TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.707
(919 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN814915.1|CN814915 HRO4503_H05_P09ZS5 Lib AA071E1X Aven... 159 3e-059
gb|CN816851.1|CN816851 HRO4525_E06_I11ZS5 Lib AA071E1X Aven... 156 6e-058
gb|CN816312.1|CN816312 HRO4519_C11_F21ZS5 Lib AA071E1X Aven... 165 1e-041
gb|CN819420.1|CN819420 HRO4405_G10_M19ZS5 Lib AA069E1X Aven... 160 2e-040
>gb|CN814915.1|CN814915 HRO4503_H05_P09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_H05_P09, mRNA sequence
Length = 725
Score = 159 bits (341), Expect(2) = 3e-059
Identities = 69/81 (85%), Positives = 74/81 (91%)
Frame = -1 / +1
Query: 631 AAKDSAVVLSTTKTKKQNAPAKLSHKSVMRKEFRKMAKAVKNQVSDNYYRPDLTKPALAR 452
A K+ VVLSTTKTKKQN PA +HK+VMRKEFRKMAKAVKNQVSDNYYRPDLTKPALAR
Sbjct: 256 ADKEMTVVLSTTKTKKQNKPAAFTHKTVMRKEFRKMAKAVKNQVSDNYYRPDLTKPALAR 435
Query: 451 LSSVYLSLQVAKSGVKKKNRQ 389
LS+V+ SLQVAKSGVKKKNRQ
Sbjct: 436 LSAVHRSLQVAKSGVKKKNRQ 498
Score = 136 bits (292), Expect(2) = 4e-046
Identities = 58/83 (69%), Positives = 63/83 (75%)
Frame = +1 / -2
Query: 382 LRTACSSS*HRTWQPGG*GTPSSA*QELAWSDQACSSCH*PGFSQPWPSCGTPCASQTCG 561
L TACSSS*H+TWQPGG G SA +E AWS QACSSC *PG +PWPSCGTPCA Q+CG
Sbjct: 505 LLTACSSS*HQTWQPGGCGELHSALREQAWSSQACSSCR*PGS*RPWPSCGTPCA*QSCG 326
Query: 562 *ASQVRSASWSW**RGPQPSPLQ 630
* +V SAS SW RGPQ SP Q
Sbjct: 325 *RQRVCSASLSWSWRGPQSSPYQ 257
Score = 132 bits (282), Expect(2) = 1e-049
Identities = 51/78 (65%), Positives = 64/78 (82%)
Frame = -1 / +1
Query: 814 MATVPGDLIWQVVRKNNSFLVKQFGNGNAKVQFTKEPNNLYNIHSFKHSGLANKKTVTIQ 635
MATV L+W++V+KNN FL+KQFGN NAKVQF+KEPNNLYN+HS+K+SGLANKKTVT+Q
Sbjct: 70 MATVSDSLVWELVKKNNCFLIKQFGNSNAKVQFSKEPNNLYNVHSYKYSGLANKKTVTVQ 249
Query: 634 PAAKDSAVVLSTTKTKKQ 581
PAA V+ +T K+
Sbjct: 250 PAADKEMTVVLSTTKTKK 303
Score = 99.6 bits (211), Expect(2) = 5e-032
Identities = 47/77 (61%), Positives = 54/77 (70%)
Frame = +3 / -1
Query: 585 FLVLVVERTTAESFAAGWIVTVFLFAKPECLKEWML*RLFGSLVNCTLALPLPNCFTRKE 764
F+++V T AAG VTVFLFA+PE L EW L*RL GSL+N TLAL LPNC RK+
Sbjct: 299 FVLVVERTTVISLSAAGCTVTVFLFARPEYL*EWTL*RLLGSLLN*TLALLLPNCLIRKQ 120
Query: 765 LFFLTTCQIRSPGTVAI 815
LFFLT+ Q R TVAI
Sbjct: 119 LFFLTSSQTRESETVAI 69
Score = 85.8 bits (181), Expect(2) = 3e-021
Identities = 42/68 (61%), Positives = 47/68 (69%)
Frame = +2 / -3
Query: 428 EAEVHRAQPSKSWLGQIRPVVVVTNLVFHSLGHLAELLAHHRLVAELRRCVLLLGLGSRE 607
EA V+ QP +S LGQ+RPVVVV +LV LGHLAELLAH LV E VLLL LG E
Sbjct: 459 EAAVNCTQPCESRLGQVRPVVVVADLVLDGLGHLAELLAHDSLVGEGSGFVLLLCLGRGE 280
Query: 608 DHSRVLCS 631
DHS +L S
Sbjct: 279 DHSHLLIS 256
Score = 84.9 bits (179), Expect(2) = 3e-059
Identities = 39/68 (57%), Positives = 47/68 (69%)
Frame = -3 / +3
Query: 830 SPAKGNGNRSRGSDLAGCEEEQLLPCEAVRQRQCQGAVHQGAEQPLQHPLLQTLGFGEQE 651
SP + NG+ S L C+EEQLLP +AV Q+Q QG+V QGA+QPLQ PLLQ L G+QE
Sbjct: 54 SPVRINGDGFGFSGLGACQEEQLLPDQAVWQQQRQGSVQQGAQQPLQCPLLQVLWPGKQE 233
Query: 650 DCDDPASC 627
D D A C
Sbjct: 234 DSDCAAGC 257
Score = 79.9 bits (168), Expect(2) = 1e-049
Identities = 41/70 (58%), Positives = 44/70 (62%)
Frame = -2 / +2
Query: 597 PRPRSRTHLRSSATSL*CARSSARWPXXXXXXXXXXXTGLI*PSQLLLG*ARCTSASRLP 418
PR RSRT+ S T L CARSSARWP TGL *PS L G* + T+ASRLP
Sbjct: 290 PRQRSRTNPLPSPTRLSCARSSARWPRPSRTRSATTTTGLT*PSLLSQG*VQFTAASRLP 469
Query: 417 SPVSRRRTGS 388
S V RRRTGS
Sbjct: 470 SLVLRRRTGS 499
Score = 63.4 bits (132), Expect(2) = 4e-046
Identities = 27/51 (52%), Positives = 31/51 (60%)
Frame = +1 / -2
Query: 679 RSGCCRGCSAPW*TAPWHCRCRTASQGRSCSSSQPARSDPLERLPFPFAGE 831
RSG CRGC AP *T PW C C+TA G SCSS Q R + + PF G+
Sbjct: 205 RSGHCRGCWAPC*TEPWRCCCQTA*SGSSCSS*QAPRPENPKPSPFILTGD 53
Score = 56.1 bits (116), Expect(2) = 5e-032
Identities = 23/25 (92%), Positives = 23/25 (92%)
Frame = +3 / -1
Query: 390 CLFFFLTPDLATWRLRYTELSLARA 464
CLFFFLTPDLATWRLR T LSLARA
Sbjct: 497 CLFFFLTPDLATWRLR*TALSLARA 423
Score = 30.9 bits (61), Expect(2) = 3e-021
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = +2 / -3
Query: 731 IAVAELLHKEGVVLPHNLPDQIPWNGCHFPSP 826
+AVA+LL +E VVL LPDQ N H P
Sbjct: 153 VAVAKLLDQEAVVLLDKLPDQRIRNRRHLS*P 58
>gb|CN816851.1|CN816851 HRO4525_E06_I11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4525_E06_I11, mRNA sequence
Length = 698
Score = 156 bits (336), Expect(2) = 6e-058
Identities = 68/81 (83%), Positives = 73/81 (90%)
Frame = -1 / +2
Query: 631 AAKDSAVVLSTTKTKKQNAPAKLSHKSVMRKEFRKMAKAVKNQVSDNYYRPDLTKPALAR 452
A K+ VVLSTTKTKKQN PA +HK+VMRKEFRKMAKAVKNQVSDNYYRPDLTKP LAR
Sbjct: 248 ADKEMTVVLSTTKTKKQNNPAAFTHKTVMRKEFRKMAKAVKNQVSDNYYRPDLTKPVLAR 427
Query: 451 LSSVYLSLQVAKSGVKKKNRQ 389
LS+V+ SLQVAKSGVKKKNRQ
Sbjct: 428 LSAVHRSLQVAKSGVKKKNRQ 490
Score = 137 bits (293), Expect(2) = 2e-045
Identities = 59/83 (71%), Positives = 62/83 (74%)
Frame = +1 / -1
Query: 382 LRTACSSS*HRTWQPGG*GTPSSA*QELAWSDQACSSCH*PGFSQPWPSCGTPCASQTCG 561
L TACSSS*H+TWQPGG G SA QE AWS QACSSC *PG QPWPSCGTPCA Q+CG
Sbjct: 497 LLTACSSS*HQTWQPGGCGELHSALQEQAWSSQACSSCR*PGS*QPWPSCGTPCA*QSCG 318
Query: 562 *ASQVRSASWSW**RGPQPSPLQ 630
* + SAS SW RGPQ SP Q
Sbjct: 317 *RQRGCSASLSWSWRGPQSSPCQ 249
Score = 132 bits (282), Expect(2) = 2e-050
Identities = 51/78 (65%), Positives = 64/78 (82%)
Frame = -1 / +2
Query: 814 MATVPGDLIWQVVRKNNSFLVKQFGNGNAKVQFTKEPNNLYNIHSFKHSGLANKKTVTIQ 635
MATV L+W++V+KNN FL+KQFGN NAKVQF+KEPNNLYN+HS+K+SGLANKKTVT+Q
Sbjct: 62 MATVSDSLVWELVKKNNCFLIKQFGNSNAKVQFSKEPNNLYNVHSYKYSGLANKKTVTVQ 241
Query: 634 PAAKDSAVVLSTTKTKKQ 581
PAA V+ +T K+
Sbjct: 242 PAADKEMTVVLSTTKTKK 295
Score = 96.8 bits (205), Expect(2) = 1e-030
Identities = 46/77 (59%), Positives = 53/77 (68%)
Frame = +3 / -3
Query: 585 FLVLVVERTTAESFAAGWIVTVFLFAKPECLKEWML*RLFGSLVNCTLALPLPNCFTRKE 764
F+++V T AAG VTVFLFA+PE L EW L*RL GSL+N TLAL LPNC RK+
Sbjct: 291 FVLVVERTTVISLSAAGCTVTVFLFARPEYL*EWTL*RLLGSLLN*TLALLLPNCLIRKQ 112
Query: 765 LFFLTTCQIRSPGTVAI 815
LFFLT+ Q TVAI
Sbjct: 111 LFFLTSSQTSESETVAI 61
Score = 88.6 bits (187), Expect(2) = 6e-022
Identities = 43/68 (63%), Positives = 48/68 (70%)
Frame = +2 / -2
Query: 428 EAEVHRAQPSKSWLGQIRPVVVVTNLVFHSLGHLAELLAHHRLVAELRRCVLLLGLGSRE 607
EA V+ QP K+ LGQ+RPVVVV +LV SLGHLAELLAH LV E VLLL LG E
Sbjct: 451 EAAVNCTQPCKNRLGQVRPVVVVADLVLDSLGHLAELLAHDSLVGEGSGVVLLLCLGRGE 272
Query: 608 DHSRVLCS 631
DHS +L S
Sbjct: 271 DHSHLLVS 248
Score = 83.1 bits (175), Expect(2) = 6e-058
Identities = 38/68 (55%), Positives = 47/68 (69%)
Frame = -3 / +1
Query: 830 SPAKGNGNRSRGSDLAGCEEEQLLPCEAVRQRQCQGAVHQGAEQPLQHPLLQTLGFGEQE 651
SP + NG+ + L C+EEQLLP +AV Q+Q QG+V QGA+QPLQ PLLQ L G+QE
Sbjct: 46 SPVRINGDGFGFTGLGACQEEQLLPDQAVWQQQRQGSVQQGAQQPLQCPLLQVLWPGKQE 225
Query: 650 DCDDPASC 627
D D A C
Sbjct: 226 DSDCAACC 249
Score = 82.6 bits (174), Expect(2) = 2e-050
Identities = 42/70 (60%), Positives = 44/70 (62%)
Frame = -2 / +3
Query: 597 PRPRSRTHLRSSATSL*CARSSARWPXXXXXXXXXXXTGLI*PSQLLLG*ARCTSASRLP 418
PR RSRT S T L CARSSARWP TGL *PS L G* + T+ASRLP
Sbjct: 282 PRQRSRTTPLPSPTRLSCARSSARWPRLSRTRSATTTTGLT*PSLFLQG*VQFTAASRLP 461
Query: 417 SPVSRRRTGS 388
S VSRRRTGS
Sbjct: 462 SLVSRRRTGS 491
Score = 67.5 bits (141), Expect(3) = 3e-021
Identities = 26/42 (61%), Positives = 32/42 (76%)
Frame = -3 / +1
Query: 626 KGLGCGPLYYQDQEAERTCEAQPQVCDAQGVPQDGQGCEKPG 501
+G CGPL+ QD+EAE+ PQ C AQGVPQDGQGC++PG
Sbjct: 253 QGDDCGPLHDQDKEAEQPRCLHPQDCHAQGVPQDGQGCQEPG 378
Score = 61.1 bits (127), Expect(2) = 2e-045
Identities = 27/51 (52%), Positives = 30/51 (58%)
Frame = +1 / -1
Query: 679 RSGCCRGCSAPW*TAPWHCRCRTASQGRSCSSSQPARSDPLERLPFPFAGE 831
RSG CRGC AP *T PW C C+TA G SCSS Q R + PF G+
Sbjct: 197 RSGHCRGCWAPC*TEPWRCCCQTA*SGSSCSS*QAPRPVNPKPSPFILTGD 45
Score = 54.2 bits (112), Expect(2) = 1e-030
Identities = 22/24 (91%), Positives = 22/24 (91%)
Frame = +3 / -3
Query: 390 CLFFFLTPDLATWRLRYTELSLAR 461
CLFFFLTPDLATWRLR T LSLAR
Sbjct: 489 CLFFFLTPDLATWRLR*TALSLAR 418
Score = 44.6 bits (91), Expect(3) = 3e-021
Identities = 17/21 (80%), Positives = 17/21 (80%)
Frame = -3 / +1
Query: 449 ELGVPQPPGCQVRCQEEEQAV 387
E PQPPGCQV CQEEEQAV
Sbjct: 430 ECSSPQPPGCQVWCQEEEQAV 492
Score = 30.4 bits (60), Expect(2) = 6e-022
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = +2 / -2
Query: 731 IAVAELLHKEGVVLPHNLPDQIPWNGCHFPSP 826
+AVA+LL +E VVL LPDQ N H P
Sbjct: 145 VAVAKLLDQEAVVLLDKLPDQ*IRNRRHLS*P 50
Score = 22.1 bits (42), Expect(3) = 3e-021
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2 / +3
Query: 828 SGEGKWQPFQGI*SGRL*GRTTPXXXXXSATA 733
+G KW+ F+ SG L RTT ATA
Sbjct: 48 TG*DKWRRFRIHWSGSLSRRTTAS*SSSLATA 143
>gb|CN816312.1|CN816312 HRO4519_C11_F21ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4519_C11_F21, mRNA sequence
Length = 509
Score = 165 bits (354), Expect = 1e-041
Identities = 71/81 (87%), Positives = 75/81 (92%)
Frame = -1 / +1
Query: 631 AAKDSAVVLSTTKTKKQNAPAKLSHKSVMRKEFRKMAKAVKNQVSDNYYRPDLTKPALAR 452
A D AVVLSTTKTKKQNAPAKL HK+VMRKEFRKMAKAVKNQV +NYYRPDLTKPALAR
Sbjct: 7 AGDDKAVVLSTTKTKKQNAPAKLQHKNVMRKEFRKMAKAVKNQVCNNYYRPDLTKPALAR 186
Query: 451 LSSVYLSLQVAKSGVKKKNRQ 389
LSSVY SLQV+KSG+KKKNRQ
Sbjct: 187 LSSVYRSLQVSKSGIKKKNRQ 249
Score = 122 bits (261), Expect = 9e-029
Identities = 54/79 (68%), Positives = 58/79 (73%)
Frame = +1 / -2
Query: 391 ACSSS*HRTWQPGG*GTPSSA*QELAWSDQACSSCH*PGFSQPWPSCGTPCASQTCG*AS 570
ACSSS* +TW+PGG G SSA QE AWSDQACSSC PG +PWPSCGTPCAS +C A
Sbjct: 247 ACSSS*CQTWKPGGCGRQSSALQEPAWSDQACSSCCKPGS*RPWPSCGTPCASHSCAGA* 68
Query: 571 QVRSASWSW**RGPQPSPL 627
Q RSASWS RG QP L
Sbjct: 67 QGRSASWSLSWRGRQPCHL 11
Score = 98.2 bits (208), Expect = 2e-021
Identities = 48/66 (72%), Positives = 49/66 (74%)
Frame = +2 / -3
Query: 428 EAEVHRAQPSKSWLGQIRPVVVVTNLVFHSLGHLAELLAHHRLVAELRRCVLLLGLGSRE 607
EA V RAQP KS LGQIRPVVVV NLV LGHLAELLAHH LV EL R VLLLGL E
Sbjct: 210 EAAVDRAQPCKSRLGQIRPVVVVANLVLDGLGHLAELLAHHILVLELSRGVLLLGLCRGE 31
Query: 608 DHSRVL 625
D S V+
Sbjct: 30 DDSLVI 13
Score = 75.3 bits (158), Expect(2) = 6e-025
Identities = 35/44 (79%), Positives = 35/44 (79%)
Frame = +3 / -1
Query: 501 TWFFTALAILRNSLRITDLWLSFAGAFCFLVLVVERTTAESFAA 632
TWF TALAILRNSLRIT L S AGAFCFLV VVERTTA S A
Sbjct: 137 TWFLTALAILRNSLRITFLCWSLAGAFCFLVFVVERTTALSSPA 6
Score = 53.8 bits (111), Expect(2) = 6e-025
Identities = 22/25 (88%), Positives = 22/25 (88%)
Frame = +3 / -1
Query: 390 CLFFFLTPDLATWRLRYTELSLARA 464
CLFFFL PDL TWRLR TELSLARA
Sbjct: 248 CLFFFLMPDLETWRLR*TELSLARA 174
>gb|CN819420.1|CN819420 HRO4405_G10_M19ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4405_G10_M19, mRNA
sequence
Length = 520
Score = 160 bits (345), Expect = 2e-040
Identities = 69/81 (85%), Positives = 74/81 (91%)
Frame = -1 / +3
Query: 631 AAKDSAVVLSTTKTKKQNAPAKLSHKSVMRKEFRKMAKAVKNQVSDNYYRPDLTKPALAR 452
A D AVVLSTTKTKKQNAP+KL HK+VMRKEFRKMAKAVKNQV +NYYRPDLT PALAR
Sbjct: 69 AGDDKAVVLSTTKTKKQNAPSKLQHKNVMRKEFRKMAKAVKNQVCNNYYRPDLTTPALAR 248
Query: 451 LSSVYLSLQVAKSGVKKKNRQ 389
LSSVY SLQV+KSG+KKKNRQ
Sbjct: 249 LSSVYRSLQVSKSGIKKKNRQ 311
Score = 129 bits (277), Expect = 5e-031
Identities = 55/79 (69%), Positives = 60/79 (75%)
Frame = +1 / -2
Query: 391 ACSSS*HRTWQPGG*GTPSSA*QELAWSDQACSSCH*PGFSQPWPSCGTPCASQTCG*AS 570
ACSSS* +TW+PGG GT SSA QE AWSDQACSSC PG QPWPSCGTPCAS +C A
Sbjct: 309 ACSSS*CQTWKPGGCGTQSSALQEPAWSDQACSSCCKPGS*QPWPSCGTPCASHSCAGA* 130
Query: 571 QVRSASWSW**RGPQPSPL 627
+ RSASWSW RG +P L
Sbjct: 129 KGRSASWSWSWRGQRPCHL 73
Score = 107 bits (228), Expect = 3e-024
Identities = 50/66 (75%), Positives = 52/66 (78%)
Frame = +2 / -3
Query: 428 EAEVHRAQPSKSWLGQIRPVVVVTNLVFHSLGHLAELLAHHRLVAELRRCVLLLGLGSRE 607
EA VHRAQP KS GQIRPVVVV NLV SLGHLAELLAHH LV ELRR VLLLGLG E
Sbjct: 272 EAAVHRAQPCKSRRGQIRPVVVVANLVLDSLGHLAELLAHHILVLELRRGVLLLGLGRGE 93
Query: 608 DHSRVL 625
D+ V+
Sbjct: 92 DNGLVI 75
Score = 98.7 bits (209), Expect(2) = 2e-027
Identities = 46/70 (65%), Positives = 50/70 (71%)
Frame = -2 / +1
Query: 597 PRPRSRTHLRSSATSL*CARSSARWPXXXXXXXXXXXTGLI*PSQLLLG*ARCTSASRLP 418
PRPRSRT L SS+T +*CARSSARWP TGLI*P +LL G*A CT+ASR P
Sbjct: 103 PRPRSRTPLLSSSTRM*CARSSARWPRLSRTRFATTTTGLI*PRRLLQG*ALCTAASRFP 282
Query: 417 SPVSRRRTGS 388
S SRRRTGS
Sbjct: 283 SLASRRRTGS 312
Score = 74.8 bits (157), Expect(2) = 3e-026
Identities = 35/44 (79%), Positives = 35/44 (79%)
Frame = +3 / -1
Query: 501 TWFFTALAILRNSLRITDLWLSFAGAFCFLVLVVERTTAESFAA 632
TWF TALAILRNSLRIT L S GAFCFLVLVVERTTA S A
Sbjct: 199 TWFLTALAILRNSLRITFLCWSLEGAFCFLVLVVERTTALSSPA 68
Score = 58.8 bits (122), Expect(2) = 3e-026
Identities = 23/25 (92%), Positives = 23/25 (92%)
Frame = +3 / -1
Query: 390 CLFFFLTPDLATWRLRYTELSLARA 464
CLFFFL PDL TWRLRYTELSLARA
Sbjct: 310 CLFFFLMPDLETWRLRYTELSLARA 236
Score = 39.1 bits (79), Expect(2) = 2e-027
Identities = 17/30 (56%), Positives = 21/30 (70%)
Frame = -1 / +3
Query: 670 SGLANKKTVTIQPAAKDSAVVLSTTKTKKQ 581
SGLAN KTVTIQP+A D V+ +T K+
Sbjct: 27 SGLANSKTVTIQPSAGDDKAVVLSTTKTKK 116
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,414,953
Number of Sequences: 8143
Number of extensions: 182205
Number of successful extensions: 1248
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 318942556
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)