TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.489
(1666 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815233.1|CN815233 HRO4507_D04_H07ZS5 Lib AA071E1X Aven... 395 e-111
gb|AI978372.1|AI978372 CDO507.R Oat Etiolated Leaf cDNA Lib... 107 4e-024
>gb|CN815233.1|CN815233 HRO4507_D04_H07ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4507_D04_H07, mRNA sequence
Length = 654
Score = 395 bits (857), Expect(2) = e-111
Identities = 167/183 (91%), Positives = 174/183 (95%)
Frame = +2 / +3
Query: 803 LANDAKAAKGGGERQFNGLVDVYRKTLKSDGIAGLYRGFNISCVGIIVYRGLYFGLYDSI 982
LANDAKA+KGGGERQFNGLVDVYRKTLK+DGIAGLYRGFNISCVGIIVYRGLYFGLYDS+
Sbjct: 3 LANDAKASKGGGERQFNGLVDVYRKTLKTDGIAGLYRGFNISCVGIIVYRGLYFGLYDSL 182
Query: 983 KPVVLTGNLQDNFFASFALGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSSLDAFQQ 1162
KPV+LTG LQDNFFASFALGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSS DAFQQ
Sbjct: 183 KPVLLTGTLQDNFFASFALGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSSFDAFQQ 362
Query: 1163 ILKKEGPKSLFKGAGANILRAIAGAGVLSGYDQLQILFFGKKYGSGGA*MEKYDDEQEQC 1342
ILKKEG KSLFKGAGANILRAIAGAGVLSGYDQLQILFFGKKYGSGGA* + DDEQ++
Sbjct: 363 ILKKEGAKSLFKGAGANILRAIAGAGVLSGYDQLQILFFGKKYGSGGA*SGRNDDEQQES 542
Query: 1343 VFP 1351
P
Sbjct: 543 CVP 551
Score = 285 bits (617), Expect(2) = 1e-081
Identities = 127/163 (77%), Positives = 133/163 (81%)
Frame = -2 / -3
Query: 1332 CSSSYFSI*APPEPYFFPKKRIWSWS*PESTXXXXXXXXXXXXXXLNRDLGPSFLRICWN 1153
CSSS+ +*APPEPYF PKKRI SWS*PEST L RDL PSF RICW
Sbjct: 532 CSSSFLPL*APPEPYFLPKKRI*SWS*PESTPAPAMARRMLAPAPLKRDLAPSFFRICWK 353
Query: 1152 ASKELLYLTASPDVIIILLRTVSMG*DARPAPLVISQPRAKLAKKLSWRLPVRTTGLMES 973
AS ELLYLTASP+VIIILLRTVSMG*DARPAPLVI+QPRAKLAKKLSWR+PVR TG ES
Sbjct: 352 ASNELLYLTASPEVIIILLRTVSMG*DARPAPLVINQPRAKLAKKLSWRVPVRRTGFRES 173
Query: 972 *SPKYRPR*TMIPTQEMLNPR*SPAIPSDLSVLR*TSTSPLNC 844
+PKYRPR*TMIPTQEMLNPR*SPAIPS L VLR*TST PLNC
Sbjct: 172 YNPKYRPR*TMIPTQEMLNPR*SPAIPSVLRVLR*TSTRPLNC 44
Score = 269 bits (581), Expect = 1e-072
Identities = 120/186 (64%), Positives = 144/186 (77%)
Frame = -1 / -2
Query: 1360 GKDREHTLLLFVIIFLHLSTAGAVXXXXXXXXXLVIARKHTSTSNGTKNVSTSTLEQGLG 1181
G+ TLLLFVII L TA AV LVIARKHTSTSNGT++V TSTLE+GL
Sbjct: 560 GRTGNATLLLFVIISSTLGTA*AVLLAEEEDLKLVIARKHTSTSNGTEDVGTSTLEEGLS 381
Query: 1180 PFLLKNLLERVQGALVLDSLTRCHHHPSADGIDGVRCKTSTISDQPTQSETGKEIVLEVA 1001
LL++LLE ++ ALVLD +R HHHPSADG++GVRCKT T+ DQPT+S+ GKE+VLE A
Sbjct: 380 TLLLQDLLESIE*ALVLDGFSRGHHHPSADGVNGVRCKTCTVGDQPTKSKAGKEVVLESA 201
Query: 1000 SKDNWLDGIIEPKVQTTINNDSNTGDVKSTVKPSNTIRLECLAVDIDKPIELPFTSSLGS 821
S++NWL II+ KVQTT++NDSNTGDV+STVKPSNTI LE LAVDI+K IELP TS+L
Sbjct: 200 SEENWLQRIIQSKVQTTVDNDSNTGDVESTVKPSNTICLESLAVDINKAIELPLTSTLRC 21
Query: 820 LRIISQ 803
L +I Q
Sbjct: 20 LSVIGQ 3
Score = 213 bits (460), Expect(2) = 5e-066
Identities = 84/127 (66%), Positives = 93/127 (73%)
Frame = +1 / +2
Query: 820 GCQGRR*KAVQWACRCLPQDTQV*WYCWALPWI*HLLCWNHCLSWSVLWAL*FHQASCPY 999
G G R*+AVQW C CLPQD+Q WYCWALPWI HLLCWNHCL WSVLW +*F +AS P+
Sbjct: 20 GI*GWR*EAVQWPC*CLPQDSQDRWYCWALPWIQHLLCWNHCLPWSVLWIV*FSEASSPH 199
Query: 1000 WQPPGQFLCQFRSGLADH*WCWSCILPHRYRPQKDDDDIW*GCQVQELLGRVPADS*EGR 1179
W PGQ LCQ S L DH C SCILPH +RPQKDDDD+W QVQEL+ PAD EG
Sbjct: 200 WHSPGQLLCQLCSWLVDHQRCRSCILPH*HRPQKDDDDLWRSRQVQELIRCFPADPEEGG 379
Query: 1180 AQVPVQG 1200
VP+QG
Sbjct: 380 C*VPLQG 400
Score = 198 bits (426), Expect(2) = 2e-052
Identities = 88/132 (66%), Positives = 97/132 (73%)
Frame = -3 / -1
Query: 1199 P*TGTWALPS*ESAGTRPRSSCT*QPHQMSSSSFCGRYRWGKMQDQHH**SANPERNWQR 1020
P* GT PS SAG SSCT*+ Q SSSSFCGR +WGKMQD H *S N E++WQR
Sbjct: 399 P*RGT*HPPSSGSAGKHRMSSCT*RLLQRSSSSFCGRCQWGKMQDLHRW*STNQEQSWQR 220
Query: 1019 NCPGGCQ*GQLA*WNHRAQSTDHDKQ*FQHRRC*IHGKAQQYHQT*VSCGRHRQAH*TAF 840
+CPG CQ*G+LA NH QSTDH +Q*FQHRRC*IHGKAQQYH +* SCGRH+Q H*TA
Sbjct: 219 SCPGECQ*GELASENHTIQSTDHGRQ*FQHRRC*IHGKAQQYHLS*ESCGRHQQGH*TAS 40
Query: 839 HLLPWQPSHH*P 804
HL P P H P
Sbjct: 39 HLHP*MP*RHWP 4
Score = 156 bits (335), Expect(2) = 1e-040
Identities = 88/133 (66%), Positives = 91/133 (68%)
Frame = +3 / +1
Query: 801 GWLMMRRLPREEVKGSSMGLSMSTARHSSLMVLLGFTVDLTSPVLESLFIVVCTLGSMIP 980
GW M R R EV+GSSM L MSTAR S MVLLGFTVD TSPVLESL VVCTL MI
Sbjct: 1 GWPMTLRHLRVEVRGSSMALLMSTARLSRQMVLLGFTVDSTSPVLESLSTVVCTLDCMIL 180
Query: 981 SSQLSLLATSRTISLPVSLWVG*SLMVLVLHLTPSIPSAEG***HLVRLSSTRAPWTRSS 1160
SQ S LA SRT SLP L VG*S V VLHLTP PSAEG*** L + SSTRA SS
Sbjct: 181 *SQFSSLALSRTTSLPALLLVG*SPTVQVLHLTPLTPSAEG***PLEKPSSTRAHSMLSS 360
Query: 1161 RFLRRKGPSPCSR 1199
R RR+ SP SR
Sbjct: 361 RS*RRRVLSPSSR 399
Score = 54.2 bits (112), Expect(2) = 5e-066
Identities = 24/30 (80%), Positives = 26/30 (86%)
Frame = +1 / +2
Query: 1243 AFWL*PAPDPLLRKEVRLRRCLNGEI**RT 1332
AFWL*PA DPLLR+EVRLRRCL + **RT
Sbjct: 443 AFWL*PASDPLLRQEVRLRRCLEWKK**RT 532
Score = 34.5 bits (69), Expect(2) = 1e-081
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = -2 / -2
Query: 1449 SKTNCWKNPPST*IINF 1399
+KT CW+NPPST II F
Sbjct: 647 TKTECWENPPSTEIIAF 597
Score = 26.3 bits (51), Expect(2) = 1e-040
Identities = 13/39 (33%), Positives = 16/39 (41%)
Frame = +3 / +1
Query: 1251 AMTXXXXXXXXXXTAPAVLKWRNMMTNKSSVCSRSFPIR 1367
AMT TA AV + MMTN V P++
Sbjct: 451 AMTSFRSSSSARSTAQAVPRVEEMMTNNKRVAFPVLPLQ 567
Score = 24.0 bits (46), Expect(2) = 2e-052
Identities = 11/34 (32%), Positives = 15/34 (44%)
Frame = -3 / -1
Query: 1343 HTALVRHHISPFKHXXXXXXXXXXXXXXGHSQKA 1242
++ +VRHH +H GHSQKA
Sbjct: 543 NSLVVRHHFFHSRHRLSRTSCRRRGSEAGHSQKA 442
Score = 23.1 bits (44), Expect(2) = e-111
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = +1 / +3
Query: 1342 CVPGPFQSGSG 1374
CVP P SGSG
Sbjct: 543 CVPSPAPSGSG 575
>gb|AI978372.1|AI978372 CDO507.R Oat Etiolated Leaf cDNA Library Avena sativa cDNA clone
CDO507, mRNA sequence
Length = 394
Score = 107 bits (229), Expect = 4e-024
Identities = 44/64 (68%), Positives = 53/64 (82%)
Frame = +2 / +3
Query: 146 MADQANQPTVLHKLGGQFHLRSIISEGVRARNICPSVSSYERRFATRNYMTQSLWGPSMS 325
MADQANQP+V+ K GGQFHL S S VRARN+CPSVS+Y+RRF T +YMTQSL+ P+MS
Sbjct: 117 MADQANQPSVVQKFGGQFHLGSSFSGVVRARNMCPSVSAYDRRFTTSSYMTQSLYVPTMS 296
Query: 326 VSGG 337
+GG
Sbjct: 297 FNGG 308
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 18,020,158
Number of Sequences: 8143
Number of extensions: 263106
Number of successful extensions: 1144
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 611335468
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)