TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 131537.2.203
         (959 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN821322.1|CN821322  HRO4424_A05_B10ZS5 Lib AA069E1X Aven...   100   3e-025

>gb|CN821322.1|CN821322 HRO4424_A05_B10ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
           leaf Avena sativa cDNA clone HRO4424_A05_B10, mRNA
           sequence
          Length = 457

 Score =  100 bits (212), Expect(2) = 3e-023
 Identities = 40/53 (75%), Positives = 46/53 (86%)
 Frame = +2 / -3

                                                                
Query: 335 AAAVDEHLERDGHVDGDVEHLGLDGGAKARRRGEPGDALHERAALDVGVADVH 493
           +A VDE  ER+ HV+G+VEHLGLDGGA+A RRGE GDALHERAALD+ VADVH
Sbjct: 161 SAVVDEQAERERHVEGEVEHLGLDGGAEAHRRGEAGDALHERAALDLAVADVH 3




 Score = 96.4 bits (204), Expect(2) = 3e-025
 Identities = 36/50 (72%), Positives = 46/50 (92%)
 Frame = -2 / +3

                                                             
Query: 493 VHVGDANVQCCPLVKGIAGLSAAACLCTAIKAKVLDISVYVPIALEVLVN 344
           VH+GD+ V+CC LV+G+AGL+AA CLCTAIKAKVLD+S+YVP+AL +LVN
Sbjct: 3   VHIGDSKVKCCSLVQGVAGLTAAVCLCTAIKAKVLDLSLYVPLALSLLVN 152




 Score = 34.1 bits (68), Expect(2) = 3e-025
 Identities = 10/12 (83%), Positives = 11/12 (91%)
 Frame = -2 / +3

                       
Query: 346 NCGCAVPPGYKC 311
           +CGCAVPPGY C
Sbjct: 153 DCGCAVPPGYTC 188




 Score = 23.5 bits (45), Expect(2) = 3e-023
 Identities = 9/16 (56%), Positives = 11/16 (68%)
 Frame = +3 / -1

                           
Query: 297 GIVQVHL*PGGTAQPQ 344
           G+    +*PGGTA PQ
Sbjct: 202 GMA*AQV*PGGTAHPQ 155



  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 8,875,269
Number of Sequences: 8143
Number of extensions: 123132
Number of successful extensions: 1102
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 335985288
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)