TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.426
(1721 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815233.1|CN815233 HRO4507_D04_H07ZS5 Lib AA071E1X Aven... 378 e-105
gb|AI978372.1|AI978372 CDO507.R Oat Etiolated Leaf cDNA Lib... 113 8e-027
>gb|CN815233.1|CN815233 HRO4507_D04_H07ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4507_D04_H07, mRNA sequence
Length = 654
Score = 378 bits (819), Expect = e-105
Identities = 164/205 (80%), Positives = 173/205 (84%)
Frame = -2 / +3
Query: 964 LANXXXXXXXXXDRQFNGLVDVYRKTLKSDGIAGLYRGFNISCVGIIVYRGLYFGLYDSI 785
LAN +RQFNGLVDVYRKTLK+DGIAGLYRGFNISCVGIIVYRGLYFGLYDS+
Sbjct: 3 LANDAKASKGGGERQFNGLVDVYRKTLKTDGIAGLYRGFNISCVGIIVYRGLYFGLYDSL 182
Query: 784 KPVVLTGSLQDNFFASFALGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSSLDAFQQ 605
KPV+LTG+LQDNFFASFALGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSS DAFQQ
Sbjct: 183 KPVLLTGTLQDNFFASFALGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSSFDAFQQ 362
Query: 604 ILKKEGPKSLFKGAGANILRAIAGAGVLSGYDQLQILFFGKKYGSGGA*MEKNDDEQEQF 425
ILKKEG KSLFKGAGANILRAIAGAGVLSGYDQLQILFFGKKYGSGGA* +NDDEQ++
Sbjct: 363 ILKKEGAKSLFKGAGANILRAIAGAGVLSGYDQLQILFFGKKYGSGGA*SGRNDDEQQES 542
Query: 424 APGPAQSGSGQVFAFHIEEINNLCR 350
F F + NN CR
Sbjct: 543 CVPSPAPSGSGNFIFLTLKGNNFCR 617
Score = 265 bits (574), Expect(2) = 1e-076
Identities = 119/152 (78%), Positives = 125/152 (82%)
Frame = +3 / -3
Query: 456 I*APPEPYFFPKKRIWSWS*PESTXXXXXXXXXXXXXXXNRDLGPSFLRICWNASNELLY 635
+*APPEPYF PKKRI SWS*PEST RDL PSF RICW ASNELLY
Sbjct: 511 L*APPEPYFLPKKRI*SWS*PESTPAPAMARRMLAPAPLKRDLAPSFFRICWKASNELLY 332
Query: 636 LTASPEVIIILLRTVSMG*DARPAPLVISQPRAKLAKKLSWRLPVRTTGLIES*SPKYRP 815
LTASPEVIIILLRTVSMG*DARPAPLVI+QPRAKLAKKLSWR+PVR TG ES +PKYRP
Sbjct: 331 LTASPEVIIILLRTVSMG*DARPAPLVINQPRAKLAKKLSWRVPVRRTGFRESYNPKYRP 152
Query: 816 R*TIIPTQDMLNPR*SPAIPSDLSVLR*TSTR 911
R*T+IPTQ+MLNPR*SPAIPS L VLR*TSTR
Sbjct: 151 R*TMIPTQEMLNPR*SPAIPSVLRVLR*TSTR 56
Score = 247 bits (533), Expect = 5e-066
Identities = 105/177 (59%), Positives = 117/177 (66%)
Frame = +1 / -1
Query: 433 LVRHHFSPFKHXXXXXXXXXXXXXXXXXQKAHQRQQWHEEC*HQHP*TGTWGLPS*GSAG 612
+VRHHF +H QKAHQ QQWH C HQHP* GT PS GSAG
Sbjct: 534 VVRHHFFHSRHRLSRTSCRRRGSEAGHSQKAHQHQQWHGGCWHQHP*RGT*HPPSSGSAG 355
Query: 613 MRPMSSCT*QPHQKSSSSFCGQYQWGKMQDQHRW*SANPEQNWQRSCPGGCQ*GQLA**N 792
MSSCT*+ Q+SSSSFCG+ QWGKMQD HRW*S N EQ+WQRSCPG CQ*G+LA N
Sbjct: 354 KHRMSSCT*RLLQRSSSSFCGRCQWGKMQDLHRW*STNQEQSWQRSCPGECQ*GELASEN 175
Query: 793 RKVRNTGHDKQ*FQHKIC*IHGKAQQYHQI*VSCGRHPQDH*IVYRLLPWQPWHHWP 963
+++T H +Q*FQH+ C*IHGKAQQYH * SCGRH Q H* L P P HWP
Sbjct: 174 HTIQSTDHGRQ*FQHRRC*IHGKAQQYHLS*ESCGRHQQGH*TASHLHP*MP*RHWP 4
Score = 242 bits (523), Expect = 1e-064
Identities = 106/164 (64%), Positives = 126/164 (76%)
Frame = +2 / -2
Query: 428 LLLFVIIFLHLSTAGAXXXXXXXXXXXXXARKHTSASNGTKNVSTSTLEQGLGAFLLKDL 607
LLLFVII L TA A ARKHTS SNGT++V TSTLE+GL LL+DL
Sbjct: 539 LLLFVIISSTLGTA*AVLLAEEEDLKLVIARKHTSTSNGTEDVGTSTLEEGLSTLLLQDL 360
Query: 608 LECVQ*ALVLDSLTRSHHHPSADSINGVRCKTSTVGDQPTQSKTGKEVVLEAASEDNWLD 787
LE ++*ALVLD +R HHHPSAD +NGVRCKT TVGDQPT+SK GKEVVLE+ASE+NWL
Sbjct: 359 LESIE*ALVLDGFSRGHHHPSADGVNGVRCKTCTVGDQPTKSKAGKEVVLESASEENWLQ 180
Query: 788 RIVKSEIQATINNNSNTRYVKSTVKPSNTIRFECLAVDIHKTIE 919
RI++S++Q T++N+SNT V+STVKPSNTI E LAVDI+K IE
Sbjct: 179 RIIQSKVQTTVDNDSNTGDVESTVKPSNTICLESLAVDINKAIE 48
Score = 208 bits (449), Expect(3) = 2e-069
Identities = 79/118 (66%), Positives = 87/118 (73%)
Frame = -1 / +2
Query: 920 IQWSCGCLPQDTQI*WYCWALPWI*HILCWNYCLSWPVFRTLRFYQASCPHWQPPGQLLC 741
+QW C CLPQD+Q WYCWALPWI H+LCWN+CL W V + F +AS PHW PGQLLC
Sbjct: 47 VQWPC*CLPQDSQDRWYCWALPWIQHLLCWNHCLPWSVLWIV*FSEASSPHWHSPGQLLC 226
Query: 740 QFCSGLADHQRCWSCILPH*YCPQKDDDDFW*GCQVQELIGRIPADP*EGRPQVPVQG 567
Q CS L DHQRC SCILPH*+ PQKDDDD W QVQELI PADP EG VP+QG
Sbjct: 227 QLCSWLVDHQRCRSCILPH*HRPQKDDDDLWRSRQVQELIRCFPADPEEGGC*VPLQG 400
Score = 170 bits (365), Expect = 8e-043
Identities = 98/177 (55%), Positives = 103/177 (58%)
Frame = -3 / +1
Query: 966 GWPMMPRLPREEAIDNSMVLWMSTARHSNLMVLLGFTVDLTYLVLELLFIVACISDFTIX 787
GWPM R R E +SM L MSTAR S MVLLGFTVD T VLE L V C D I
Sbjct: 1 GWPMTLRHLRVEVRGSSMALLMSTARLSRQMVLLGFTVDSTSPVLESLSTVVCTLDCMIL 180
Query: 786 XXXXXXXXXXXXXXLPVLLWVG*SPTVLVLHLTPLILSAEG***LLVRLSSTRAHWTHSS 607
LP LL VG*SPTV VLHLTPL SAEG*** L + SSTRAH SS
Sbjct: 181 *SQFSSLALSRTTSLPALLLVG*SPTVQVLHLTPLTPSAEG***PLEKPSSTRAHSMLSS 360
Query: 606 RSLRRKAPSPCSRVLVLTFFVPLLALVCFLAXXXXXXXXXXXXTAPAVLKWRKMMTN 436
RS RR+ SP SRVLV T VPLL LVCFLA TA AV + +MMTN
Sbjct: 361 RS*RRRVLSPSSRVLVPTSSVPLLVLVCFLAMTSFRSSSSARSTAQAVPRVEEMMTN 531
Score = 50.1 bits (103), Expect(3) = 2e-069
Identities = 20/26 (76%), Positives = 22/26 (84%)
Frame = -1 / +2
Query: 524 AFWLRPAPDPLLRKEVRLRRCLNGEK 447
AFWL PA DPLLR+EVRLRRCL +K
Sbjct: 443 AFWL*PASDPLLRQEVRLRRCLEWKK 520
Score = 39.6 bits (80), Expect(3) = 2e-069
Identities = 18/43 (41%), Positives = 23/43 (53%)
Frame = -2 / +3
Query: 448 NDDEQEQFAPGPAQSGSGQVFAFHIEEINNLCRRIFPTFVLGG 320
+D++QE P PA SGSG ++ N IFPTF GG
Sbjct: 522 DDEQQESCVPSPAPSGSGNFIFLTLKGNNFCRGWIFPTFGFGG 650
Score = 37.3 bits (75), Expect(2) = 1e-076
Identities = 18/43 (41%), Positives = 24/43 (55%)
Frame = +2 / -2
Query: 320 SAQNECWKNSPT*IINFFDMKGKNLTRS*LGWTGSELLLFVII 448
S + ECW+N P+ I F +TRS* G TG+ LL +I
Sbjct: 650 STKTECWENPPSTEIIAFQR*KDKVTRS*RGRTGNATLLLFVI 522
>gb|AI978372.1|AI978372 CDO507.R Oat Etiolated Leaf cDNA Library Avena sativa cDNA clone
CDO507, mRNA sequence
Length = 394
Score = 113 bits (241), Expect(2) = 8e-027
Identities = 47/71 (66%), Positives = 56/71 (78%)
Frame = -2 / +3
Query: 1621 MADQANQPTVLHKLGGQFHLSSSFSEGVRARNICPSFSSYERRFATRNYMTQSLWGPSMS 1442
MADQANQP+V+ K GGQFHL SSFS VRARN+CPS S+Y+RRF T +YMTQSL+ P+MS
Sbjct: 117 MADQANQPSVVQKFGGQFHLGSSFSGVVRARNMCPSVSAYDRRFTTSSYMTQSLYVPTMS 296
Query: 1441 VSGGINVPVMP 1409
+GG V P
Sbjct: 297 FNGGSMVSSSP 329
Score = 97.3 bits (206), Expect = 7e-021
Identities = 45/75 (60%), Positives = 51/75 (68%)
Frame = +3 / -1
Query: 1407 VGITGTLMPPLTDIEGPQRLWVM*FLVANLLS*DEKEGQMLRARTPSEKLELRWNWPPSL 1586
+G T++PPL DI G RL VM* LV LS* + EG MLRART EKLE RWN PP+
Sbjct: 331 IGEEDTMLPPLKDIVGT*RL*VM*LLVVKRLS*ADTEGHMLRARTTPEKLEPRWNCPPNF 152
Query: 1587 *RTVGWLAWSAITTS 1631
* T GWLAWSA+ S
Sbjct: 151 *TTDGWLAWSAMVLS 107
Score = 23.1 bits (44), Expect(2) = 8e-027
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = -2 / +3
Query: 1417 VMPTPLFANAPAEK 1376
V +P+F +APAEK
Sbjct: 315 VSSSPIFXSAPAEK 356
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 18,801,754
Number of Sequences: 8143
Number of extensions: 276786
Number of successful extensions: 1226
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 634155456
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)