TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.36
(834 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818898.1|CN818898 HRO4473_B10_XXXZS5 Lib AA070E1X Aven... 317 1e-087
gb|CN816629.1|CN816629 HRO4523_A05_B09ZS5 Lib AA071E1X Aven... 225 7e-060
gb|CN815400.1|CN815400 HRO4509_C03_E05ZS5 Lib AA071E1X Aven... 219 4e-058
gb|CN815715.1|CN815715 HRO4514_G02_M04ZS5 Lib AA071E1X Aven... 135 8e-033
>gb|CN818898.1|CN818898 HRO4473_B10_XXXZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4473_B10_XXX, mRNA sequence
Length = 683
Score = 317 bits (687), Expect = 1e-087
Identities = 139/140 (99%), Positives = 139/140 (99%)
Frame = +2 / +3
Query: 131 MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 310
MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE
Sbjct: 60 MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTE 239
Query: 311 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 490
LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI
Sbjct: 240 LLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTI 419
Query: 491 MPKDIQLARRIRGERA*AYL 550
MPKDIQLARRIRGERA*A L
Sbjct: 420 MPKDIQLARRIRGERA*AIL 479
Score = 251 bits (543), Expect = 1e-067
Identities = 110/141 (78%), Positives = 113/141 (80%)
Frame = -1 / -2
Query: 552 ARYAYARSPRMRRASWMSLGMMVTRFAWMAQRLVSSNRPXXXXXXXXXXXXXXWLWNRRS 373
++ A ARSPRMRRASWMSLGMMVTR AWMA RLVSS P WLW RRS
Sbjct: 481 SKMA*ARSPRMRRASWMSLGMMVTRLAWMAHRLVSSKSPTRYASAASWSASTAWLWKRRS 302
Query: 372 VLKS*AISRTRRWKGNFRISSSVLFWYLRISRSATVPGLYLWGFFTPPVVGADFLAALVA 193
VLKS AISRTRRWKG+ RISSSVLFWYLRISRSATVPGL WGFFTPPVVGADFLAALVA
Sbjct: 301 VLKSWAISRTRRWKGSLRISSSVLFWYLRISRSATVPGL*RWGFFTPPVVGADFLAALVA 122
Query: 192 SCLRGALPPVDLRAVCFVRAI 130
SC GALPPVDLRAVC VRAI
Sbjct: 121 SCFLGALPPVDLRAVCLVRAI 59
Score = 158 bits (340), Expect(2) = 2e-048
Identities = 72/98 (73%), Positives = 78/98 (79%)
Frame = -3 / -1
Query: 541 LRALAADAAGELDVFGHDGDALCVDGAEVGVLEQADEVGLGGFLEGQDGVALEPQIRLEV 362
L A +AAGELDV GHDGDAL VDGA+VGVLE+ EV L G LE Q GVALE ++ LEV
Sbjct: 470 LGTFATNAAGELDVLGHDGDALGVDGAQVGVLEEPHEVRLRGLLERQHGVALEAEVGLEV 291
Query: 361 LSDLTDQALEGQLPDQQLRALLVLADLAQRHRPGPVPV 248
L DL DQALEGQL DQQLRALLVLADLA+RHR GPV V
Sbjct: 290 LGDLADQALEGQLADQQLRALLVLADLAERHRAGPVAV 177
Score = 154 bits (331), Expect(2) = 1e-044
Identities = 63/81 (77%), Positives = 69/81 (85%)
Frame = +1 / +2
Query: 130 DGSYEADRPQVHRRQGPAQAARHQGGEEVGADDRRREEAPQVQARDGGAARDPQVPEEHG 309
DG Y+ADRPQVHRRQGP +AAR+QG EEV + RRREEAP +QAR GGA RDPQVPEEHG
Sbjct: 59 DGPYQADRPQVHRRQGPQEAARNQGCEEVCSHHRRREEAPPLQARHGGAPRDPQVPEEHG 238
Query: 310 AADPEVALPAPGP*DRSGLQD 372
AADP+ ALPAPGP DR GLQD
Sbjct: 239 AADPQAALPAPGPRDRPGLQD 301
Score = 73.9 bits (155), Expect(2) = 1e-022
Identities = 30/35 (85%), Positives = 30/35 (85%)
Frame = -2 / -3
Query: 371 S*SPERSHGPGAGRATSGSAAPCSSGTCGSRAAPP 267
S*SP RS GPGAGRA GSAAPCSSGTCGSR APP
Sbjct: 300 S*SPGRSRGPGAGRAACGSAAPCSSGTCGSRGAPP 196
Score = 51.5 bits (106), Expect(2) = 2e-048
Identities = 20/25 (80%), Positives = 20/25 (80%)
Frame = -3 / -3
Query: 100 PCLRAACAGPCLRWTCGRSASYEPS 26
P LRAA GPCLRWTCGRSA Y PS
Sbjct: 132 PWLRAASWGPCLRWTCGRSAWYGPS 58
Score = 49.6 bits (102), Expect(2) = 1e-022
Identities = 20/27 (74%), Positives = 23/27 (85%)
Frame = -2 / -2
Query: 107 ISSLPASCLRGALPPVDLRAVCFVRAI 27
+++L ASC GALPPVDLRAVC VRAI
Sbjct: 139 LAALVASCFLGALPPVDLRAVCLVRAI 59
Score = 40.5 bits (82), Expect(2) = 1e-044
Identities = 18/28 (64%), Positives = 19/28 (67%)
Frame = +3 / +1
Query: 351 RSLRTSRRICGSRATPSWPSRKPPRPTS 434
RS RTSR SRATP W SR+P R TS
Sbjct: 280 RSPRTSRPTSASRATPCWRSRRPRRRTS 363
>gb|CN816629.1|CN816629 HRO4523_A05_B09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4523_A05_B09, mRNA sequence
Length = 550
Score = 225 bits (486), Expect = 7e-060
Identities = 98/98 (100%), Positives = 98/98 (100%)
Frame = +2 / +3
Query: 248 HRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEA 427
HRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEA
Sbjct: 3 HRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEA 182
Query: 428 YLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA* 541
YLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*
Sbjct: 183 YLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA* 296
Score = 127 bits (271), Expect = 3e-030
Identities = 60/93 (64%), Positives = 63/93 (67%)
Frame = -1 / -1
Query: 537 ARSPRMRRASWMSLGMMVTRFAWMAQRLVSSNRPXXXXXXXXXXXXXXWLWNRRSVLKS* 358
A SPR+ AS MSLGMMVT AWMA RLVSS RP WLW RRSVLKS
Sbjct: 292 ALSPRILLAS*MSLGMMVTLLAWMAHRLVSSKRPTRYASAAS*RASTAWLWKRRSVLKSW 113
Query: 357 AISRTRRWKGNFRISSSVLFWYLRISRSATVPG 259
AIS T W G+F +SSSVLFWYLRISR ATVPG
Sbjct: 112 AISLTSLWNGSFLMSSSVLFWYLRISRRATVPG 14
Score = 96.8 bits (205), Expect = 4e-021
Identities = 47/98 (47%), Positives = 64/98 (65%)
Frame = -3 / -3
Query: 541 LRALAADAAGELDVFGHDGDALCVDGAEVGVLEQADEVGLGGFLEGQDGVALEPQIRLEV 362
L LA D +L+V GHDG L +DGA+VGVL++ +V L L+ + VALE QI LE+
Sbjct: 296 LSPLATDPPSQLNVLGHDGHPLGMDGAQVGVLKETHQVRLCSLLKSKHCVALETQISLEI 117
Query: 361 LSDLTDQALEGQLPDQQLRALLVLADLAQRHRPGPVPV 248
L +L ++ LE +LP +QL ALLVLADL + + V V
Sbjct: 116 LGNLPNKPLEWELPYEQLSALLVLADLTKSNSSRAVAV 3
>gb|CN815400.1|CN815400 HRO4509_C03_E05ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_C03_E05, mRNA sequence
Length = 516
Score = 219 bits (473), Expect = 4e-058
Identities = 97/101 (96%), Positives = 98/101 (97%)
Frame = +2 / +3
Query: 257 RPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLV 436
RPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLV
Sbjct: 3 RPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLV 182
Query: 437 GLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*AYLAPG 559
GLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA* L+ G
Sbjct: 183 GLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*VLLSLG 305
Score = 127 bits (271), Expect = 3e-030
Identities = 60/93 (64%), Positives = 63/93 (67%)
Frame = -1 / -3
Query: 537 ARSPRMRRASWMSLGMMVTRFAWMAQRLVSSNRPXXXXXXXXXXXXXXWLWNRRSVLKS* 358
A SPR+ AS MSLGMMVT AWMA RLVSS RP WLW RRSVLKS
Sbjct: 283 ALSPRILLAS*MSLGMMVTLLAWMAHRLVSSKRPTRYASAAS*RASTAWLWKRRSVLKSW 104
Query: 357 AISRTRRWKGNFRISSSVLFWYLRISRSATVPG 259
AIS T W G+F +SSSVLFWYLRISR ATVPG
Sbjct: 103 AISLTSLWNGSFLMSSSVLFWYLRISRRATVPG 5
Score = 99.1 bits (210), Expect = 8e-022
Identities = 46/97 (47%), Positives = 65/97 (67%)
Frame = -3 / -2
Query: 553 REIRLRALAADAAGELDVFGHDGDALCVDGAEVGVLEQADEVGLGGFLEGQDGVALEPQI 374
R+ L LA D +L+V GHDG L +DGA+VGVL++ +V L L+ + +ALE QI
Sbjct: 299 RQKHLSPLATDPPSQLNVLGHDGHPLGMDGAQVGVLKETHQVRLCSLLKSKHCMALETQI 120
Query: 373 RLEVLSDLTDQALEGQLPDQQLRALLVLADLAQRHRP 263
LE+L +L ++ LE +LP +QL ALLVLADL + + P
Sbjct: 119 SLEILGNLPNKPLEWELPYEQLSALLVLADLTKSNSP 9
>gb|CN815715.1|CN815715 HRO4514_G02_M04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_G02_M04, mRNA sequence
Length = 682
Score = 135 bits (290), Expect = 8e-033
Identities = 59/59 (100%), Positives = 59/59 (100%)
Frame = +2 / +3
Query: 365 FKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA* 541
FKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*
Sbjct: 15 FKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA* 191
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,467,686
Number of Sequences: 8143
Number of extensions: 112484
Number of successful extensions: 1255
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 284857092
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)