTLYTALRNCRSWGGASSL*RNPSATLLNPTKHLAS*IEH 263
Y*RP I TLIPK L K + T LRN R+WGG S *R PS T +PT+HLAS* EH
Sbjct: 282 AY*RPYIQRTLIPKTLQKWNTVPLTVLRNFRNWGGPSGP*RKPSGTASSPTEHLAS*TEH 461
Query: 262 PATT 251
P T
Sbjct: 462 PIET 473
Score = 113 bits (241), Expect = 3e-026
Identities = 50/85 (58%), Positives = 60/85 (70%)
Frame = -2 / +1
Query: 507 KARSVWTIHLQFTCNKSC*YDKHTEDLVSILPLSQKFCGR*CQLCIQL*ETAEAGVELQA 328
K R VWT+H+QF N S * DKHTEDL+S +PLSQK C Q +Q *ET+E GV+LQA
Sbjct: 217 KCRGVWTLHMQFFTN*SL*SDKHTEDLISNVPLSQKLCRSGTQYHLQF*ETSETGVDLQA 396
Query: 327 YRGIPPRHC*ILQSIWHPELNIQLR 253
+RG P +LQSIW PEL+IQ R
Sbjct: 397 HRGNPQGQRRVLQSIWRPELSIQSR 471
Score = 72.5 bits (152), Expect(2) = 3e-025
Identities = 39/75 (52%), Positives = 41/75 (54%)
Frame = +1 / -3
Query: 397 KFLG*G*DGYKVFSMFIISATFIACELQMYRPDASGFXXXXXXXXXXXXXXXIFLSMFST 576
KFLG*G GYKVFSM I S I EL M RPDAS F FL +FST
Sbjct: 327 KFLG*GYVGYKVFSMLIRSEALIGEELHM*RPDASAFSYAKRIASATSRTSTRFLYLFST 148
Query: 577 VSRSPVKYSRMILLL 621
VS SP+K R LLL
Sbjct: 147 VSCSPLK*LRRTLLL 103
Score = 57.0 bits (118), Expect(2) = 3e-025
Identities = 25/38 (65%), Positives = 31/38 (81%)
Frame = +1 / -3
Query: 238 QQMEQS*LDVQFRMPNAL*DSTVSRRDSSIGLKLHPSF 351
Q++ S LD QFR P+AL*DST+S R SS+GLK+HPSF
Sbjct: 486 QRLNLSRLDAQFRTPDAL*DSTLSLRVSSMGLKVHPSF 373
>gb|CN820523.1|CN820523 HRO4413_D02_G03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4413_D02_G03, mRNA
sequence
Length = 704
Score = 210 bits (453), Expect = 2e-055
Identities = 85/118 (72%), Positives = 103/118 (87%)
Frame = -3 / +3
Query: 620 SSKIILEYFTGDRETVENILRNMVDVRDVADALLLAYEKPEASGRYICSSHAIKVADMIN 441
SSK++L YF G+ ETVEN RN+VDVRDVADA+LLAYE EASGRYICSS IK +D+I+
Sbjct: 348 SSKVLLSYFKGEHETVENKYRNLVDVRDVADAILLAYENAEASGRYICSSSPIKASDLIS 527
Query: 440 ILKTLYPSYPYPKNFVEDDVNSVYSSEKLQKLGWSFKPIEESLRDTVESYKAFGILN* 267
ILKTLYP+YPYPKNF E + ++ YSSEKLQKLGW+F+PIEE+LRD+VESY+A G+LN*
Sbjct: 528 ILKTLYPTYPYPKNFAEVEHSTTYSSEKLQKLGWTFRPIEETLRDSVESYRASGVLN* 701
Score = 147 bits (315), Expect = 2e-036
Identities = 64/119 (53%), Positives = 80/119 (67%)
Frame = +3 / -1
Query: 264 CSIQDAKCFVGFNSVAEGFLYRLEAPPQLLQFLRAVYRVDIIFHKIFGIRVRWIQGLQYV 443
CS+QDA+C VG ++V EGFLY E PPQ L+FLR V F K+FGIRVRWI GLQY
Sbjct: 704 CSVQDARCSVGLDAVPEGFLYGPEGPPQFLKFLRTVSGTVFHFCKVFGIRVRWI*GLQYA 525
Query: 444 YHISNFYCM*TADVSSRRFWLFVSQKKSVSNITNIHHISEYVFNSLAISCEIFKDDFAA 620
Y I F TA V+SRR +F+ QK S+ NI+NIH + ++F SL S +I K+DF A
Sbjct: 524 YQIRGFNW*RTAYVASRRLCIFICQKNSIGNISNIH*VPVFIFYSLVFSFKITKEDFTA 348
Score = 126 bits (270), Expect = 3e-030
Identities = 68/119 (57%), Positives = 75/119 (63%)
Frame = -1 / +1
Query: 622 RAAKSSLNISQEIARLLKTYSEIWWMFVMLLTLFFWLTKSQKRLDDTSAVHMQ*KLLI** 443
RA KSSL I +E RL K + WMF ML LFFW K Q+RLD T AV Q K LI**
Sbjct: 346 RAVKSSLVILKENTRL*KINTGT*WMFEMLPMLFFWHMKMQRRLDATYAVLHQLKPLI** 525
Query: 442 TY*RPCIHLTLIPKILWKMMSTLYTALRNCRSWGGASSL*RNPSATLLNPTKHLAS*IE 266
Y*RP I TLIPK L K + T LRN R+WGG S *R PS T +PT+HLAS* E
Sbjct: 526 AY*RPYIQRTLIPKTLQKWNTVPLTVLRNFRNWGGPSGP*RKPSGTASSPTEHLAS*TE 702
Score = 107 bits (229), Expect = 1e-024
Identities = 47/81 (58%), Positives = 57/81 (70%)
Frame = -2 / +2
Query: 507 KARSVWTIHLQFTCNKSC*YDKHTEDLVSILPLSQKFCGR*CQLCIQL*ETAEAGVELQA 328
K R VWT+H+QF N S * DKHTEDL+S +PLSQK C Q +Q *ET+E GV+LQA
Sbjct: 461 KCRGVWTLHMQFFTN*SL*SDKHTEDLISNVPLSQKLCRSGTQYHLQF*ETSETGVDLQA 640
Query: 327 YRGIPPRHC*ILQSIWHPELN 265
+RG P +LQSIW PEL+
Sbjct: 641 HRGNPQGQRRVLQSIWRPELS 703
Score = 72.5 bits (152), Expect(2) = 4e-023
Identities = 39/75 (52%), Positives = 41/75 (54%)
Frame = +1 / -2
Query: 397 KFLG*G*DGYKVFSMFIISATFIACELQMYRPDASGFXXXXXXXXXXXXXXXIFLSMFST 576
KFLG*G GYKVFSM I S I EL M RPDAS F FL +FST
Sbjct: 571 KFLG*GYVGYKVFSMLIRSEALIGEELHM*RPDASAFSYAKRIASATSRTSTRFLYLFST 392
Query: 577 VSRSPVKYSRMILLL 621
VS SP+K R LLL
Sbjct: 391 VSCSPLK*LRRTLLL 347
Score = 49.6 bits (102), Expect(2) = 4e-023
Identities = 21/28 (75%), Positives = 25/28 (89%)
Frame = +1 / -2
Query: 268 QFRMPNAL*DSTVSRRDSSIGLKLHPSF 351
QFR P+AL*DST+S R SS+GLK+HPSF
Sbjct: 700 QFRTPDAL*DSTLSLRVSSMGLKVHPSF 617
>gb|CN818757.1|CN818757 HRO4468_C07_E14ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4468_C07_E14, mRNA sequence
Length = 805
Score = 114 bits (243), Expect = 2e-026
Identities = 47/116 (40%), Positives = 74/116 (63%)
Frame = -3 / +3
Query: 620 SSKIILEYFTGDRETVENILRNMVDVRDVADALLLAYEKPEASGRYICSSHAIKVADMIN 441
+S +++ G +T+ N MVDVRDVA+ALLL Y K +S RYICS + + D++
Sbjct: 333 TSNVLIYILKGGPDTMNNKFYPMVDVRDVAEALLLLYNKAGSSERYICSLDQMDLKDLLG 512
Query: 440 ILKTLYPSYPYPKNFVEDDVNSVYSSEKLQKLGWSFKPIEESLRDTVESYKAFGIL 273
ILK +YP+Y V+ D + +S+KL+ LGW + +EE+L D+++SY+ G+L
Sbjct: 513 ILKNMYPNYRCTDKMVDVDYKVIVTSDKLKNLGWKPRKLEETLEDSIKSYEKAGLL 680
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,372,696
Number of Sequences: 8143
Number of extensions: 98877
Number of successful extensions: 1329
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 200978884
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)