TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.645
(1154 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815339.1|CN815339 HRO4508_F01_L02ZS5 Lib AA071E1X Aven... 275 2e-083
gb|AF117889.1|AF117889 Avena fatua 40S ribosomal protein S3... 154 3e-038
gb|CN821129.1|CN821129 HRO4427_G12_N23ZS5 Lib AA069E1X Aven... 127 3e-030
gb|CN816113.1|CN816113 HRO4517_B05_C09ZS5 Lib AA071E1X Aven... 118 2e-027
>gb|CN815339.1|CN815339 HRO4508_F01_L02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_F01_L02, mRNA sequence
Length = 525
Score = 275 bits (596), Expect(2) = 2e-083
Identities = 112/124 (90%), Positives = 121/124 (97%)
Frame = -3 / +1
Query: 711 KKWQTLIEAHVDVKTTDNYMLRLFCIGFTKRRPNQVKRTCYAQASQIRQIRRKMVEIMIN 532
KKWQTLIEAHVDVKTTDNYMLRLFCIGFTKRRPNQVKRTCYAQASQIRQIRRKMVEIM N
Sbjct: 25 KKWQTLIEAHVDVKTTDNYMLRLFCIGFTKRRPNQVKRTCYAQASQIRQIRRKMVEIMAN 204
Query: 531 QASTCDLKELVSKFIPEVIGKEIEKSTSSIFPLQNVFIRKVKILKAPKFDLGKLMEVHGD 352
ASTCDLKELV+KFIPEVIGK+IEK+TSSIFPLQNV++RKVKILK+PKFD+GKLMEVHGD
Sbjct: 205 HASTCDLKELVAKFIPEVIGKDIEKATSSIFPLQNVYVRKVKILKSPKFDIGKLMEVHGD 384
Query: 351 YKED 340
Y ++
Sbjct: 385 YNKE 396
Score = 197 bits (425), Expect(2) = 2e-055
Identities = 88/114 (77%), Positives = 93/114 (81%)
Frame = +2 / -2
Query: 368 INFPRSNLGALSIFTLRMKTF*SGKMLEVDFSISLPITSGMNLETSSLRSHVEAWLIIIS 547
INFP SN G IFTLR TF SGKMLEV FSIS P+TSG+N TSS +S VEAWL +IS
Sbjct: 368 INFPISNFGDFRIFTLRTYTFWSGKMLEVAFSISFPMTSGINFATSSFKSQVEAWLAMIS 189
Query: 548 TILRRICRIWDA*A*QVLLTWFGRLLVKPMQNKRSM*LSVVLTSTWASINVCHF 709
TI RRIC IW A*A*QV TWFGRLLVKPMQN RSM*LSVVLTSTWASI+VCHF
Sbjct: 188 TIFRRICLIWLA*A*QVRFTWFGRLLVKPMQNSRSM*LSVVLTSTWASISVCHF 27
Score = 90.0 bits (190), Expect(2) = 1e-026
Identities = 38/65 (58%), Positives = 45/65 (69%)
Frame = -2 / +3
Query: 709 EVADID*SPC*CQDNRQLHAAFVLHWFYQEAPKPGQENLLCSGIPDSADSPQDG*DYDQP 530
+VAD +* PC*C D QLHA VLHW + E KPG+ LLCSG PD DSP+DG D+ QP
Sbjct: 27 KVADTN*GPC*C*DY*QLHAPAVLHWLH*ETSKPGEAYLLCSGQPDQTDSPEDGRDHGQP 206
Query: 529 GFYM* 515
F +*
Sbjct: 207 RFNL* 221
Score = 83.1 bits (175), Expect(2) = 4e-024
Identities = 38/64 (59%), Positives = 46/64 (71%)
Frame = +3 / -3
Query: 516 HM*KPG*S*SQPSCGESAESGMPEHSRFS*PGLGASW*NQCRTNAACSCRLS*HQHGLQS 695
H K G *S+PS GES SG PEHS+++ PGL S *+QCRT AC+C+ S*HQHG Q
Sbjct: 220 HRLKRGWP*SRPSSGESV*SGWPEHSKYASPGLDVS**SQCRTAGACNCQ*S*HQHGPQL 41
Query: 696 MSAT 707
+SAT
Sbjct: 40 VSAT 29
Score = 49.2 bits (101), Expect(2) = 2e-083
Identities = 21/33 (63%), Positives = 23/33 (69%)
Frame = -3 / +1
Query: 348 KEDVGVKLERPVEGDEAMQEVAAAE*FICYFFI 250
KED+GVKL+RP EGDEAM AE* C F I
Sbjct: 391 KEDIGVKLDRPAEGDEAMATEVTAE*SWCCFLI 489
Score = 45.1 bits (92), Expect(2) = 1e-026
Identities = 21/46 (45%), Positives = 30/46 (65%)
Frame = -2 / +3
Query: 478 YWQRD*EVYLQHLPTSKRLHPQGEDTQGPQV*SREVDGGSWRLQRG 341
+W+R *E +H PT K + PQG+D + +V EVDGG+ LQ+G
Sbjct: 258 HWKRY*EGNFKHFPTPKCVCPQGKDPEVTKVRYWEVDGGTR*LQQG 395
Score = 43.7 bits (89), Expect(2) = 4e-024
Identities = 22/46 (47%), Positives = 26/46 (56%)
Frame = +3 / -3
Query: 342 PLCSRHEPPSTSRDQTWGP*VSSPCG*RRFEVGRCWR*TSQSLCQ* 479
P CS PPSTS+ +T S PCG F VG+C + SQ L Q*
Sbjct: 394 PCCSHRVPPSTSQYRTLVTSGSLPCGHTHFGVGKCLKLPSQYLFQ* 257
Score = 34.5 bits (69), Expect(2) = 2e-055
Identities = 15/36 (41%), Positives = 22/36 (61%)
Frame = +1 / -1
Query: 247 LDKEVANKLLGSSNLLHSLITLDRPLKLHTNVLFVV 354
L+ E LL + H LI+ RP++LHT++L VV
Sbjct: 492 LN*ETTPTLLSCNLRCHRLISFSRPIQLHTDILLVV 385
>gb|AF117889.1|AF117889 Avena fatua 40S ribosomal protein S3A mRNA, partial cds
Length = 382
Score = 154 bits (330), Expect = 3e-038
Identities = 62/74 (83%), Positives = 69/74 (93%)
Frame = -3 / +2
Query: 516 DLKELVSKFIPEVIGKEIEKSTSSIFPLQNVFIRKVKILKAPKFDLGKLMEVHGDYKEDV 337
DLKELV KFIPE+IGKEIEK+T SIFPLQNV++RKVKILKAPKFDLGKLMEVHGDYKEDV
Sbjct: 2 DLKELVQKFIPELIGKEIEKATPSIFPLQNVYVRKVKILKAPKFDLGKLMEVHGDYKEDV 181
Query: 336 GVKLERPVEGDEAM 295
G KL+RP +GDE +
Sbjct: 182 GTKLDRPADGDEVI 223
>gb|CN821129.1|CN821129 HRO4427_G12_N23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_G12_N23, mRNA
sequence
Length = 497
Score = 127 bits (272), Expect = 3e-030
Identities = 50/60 (83%), Positives = 57/60 (95%)
Frame = -3 / +3
Query: 474 GKEIEKSTSSIFPLQNVFIRKVKILKAPKFDLGKLMEVHGDYKEDVGVKLERPVEGDEAM 295
GKEIEK+T+SIFPLQNV++RKVKILKAPKFDLGKLMEVHGDYKEDVG KL+RP +GDE +
Sbjct: 12 GKEIEKATASIFPLQNVYVRKVKILKAPKFDLGKLMEVHGDYKEDVGTKLDRPADGDEVI 191
>gb|CN816113.1|CN816113 HRO4517_B05_C09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_B05_C09, mRNA sequence
Length = 846
Score = 118 bits (252), Expect = 2e-027
Identities = 46/56 (82%), Positives = 53/56 (94%)
Frame = -3 / +2
Query: 462 EKSTSSIFPLQNVFIRKVKILKAPKFDLGKLMEVHGDYKEDVGVKLERPVEGDEAM 295
EK+T+SIFPLQNV++RKVKILKAPKFDLGKLMEVHGDYKEDVG KL+RP +GDE +
Sbjct: 539 EKATASIFPLQNVYVRKVKILKAPKFDLGKLMEVHGDYKEDVGTKLDRPADGDEVI 706
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,540,396
Number of Sequences: 8143
Number of extensions: 181831
Number of successful extensions: 1270
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 411126300
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)