TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.65
(673 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Aven... 221 1e-058
gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Aven... 215 4e-057
gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Aven... 210 2e-055
gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Aven... 210 3e-055
gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Aven... 206 2e-054
gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Aven... 204 9e-054
gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Aven... 159 5e-040
gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Aven... 117 2e-027
gb|CN816143.1|CN816143 HRO4517_E01_I01ZS5 Lib AA071E1X Aven... 94 2e-024
>gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4507_C01_F01, mRNA sequence
Length = 365
Score = 221 bits (476), Expect = 1e-058
Identities = 99/113 (87%), Positives = 101/113 (89%)
Frame = +3 / +3
Query: 201 GKSAGKEGGVDXXXXXXXXXSVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEK 380
GK+A KEGG SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEK
Sbjct: 6 GKTASKEGGEGKKGKKKAKKSVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEK 185
Query: 381 LAGEAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 539
LAGE+AKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 186 LAGESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 344
Score = 191 bits (411), Expect = 1e-049
Identities = 86/113 (76%), Positives = 89/113 (78%)
Frame = -1 / -2
Query: 538 *DEVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSLM 359
*++VN VTALVPSETACLASSPGRTRRTEVWISREVMVGF SKMSLM
Sbjct: 343 *EDVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFLL*RASLADSPASFSKMSLM 164
Query: 358 KEFMMDMALEEMPMSGCTCFSTLKM*ILYVSTLFLAFFRPFLSTPPSLPADFP 200
KEFMMDMALEEMPMSGCTC TLKM*IL VSTLF AFF PF +PPSL A FP
Sbjct: 163 KEFMMDMALEEMPMSGCTCLRTLKM*IL*VSTLFFAFFLPFFPSPPSLEAVFP 5
>gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_A08_B15, mRNA sequence
Length = 655
Score = 215 bits (465), Expect = 4e-057
Identities = 97/111 (87%), Positives = 99/111 (89%)
Frame = +3 / +3
Query: 210 AGKEGGVDXXXXXXXXXSVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAG 389
A KEGG SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAG
Sbjct: 27 ASKEGGEGKKGKKKAKKSVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAG 206
Query: 390 EAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*A 542
E+AKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*+
Sbjct: 207 ESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*S 359
Score = 199 bits (429), Expect = 4e-052
Identities = 88/110 (80%), Positives = 90/110 (81%)
Frame = -1 / -1
Query: 538 *DEVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSLM 359
*DEVN VTALVPS+TACLASSPGRTRRTEVWISREVMVGF SKMSLM
Sbjct: 355 *DEVNFVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLLYLASFADSPASFSKMSLM 176
Query: 358 KEFMMDMALEEMPMSGCTCFSTLKM*ILYVSTLFLAFFRPFLSTPPSLPA 209
KEFMMDMALEEMPMSGCTCF TLKM*ILYVSTLFLAFF PF +PPSL A
Sbjct: 175 KEFMMDMALEEMPMSGCTCFRTLKM*ILYVSTLFLAFFLPFFPSPPSLDA 26
>gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4408_D06_H12, mRNA
sequence
Length = 415
Score = 210 bits (453), Expect = 2e-055
Identities = 93/94 (98%), Positives = 94/94 (100%)
Frame = +3 / +2
Query: 261 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSR 440
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSR
Sbjct: 38 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSR 217
Query: 441 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*A 542
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*A
Sbjct: 218 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*A 319
Score = 171 bits (368), Expect = 1e-043
Identities = 80/106 (75%), Positives = 83/106 (78%)
Frame = -1 / -2
Query: 541 A*DEVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSL 362
A*++VNLVTALVPSETACLASSPGRTRRTEV ISREVMVGF SKMSL
Sbjct: 318 A*EDVNLVTALVPSETACLASSPGRTRRTEVCISREVMVGFLL*RASLADSPASFSKMSL 139
Query: 361 MKEFMMDMALEEMPMSGCTCFSTLKM*ILYVSTLFLAFFRPFLSTP 224
MKEFMMDMALE+MPMSGCTC STLKM*IL VSTLF AFF P P
Sbjct: 138 MKEFMMDMALEDMPMSGCTCLSTLKM*IL*VSTLFFAFFLPSSLVP 1
>gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4527_G09_N17, mRNA sequence
Length = 412
Score = 210 bits (452), Expect = 3e-055
Identities = 93/93 (100%), Positives = 93/93 (100%)
Frame = +3 / +1
Query: 261 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSR 440
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSR
Sbjct: 25 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSR 204
Query: 441 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 539
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 205 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 303
Score = 176 bits (378), Expect = 4e-045
Identities = 79/96 (82%), Positives = 80/96 (83%)
Frame = -1 / -3
Query: 535 DEVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSLMK 356
DEVNLVTALVPS+TACLASSPGRTRRTEVWISREVMVGF SKMSLMK
Sbjct: 299 DEVNLVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLL*RASLAASPASFSKMSLMK 120
Query: 355 EFMMDMALEEMPMSGCTCFSTLKM*ILYVSTLFLAF 248
EFMMDMALEEMPMSGCTCFSTLKM*IL VSTLF AF
Sbjct: 119 EFMMDMALEEMPMSGCTCFSTLKM*IL*VSTLFFAF 12
>gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_B07_C14, mRNA
sequence
Length = 454
Score = 206 bits (445), Expect = 2e-054
Identities = 91/92 (98%), Positives = 92/92 (100%)
Frame = +3 / +1
Query: 264 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 443
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSRE
Sbjct: 13 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 192
Query: 444 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 539
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 193 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 288
Score = 158 bits (339), Expect = 1e-039
Identities = 72/92 (78%), Positives = 74/92 (80%)
Frame = -1 / -3
Query: 538 *DEVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSLM 359
*++VN VTALVPSETACLASSPGRTRRTEVWISREVMVGF SKMSLM
Sbjct: 287 *EDVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFLL*RASLADSPASFSKMSLM 108
Query: 358 KEFMMDMALEEMPMSGCTCFSTLKM*ILYVST 263
KEFMMDMALEEMPMSGCTC TLKM*IL VST
Sbjct: 107 KEFMMDMALEEMPMSGCTCLRTLKM*IL*VST 12
>gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4516_H02_P04, mRNA sequence
Length = 472
Score = 204 bits (441), Expect = 9e-054
Identities = 90/91 (98%), Positives = 91/91 (100%)
Frame = +3 / +2
Query: 267 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSREI 446
ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSREI
Sbjct: 11 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREI 190
Query: 447 QTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 539
QTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 191 QTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 283
Score = 148 bits (317), Expect = 1e-036
Identities = 69/92 (75%), Positives = 72/92 (78%)
Frame = -1 / -2
Query: 541 A*DEVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSL 362
+*+EVNLVTALVPSETACLASSPG TRRTEVWIS EVMVGF SK+SL
Sbjct: 285 S*EEVNLVTALVPSETACLASSPGSTRRTEVWISLEVMVGFLLYLASLADSPASFSKISL 106
Query: 361 MKEFMMDMALEEMPMSGCTCFSTLKM*ILYVS 266
MKEFMMDMALEEMPMSG TC TLKM*IL VS
Sbjct: 105 MKEFMMDMALEEMPMSGWTCLRTLKM*IL*VS 10
>gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_C10_F20, mRNA sequence
Length = 360
Score = 159 bits (341), Expect = 5e-040
Identities = 72/74 (97%), Positives = 73/74 (98%)
Frame = +3 / +2
Query: 321 GISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS 500
GISS AMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS
Sbjct: 11 GISS*AMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS 190
Query: 501 EGTKAVTKFTSS*A 542
EGTKAVTKFTSS*A
Sbjct: 191 EGTKAVTKFTSS*A 232
Score = 117 bits (251), Expect(2) = 2e-028
Identities = 55/75 (73%), Positives = 59/75 (78%)
Frame = -1 / -1
Query: 541 A*DEVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSL 362
A*++VNLVTALVPSETACLASSPGRTRRTEV ISREVMVGF SKMSL
Sbjct: 231 A*EDVNLVTALVPSETACLASSPGRTRRTEVCISREVMVGFLL*RASLADSPASFSKMSL 52
Query: 361 MKEFMMDMALEEMPM 317
MKEFMMDMA E+MP+
Sbjct: 51 MKEFMMDMA*EDMPL 7
Score = 22.1 bits (42), Expect(2) = 2e-028
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = -3 / -2
Query: 671 FFFFFFARIV 642
FFFFFF I+
Sbjct: 359 FFFFFFGSII 330
>gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_F01_K01, mRNA sequence
Length = 361
Score = 117 bits (250), Expect = 2e-027
Identities = 53/55 (96%), Positives = 54/55 (98%)
Frame = +3 / +3
Query: 375 EKLAGEAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 539
EKLA E+AKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 12 EKLACESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 176
>gb|CN816143.1|CN816143 HRO4517_E01_I01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_E01_I01, mRNA sequence
Length = 384
Score = 93.6 bits (198), Expect(2) = 2e-024
Identities = 42/44 (95%), Positives = 42/44 (95%)
Frame = +3 / +2
Query: 417 KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*APF 548
KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* F
Sbjct: 56 KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*VRF 187
Score = 33.6 bits (67), Expect(2) = 2e-024
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = +3 / +3
Query: 372 FEKLAGEAAKLARYNKKP 425
FEKL+GE+AKLAR+ + P
Sbjct: 12 FEKLSGESAKLARFTRSP 65
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,505,038
Number of Sequences: 8143
Number of extensions: 54177
Number of successful extensions: 3672
Number of sequences better than 1.0e-020: 9
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 221812061
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)