TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.8
(708 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815299.1|CN815299 HRO4508_B07_D14ZS5 Lib AA071E1X Aven... 151 1e-037
gb|CN814648.1|CN814648 HRO4501_A03_A05ZS5 Lib AA071E1X Aven... 133 4e-032
gb|CN816820.1|CN816820 HRO4525_B10_C19ZS5 Lib AA071E1X Aven... 124 1e-029
gb|CN820796.1|CN820796 HRO4420_A02_B04ZS5 Lib AA069E1X Aven... 119 4e-028
gb|CN815018.1|CN815018 HRO4505_A01_A01ZS5 Lib AA071E1X Aven... 118 8e-028
gb|CN821631.1|CN821631 HRO4408_E02_J04ZS5 Lib AA069E1X Aven... 101 1e-022
>gb|CN815299.1|CN815299 HRO4508_B07_D14ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_B07_D14, mRNA sequence
Length = 470
Score = 151 bits (324), Expect = 1e-037
Identities = 58/72 (80%), Positives = 67/72 (93%)
Frame = -2 / +1
Query: 545 QKSKMARERNLEKNKGAKGSQLETNKKAMSIQCKVCMQTFMCTTTEVKCREHAEAKHPKT 366
QKSKMARERNLEK KG KGSQLE NKKAM+IQCK+CMQTF+CTT+E KC+EHAEAKHPK+
Sbjct: 7 QKSKMARERNLEKLKGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEAKHPKS 186
Query: 365 DVYQCFPHLKK* 330
++ QCFPHLK+*
Sbjct: 187 ELVQCFPHLKQ* 222
Score = 123 bits (264), Expect = 2e-029
Identities = 49/71 (69%), Positives = 52/71 (73%)
Frame = +1 / -1
Query: 331 HFFRWGKHWYTSVLGCLASACSRHFTSVVVHMNVCMHTLHWMLMAFLLVSSWLPLAPLFF 510
H RWGKHW +S+ GC ASACS HF S VV MNVCMH LHWM MAFLL SS LP PL F
Sbjct: 221 HCLRWGKHWTSSLFGCFASACSLHFASEVVQMNVCMHILHWMFMAFLLASS*LPFPPLSF 42
Query: 511 SKLRSRAILDF 543
S+ S AILDF
Sbjct: 41 SRFLSLAILDF 9
>gb|CN814648.1|CN814648 HRO4501_A03_A05ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4501_A03_A05, mRNA sequence
Length = 391
Score = 133 bits (284), Expect = 4e-032
Identities = 50/65 (76%), Positives = 59/65 (90%)
Frame = -2 / +1
Query: 524 ERNLEKNKGAKGSQLETNKKAMSIQCKVCMQTFMCTTTEVKCREHAEAKHPKTDVYQCFP 345
E NLEK KG KGSQLE NKKAM+IQCK+CMQTF+CTT+E KC+EHAEAKHPK+++ QCFP
Sbjct: 7 EGNLEKLKGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEAKHPKSELVQCFP 186
Query: 344 HLKK* 330
HLK+*
Sbjct: 187 HLKQ* 201
Score = 113 bits (242), Expect = 3e-026
Identities = 44/65 (67%), Positives = 47/65 (72%)
Frame = +1 / -3
Query: 331 HFFRWGKHWYTSVLGCLASACSRHFTSVVVHMNVCMHTLHWMLMAFLLVSSWLPLAPLFF 510
H RWGKHW +S+ GC ASACS HF S VV MNVCMH LHWM MAFLL SS LP PL F
Sbjct: 200 HCLRWGKHWTSSLFGCFASACSLHFASEVVQMNVCMHILHWMFMAFLLASS*LPFPPLSF 21
Query: 511 SKLRS 525
S+ S
Sbjct: 20 SRFPS 6
>gb|CN816820.1|CN816820 HRO4525_B10_C19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4525_B10_C19, mRNA sequence
Length = 416
Score = 124 bits (266), Expect = 1e-029
Identities = 46/60 (76%), Positives = 55/60 (91%)
Frame = -2 / +2
Query: 509 KNKGAKGSQLETNKKAMSIQCKVCMQTFMCTTTEVKCREHAEAKHPKTDVYQCFPHLKK* 330
K KG KGSQLE NKKAM+IQCK+CMQTF+CTT+E KC+EHAEAKHPK+++ QCFPHLK+*
Sbjct: 11 KLKGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEAKHPKSELVQCFPHLKQ* 190
Score = 110 bits (234), Expect = 3e-025
Identities = 42/60 (70%), Positives = 44/60 (73%)
Frame = +1 / -3
Query: 331 HFFRWGKHWYTSVLGCLASACSRHFTSVVVHMNVCMHTLHWMLMAFLLVSSWLPLAPLFF 510
H RWGKHW +S+ GC ASACS HF S VV MNVCMH LHWM MAFLL SS LP PL F
Sbjct: 189 HCLRWGKHWTSSLFGCFASACSLHFASEVVQMNVCMHILHWMFMAFLLASS*LPFPPLSF 10
>gb|CN820796.1|CN820796 HRO4420_A02_B04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4420_A02_B04, mRNA
sequence
Length = 417
Score = 119 bits (255), Expect = 4e-028
Identities = 44/58 (75%), Positives = 53/58 (91%)
Frame = -2 / +3
Query: 503 KGAKGSQLETNKKAMSIQCKVCMQTFMCTTTEVKCREHAEAKHPKTDVYQCFPHLKK* 330
KG KGSQL NKKAM+IQCK+CMQTF+CTT+E KC+EHAEAKHPK+++ QCFPHLK+*
Sbjct: 3 KGGKGSQL*ANKKAMNIQCKICMQTFICTTSEAKCKEHAEAKHPKSELVQCFPHLKQ* 176
Score = 104 bits (222), Expect = 1e-023
Identities = 40/58 (68%), Positives = 42/58 (72%)
Frame = +1 / -3
Query: 331 HFFRWGKHWYTSVLGCLASACSRHFTSVVVHMNVCMHTLHWMLMAFLLVSSWLPLAPL 504
H RWGKHW +S+ GC ASACS HF S VV MNVCMH LHWM MAFLL S LP PL
Sbjct: 175 HCLRWGKHWTSSLFGCFASACSLHFASEVVQMNVCMHILHWMFMAFLLA*S*LPFPPL 2
>gb|CN815018.1|CN815018 HRO4505_A01_A01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4505_A01_A01, mRNA sequence
Length = 338
Score = 118 bits (253), Expect = 8e-028
Identities = 43/55 (78%), Positives = 52/55 (94%)
Frame = -2 / +1
Query: 494 KGSQLETNKKAMSIQCKVCMQTFMCTTTEVKCREHAEAKHPKTDVYQCFPHLKK* 330
KGSQLE NKKAM+IQCK+CMQTF+CTT+E KC+EHAEAKHPK+++ QCFPHLK+*
Sbjct: 4 KGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEAKHPKSELVQCFPHLKQ* 168
Score = 103 bits (220), Expect = 3e-023
Identities = 39/54 (72%), Positives = 41/54 (75%)
Frame = +1 / -1
Query: 331 HFFRWGKHWYTSVLGCLASACSRHFTSVVVHMNVCMHTLHWMLMAFLLVSSWLP 492
H RWGKHW +S+ GC ASACS HF S VV MNVCMH LHWM MAFLL SS LP
Sbjct: 167 HCLRWGKHWTSSLFGCFASACSLHFASEVVQMNVCMHILHWMFMAFLLASS*LP 6
>gb|CN821631.1|CN821631 HRO4408_E02_J04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4408_E02_J04, mRNA
sequence
Length = 357
Score = 101 bits (215), Expect = 1e-022
Identities = 36/47 (76%), Positives = 44/47 (93%)
Frame = -2 / +3
Query: 470 KKAMSIQCKVCMQTFMCTTTEVKCREHAEAKHPKTDVYQCFPHLKK* 330
KKAM IQCK+CMQTF+CTT+E KC+EHAEAKHPK+++ QCFPHLK+*
Sbjct: 12 KKAMYIQCKICMQTFICTTSEAKCKEHAEAKHPKSELVQCFPHLKQ* 152
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,469,300
Number of Sequences: 8143
Number of extensions: 149986
Number of successful extensions: 1882
Number of sequences better than 1.0e-020: 6
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 233728896
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)