TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.226
(818 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Aven... 183 3e-047
gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Aven... 183 3e-047
gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Aven... 182 9e-047
gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Aven... 182 9e-047
gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Aven... 182 9e-047
gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Aven... 180 3e-046
gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Aven... 132 7e-032
gb|CN816143.1|CN816143 HRO4517_E01_I01ZS5 Lib AA071E1X Aven... 94 3e-022
gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Aven... 100 4e-022
>gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4507_C01_F01, mRNA sequence
Length = 365
Score = 183 bits (394), Expect = 3e-047
Identities = 82/93 (88%), Positives = 83/93 (89%)
Frame = -2 / +3
Query: 550 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSRE 371
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSRE
Sbjct: 69 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 248
Query: 370 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*I 272
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*+
Sbjct: 249 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*V 347
Score = 109 bits (233), Expect = 5e-025
Identities = 45/56 (80%), Positives = 46/56 (82%)
Frame = -1 / +2
Query: 545 DLQDLHLQGAQAGAPRHRHLVQGHVHHEFLHQRHLREARRGGRQARPLQQEAHHHL 378
DLQDLHLQG QAGAP HRHL+QGHVHHE LHQRHL E R QARPL QEAHHHL
Sbjct: 74 DLQDLHLQGPQAGAPGHRHLLQGHVHHELLHQRHL*ETRWRVCQARPL*QEAHHHL 241
Score = 84.0 bits (177), Expect(2) = 9e-032
Identities = 35/39 (89%), Positives = 37/39 (94%)
Frame = +3 / -2
Query: 276 *DEVNLVTAFVPSETACLASSPGRTRRTEVWISREVMVG 392
*++VN VTA VPSETACLASSPGRTRRTEVWISREVMVG
Sbjct: 343 *EDVNFVTALVPSETACLASSPGRTRRTEVWISREVMVG 227
Score = 68.0 bits (142), Expect(2) = 9e-032
Identities = 29/31 (93%), Positives = 30/31 (96%)
Frame = +3 / -2
Query: 459 EFMMDMALDEMPMSGCTCLSTLKM*IL*VST 551
EFMMDMAL+EMPMSGCTCL TLKM*IL*VST
Sbjct: 160 EFMMDMALEEMPMSGCTCLRTLKM*IL*VST 68
>gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_B07_C14, mRNA
sequence
Length = 454
Score = 183 bits (394), Expect = 3e-047
Identities = 82/93 (88%), Positives = 83/93 (89%)
Frame = -2 / +1
Query: 550 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSRE 371
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSRE
Sbjct: 13 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 192
Query: 370 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*I 272
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*+
Sbjct: 193 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*V 291
Score = 120 bits (256), Expect = 4e-028
Identities = 48/56 (85%), Positives = 49/56 (87%)
Frame = -1 / +3
Query: 545 DLQDLHLQGAQAGAPRHRHLVQGHVHHEFLHQRHLREARRGGRQARPLQQEAHHHL 378
DLQDLHLQG QAGAP HRHL+QGHVHHE LHQRHLREAR RQA PLQQEAHHHL
Sbjct: 18 DLQDLHLQGPQAGAPGHRHLLQGHVHHELLHQRHLREARWRVRQAGPLQQEAHHHL 185
Score = 84.0 bits (177), Expect(2) = 9e-032
Identities = 35/39 (89%), Positives = 37/39 (94%)
Frame = +3 / -3
Query: 276 *DEVNLVTAFVPSETACLASSPGRTRRTEVWISREVMVG 392
*++VN VTA VPSETACLASSPGRTRRTEVWISREVMVG
Sbjct: 287 *EDVNFVTALVPSETACLASSPGRTRRTEVWISREVMVG 171
Score = 68.0 bits (142), Expect(2) = 9e-032
Identities = 29/31 (93%), Positives = 30/31 (96%)
Frame = +3 / -3
Query: 459 EFMMDMALDEMPMSGCTCLSTLKM*IL*VST 551
EFMMDMAL+EMPMSGCTCL TLKM*IL*VST
Sbjct: 104 EFMMDMALEEMPMSGCTCLRTLKM*IL*VST 12
>gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_A08_B15, mRNA sequence
Length = 655
Score = 182 bits (391), Expect = 9e-047
Identities = 82/92 (89%), Positives = 82/92 (89%)
Frame = -2 / +3
Query: 550 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSRE 371
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSRE
Sbjct: 81 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 260
Query: 370 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 275
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 261 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 356
Score = 139 bits (299), Expect = 4e-034
Identities = 63/92 (68%), Positives = 65/92 (70%)
Frame = +3 / -1
Query: 276 *DEVNLVTAFVPSETACLASSPGRTRRTEVWISREVMVGXXXXXXXXXXXXXXXXXXXXX 455
*DEVN VTA VPS+TACLASSPGRTRRTEVWISREVMVG
Sbjct: 355 *DEVNFVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLLYLASFADSPASFSKMSLM 176
Query: 456 XEFMMDMALDEMPMSGCTCLSTLKM*IL*VST 551
EFMMDMAL+EMPMSGCTC TLKM*IL VST
Sbjct: 175 KEFMMDMALEEMPMSGCTCFRTLKM*ILYVST 80
Score = 105 bits (223), Expect(2) = 2e-024
Identities = 41/56 (73%), Positives = 46/56 (82%)
Frame = -1 / +2
Query: 545 DLQDLHLQGAQAGAPRHRHLVQGHVHHEFLHQRHLREARRGGRQARPLQQEAHHHL 378
D+QDLHLQG +AGA RHRHL+QGHVHHE LHQRHLRE R +A +QQEAHHHL
Sbjct: 86 DIQDLHLQGPEAGASRHRHLLQGHVHHELLHQRHLRETRWRVGKACEVQQEAHHHL 253
Score = 22.1 bits (42), Expect(2) = 2e-024
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = -1 / +2
Query: 308 HKGGHQVHFVL 276
H+G H+VH VL
Sbjct: 323 HQGCHEVHLVL 355
>gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4527_G09_N17, mRNA sequence
Length = 412
Score = 182 bits (391), Expect = 9e-047
Identities = 82/92 (89%), Positives = 82/92 (89%)
Frame = -2 / +1
Query: 550 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSRE 371
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSRE
Sbjct: 28 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 207
Query: 370 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 275
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 208 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 303
Score = 115 bits (246), Expect(2) = 6e-028
Identities = 46/56 (82%), Positives = 48/56 (85%)
Frame = -1 / +3
Query: 545 DLQDLHLQGAQAGAPRHRHLVQGHVHHEFLHQRHLREARRGGRQARPLQQEAHHHL 378
DLQDLHLQGA+AGAP HRHL+QGHVHHE LHQRHL E R GRQA LQQEAHHHL
Sbjct: 33 DLQDLHLQGAEAGAPGHRHLLQGHVHHELLHQRHL*ETRWRGRQAGALQQEAHHHL 200
Score = 87.2 bits (184), Expect(2) = 7e-033
Identities = 36/38 (94%), Positives = 37/38 (97%)
Frame = +3 / -3
Query: 279 DEVNLVTAFVPSETACLASSPGRTRRTEVWISREVMVG 392
DEVNLVTA VPS+TACLASSPGRTRRTEVWISREVMVG
Sbjct: 299 DEVNLVTALVPSDTACLASSPGRTRRTEVWISREVMVG 186
Score = 68.4 bits (143), Expect(2) = 7e-033
Identities = 29/31 (93%), Positives = 30/31 (96%)
Frame = +3 / -3
Query: 459 EFMMDMALDEMPMSGCTCLSTLKM*IL*VST 551
EFMMDMAL+EMPMSGCTC STLKM*IL*VST
Sbjct: 119 EFMMDMALEEMPMSGCTCFSTLKM*IL*VST 27
Score = 23.5 bits (45), Expect(2) = 6e-028
Identities = 7/12 (58%), Positives = 10/12 (83%)
Frame = -1 / +3
Query: 308 HKGGHQVHFVLD 273
H+G HQVH V++
Sbjct: 270 HQGRHQVHLVVE 305
>gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4408_D06_H12, mRNA
sequence
Length = 415
Score = 182 bits (391), Expect = 9e-047
Identities = 82/92 (89%), Positives = 82/92 (89%)
Frame = -2 / +2
Query: 550 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSRE 371
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSRE
Sbjct: 41 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 220
Query: 370 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 275
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 221 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 316
Score = 109 bits (232), Expect(2) = 1e-025
Identities = 45/56 (80%), Positives = 47/56 (83%)
Frame = -1 / +1
Query: 545 DLQDLHLQGAQAGAPRHRHLVQGHVHHEFLHQRHLREARRGGRQARPLQQEAHHHL 378
DLQDLHLQGAQAGAP HRH++QGHVHHE LH RHL EA G QAR LQQEAHHHL
Sbjct: 46 DLQDLHLQGAQAGAPGHRHILQGHVHHELLH*RHL*EACWGVCQARSLQQEAHHHL 213
Score = 79.9 bits (168), Expect(2) = 8e-031
Identities = 35/39 (89%), Positives = 37/39 (94%)
Frame = +3 / -2
Query: 276 *DEVNLVTAFVPSETACLASSPGRTRRTEVWISREVMVG 392
*++VNLVTA VPSETACLASSPGRTRRTEV ISREVMVG
Sbjct: 315 *EDVNLVTALVPSETACLASSPGRTRRTEVCISREVMVG 199
Score = 68.9 bits (144), Expect(2) = 8e-031
Identities = 29/31 (93%), Positives = 31/31 (100%)
Frame = +3 / -2
Query: 459 EFMMDMALDEMPMSGCTCLSTLKM*IL*VST 551
EFMMDMAL++MPMSGCTCLSTLKM*IL*VST
Sbjct: 132 EFMMDMALEDMPMSGCTCLSTLKM*IL*VST 40
Score = 22.1 bits (42), Expect(2) = 1e-025
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = -1 / +1
Query: 308 HKGGHQVHFVL 276
H+G HQVH +L
Sbjct: 283 HQGCHQVHILL 315
>gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4516_H02_P04, mRNA sequence
Length = 472
Score = 180 bits (387), Expect = 3e-046
Identities = 81/91 (89%), Positives = 81/91 (89%)
Frame = -2 / +2
Query: 547 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSREI 368
ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSREI
Sbjct: 11 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREI 190
Query: 367 QTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 275
QTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 191 QTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 283
Score = 132 bits (282), Expect = 9e-032
Identities = 62/91 (68%), Positives = 64/91 (70%)
Frame = +3 / -2
Query: 276 *DEVNLVTAFVPSETACLASSPGRTRRTEVWISREVMVGXXXXXXXXXXXXXXXXXXXXX 455
*+EVNLVTA VPSETACLASSPG TRRTEVWIS EVMVG
Sbjct: 282 *EEVNLVTALVPSETACLASSPGSTRRTEVWISLEVMVGFLLYLASLADSPASFSKISLM 103
Query: 456 XEFMMDMALDEMPMSGCTCLSTLKM*IL*VS 548
EFMMDMAL+EMPMSG TCL TLKM*IL*VS
Sbjct: 102 KEFMMDMALEEMPMSGWTCLRTLKM*IL*VS 10
Score = 105 bits (224), Expect(2) = 2e-025
Identities = 43/56 (76%), Positives = 46/56 (82%)
Frame = -1 / +1
Query: 545 DLQDLHLQGAQAGAPRHRHLVQGHVHHEFLHQRHLREARRGGRQARPLQQEAHHHL 378
DLQDLHLQG QAG PRHRHL+QGHVHHE LHQR+L EA QAR +QQEAHHHL
Sbjct: 13 DLQDLHLQGPQAGPPRHRHLLQGHVHHELLHQRYL*EAGWRVCQAREVQQEAHHHL 180
Score = 25.4 bits (49), Expect(2) = 2e-025
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -1 / +1
Query: 308 HKGGHQVHFVL 276
H+G HQVHF+L
Sbjct: 250 HQGRHQVHFLL 282
>gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_C10_F20, mRNA sequence
Length = 360
Score = 132 bits (283), Expect = 7e-032
Identities = 62/73 (84%), Positives = 62/73 (84%)
Frame = -2 / +2
Query: 493 GISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSREIQTSVRLVLPGELAKHAVS 314
GISS AMSIMNSFINDIF ARYNKKPTITSREIQTSVRLVLPGELAKHAVS
Sbjct: 11 GISS*AMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS 190
Query: 313 EGTKAVTKFTSS* 275
EGTKAVTKFTSS*
Sbjct: 191 EGTKAVTKFTSS* 229
>gb|CN816143.1|CN816143 HRO4517_E01_I01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_E01_I01, mRNA sequence
Length = 384
Score = 94.1 bits (199), Expect(2) = 3e-022
Identities = 42/46 (91%), Positives = 43/46 (93%)
Frame = -2 / +2
Query: 397 KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*IGCLP 260
KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*+ P
Sbjct: 56 KKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*VRFAP 193
Score = 25.8 bits (50), Expect(2) = 3e-022
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = -1 / +2
Query: 443 LREARRGGRQARPLQQE 393
LRE RG RQARPL ++
Sbjct: 11 LRETFRGVRQARPLYKK 61
>gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_F01_K01, mRNA sequence
Length = 361
Score = 100 bits (212), Expect = 4e-022
Identities = 44/44 (100%), Positives = 44/44 (100%)
Frame = -2 / +3
Query: 406 RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 275
RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 45 RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 176
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,461,284
Number of Sequences: 8143
Number of extensions: 67004
Number of successful extensions: 1476
Number of sequences better than 1.0e-020: 9
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 278770402
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)