TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131554.2.6
(761 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN814783.1|CN814783 HRO4502_D11_G22ZS5 Lib AA071E1X Aven... 179 4e-046
gb|CN821564.1|CN821564 HRO4426_G05_M10ZS5 Lib AA069E1X Aven... 179 4e-046
gb|CN819743.1|CN819743 HRO4403_F03_L05ZS5 Lib AA069E1X Aven... 157 2e-039
>gb|CN814783.1|CN814783 HRO4502_D11_G22ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_D11_G22, mRNA sequence
Length = 479
Score = 179 bits (386), Expect = 4e-046
Identities = 78/78 (100%), Positives = 78/78 (100%)
Frame = -1 / +2
Query: 620 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 441
VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT
Sbjct: 29 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 208
Query: 440 VTAMDVVYALKRQGRTLY 387
VTAMDVVYALKRQGRTLY
Sbjct: 209 VTAMDVVYALKRQGRTLY 262
Score = 141 bits (302), Expect(2) = 9e-042
Identities = 65/68 (95%), Positives = 65/68 (95%)
Frame = +1 / -3
Query: 409 LSA*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRLTPPLLASLRIAG 588
LSA*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMR TPPL ASLRIAG
Sbjct: 240 LSA*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRFTPPLRASLRIAG 61
Query: 589 LVMPWMLS 612
VMPWMLS
Sbjct: 60 FVMPWMLS 37
Score = 44.1 bits (90), Expect(2) = 9e-042
Identities = 18/20 (90%), Positives = 20/20 (100%)
Frame = +3 / -2
Query: 372 LAAEAVEGAALSLERVDDIH 431
LAAEAVEGAAL+LERVDD+H
Sbjct: 277 LAAEAVEGAALALERVDDVH 218
>gb|CN821564.1|CN821564 HRO4426_G05_M10ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4426_G05_M10, mRNA
sequence
Length = 477
Score = 179 bits (386), Expect = 4e-046
Identities = 78/78 (100%), Positives = 78/78 (100%)
Frame = -1 / +1
Query: 620 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 441
VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT
Sbjct: 58 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 237
Query: 440 VTAMDVVYALKRQGRTLY 387
VTAMDVVYALKRQGRTLY
Sbjct: 238 VTAMDVVYALKRQGRTLY 291
Score = 139 bits (299), Expect(2) = 5e-042
Identities = 64/68 (94%), Positives = 65/68 (95%)
Frame = +1 / -2
Query: 409 LSA*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRLTPPLLASLRIAG 588
LSA*TTSMAVTV RRACSV*VTASRMTFSRKIL TPRVSS*MSPEMR TPPLLASLRIAG
Sbjct: 269 LSA*TTSMAVTVFRRACSV*VTASRMTFSRKILRTPRVSS*MSPEMRFTPPLLASLRIAG 90
Query: 589 LVMPWMLS 612
LVMPW+LS
Sbjct: 89 LVMPWILS 66
Score = 46.4 bits (95), Expect(2) = 5e-042
Identities = 21/36 (58%), Positives = 27/36 (75%)
Frame = +3 / -1
Query: 324 RPPPTQMQFATR*GSDLAAEAVEGAALSLERVDDIH 431
R +Q +T ++LA+EAVEGAALSLERVDD+H
Sbjct: 354 RETTSQQNCSTVSTTNLASEAVEGAALSLERVDDVH 247
>gb|CN819743.1|CN819743 HRO4403_F03_L05ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4403_F03_L05, mRNA
sequence
Length = 343
Score = 157 bits (337), Expect = 2e-039
Identities = 68/68 (100%), Positives = 68/68 (100%)
Frame = -1 / +2
Query: 590 KPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYAL 411
KPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYAL
Sbjct: 5 KPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYAL 184
Query: 410 KRQGRTLY 387
KRQGRTLY
Sbjct: 185 KRQGRTLY 208
Score = 117 bits (250), Expect(2) = 4e-032
Identities = 56/62 (90%), Positives = 57/62 (91%)
Frame = +1 / -2
Query: 409 LSA*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRLTPPLLASLRIAG 588
LSA*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMR TPP A RIAG
Sbjct: 186 LSA*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRFTPPRRARRRIAG 7
Query: 589 LV 594
L+
Sbjct: 6 LM 1
Score = 35.4 bits (71), Expect(2) = 4e-032
Identities = 13/19 (68%), Positives = 18/19 (94%)
Frame = +3 / -1
Query: 375 AAEAVEGAALSLERVDDIH 431
A+EAVEGA+L+LE +DD+H
Sbjct: 220 ASEAVEGASLALECIDDVH 164
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,676,866
Number of Sequences: 8143
Number of extensions: 112137
Number of successful extensions: 2338
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 255640980
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)