TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.467
(758 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818898.1|CN818898 HRO4473_B10_XXXZS5 Lib AA070E1X Aven... 304 1e-083
gb|CN816629.1|CN816629 HRO4523_A05_B09ZS5 Lib AA071E1X Aven... 215 5e-057
gb|CN815400.1|CN815400 HRO4509_C03_E05ZS5 Lib AA071E1X Aven... 208 8e-055
gb|CN815715.1|CN815715 HRO4514_G02_M04ZS5 Lib AA071E1X Aven... 127 2e-030
>gb|CN818898.1|CN818898 HRO4473_B10_XXXZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4473_B10_XXX, mRNA sequence
Length = 683
Score = 304 bits (658), Expect = 1e-083
Identities = 133/138 (96%), Positives = 135/138 (97%)
Frame = +2 / +3
Query: 89 MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTE 268
MARTKQTARKSTGGKAPRKQLATKAARKSAP TGGVKKPHR+RPGTVALREIRKYQKSTE
Sbjct: 60 MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 239
Query: 269 LLIRKLPFQRLVREIAQDFKTDLRFQSSAVAALQEASEAYLVGLFEDTNLCAIHAKRVTI 448
LLIRKLPFQRLVREIAQDFKTDLRFQS AV ALQEA+EAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 240 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 419
Query: 449 MPKDIQLARRIRGERA*A 502
MPKDIQLARRIRGERA*A
Sbjct: 420 MPKDIQLARRIRGERA*A 473
Score = 255 bits (552), Expect = 5e-069
Identities = 113/138 (81%), Positives = 114/138 (82%)
Frame = -3 / -2
Query: 501 A*ALSPRIRRASWMSLGMMVTRLA*MAQRLVSSKSPTRXXXXXXXXXXXAELWKRRSVLK 322
A*A SPR+RRASWMSLGMMVTRLA MA RLVSSKSPTR A LWKRRSVLK
Sbjct: 472 A*ARSPRMRRASWMSLGMMVTRLAWMAHRLVSSKSPTRYASAASWSASTAWLWKRRSVLK 293
Query: 321 SWAISRTRRWNGSLRISSSVLFWYLRISRSATVPGRKRWGFFTPPVAGADLRAALVASCF 142
SWAISRTRRW GSLRISSSVLFWYLRISRSATVPG RWGFFTPPV GAD AALVASCF
Sbjct: 292 SWAISRTRRWKGSLRISSSVLFWYLRISRSATVPGL*RWGFFTPPVVGADFLAALVASCF 113
Query: 141 LGALPPVDFLAVCFVRAI 88
LGALPPVD AVC VRAI
Sbjct: 112 LGALPPVDLRAVCLVRAI 59
Score = 111 bits (238), Expect = 1e-025
Identities = 48/81 (59%), Positives = 55/81 (67%)
Frame = +1 / +2
Query: 88 DGPHEADGEEVHWRQGAKEAAGHQGGAQVCPGDRRSEEXXXXXXXXXXXXGDPQVPEEHG 267
DGP++AD +VH RQG +EAA +QG +VC RR EE DPQVPEEHG
Sbjct: 59 DGPYQADRPQVHRRQGPQEAARNQGCEEVCSHHRRREEAPPLQARHGGAPRDPQVPEEHG 238
Query: 268 AADPQAAVPAPGA*DRPGLQD 330
AADPQAA+PAPG DRPGLQD
Sbjct: 239 AADPQAALPAPGPRDRPGLQD 301
>gb|CN816629.1|CN816629 HRO4523_A05_B09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4523_A05_B09, mRNA sequence
Length = 550
Score = 215 bits (465), Expect = 5e-057
Identities = 94/98 (95%), Positives = 96/98 (97%)
Frame = +2 / +3
Query: 206 HRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVAALQEASEA 385
HR+RPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQS AV ALQEA+EA
Sbjct: 3 HRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEA 182
Query: 386 YLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA* 499
YLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*
Sbjct: 183 YLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA* 296
Score = 148 bits (318), Expect = 9e-037
Identities = 72/104 (69%), Positives = 73/104 (70%)
Frame = -3 / -1
Query: 519 HLHRDDA*ALSPRIRRASWMSLGMMVTRLA*MAQRLVSSKSPTRXXXXXXXXXXXAELWK 340
H R *ALSPRI AS MSLGMMVT LA MA RLVSSK PTR A LWK
Sbjct: 316 HSKRQST*ALSPRILLAS*MSLGMMVTLLAWMAHRLVSSKRPTRYASAAS*RASTAWLWK 137
Query: 339 RRSVLKSWAISRTRRWNGSLRISSSVLFWYLRISRSATVPGRKR 208
RRSVLKSWAIS T WNGS +SSSVLFWYLRISR ATVPGR R
Sbjct: 136 RRSVLKSWAISLTSLWNGSFLMSSSVLFWYLRISRRATVPGR*R 5
>gb|CN815400.1|CN815400 HRO4509_C03_E05ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_C03_E05, mRNA sequence
Length = 516
Score = 208 bits (449), Expect = 8e-055
Identities = 92/95 (96%), Positives = 93/95 (97%)
Frame = +2 / +3
Query: 215 RPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVAALQEASEAYLV 394
RPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQS AV ALQEA+EAYLV
Sbjct: 3 RPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLV 182
Query: 395 GLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA* 499
GLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*
Sbjct: 183 GLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA* 287
Score = 143 bits (307), Expect = 3e-035
Identities = 69/95 (72%), Positives = 70/95 (73%)
Frame = -3 / -3
Query: 498 *ALSPRIRRASWMSLGMMVTRLA*MAQRLVSSKSPTRXXXXXXXXXXXAELWKRRSVLKS 319
*ALSPRI AS MSLGMMVT LA MA RLVSSK PTR A LWKRRSVLKS
Sbjct: 286 *ALSPRILLAS*MSLGMMVTLLAWMAHRLVSSKRPTRYASAAS*RASTAWLWKRRSVLKS 107
Query: 318 WAISRTRRWNGSLRISSSVLFWYLRISRSATVPGR 214
WAIS T WNGS +SSSVLFWYLRISR ATVPGR
Sbjct: 106 WAISLTSLWNGSFLMSSSVLFWYLRISRRATVPGR 2
>gb|CN815715.1|CN815715 HRO4514_G02_M04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_G02_M04, mRNA sequence
Length = 682
Score = 127 bits (273), Expect = 2e-030
Identities = 56/59 (94%), Positives = 57/59 (96%)
Frame = +2 / +3
Query: 323 FKTDLRFQSSAVAALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA* 499
FKTDLRFQS AV ALQEA+EAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*
Sbjct: 15 FKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA* 191
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,090,925
Number of Sequences: 8143
Number of extensions: 71215
Number of successful extensions: 771
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 254423642
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)