TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.287
(857 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Aven... 204 2e-053
gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Aven... 202 6e-053
gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Aven... 202 6e-053
gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Aven... 202 6e-053
gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Aven... 202 6e-053
gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Aven... 200 2e-052
gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Aven... 153 5e-038
gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Aven... 113 5e-026
gb|CN816143.1|CN816143 HRO4517_E01_I01ZS5 Lib AA071E1X Aven... 88 1e-022
>gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4527_G09_N17, mRNA sequence
Length = 412
Score = 204 bits (439), Expect = 2e-053
Identities = 89/89 (100%), Positives = 89/89 (100%)
Frame = +1 / +1
Query: 262 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 441
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE
Sbjct: 28 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 207
Query: 442 IQTSVRLVLPGELAKHAVSEGTKAVTKFT 528
IQTSVRLVLPGELAKHAVSEGTKAVTKFT
Sbjct: 208 IQTSVRLVLPGELAKHAVSEGTKAVTKFT 294
Score = 159 bits (342), Expect = 5e-040
Identities = 73/108 (67%), Positives = 80/108 (74%)
Frame = -1 / -3
Query: 596 TTTQGEHQHKRSLQRASVQA*EEVNLVTALVPSETACLASSPGSTRRTEVWISRDVMVGF 417
T T+ + Q + S+ +EVNLVTALVPS+TACLASSPG TRRTEVWISR+VMVGF
Sbjct: 362 THTETDRQQVPTQGGTSLDLYDEVNLVTALVPSDTACLASSPGRTRRTEVWISREVMVGF 183
Query: 416 XXXXXXXXXXXXXXSKMSLMKEFMMDMALEEMPMSGCTCFSTLKM*IL 273
SKMSLMKEFMMDMALEEMPMSGCTCFSTLKM*IL
Sbjct: 182 LL*RASLAASPASFSKMSLMKEFMMDMALEEMPMSGCTCFSTLKM*IL 39
Score = 97.3 bits (206), Expect = 3e-021
Identities = 38/43 (88%), Positives = 41/43 (95%)
Frame = -3 / -2
Query: 405 RELGGLAGQLLEDVVDEGVHDGHGLGGDADVRVHLLQHLEDVD 277
R+LGGLA + L+DVVDEGVHDGHGLGGDADVRVHLLQHLEDVD
Sbjct: 171 RQLGGLASEFLKDVVDEGVHDGHGLGGDADVRVHLLQHLEDVD 43
>gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4507_C01_F01, mRNA sequence
Length = 365
Score = 202 bits (436), Expect = 6e-053
Identities = 88/89 (98%), Positives = 89/89 (100%)
Frame = +1 / +3
Query: 262 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 441
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSRE
Sbjct: 69 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 248
Query: 442 IQTSVRLVLPGELAKHAVSEGTKAVTKFT 528
IQTSVRLVLPGELAKHAVSEGTKAVTKFT
Sbjct: 249 IQTSVRLVLPGELAKHAVSEGTKAVTKFT 335
Score = 152 bits (326), Expect = 9e-038
Identities = 68/92 (73%), Positives = 72/92 (78%)
Frame = -1 / -2
Query: 548 SVQA*EEVNLVTALVPSETACLASSPGSTRRTEVWISRDVMVGFXXXXXXXXXXXXXXSK 369
+ + *E+VN VTALVPSETACLASSPG TRRTEVWISR+VMVGF SK
Sbjct: 355 TTRT*EDVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFLL*RASLADSPASFSK 176
Query: 368 MSLMKEFMMDMALEEMPMSGCTCFSTLKM*IL 273
MSLMKEFMMDMALEEMPMSGCTC TLKM*IL
Sbjct: 175 MSLMKEFMMDMALEEMPMSGCTCLRTLKM*IL 80
>gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_A08_B15, mRNA sequence
Length = 655
Score = 202 bits (436), Expect = 6e-053
Identities = 88/89 (98%), Positives = 89/89 (100%)
Frame = +1 / +3
Query: 262 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 441
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSRE
Sbjct: 81 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 260
Query: 442 IQTSVRLVLPGELAKHAVSEGTKAVTKFT 528
IQTSVRLVLPGELAKHAVSEGTKAVTKFT
Sbjct: 261 IQTSVRLVLPGELAKHAVSEGTKAVTKFT 347
Score = 159 bits (341), Expect = 7e-040
Identities = 70/90 (77%), Positives = 73/90 (81%)
Frame = -1 / -1
Query: 536 *EEVNLVTALVPSETACLASSPGSTRRTEVWISRDVMVGFXXXXXXXXXXXXXXSKMSLM 357
*+EVN VTALVPS+TACLASSPG TRRTEVWISR+VMVGF SKMSLM
Sbjct: 355 *DEVNFVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLLYLASFADSPASFSKMSLM 176
Query: 356 KEFMMDMALEEMPMSGCTCFSTLKM*ILYV 267
KEFMMDMALEEMPMSGCTCF TLKM*ILYV
Sbjct: 175 KEFMMDMALEEMPMSGCTCFRTLKM*ILYV 86
>gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_B07_C14, mRNA
sequence
Length = 454
Score = 202 bits (436), Expect = 6e-053
Identities = 88/89 (98%), Positives = 89/89 (100%)
Frame = +1 / +1
Query: 262 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 441
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSRE
Sbjct: 13 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 192
Query: 442 IQTSVRLVLPGELAKHAVSEGTKAVTKFT 528
IQTSVRLVLPGELAKHAVSEGTKAVTKFT
Sbjct: 193 IQTSVRLVLPGELAKHAVSEGTKAVTKFT 279
Score = 152 bits (326), Expect = 9e-038
Identities = 68/92 (73%), Positives = 72/92 (78%)
Frame = -1 / -3
Query: 548 SVQA*EEVNLVTALVPSETACLASSPGSTRRTEVWISRDVMVGFXXXXXXXXXXXXXXSK 369
+ + *E+VN VTALVPSETACLASSPG TRRTEVWISR+VMVGF SK
Sbjct: 299 TTRT*EDVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFLL*RASLADSPASFSK 120
Query: 368 MSLMKEFMMDMALEEMPMSGCTCFSTLKM*IL 273
MSLMKEFMMDMALEEMPMSGCTC TLKM*IL
Sbjct: 119 MSLMKEFMMDMALEEMPMSGCTCLRTLKM*IL 24
>gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4408_D06_H12, mRNA
sequence
Length = 415
Score = 202 bits (436), Expect = 6e-053
Identities = 88/89 (98%), Positives = 89/89 (100%)
Frame = +1 / +2
Query: 262 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 441
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSRE
Sbjct: 41 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 220
Query: 442 IQTSVRLVLPGELAKHAVSEGTKAVTKFT 528
IQTSVRLVLPGELAKHAVSEGTKAVTKFT
Sbjct: 221 IQTSVRLVLPGELAKHAVSEGTKAVTKFT 307
Score = 149 bits (321), Expect = 4e-037
Identities = 69/89 (77%), Positives = 72/89 (80%)
Frame = -1 / -2
Query: 539 A*EEVNLVTALVPSETACLASSPGSTRRTEVWISRDVMVGFXXXXXXXXXXXXXXSKMSL 360
A*E+VNLVTALVPSETACLASSPG TRRTEV ISR+VMVGF SKMSL
Sbjct: 318 A*EDVNLVTALVPSETACLASSPGRTRRTEVCISREVMVGFLL*RASLADSPASFSKMSL 139
Query: 359 MKEFMMDMALEEMPMSGCTCFSTLKM*IL 273
MKEFMMDMALE+MPMSGCTC STLKM*IL
Sbjct: 138 MKEFMMDMALEDMPMSGCTCLSTLKM*IL 52
>gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4516_H02_P04, mRNA sequence
Length = 472
Score = 200 bits (432), Expect = 2e-052
Identities = 87/88 (98%), Positives = 88/88 (100%)
Frame = +1 / +2
Query: 265 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSREI 444
ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSREI
Sbjct: 11 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREI 190
Query: 445 QTSVRLVLPGELAKHAVSEGTKAVTKFT 528
QTSVRLVLPGELAKHAVSEGTKAVTKFT
Sbjct: 191 QTSVRLVLPGELAKHAVSEGTKAVTKFT 274
Score = 152 bits (327), Expect = 6e-038
Identities = 72/109 (66%), Positives = 78/109 (71%)
Frame = -1 / -2
Query: 599 LTTTQGEHQHKRSLQRASVQA*EEVNLVTALVPSETACLASSPGSTRRTEVWISRDVMVG 420
+T Q + + R R +*EEVNLVTALVPSETACLASSPGSTRRTEVWIS +VMVG
Sbjct: 345 VTWNQHQSEQDRVFSRDIRTS*EEVNLVTALVPSETACLASSPGSTRRTEVWISLEVMVG 166
Query: 419 FXXXXXXXXXXXXXXSKMSLMKEFMMDMALEEMPMSGCTCFSTLKM*IL 273
F SK+SLMKEFMMDMALEEMPMSG TC TLKM*IL
Sbjct: 165 FLLYLASLADSPASFSKISLMKEFMMDMALEEMPMSGWTCLRTLKM*IL 19
>gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_C10_F20, mRNA sequence
Length = 360
Score = 153 bits (328), Expect = 5e-038
Identities = 68/70 (97%), Positives = 69/70 (98%)
Frame = +1 / +2
Query: 319 GISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS 498
GISS AMSIMNSFINDIFEKLAGE+AKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS
Sbjct: 11 GISS*AMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS 190
Query: 499 EGTKAVTKFT 528
EGTKAVTKFT
Sbjct: 191 EGTKAVTKFT 220
Score = 115 bits (245), Expect = 1e-026
Identities = 54/75 (72%), Positives = 58/75 (77%)
Frame = -1 / -1
Query: 539 A*EEVNLVTALVPSETACLASSPGSTRRTEVWISRDVMVGFXXXXXXXXXXXXXXSKMSL 360
A*E+VNLVTALVPSETACLASSPG TRRTEV ISR+VMVGF SKMSL
Sbjct: 231 A*EDVNLVTALVPSETACLASSPGRTRRTEVCISREVMVGFLL*RASLADSPASFSKMSL 52
Query: 359 MKEFMMDMALEEMPM 315
MKEFMMDMA E+MP+
Sbjct: 51 MKEFMMDMA*EDMPL 7
>gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_F01_K01, mRNA sequence
Length = 361
Score = 113 bits (241), Expect = 5e-026
Identities = 50/52 (96%), Positives = 51/52 (98%)
Frame = +1 / +3
Query: 373 EKLAGEAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFT 528
EKLA E+AKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFT
Sbjct: 12 EKLACESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFT 167
>gb|CN816143.1|CN816143 HRO4517_E01_I01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_E01_I01, mRNA sequence
Length = 384
Score = 87.7 bits (185), Expect(2) = 1e-022
Identities = 38/38 (100%), Positives = 38/38 (100%)
Frame = +1 / +2
Query: 415 KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFT 528
KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFT
Sbjct: 56 KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFT 169
Score = 33.6 bits (67), Expect(2) = 1e-022
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = +1 / +3
Query: 370 FEKLAGEAAKLARYNKKP 423
FEKL+GE+AKLAR+ + P
Sbjct: 12 FEKLSGESAKLARFTRSP 65
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,601,675
Number of Sequences: 8143
Number of extensions: 56408
Number of successful extensions: 499
Number of sequences better than 1.0e-020: 9
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 294595796
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)