TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.49
(1833 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Aven... 184 3e-047
gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Aven... 182 1e-046
gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Aven... 181 4e-046
gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Aven... 180 6e-046
gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Aven... 180 6e-046
gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Aven... 178 2e-045
gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Aven... 135 2e-032
gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Aven... 100 8e-022
>gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4408_D06_H12, mRNA
sequence
Length = 415
Score = 184 bits (397), Expect = 3e-047
Identities = 83/98 (84%), Positives = 84/98 (85%)
Frame = +3 / +2
Query: 1191 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSRE 1370
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSRE
Sbjct: 41 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 220
Query: 1371 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSA*AARPPL 1484
IQTSVRLVLPGELAKHAVSEGTKAVTKFTS+*AA L
Sbjct: 221 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*AAAQQL 334
Score = 148 bits (317), Expect = 3e-036
Identities = 70/94 (74%), Positives = 73/94 (77%)
Frame = -3 / -2
Query: 1471 AA*AEVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXXXXX 1292
AA* +VNLVTALVPSETACLASSPGRTRRTEV ISREVMVGF
Sbjct: 321 AA*EDVNLVTALVPSETACLASSPGRTRRTEVCISREVMVGFLL*RASLADSPASFSKMS 142
Query: 1291 LMKEFMIDMALEEMPMSGCTCFSTLKM*IL*VST 1190
LMKEFM+DMALE+MPMSGCTC STLKM*IL*VST
Sbjct: 141 LMKEFMMDMALEDMPMSGCTCLSTLKM*IL*VST 40
Score = 87.2 bits (184), Expect(2) = 2e-026
Identities = 39/53 (73%), Positives = 44/53 (83%)
Frame = +2 / +1
Query: 1196 DLQDLHLQGAEAGAPGHWHLLQGHVDHELLH*RYLREARC*GRQARAL*QEAY 1354
DLQDLHLQGA+AGAPGH H+LQGHV HELLH*R+L EA QAR+L QEA+
Sbjct: 46 DLQDLHLQGAQAGAPGHRHILQGHVHHELLH*RHL*EACWGVCQARSLQQEAH 204
Score = 48.3 bits (99), Expect(2) = 2e-026
Identities = 21/31 (67%), Positives = 23/31 (74%)
Frame = +2 / +1
Query: 1400 RGARQARRLRGHQGRHQVHLGLGCAAAACRI 1492
R ARQAR L GHQG HQVH+ LGC AA R+
Sbjct: 250 RRARQARCL*GHQGCHQVHILLGCCAATGRL 342
>gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4527_G09_N17, mRNA sequence
Length = 412
Score = 182 bits (393), Expect = 1e-046
Identities = 81/95 (85%), Positives = 83/95 (87%)
Frame = +3 / +1
Query: 1191 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSRE 1370
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSRE
Sbjct: 28 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 207
Query: 1371 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSA*AAR 1475
IQTSVRLVLPGELAKHAVSEGTKAVTKFTS+* +R
Sbjct: 208 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*RSR 312
Score = 155 bits (332), Expect = 3e-038
Identities = 70/90 (77%), Positives = 72/90 (80%)
Frame = -3 / -3
Query: 1459 EVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXXXXXLMKE 1280
EVNLVTALVPS+TACLASSPGRTRRTEVWISREVMVGF LMKE
Sbjct: 296 EVNLVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLL*RASLAASPASFSKMSLMKE 117
Query: 1279 FMIDMALEEMPMSGCTCFSTLKM*IL*VST 1190
FM+DMALEEMPMSGCTCFSTLKM*IL*VST
Sbjct: 116 FMMDMALEEMPMSGCTCFSTLKM*IL*VST 27
Score = 95.0 bits (201), Expect(2) = 2e-028
Identities = 42/53 (79%), Positives = 44/53 (83%)
Frame = +2 / +3
Query: 1196 DLQDLHLQGAEAGAPGHWHLLQGHVDHELLH*RYLREARC*GRQARAL*QEAY 1354
DLQDLHLQGAEAGAPGH HLLQGHV HELLH R+L E R GRQA AL QEA+
Sbjct: 33 DLQDLHLQGAEAGAPGHRHLLQGHVHHELLHQRHL*ETRWRGRQAGALQQEAH 191
Score = 46.9 bits (96), Expect(2) = 2e-028
Identities = 18/20 (90%), Positives = 19/20 (95%)
Frame = +2 / +3
Query: 1400 RGARQARRLRGHQGRHQVHL 1459
R ARQARR+RGHQGRHQVHL
Sbjct: 237 RRARQARRVRGHQGRHQVHL 296
>gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_A08_B15, mRNA sequence
Length = 655
Score = 181 bits (389), Expect = 4e-046
Identities = 80/93 (86%), Positives = 82/93 (88%)
Frame = +3 / +3
Query: 1191 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSRE 1370
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSRE
Sbjct: 81 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 260
Query: 1371 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSA*A 1469
IQTSVRLVLPGELAKHAVSEGTKAVTKFTS+*+
Sbjct: 261 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*S 359
Score = 149 bits (320), Expect = 1e-036
Identities = 67/90 (74%), Positives = 69/90 (76%)
Frame = -3 / -1
Query: 1459 EVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXXXXXLMKE 1280
EVN VTALVPS+TACLASSPGRTRRTEVWISREVMVGF LMKE
Sbjct: 349 EVNFVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLLYLASFADSPASFSKMSLMKE 170
Query: 1279 FMIDMALEEMPMSGCTCFSTLKM*IL*VST 1190
FM+DMALEEMPMSGCTCF TLKM*IL VST
Sbjct: 169 FMMDMALEEMPMSGCTCFRTLKM*ILYVST 80
Score = 75.3 bits (158), Expect(2) = 4e-022
Identities = 34/53 (64%), Positives = 39/53 (73%)
Frame = +2 / +2
Query: 1196 DLQDLHLQGAEAGAPGHWHLLQGHVDHELLH*RYLREARC*GRQARAL*QEAY 1354
D+QDLHLQG EAGA H HLLQGHV HELLH R+LRE R +A + QEA+
Sbjct: 86 DIQDLHLQGPEAGASRHRHLLQGHVHHELLHQRHLRETRWRVGKACEVQQEAH 244
Score = 45.5 bits (93), Expect(2) = 4e-022
Identities = 18/25 (72%), Positives = 21/25 (84%)
Frame = +2 / +2
Query: 1400 RGARQARRLRGHQGRHQVHLGLGCA 1474
RGARQA R+RGHQG H+VHL L C+
Sbjct: 290 RGARQACRVRGHQGCHEVHLVLVCS 364
>gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4507_C01_F01, mRNA sequence
Length = 365
Score = 180 bits (388), Expect = 6e-046
Identities = 80/92 (86%), Positives = 81/92 (88%)
Frame = +3 / +3
Query: 1191 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSRE 1370
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSRE
Sbjct: 69 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 248
Query: 1371 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSA* 1466
IQTSVRLVLPGELAKHAVSEGTKAVTKFTS+*
Sbjct: 249 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 344
Score = 147 bits (316), Expect = 5e-036
Identities = 67/90 (74%), Positives = 69/90 (76%)
Frame = -3 / -2
Query: 1459 EVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXXXXXLMKE 1280
+VN VTALVPSETACLASSPGRTRRTEVWISREVMVGF LMKE
Sbjct: 337 DVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFLL*RASLADSPASFSKMSLMKE 158
Query: 1279 FMIDMALEEMPMSGCTCFSTLKM*IL*VST 1190
FM+DMALEEMPMSGCTC TLKM*IL*VST
Sbjct: 157 FMMDMALEEMPMSGCTCLRTLKM*IL*VST 68
Score = 86.8 bits (183), Expect(2) = 2e-023
Identities = 39/53 (73%), Positives = 42/53 (79%)
Frame = +2 / +2
Query: 1196 DLQDLHLQGAEAGAPGHWHLLQGHVDHELLH*RYLREARC*GRQARAL*QEAY 1354
DLQDLHLQG +AGAPGH HLLQGHV HELLH R+L E R QAR L*QEA+
Sbjct: 74 DLQDLHLQGPQAGAPGHRHLLQGHVHHELLHQRHL*ETRWRVCQARPL*QEAH 232
Score = 38.6 bits (78), Expect(2) = 2e-023
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +2 / +2
Query: 1400 RGARQARRLRGHQGRHQVHLGLGCAAAAC 1486
R A QAR LRGHQG H+V++ LG + C
Sbjct: 278 RRACQARGLRGHQGCHKVYILLGSGSLGC 364
>gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_B07_C14, mRNA
sequence
Length = 454
Score = 180 bits (388), Expect = 6e-046
Identities = 80/92 (86%), Positives = 81/92 (88%)
Frame = +3 / +1
Query: 1191 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSRE 1370
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSRE
Sbjct: 13 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 192
Query: 1371 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSA* 1466
IQTSVRLVLPGELAKHAVSEGTKAVTKFTS+*
Sbjct: 193 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 288
Score = 147 bits (316), Expect = 5e-036
Identities = 67/90 (74%), Positives = 69/90 (76%)
Frame = -3 / -3
Query: 1459 EVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXXXXXLMKE 1280
+VN VTALVPSETACLASSPGRTRRTEVWISREVMVGF LMKE
Sbjct: 281 DVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFLL*RASLADSPASFSKMSLMKE 102
Query: 1279 FMIDMALEEMPMSGCTCFSTLKM*IL*VST 1190
FM+DMALEEMPMSGCTC TLKM*IL*VST
Sbjct: 101 FMMDMALEEMPMSGCTCLRTLKM*IL*VST 12
Score = 90.9 bits (192), Expect(2) = 1e-027
Identities = 40/53 (75%), Positives = 43/53 (81%)
Frame = +2 / +3
Query: 1196 DLQDLHLQGAEAGAPGHWHLLQGHVDHELLH*RYLREARC*GRQARAL*QEAY 1354
DLQDLHLQG +AGAPGH HLLQGHV HELLH R+LREAR RQA L QEA+
Sbjct: 18 DLQDLHLQGPQAGAPGHRHLLQGHVHHELLHQRHLREARWRVRQAGPLQQEAH 176
Score = 48.3 bits (99), Expect(2) = 1e-027
Identities = 19/31 (61%), Positives = 23/31 (74%)
Frame = +2 / +3
Query: 1400 RGARQARRLRGHQGRHQVHLGLGCAAAACRI 1492
RGARQAR L GHQGRH+VH+ LG + C +
Sbjct: 222 RGARQARCL*GHQGRHKVHILLGSGSLGCSL 314
>gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4516_H02_P04, mRNA sequence
Length = 472
Score = 178 bits (384), Expect = 2e-045
Identities = 79/91 (86%), Positives = 80/91 (87%)
Frame = +3 / +2
Query: 1194 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSREI 1373
ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF RYNKKPTITSREI
Sbjct: 11 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREI 190
Query: 1374 QTSVRLVLPGELAKHAVSEGTKAVTKFTSA* 1466
QTSVRLVLPGELAKHAVSEGTKAVTKFTS+*
Sbjct: 191 QTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 283
Score = 141 bits (303), Expect = 3e-034
Identities = 67/94 (71%), Positives = 69/94 (73%)
Frame = -3 / -2
Query: 1474 RAA*AEVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXXXX 1295
R +* EVNLVTALVPSETACLASSPG TRRTEVWIS EVMVGF
Sbjct: 291 RTS*EEVNLVTALVPSETACLASSPGSTRRTEVWISLEVMVGFLLYLASLADSPASFSKI 112
Query: 1294 XLMKEFMIDMALEEMPMSGCTCFSTLKM*IL*VS 1193
LMKEFM+DMALEEMPMSG TC TLKM*IL*VS
Sbjct: 111 SLMKEFMMDMALEEMPMSGWTCLRTLKM*IL*VS 10
Score = 123 bits (264), Expect = 7e-029
Identities = 58/93 (62%), Positives = 62/93 (66%)
Frame = +2 / +1
Query: 1196 DLQDLHLQGAEAGAPGHWHLLQGHVDHELLH*RYLREARC*GRQARAL*QEAYXXXXXXX 1375
DLQDLHLQG +AG P H HLLQGHV HELLH RYL EA QAR + QEA+
Sbjct: 13 DLQDLHLQGPQAGPPRHRHLLQGHVHHELLHQRYL*EAGWRVCQAREVQQEAHHHLQGDP 192
Query: 1376 XXXXXXXSRGARQARRLRGHQGRHQVHLGLGCA 1474
SRGARQARRL GHQGRHQVH LGC+
Sbjct: 193 DFCSSCASRGARQARRL*GHQGRHQVHFLLGCS 291
>gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_C10_F20, mRNA sequence
Length = 360
Score = 135 bits (289), Expect = 2e-032
Identities = 63/79 (79%), Positives = 64/79 (81%)
Frame = +3 / +2
Query: 1248 GISSKAMSIMNSFINDIFXXXXXXXXXXXRYNKKPTITSREIQTSVRLVLPGELAKHAVS 1427
GISS AMSIMNSFINDIF RYNKKPTITSREIQTSVRLVLPGELAKHAVS
Sbjct: 11 GISS*AMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS 190
Query: 1428 EGTKAVTKFTSA*AARPPL 1484
EGTKAVTKFTS+*AA L
Sbjct: 191 EGTKAVTKFTSS*AAAQQL 247
Score = 106 bits (227), Expect = 9e-024
Identities = 51/76 (67%), Positives = 55/76 (72%)
Frame = -3 / -1
Query: 1471 AA*AEVNLVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXXXXX 1292
AA* +VNLVTALVPSETACLASSPGRTRRTEV ISREVMVGF
Sbjct: 234 AA*EDVNLVTALVPSETACLASSPGRTRRTEVCISREVMVGFLL*RASLADSPASFSKMS 55
Query: 1291 LMKEFMIDMALEEMPM 1244
LMKEFM+DMA E+MP+
Sbjct: 54 LMKEFMMDMA*EDMPL 7
>gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_F01_K01, mRNA sequence
Length = 361
Score = 100 bits (213), Expect = 8e-022
Identities = 44/46 (95%), Positives = 45/46 (97%)
Frame = +3 / +3
Query: 1335 RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSA*AA 1472
RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTS+* A
Sbjct: 45 RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*TA 182
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,345,479
Number of Sequences: 8143
Number of extensions: 160081
Number of successful extensions: 1364
Number of sequences better than 1.0e-020: 8
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 678594380
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)