TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.29
(685 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Aven... 206 3e-054
gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Aven... 205 5e-054
gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Aven... 205 7e-054
gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Aven... 205 7e-054
gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Aven... 205 7e-054
gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Aven... 204 2e-053
gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Aven... 155 5e-039
gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Aven... 120 3e-028
gb|CN816143.1|CN816143 HRO4517_E01_I01ZS5 Lib AA071E1X Aven... 92 1e-023
>gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4516_H02_P04, mRNA sequence
Length = 472
Score = 206 bits (444), Expect = 3e-054
Identities = 91/92 (98%), Positives = 91/92 (98%)
Frame = +1 / +2
Query: 259 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAESAKLARYNKKPTITSREI 438
ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLA ESAKLARYNKKPTITSREI
Sbjct: 11 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREI 190
Query: 439 QTSVRLVLPGELAKHAVSEGTKAVTKFTSS*D 534
QTSVRLVLPGELAKHAVSEGTKAVTKFTSS*D
Sbjct: 191 QTSVRLVLPGELAKHAVSEGTKAVTKFTSS*D 286
Score = 149 bits (319), Expect = 6e-037
Identities = 69/95 (72%), Positives = 74/95 (77%)
Frame = -3 / -2
Query: 542 LQGS*DEVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSK 363
++ S*+EVN VTALVPSETACLASSPG TRRTEVWIS EVMVGF SK
Sbjct: 294 IRTS*EEVNLVTALVPSETACLASSPGSTRRTEVWISLEVMVGFLLYLASLADSPASFSK 115
Query: 362 MSLMKEFMMDMALDEMPMSGCTCFSTLKM*IL*VS 258
+SLMKEFMMDMAL+EMPMSG TC TLKM*IL*VS
Sbjct: 114 ISLMKEFMMDMALEEMPMSGWTCLRTLKM*IL*VS 10
>gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_A08_B15, mRNA sequence
Length = 655
Score = 205 bits (443), Expect = 5e-054
Identities = 92/96 (95%), Positives = 92/96 (95%)
Frame = +1 / +3
Query: 256 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAESAKLARYNKKPTITSRE 435
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLA ESAKLARYNKKPTITSRE
Sbjct: 81 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 260
Query: 436 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*DPCN 543
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* N
Sbjct: 261 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*SVPN 368
Score = 165 bits (355), Expect = 7e-042
Identities = 74/92 (80%), Positives = 76/92 (82%)
Frame = -3 / -1
Query: 530 *DEVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSLM 351
*DEVNFVTALVPS+TACLASSPGRTRRTEVWISREVMVGF SKMSLM
Sbjct: 355 *DEVNFVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLLYLASFADSPASFSKMSLM 176
Query: 350 KEFMMDMALDEMPMSGCTCFSTLKM*IL*VST 255
KEFMMDMAL+EMPMSGCTCF TLKM*IL VST
Sbjct: 175 KEFMMDMALEEMPMSGCTCFRTLKM*ILYVST 80
>gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4507_C01_F01, mRNA sequence
Length = 365
Score = 205 bits (442), Expect = 7e-054
Identities = 91/92 (98%), Positives = 91/92 (98%)
Frame = +1 / +3
Query: 256 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAESAKLARYNKKPTITSRE 435
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLA ESAKLARYNKKPTITSRE
Sbjct: 69 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 248
Query: 436 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 531
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 249 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 344
Score = 162 bits (348), Expect = 6e-041
Identities = 73/93 (78%), Positives = 77/93 (82%)
Frame = -3 / -2
Query: 533 S*DEVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSL 354
+*++VNFVTALVPSETACLASSPGRTRRTEVWISREVMVGF SKMSL
Sbjct: 346 T*EDVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFLL*RASLADSPASFSKMSL 167
Query: 353 MKEFMMDMALDEMPMSGCTCFSTLKM*IL*VST 255
MKEFMMDMAL+EMPMSGCTC TLKM*IL*VST
Sbjct: 166 MKEFMMDMALEEMPMSGCTCLRTLKM*IL*VST 68
>gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_B07_C14, mRNA
sequence
Length = 454
Score = 205 bits (442), Expect = 7e-054
Identities = 91/92 (98%), Positives = 91/92 (98%)
Frame = +1 / +1
Query: 256 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAESAKLARYNKKPTITSRE 435
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLA ESAKLARYNKKPTITSRE
Sbjct: 13 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 192
Query: 436 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 531
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 193 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 288
Score = 162 bits (348), Expect = 6e-041
Identities = 73/93 (78%), Positives = 77/93 (82%)
Frame = -3 / -3
Query: 533 S*DEVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSL 354
+*++VNFVTALVPSETACLASSPGRTRRTEVWISREVMVGF SKMSL
Sbjct: 290 T*EDVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFLL*RASLADSPASFSKMSL 111
Query: 353 MKEFMMDMALDEMPMSGCTCFSTLKM*IL*VST 255
MKEFMMDMAL+EMPMSGCTC TLKM*IL*VST
Sbjct: 110 MKEFMMDMALEEMPMSGCTCLRTLKM*IL*VST 12
>gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4408_D06_H12, mRNA
sequence
Length = 415
Score = 205 bits (442), Expect = 7e-054
Identities = 91/92 (98%), Positives = 91/92 (98%)
Frame = +1 / +2
Query: 256 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAESAKLARYNKKPTITSRE 435
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLA ESAKLARYNKKPTITSRE
Sbjct: 41 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 220
Query: 436 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 531
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 221 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 316
Score = 154 bits (331), Expect = 1e-038
Identities = 71/93 (76%), Positives = 76/93 (81%)
Frame = -3 / -2
Query: 533 S*DEVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSL 354
+*++VN VTALVPSETACLASSPGRTRRTEV ISREVMVGF SKMSL
Sbjct: 318 A*EDVNLVTALVPSETACLASSPGRTRRTEVCISREVMVGFLL*RASLADSPASFSKMSL 139
Query: 353 MKEFMMDMALDEMPMSGCTCFSTLKM*IL*VST 255
MKEFMMDMAL++MPMSGCTC STLKM*IL*VST
Sbjct: 138 MKEFMMDMALEDMPMSGCTCLSTLKM*IL*VST 40
>gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4527_G09_N17, mRNA sequence
Length = 412
Score = 204 bits (439), Expect = 2e-053
Identities = 90/92 (97%), Positives = 91/92 (98%)
Frame = +1 / +1
Query: 256 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAAESAKLARYNKKPTITSRE 435
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLA E+AKLARYNKKPTITSRE
Sbjct: 28 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 207
Query: 436 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 531
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 208 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 303
Score = 166 bits (356), Expect = 5e-042
Identities = 74/91 (81%), Positives = 76/91 (83%)
Frame = -3 / -3
Query: 527 DEVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSLMK 348
DEVN VTALVPS+TACLASSPGRTRRTEVWISREVMVGF SKMSLMK
Sbjct: 299 DEVNLVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLL*RASLAASPASFSKMSLMK 120
Query: 347 EFMMDMALDEMPMSGCTCFSTLKM*IL*VST 255
EFMMDMAL+EMPMSGCTCFSTLKM*IL*VST
Sbjct: 119 EFMMDMALEEMPMSGCTCFSTLKM*IL*VST 27
>gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_C10_F20, mRNA sequence
Length = 360
Score = 155 bits (334), Expect = 5e-039
Identities = 71/73 (97%), Positives = 71/73 (97%)
Frame = +1 / +2
Query: 313 GISSKAMSIMNSFINDIFEKLAAESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS 492
GISS AMSIMNSFINDIFEKLA ESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS
Sbjct: 11 GISS*AMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS 190
Query: 493 EGTKAVTKFTSS* 531
EGTKAVTKFTSS*
Sbjct: 191 EGTKAVTKFTSS* 229
Score = 113 bits (241), Expect = 3e-026
Identities = 52/75 (69%), Positives = 58/75 (77%)
Frame = -3 / -1
Query: 533 S*DEVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFXXXXXXXXXXXXXXSKMSL 354
+*++VN VTALVPSETACLASSPGRTRRTEV ISREVMVGF SKMSL
Sbjct: 231 A*EDVNLVTALVPSETACLASSPGRTRRTEVCISREVMVGFLL*RASLADSPASFSKMSL 52
Query: 353 MKEFMMDMALDEMPM 309
MKEFMMDMA ++MP+
Sbjct: 51 MKEFMMDMA*EDMPL 7
>gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_F01_K01, mRNA sequence
Length = 361
Score = 120 bits (256), Expect = 3e-028
Identities = 54/55 (98%), Positives = 54/55 (98%)
Frame = +1 / +3
Query: 367 EKLAAESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 531
EKLA ESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 12 EKLACESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 176
>gb|CN816143.1|CN816143 HRO4517_E01_I01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_E01_I01, mRNA sequence
Length = 384
Score = 91.8 bits (194), Expect(2) = 1e-023
Identities = 41/41 (100%), Positives = 41/41 (100%)
Frame = +1 / +2
Query: 409 KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 531
KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 56 KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 178
Score = 32.2 bits (64), Expect(2) = 1e-023
Identities = 12/18 (66%), Positives = 15/18 (83%)
Frame = +1 / +3
Query: 364 FEKLAAESAKLARYNKKP 417
FEKL+ ESAKLAR+ + P
Sbjct: 12 FEKLSGESAKLARFTRSP 65
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,249,642
Number of Sequences: 8143
Number of extensions: 24285
Number of successful extensions: 402
Number of sequences better than 1.0e-020: 9
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 226713985
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)