TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.211
(777 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Aven... 184 2e-047
gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Aven... 183 3e-047
gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Aven... 182 6e-047
gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Aven... 182 8e-047
gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Aven... 182 8e-047
gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Aven... 180 3e-046
gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Aven... 134 1e-032
gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Aven... 102 8e-023
>gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4408_D06_H12, mRNA
sequence
Length = 415
Score = 184 bits (396), Expect = 2e-047
Identities = 83/94 (88%), Positives = 84/94 (89%)
Frame = -2 / +2
Query: 512 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSRE 333
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSRE
Sbjct: 41 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 220
Query: 332 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*AS 231
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*A+
Sbjct: 221 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*AA 322
Score = 144 bits (310), Expect = 1e-035
Identities = 68/93 (73%), Positives = 71/93 (76%)
Frame = +1 / -2
Query: 235 A*ELVNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRXXXXXXXXXXXXXXX 414
A*E VNLVTALVPSETACLASSPGRTRRTEV ISR+VMVGFLL R
Sbjct: 318 A*EDVNLVTALVPSETACLASSPGRTRRTEVCISREVMVGFLL*RASLADSPASFSKMSL 139
Query: 415 XXEFMMDMALDEMPMSGCTCFSTLKM*ILYVST 513
EFMMDMAL++MPMSGCTC STLKM*IL VST
Sbjct: 138 MKEFMMDMALEDMPMSGCTCLSTLKM*IL*VST 40
>gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4527_G09_N17, mRNA sequence
Length = 412
Score = 183 bits (394), Expect = 3e-047
Identities = 83/94 (88%), Positives = 83/94 (88%)
Frame = -2 / +1
Query: 512 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSRE 333
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSRE
Sbjct: 28 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 207
Query: 332 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*AS 231
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* S
Sbjct: 208 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*RS 309
Score = 151 bits (324), Expect = 1e-037
Identities = 67/89 (75%), Positives = 70/89 (78%)
Frame = +1 / -3
Query: 247 VNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRXXXXXXXXXXXXXXXXXEF 426
VNLVTALVPS+TACLASSPGRTRRTEVWISR+VMVGFLL R EF
Sbjct: 293 VNLVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLL*RASLAASPASFSKMSLMKEF 114
Query: 427 MMDMALDEMPMSGCTCFSTLKM*ILYVST 513
MMDMAL+EMPMSGCTCFSTLKM*IL VST
Sbjct: 113 MMDMALEEMPMSGCTCFSTLKM*IL*VST 27
>gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_A08_B15, mRNA sequence
Length = 655
Score = 182 bits (392), Expect = 6e-047
Identities = 82/93 (88%), Positives = 83/93 (89%)
Frame = -2 / +3
Query: 512 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSRE 333
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSRE
Sbjct: 81 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 260
Query: 332 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*A 234
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*+
Sbjct: 261 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*S 359
Score = 153 bits (329), Expect = 3e-038
Identities = 66/89 (74%), Positives = 69/89 (77%)
Frame = +1 / -1
Query: 247 VNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRXXXXXXXXXXXXXXXXXEF 426
VN VTALVPS+TACLASSPGRTRRTEVWISR+VMVGFLLY EF
Sbjct: 346 VNFVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLLYLASFADSPASFSKMSLMKEF 167
Query: 427 MMDMALDEMPMSGCTCFSTLKM*ILYVST 513
MMDMAL+EMPMSGCTCF TLKM*ILYVST
Sbjct: 166 MMDMALEEMPMSGCTCFRTLKM*ILYVST 80
>gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4507_C01_F01, mRNA sequence
Length = 365
Score = 182 bits (391), Expect = 8e-047
Identities = 82/92 (89%), Positives = 82/92 (89%)
Frame = -2 / +3
Query: 512 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSRE 333
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSRE
Sbjct: 69 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 248
Query: 332 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 237
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 249 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 344
Score = 146 bits (313), Expect = 5e-036
Identities = 67/92 (72%), Positives = 69/92 (75%)
Frame = +1 / -2
Query: 238 *ELVNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRXXXXXXXXXXXXXXXX 417
*E VN VTALVPSETACLASSPGRTRRTEVWISR+VMVGFLL R
Sbjct: 343 *EDVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFLL*RASLADSPASFSKMSLM 164
Query: 418 XEFMMDMALDEMPMSGCTCFSTLKM*ILYVST 513
EFMMDMAL+EMPMSGCTC TLKM*IL VST
Sbjct: 163 KEFMMDMALEEMPMSGCTCLRTLKM*IL*VST 68
>gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_B07_C14, mRNA
sequence
Length = 454
Score = 182 bits (391), Expect = 8e-047
Identities = 82/92 (89%), Positives = 82/92 (89%)
Frame = -2 / +1
Query: 512 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSRE 333
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSRE
Sbjct: 13 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 192
Query: 332 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 237
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 193 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 288
Score = 146 bits (313), Expect = 5e-036
Identities = 67/92 (72%), Positives = 69/92 (75%)
Frame = +1 / -3
Query: 238 *ELVNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRXXXXXXXXXXXXXXXX 417
*E VN VTALVPSETACLASSPGRTRRTEVWISR+VMVGFLL R
Sbjct: 287 *EDVNFVTALVPSETACLASSPGRTRRTEVWISREVMVGFLL*RASLADSPASFSKMSLM 108
Query: 418 XEFMMDMALDEMPMSGCTCFSTLKM*ILYVST 513
EFMMDMAL+EMPMSGCTC TLKM*IL VST
Sbjct: 107 KEFMMDMALEEMPMSGCTCLRTLKM*IL*VST 12
>gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4516_H02_P04, mRNA sequence
Length = 472
Score = 180 bits (387), Expect = 3e-046
Identities = 81/91 (89%), Positives = 81/91 (89%)
Frame = -2 / +2
Query: 509 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSREI 330
ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSREI
Sbjct: 11 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREI 190
Query: 329 QTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 237
QTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 191 QTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 283
Score = 137 bits (294), Expect = 2e-033
Identities = 64/92 (69%), Positives = 67/92 (72%)
Frame = +1 / -2
Query: 235 A*ELVNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRXXXXXXXXXXXXXXX 414
+*E VNLVTALVPSETACLASSPG TRRTEVWIS +VMVGFLLY
Sbjct: 285 S*EEVNLVTALVPSETACLASSPGSTRRTEVWISLEVMVGFLLYLASLADSPASFSKISL 106
Query: 415 XXEFMMDMALDEMPMSGCTCFSTLKM*ILYVS 510
EFMMDMAL+EMPMSG TC TLKM*IL VS
Sbjct: 105 MKEFMMDMALEEMPMSGWTCLRTLKM*IL*VS 10
>gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_C10_F20, mRNA sequence
Length = 360
Score = 134 bits (288), Expect = 1e-032
Identities = 63/75 (84%), Positives = 64/75 (85%)
Frame = -2 / +2
Query: 455 GISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSREIQTSVRLVLPGELAKHAVS 276
GISS AMSIMNSFINDIF ARYNKKPTITSREIQTSVRLVLPGELAKHAVS
Sbjct: 11 GISS*AMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS 190
Query: 275 EGTKAVTKFTSS*AS 231
EGTKAVTKFTSS*A+
Sbjct: 191 EGTKAVTKFTSS*AA 235
Score = 105 bits (225), Expect = 6e-024
Identities = 50/75 (66%), Positives = 54/75 (72%)
Frame = +1 / -1
Query: 235 A*ELVNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLYRXXXXXXXXXXXXXXX 414
A*E VNLVTALVPSETACLASSPGRTRRTEV ISR+VMVGFLL R
Sbjct: 231 A*EDVNLVTALVPSETACLASSPGRTRRTEVCISREVMVGFLL*RASLADSPASFSKMSL 52
Query: 415 XXEFMMDMALDEMPM 459
EFMMDMA ++MP+
Sbjct: 51 MKEFMMDMA*EDMPL 7
>gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_F01_K01, mRNA sequence
Length = 361
Score = 102 bits (217), Expect = 8e-023
Identities = 46/55 (83%), Positives = 48/55 (87%)
Frame = -2 / +3
Query: 368 RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*ASDATCTRLLF 204
RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* + + LF
Sbjct: 45 RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*TALTAWEKTLF 209
Score = 95.5 bits (202), Expect = 1e-020
Identities = 43/54 (79%), Positives = 44/54 (81%)
Frame = +1 / -1
Query: 205 KRRRVHVASLA*ELVNLVTALVPSETACLASSPGRTRRTEVWISRDVMVGFLLY 366
KR H *E VNLVTALVPSETACLASSPGRTRRTEVWIS +VMVGFLLY
Sbjct: 208 KRVFSHAVRAV*EEVNLVTALVPSETACLASSPGRTRRTEVWISLEVMVGFLLY 47
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,286,860
Number of Sequences: 8143
Number of extensions: 49667
Number of successful extensions: 656
Number of sequences better than 1.0e-020: 8
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 261727670
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)